Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A0 F0 I1 R1
|
146 |
27.9 |
2327082 |
89.7% |
2087392 |
107.8 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
AM260479 |
564,366 |
C→G |
P73A (CCC→GCC) |
h16_A0537 → |
Uncharacterized protein conserved in bacteria |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | AM260479 | 564,366 | 0 | C | G | 100.0%
| 32.9
/ NA
| 10 | P73A (CCC→GCC) | h16_A0537 | Uncharacterized protein conserved in bacteria |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base G (4/6); total (4/6) |
ATGTGGTTTGCGCAGGGCCGACCGGTGCCGGCGGCCCGCCAGGCCGTCGATGCGCTGGCCGCGGCCGCGGCCGACGGCCTCGACCCGCGCGACTACGATGCCGACGCCTTGCGCCAGCCCGTGGCCCAGGCCGCCAGCGGGCCAGCATTGCCGCCGGACGCTGCCGCACGCGTGGATGCGGCGCTGAGCGCCGCGATGGAGCGGATGCTGGCAGACCTGCACGGCGGGCGCGTCAACCCGCGCGC > AM260479/564249‑564493
|
aTGTGGTTTGCGCAGGGCCGACCGGTGCCGGCGGCCCGCCAGGCCGTCGATGCGCTGGCCGCGGCCGCGGCCGACGGCCTCGACCCGCGCGACTACGATGCCGACGCCTTGCGCCAGGCCGt < 1:424765/122‑1 (MQ=255)
aTGTGGTTTGCGCAGGGCCGACCGGTGCCGGCGGCCCGCCAGGCCGTCGATGCGCTGGCCGCGGCCGCGGCCGACGGCCTCGACCCGCGCGACTACGATGCCGACGCCTTGCGCCAGGCCGt > 2:424765/1‑122 (MQ=255)
cgcAGGGCCGACCGGTGCCGGCGGCCCGCCAGGCCGTCGATGCGCTGGCCGCGGCCGCGGCCGACGGCCTCGACCCGCGCGACTACGATGCCGACGCCTTGCGCCAGGCCGTGGCCCAGGCCGCCAGCGGGCCa < 2:1107761/134‑1 (MQ=255)
gggCCGACCGGTGCCGGCGGCCCGCCAGGCCGTCGATGCGCTGGCCGCGGCCGCGGCCGACGGCCTCGACCCGCGCGACTACGATGCCGACGCCTTGCGCCAGGCCGTGGCCCAGGCCGCCAGCGGGCCAGCAt > 2:929771/1‑134 (MQ=255)
ggcggcCCGCCAGGCCGTCGATGCGCTGGCCGCGGCCGCGGCCGACGGCCTCGACCCGCGCGACTACGATGCCGACGCCTTGCGCCAGGCCGTGGCCCAGgccg < 1:193755/104‑1 (MQ=255)
ggcggcCCGCCAGGCCGTCGATGCGCTGGCCGCGGCCGCGGCCGACGGCCTCGACCCGCGCGACTACGATGCCGACGCCTTGCGCCAGGCCGTGGCCCAGgccg > 2:193755/1‑104 (MQ=255)
ccGCGCGACTACGATGCCGACGCCTTGCGCCAGGCCGt < 1:1148234/38‑1 (MQ=255)
ccGCGCGACTACGATGCCGACGCCTTGCGCCAGGCCGt > 2:1148234/1‑38 (MQ=255)
cgACTACGATGCCGACGCCTTGCGCCAGGCCGTGGCCCAGGCCGCCAGCGGGCCAGCATTGCCGCCGGACGCTGCCGCACGCGTGGATGCGGCGCTGAGCGCCGCGATGGAGCGGATGCTGGCAGACCTGCAcg < 1:320642/134‑1 (MQ=255)
cgcCAGGCCGTGGCCCAGGCCGCCAGCGGGCCAGCATTGCCGCCGGACGCTGCCGCACGCGTGGATGCGGCGCTGAGCGCCGCGATGGAGCGGATGCTGGCAGACCTGCACGGCGGGCGCGTCAACCcgcgcgc < 1:929771/134‑1 (MQ=255)
|
ATGTGGTTTGCGCAGGGCCGACCGGTGCCGGCGGCCCGCCAGGCCGTCGATGCGCTGGCCGCGGCCGCGGCCGACGGCCTCGACCCGCGCGACTACGATGCCGACGCCTTGCGCCAGCCCGTGGCCCAGGCCGCCAGCGGGCCAGCATTGCCGCCGGACGCTGCCGCACGCGTGGATGCGGCGCTGAGCGCCGCGATGGAGCGGATGCTGGCAGACCTGCACGGCGGGCGCGTCAACCCGCGCGC > AM260479/564249‑564493
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
BRESEQ :: bam2aln output
GGCCACGCCTGCCTTCGCCAACGAACCGATGTGGTTTGCGCAGGGCCGACCGGTGCCGGCGGCCCGCCAGGCCGTCGATGCGCTGGCCGCGGCCGCGGCCGACGGCCTCGACCCGCGCGACTACGATGCCGACGCCTTGCGCCAGCCCGTGGCCCAGGCCGCCAGCGGGCCAGCATTGCCGCCGGACGCTGCCGCACGCGTGGATGCGGCGCTGAGCGCCGCGATGGAGCGGATGCTGGCAGACCTGCACGGCGGGCGCGTCAACCCGCGCGCGGTCCATGCCAACTT > AM260479/564221‑564508
|
GGCCACGCCTGCCTTCGCCAACGAACCGATGTGGTTTGCGCAGGGCCGACCGGTGCCGGCGGCCCGCCAGGCCGTCGATGCGCTGGCCGCGGCCGCGGCCGACGGCCTCGACCCGCGCGACTACGATGCCGACGCCTTGCGCCAGGCCGT > GWNJ‑0478:712:GW2002102894th:2:2101:6190:81407/1‑150 (MQ=60)
gtaccgttttttttttcaagcagaagacggcatacgagattagcgagtgtctcgtgggctcggagatgtgtataagagacaGGCCGACGGCCTCGACCCGCGCGACTACGATGCCGACGCCTTGCGCCAGGCCGTGGCCCAGGCCGCCAG < GWNJ‑0478:712:GW2002102894th:2:2216:4393:28917/69‑1 (MQ=60)
CGCCAACGAACCGATGTGGTTTGCGCAGGGCCGACCGGTGCCGGCGGCCCGCCAGGCCGTCGATGCGCTGGCCGCGGCCGCGGCCGACGGCCTCGACCCGCGCGACTACGATGCCGACGCCTTGCGCCAGGCCGTGGCCCAGGCCGCCAG < GWNJ‑0478:712:GW2002102894th:2:1209:18761:17274/150‑1 (MQ=60)
gtgtataagagacagGGGCCGACCGGTGCCGGCGGCCCGCCAGGCCGTCGATGCGCTGGCCGCGGCCGCGGCCGACGGCCTCGACCCGCGCGACTACGATGCCGACGCCTTGCGCCAGGCCGTGGCCCAGGCCGCCAGCGGGCCAGCAT < GWNJ‑0478:712:GW2002102894th:2:1113:17660:26323/134‑1 (MQ=60)
tagcgagtgtctcgtgggctcggagatgtgtataagagacagGCGCCAGGCCGTGGCCCAGGCCGCCAGCGGGCCAGCATTGCCGCCGGACGCTGCCGCACGCGTGGATGCGGCGCTGAGCGCCGCGATGGAGCGGATGCTGGCAGACC < GWNJ‑0478:712:GW2002102894th:2:1102:16478:40741/107‑1 (MQ=60)
GCGACTACGATGCCGACGCCTTGCGCCAGGCCGTGGCCCAGGCCGCCAGCGGGCCAGCATTGCCGCCGGACGCTGCCGCACGCGTGGATGCGGCGCTGAGCGCCGCGATGGAGCGGATGCTGGCAGACCTGCACGGCGGGCGCGTCAACC < GWNJ‑0478:712:GW2002102894th:2:1202:16789:12331/150‑1 (MQ=60)
GCGCCAGGCCGTGGCCCAGGCCGCCAGCGGGCCAGCATTGCCGCCGGACGCTGCCGCACGCGTGGATGCGGCGCTGAGCGCCGCGATGGAGCGGATGCTGGCAGACCTGCACGGCGGGCGCGTCAACCCGCGCGCGGTCCATGCCAACTT < GWNJ‑0478:712:GW2002102894th:2:2204:7789:73817/150‑1 (MQ=60)
|
GGCCACGCCTGCCTTCGCCAACGAACCGATGTGGTTTGCGCAGGGCCGACCGGTGCCGGCGGCCCGCCAGGCCGTCGATGCGCTGGCCGCGGCCGCGGCCGACGGCCTCGACCCGCGCGACTACGATGCCGACGCCTTGCGCCAGCCCGTGGCCCAGGCCGCCAGCGGGCCAGCATTGCCGCCGGACGCTGCCGCACGCGTGGATGCGGCGCTGAGCGCCGCGATGGAGCGGATGCTGGCAGACCTGCACGGCGGGCGCGTCAACCCGCGCGCGGTCCATGCCAACTT > AM260479/564221‑564508
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
