Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A0 F0 I1 R1
|
146 |
27.9 |
2327082 |
89.7% |
2087392 |
107.8 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
AM260479 |
1,231,226 |
2 bp→CA |
intergenic (+63/‑151) |
glnE → / → h16_A1128 |
glutamine synthetase adenylyltransferase/Membrane protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | AM260479 | 1,231,226 | 0 | A | C | 100.0%
| 85.7
/ NA
| 23 | intergenic (+63/‑152) | glnE/h16_A1128 | glutamine synthetase adenylyltransferase/Membrane protein |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base C (9/14); total (9/14) |
| * | AM260479 | 1,231,227 | 0 | C | A | 100.0%
| 85.2
/ NA
| 23 | intergenic (+64/‑151) | glnE/h16_A1128 | glutamine synthetase adenylyltransferase/Membrane protein |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base A (9/14); total (9/14) |
ATTCACGGGTGCAGTACCTGGTGCTGCTGCACAACCCGGGCGCACCCGCAGCTGACGCGCAACGCGGGGCAATATCGCGCTGCTGCGCGCAGCGGGCGAGCTGGGCCTGATCGACGCACCGCTGGCACTGGCGGTGGGCGATGCCTACCGGCTGTTCCGCGCGCGCCAGCACCAGCTGCGGCTCGAGGGGCAGGCGCATGCGCGCGTCGCGCCCGAGTCGGTGGCGCAGCAGACCGGACAGG > AM260479/1231109‑1231350
||
aTTCAC‑GGTGCAGTACCTGGTGCTGCTGCAC‑AGCCGGGCGCACCCGCAGCTGACGCGCAACGC‑GGGCAATATCGCGCTGCTGCGCGCAGCGGGCGAGCTGGGCCTGATCGACGCCACGCTGGCACTGGCGGTgg < 2:873522/134‑1 (MQ=255)
aTTCAC‑GGTGCAGTACCTGGTGCTGCTGCAC‑AGCCGGGCGCACCCGCAGCTGACGCGCAACGC‑GGGCAATATCGCGCTGCTGCGCGCAGCGGGCGAGCTGGGCCTGATCGACGCCACGCTGGCACTGGCGGTgg < 2:69111/134‑1 (MQ=255)
cggTGCAGTACCTGGTGCTGCTGCAC‑AGCCGGGCGCACCCGCAGCTGACGCGCAACGC‑GGGCAATATCGCGCTGCTGCGCGCAGCGGGCGAGCTGGGCCTGATCGACGCCACGCTGGCACTGGCGGTGGGCGAt < 1:652824/133‑1 (MQ=255)
ctgctgcAC‑AGCCGGGCGCACCCGCAGCTGACGCGCAACGC‑GGGCAATATCGCGCTGCTGCGCGCAGCGGGCGAGCTGGGCCTGATCGACGCCACGCTGGCACTGGCGGTgg < 2:847163/112‑1 (MQ=255)
ctgctgcAC‑AGCCGGGCGCACCCGCAGCTGACGCGCAACGC‑GGGCAATATCGCGCTGCTGCGCGCAGCGGGCGAGCTGGGCCTGATCGACGCCACGCTGGCACTGGCGGTgg > 1:847163/1‑112 (MQ=255)
ctgctgcAC‑AGCCGGGCGCACCCGCAGCTGACGCGCAACGC‑GGGCAATATCGCGCTGCTGCGCGCAGCGGGCGAGCTGGGCCTGATCGACGCCACGCTGGCACTGGCGGTGGGCGATGCCTACCGGCTGTTc < 2:858025/132‑1 (MQ=255)
ctgctgcAC‑AGCCGGGCGCACCCGCAGCTGACGCGCAACGC‑GGGCAATATCGCGCTGCTGCGCGCAGCGGGCGAGCTGGGCCTGATCGACGCCACGCTGGCACTGGCGGTGGGCGATGCCTACCGGCTGTTc > 1:858025/1‑132 (MQ=255)
acagccGGGCGCACCCGCAGCTGACGCGCAACGC‑GGGCAATATCGCGCTGCTGCGCGCATCGGGCGAGCTGGGCCTGATCGACGCCACGCTGGCACTGGCGGTGGGCGATGCCTACCGGCTGTTCCgcgcgcgc > 1:269495/5‑134 (MQ=255)
gcACCCGCAGCTGACGCGCAACGC‑GGGCAATATCGCGCTGCTGCGCGCAGCGGGCGAGCTGGGCCTGATCGACGCCACGCTGGCACTGGCGGTGGGCGATGCCTACCGGCTGTTcc > 2:380685/1‑116 (MQ=255)
gcACCCGCAGCTGACGCGCAACGC‑GGGCAATATCGCGCTGCTGCGCGCAGCGGGCGAGCTGGGCCTGATCGACGCCACGCTGGCACTGGCGGTGGGCGATGCCTACCGGCTGTTcc < 1:380685/116‑1 (MQ=255)
cGCAGCTGACGCGCAACGC‑GGGCAATATCGCGCTGCTGCGCGCAGCGGGCGAGCTGGGCCTGATCGACGCCACGCTGGCACTGGCGGTGGGCGATGCCTACCGGCTGTTCCGCGCGCGCCAGCACCAGCTGCgg < 2:873386/134‑1 (MQ=255)
aCGCGCAACGC‑GGGCAATATCGCGCTGCTGCGCGCATCGGGCGAGCTGGGCCTGATCGACGCCACGCTGGCACTGGCGGTGGGCGATGCCTACCGGCTGTTCCGCGCGCGCCAGCACCAGCTGCGGCTCGAgg < 2:269495/133‑1 (MQ=255)
cgcgCTGCTGCGCGCAGCGGGCGAGCTGGGCCTGATCGACGCCACGCTGGCACTGGCGGTGGGCGATGCCTACCGGCTGTTCCGCGCGCGCCAGCACCAGCTGTGGCTCGAg > 2:65610/1‑112 (MQ=255)
cgcgCTGCTGCGCGCAGCGGGCGAGCTGGGCCTGATCGACGCCACGCTGGCACTGGCGGTGGGCGATGCCTACCGGCTGTTCCGCGCGCGCCAGCACCAGCTGTGGCTCGAg < 1:65610/112‑1 (MQ=255)
gcgcAGCGGGCGAGCTGGGCCTGATCGACGCCACGCTGGCACTGGCGGTGGGCGATGCCTACCGGCTGTTCCGCGCGCGCCAGCACCAGCTGCGGCTCGAg > 1:659174/1‑101 (MQ=255)
gcgcAGCGGGCGAGCTGGGCCTGATCGACGCCACGCTGGCACTGGCGGTGGGCGATGCCTACCGGCTGTTCCGCGCGCGCCAGCACCAGCTGCGGCTCGAg < 2:659174/101‑1 (MQ=255)
aGCGGGCGAGCTGGGCCTGATCGACGCCACGCTGGCACTGGCGGTGGGCGATGCCTACCGGCTGTTCCGCGCGCGCCAGCACCAGCTGCGGCTCGAGGGGCAGGCGCATGCGCGCGTCGCGCCCGAGTCGGTgg < 1:888391/134‑1 (MQ=255)
gCGGGCGAGCTGGGCCTGATCGACGCCACGCTGGCACTGGCGGTGGGCGATGCCTACCGGCTGTTCCGCGCGCGCCAGCACCAGCTGCGGCTCGAGGGGCAGGCGCATGCGCGCGTCGCGCCCGAGTCGGTGgc < 1:1045780/134‑1 (MQ=255)
ggCGAGCTGGGCCTGATCGACGCCACGCTGGCACTGGCGGTGGGCGATGCCTACCGGCTGTTCCGCGCGCGCCAGCACCAGCTGCGGCTCGAGGGGCAGGCGCATGCGCGCGTCGCGCCCGAGTc < 1:692739/125‑1 (MQ=255)
ggCGAGCTGGGCCTGATCGACGCCACGCTGGCACTGGCGGTGGGCGATGCCTACCGGCTGTTCCGCGCGCGCCAGCACCAGCTGCGGCTCGAGGGGCAGGCGCATGCGCGCGTCGCGCCCGAGTc > 2:692739/1‑125 (MQ=255)
cGAGCTGGGCCTGATCGACGCCACGCTGGCACTGGCGGTGGGCGATGCCTACCGGCTGTTCCGCGCGCGCCAGCACCAGCTGCGGCTCGAGGGGCAGGCGCATGCGCGCGTCGCGCCCGAGTCGGTGGCgcag > 1:840240/1‑133 (MQ=255)
cGAGCTGGGCCTGATCGACGCCACGCTGGCACTGGCGGTGGGCGATGCCTACCGGCTGTTCCGCGCGCGCCAGCACCAGCTGCGGCTCGAGGGGCAGGCGCATGCGCGCGTCGCGCCCGAGTCGGTGGCgcag > 2:512486/1‑133 (MQ=255)
aTCGACGCCACGCTGGCACTGGCGGTGGGCGATGCCTACCGGCTGTTCCGCGCGCGCCAGCACCAGCTGCGGCTCGAGGGGCAGGCGCATGCGCGCGTCGCGCCCGAGTCGGTGGCGCAGCAGACCGGACAgg < 1:512486/133‑1 (MQ=255)
||
ATTCACGGGTGCAGTACCTGGTGCTGCTGCACAACCCGGGCGCACCCGCAGCTGACGCGCAACGCGGGGCAATATCGCGCTGCTGCGCGCAGCGGGCGAGCTGGGCCTGATCGACGCACCGCTGGCACTGGCGGTGGGCGATGCCTACCGGCTGTTCCGCGCGCGCCAGCACCAGCTGCGGCTCGAGGGGCAGGCGCATGCGCGCGTCGCGCCCGAGTCGGTGGCGCAGCAGACCGGACAGG > AM260479/1231109‑1231350
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
BRESEQ :: bam2aln output
CATGGTCGATATCGAATTCACGGGTGCAGTACCTGGTGCTGCTGCACAACCCGGGCGCACCCGCAGCTGACGCGCAACGCGGGGCAATATCGCGCTGCTGCGCGCAGCGGGCGAGCTGGGCCTGATCGACGCACCGCTGGCACTGGCGGTGGGCGATGCCTACCGGCTGTTCCGCGCGCGCCAGCACCAGCTGCGGCTCGAGGGGCAGGCGCATGCGCGCGTCGCGCCCGAGTCGGTGGCGCAGCAGACCGGACAGGTGCGCGCGCTGTGGC > AM260479/1231094‑1231365
|
tttcttttttttttttcaagcagaagacggcatacgagattagcgagtgtctcgtgggctcggagatgtgtataagagacaGGGCAATATCGCGCTGCTGCGCGCAGCGGGCGAGCTGGGCCTGATCGACGCCACGCTGGCACTGGC < GWNJ‑0478:712:GW2002102894th:2:1207:20984:99910/66‑1 (MQ=60)
acGGTGCAGTACCTGGTGCTGCTGCAC‑AGCCGGGCGCACCCGCAGCTGACGCGCAACGC‑GGGCAATATCGCGCTGCTGCGCGCAGCGGGCGAGCTGGGCCTGATCGACGCCACGCTGGCACTGGCGGTGGGCGATGCCTACCGGCTGTTC < GWNJ‑0478:712:GW2002102894th:2:2104:18740:39154/148‑1 (MQ=60)
GTGCAGTACCTGGTGCTGCTGCAC‑AGCCGGGCGCACCCGCAGCTGACGCGCAACGC‑GGGCAATATCGCGCTGCTGCGCGCAGCGGGCGAGCTGGGCCTGATCGACGCCACGCTGGCACTGGCGGTGGGCGATGCCTACCGGCctgtctct > GWNJ‑0478:712:GW2002102894th:2:2114:7815:87281/1‑142 (MQ=60)
GTGCAGTACCTGGTGCTGCTGCAC‑AGCCGGGCGCACCCGCAGCTGACGCGCAACGC‑GGGCAATATCGCGCTGCTGCGCGCAGCGGGCGAGCTGGGCCTGATCGACGCCACGCTGGCACTGGCGGTGGGCGATGCCTACCGGCTGTTCCGC > GWNJ‑0478:712:GW2002102894th:2:2116:20173:40435/1‑150 (MQ=60)
ACCTGGTGCTGCTGCAC‑AGCCGGGCGCACCCGCAGCTGACGCGCAACGC‑GGGCAATATCGCGCTGCTGCGCGCATCGGGCGAGCTGGGCCTGATCGACGCCACGCTGGCACTGGCGGTGGGCGATGCCTACCGGCTGTTCCGCGCGCGCC > GWNJ‑0478:712:GW2002102894th:2:1116:15096:7320/1‑150 (MQ=60)
acaggctgcacagCCGGGCGCACCCGCAGCTGACGCGCAACGC‑GGGCAATATCGCGCTGCTGCGCGCAGCGGGCGAGCTGGGCCTGATCGACGCCACGCTGGCACTGGCGGTGGGCGATGCCTACCGGCTGTTCCGCGCGCGCCAGCACC < GWNJ‑0478:712:GW2002102894th:2:1207:2878:69874/137‑1 (MQ=60)
GCTGCAC‑AGCCGGGCGCACCCGCAGCTGACGCGCAACGC‑GGGCAATATCGCGCTGCTGCGCGCAGCGGGCGAGCTGGGCCTGATCGACGCCACGCTGGCACctgtctcttatacacatctccgagcccacgagacactcgctaatctcgt > GWNJ‑0478:712:GW2002102894th:2:1202:6808:64386/1‑101 (MQ=60)
agagacagcaacgcGGGCAATATCGCGCTGCTGCGCGCAGCGGGCGAGCTGGGCCTGATCGACGCCACGCTGGCACTGGCGGTGGGCGATGCCTACCGGCTGTTCCGCGCGCGCCAGCACCAGCTGTGGCTCGAGGGGCAGGCGCATGCG < GWNJ‑0478:712:GW2002102894th:2:1104:19242:9690/136‑1 (MQ=60)
gcgcgatattgcccgcgttgcgcgtcagctGCGGGCGAGCTGGGCCTGATCGACGCCACGCTGGCACTGGCGGTGGGCGATGCCTACCGGCTGTTCCGCGCGCacctgtccggtctgctgcgccaccgactcgggcgcgacgcgcgcatg > GWNJ‑0478:712:GW2002102894th:2:2111:8782:38140/31‑103 (MQ=60)
ATATCGCGCTGCTGCGCGCAGCGGGCGAGCTGGGCCTGATCGACGCCACGCTGGCACTGGCGGTGGGCGATGCCTACCGGCTGTTCCGCGCGCGCCAGCACCAGCTGCGGCTCGAGGGGCAGGCGCATGCGCtgtctcttatacacatct > GWNJ‑0478:712:GW2002102894th:2:2104:8150:78161/1‑132 (MQ=60)
GCTGCGCGCAGCGGGCGAGCTGGGCCTGATCGACGCCACGCTGGCACTGGCGGTGGGCGATGCCTACCGGCTGTTCCGCGCGCGCCAGCACCAGCTGCGGCTCGAGGGGCAGGCGCATGCGCGCGTCGCGCCCGAGTCGGTGGCGCAGC > GWNJ‑0478:712:GW2002102894th:2:2114:19807:52026/1‑149 (MQ=60)
GCGCGCAGCGGGCGAGCTGGGCCTGATCGACGCCACGCTGGCACTGGCGGTGGGCGATGCCTACCGGCTGTTCCGCGCGCGCCAGCACCAGCTGCGGCTCGAGGGGCAGGCGCATGCGCGCGTCGCGCCCGAGTCGGTGGCGCAGCAGAC < GWNJ‑0478:712:GW2002102894th:2:2106:7414:78011/150‑1 (MQ=60)
CAGCGGGCGAGCTGGGCCTGATCGACGCCACGCTGGCACTGGCGGTGGGCGATGCCTACCGGCTGTTCCGCGCGCGCCAGCACCAGCTGCGGCTCGAGGGGCAGGCGCATGCGCGCGTCGCGCCCGAGTCGGTGGCGCAGCAGACCGGAC < GWNJ‑0478:712:GW2002102894th:2:2203:12206:19136/150‑1 (MQ=60)
AGCGGGCGAGCTGGGCCTGATCGACGCCACGCTGGCACTGGCGGTGGGCGATGCCTACCGGCTGTTCCGCGCGCGCCAGCACCAGCTGCGGCTCGAGGGGCAGGCGCATGCGCGCGTCGCGCCCGAGTCGGTGGCGCAGCAGACCGGACA < GWNJ‑0478:712:GW2002102894th:2:2210:20176:24177/150‑1 (MQ=60)
AGCTGGGCCTGATCGACGCCACGCTGGCACTGGCGGTGGGCGATGCCTACCGGCTGTTCCGCGCGCGCCAGCACCAGCTGCGGCTCGAGGctgtctcttatacacatctccgagcccacgagacactcgctaatctcgtatgccgtcttc > GWNJ‑0478:712:GW2002102894th:2:1212:19739:69446/1‑90 (MQ=60)
GGGCCTGATCGACGCCACGCTGGCACTGGCGGTGGGCGATGCCTACCGGCTGTTCCGCGCGCGCCAGCACCAGCTGCGGCTCGAGGGGCAGGCGCATGCGCGCGTCGCGCCCGAGTCGGTGGCGCAGCAGACCGGACAGGTGCtgtctct > GWNJ‑0478:712:GW2002102894th:2:1111:13856:88426/1‑143 (MQ=60)
GGCCTGATCGACGCCACGCTGGCACTGGCGGTGGGCGATGCCTACCGGCTGTTCCGCGCGCGCCAGCACCAGCTGCGGCTCGAGGGGCAGGCGCATGCGCGCGTCGCGCCCGAGTCGGTGGCGCAGCAGACCGGACAGGTGCGCGCGCTG > GWNJ‑0478:712:GW2002102894th:2:2113:20299:89420/1‑150 (MQ=60)
GATCGACGCCACGCTGGCACTGGCGGTGGGCGATGCCTACCGGCTGTTCCGCGCGCGCCAGCACCAGCTGCGGCTCGAGGGGCAGGCGCATGCGCGCGTCGCGCCCGAGTCGGTGGCGCAGCAGACCGGACAGGTGCGCGCGCTGTGGC < GWNJ‑0478:712:GW2002102894th:2:1212:10835:63868/149‑1 (MQ=60)
|
CATGGTCGATATCGAATTCACGGGTGCAGTACCTGGTGCTGCTGCACAACCCGGGCGCACCCGCAGCTGACGCGCAACGCGGGGCAATATCGCGCTGCTGCGCGCAGCGGGCGAGCTGGGCCTGATCGACGCACCGCTGGCACTGGCGGTGGGCGATGCCTACCGGCTGTTCCGCGCGCGCCAGCACCAGCTGCGGCTCGAGGGGCAGGCGCATGCGCGCGTCGCGCCCGAGTCGGTGGCGCAGCAGACCGGACAGGTGCGCGCGCTGTGGC > AM260479/1231094‑1231365
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
