Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A0 F0 I1 R1
|
146 |
27.9 |
2327082 |
89.7% |
2087392 |
107.8 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
AM260479 |
3,248,045 |
Δ1 bp |
coding (809/813 nt) |
h16_A3001 → |
transcriptional regulator, LysR‑family |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | AM260479 | 3,248,044 | 0 | C | . | 100.0%
| 57.7
/ NA
| 15 | coding (808/813 nt) | h16_A3001 | transcriptional regulator, LysR‑family |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base . (5/10); total (5/10) |
TCAAGACGTACGTGGAGATCGGGCTGGGCGTGGGATTCGTCGCCGGCGTCGCCTATGATGCCCGAGCCGCGACCCGCAACCTTGCCCGGCATCTCGGCCGGCCACCTGTTTGGCAGCAACCGTGACGCACCTGGCCGTC‑AGCAGGGTGCCTACCTGCGCAGCTTTGTGTACACCTTCATCGAGCTGTTCTCGCCGACGCTCAACCGCAAGCTGGTTGAGCAGGCCATGTCCGGCGAAC > AM260479/3247925‑3248162
|
tCAAGACGTACGTGGAGATCGGGCTGGGCGTGGGCATCGTCGCCGGCGTCGCCTATGATG‑CCGAG‑CGCGA‑CCGCAACC‑TGCGCGGCATCTCGGCCGGCCACCTGTTTGGCAGCAA‑CGTGACGCACCTGGCCGTc < 1:1093797/134‑1 (MQ=255)
ggCTGGGCGTGGGCATCGTCGCCGGCGTCGCCTATGATG‑CCGAG‑CGCGA‑CCGCAACC‑TGCGCGGCATCTCGGCCGGCCACCTGTTTGGCAGCAA‑CGTGACGCACCt < 1:692705/106‑1 (MQ=255)
ggCTGGGCGTGGGCATCGTCGCCGGCGTCGCCTATGATG‑CCGAG‑CGCGA‑CCGCAACC‑TGCGCGGCATCTCGGCCGGCCACCTGTTTGGCAGCAA‑CGTGACGCACCt > 2:692705/1‑106 (MQ=255)
ggCTGGGCGTGGGCATCGTCGCCGGCGTCGCCTATGATG‑CCGAG‑CGCGA‑CCGCAACC‑TGCGCGGCATCTCGGCCGGCCACCTGTTTGGCAGCAA‑CGTGACGCACCTGGCCGTCAAGCAGGGTGCCTACCTgcgc < 2:2694/134‑1 (MQ=255)
ggCTGGGCGTGGGCATCGTCGCCGGCGTCGCCTATGATG‑CCGAG‑CGCGA‑CCGCAACC‑TGCGCGGCATCTCGGCCGGCCACCTGTTTGGCAGCAA‑CGTGACGCACCTGGCCGTCAAGCAGGGTGCCTACCTgcg > 2:1125456/1‑133 (MQ=255)
ggCGTGGGCATCGTCGCCGGCGTCGCCTATGATG‑CCGAG‑CGCGA‑CCGCAACC‑TGCGCGGCATCTCGGCCGGCCACCTGTTTGGCAGCAA‑CGTGACGCACCTGGCCGTCAAGCAGGGTGCCTACCTGCGCAGCtt < 1:310863/134‑1 (MQ=255)
gTGGGCATCGTCGCCGGCGTCGCCTATGATG‑CCGAG‑CGCGA‑CCGCAACC‑TGCGCGGCATCTCGGCCGGCCACCTGTTTGGCAGCAA‑CGTGACGCACCTGGCCGTCAAGCAGGGTGCCTACCTgcgc < 2:253968/126‑1 (MQ=255)
ctGCGCGGCATCTCGGCCGGCCACCTGTTTGGCAGCAA‑CGTGACGCACCTGGCCGTCAAGCAGGGTGCCTACCTGCGCAGCTTTGTCTACACCTTCATCGAGCTGTTCTCGCCGACGCTCAACCGCAAGCTGGt < 2:184044/133‑1 (MQ=255)
cGGCATCTCGGCCGGCCACCTGTTTGGCAGCAA‑CGTGACGc > 1:291967/1‑41 (MQ=37)
cGGCATCTCGGCCGGCCACCTGTTTGGCAGCAA‑CGTGACGc < 2:291967/41‑1 (MQ=37)
gCATCTCGGCCGGCCACCTGTTTGGCAGCAA‑CGTGACGCACCTGGCCGTCAAGCAGGGTGCCTACCTGCGCAGCTTTGTCTACACCTTCATCGAGCTGTTCTCGCCGACGCTCAACCGCAAGCTGGTTGAGCAg > 2:990070/1‑134 (MQ=255)
aCCTGTTTGGCAGCAA‑CGTGACGCACCTGGCCGTCAAGCAGGGTGCCTACCTGCGCAGCTTTGTCTACACCTTCATCGAGCTGTTCTCGCCGACGCTCAACCGCAAGCTGGTTGAGCAGGCCATGTCCGGCGaa < 1:1125456/134‑1 (MQ=255)
ccTGTTTGGCAGCAA‑CGTGACGCACCTGGCCGTCAAGCAGGGTGCCTACCTGCGCAGCTTTGTCTACACCTTCATCGAGCTGTTCTCGCCGACGCTCAACCGCAAGCTGGTTGAGCAGGCCATGTCCGGCGAac < 1:990070/134‑1 (MQ=255)
ttGGCAGCAA‑CGTGACGCACCTGGCCGTCAAGCAGGGTGCCTAcc < 1:1096074/45‑1 (MQ=25)
ttGGCAGCAA‑CGTGACGCACCTGGCCGTCAAGCAGGGTGCCTAcc > 2:1096074/1‑45 (MQ=25)
|
TCAAGACGTACGTGGAGATCGGGCTGGGCGTGGGATTCGTCGCCGGCGTCGCCTATGATGCCCGAGCCGCGACCCGCAACCTTGCCCGGCATCTCGGCCGGCCACCTGTTTGGCAGCAACCGTGACGCACCTGGCCGTC‑AGCAGGGTGCCTACCTGCGCAGCTTTGTGTACACCTTCATCGAGCTGTTCTCGCCGACGCTCAACCGCAAGCTGGTTGAGCAGGCCATGTCCGGCGAAC > AM260479/3247925‑3248162
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
BRESEQ :: bam2aln output
GACGTGATCAAGACGTACGTGGAGATCGGGCTGGGCGTGGGATTCGTCGCCGGCGTCGCCTATGATGCCCGAGCCGCGACCCGCAACCTTGCCCGGCATCTCGGCCGGCCACCTGTTTGGCAGCAACCGTGACGCACCTGGCCGTC‑AGCAGGGTGCCTACCTGCGCAGCTTTGTGTACACCTTCATCGAGCTGTTCTCGCCGACGCTCAACCGCAAGCTGGTTGAGCAGGCCATGTCCGGCGAACACGAAGCCTACGAACTCTGAGGACGGCCCG > AM260479/3247918‑3248192
|
gtataagagacagCGTACGTGGAGATCGGGCTGGGCGTGGGCATCGTCGCCGGCGTCGCCTATGATG‑CCGAG‑CGCGA‑CCGCAACC‑TGCGCGGCATCTCGGCCGGCCACCTGTTTGGCAGCAA‑CGTGACGCACCTGGCCGTCAAGCAGGG < GWNJ‑0478:712:GW2002102894th:2:2106:13122:77887/136‑1 (MQ=60)
ATCAAGACGTACGTGGAGATCGGGCTGGGCGTGGGCATCGTCGCCGGCGTCGCCTATGATG‑CCGAG‑CGCGA‑CCGCAACC‑TGCGCGGCATCTCGGCCGGCCACCTGTTTGGCAGCAA‑CGTGACGCACCTGGCCGTCAAGCAGGGTGCCTAC < GWNJ‑0478:712:GW2002102894th:2:2213:4625:70100/150‑1 (MQ=60)
gagattagcgagtgtctcgtgggctcggagatgtgtataagagacagctatgatgccgagcgcgaccgcaacctgcgCGGCATCTCGGCCGGCCACCTGTTTGGCAGCAA‑CGTGACGCACCTGGCCGTCAAGCAGGGTGCCTACCTGCGC < GWNJ‑0478:712:GW2002102894th:2:1211:15374:9501/73‑1 (MQ=60)
GAGATCGGGCTGGGCGTGGGCATCGTCGCCGGCGTCGCCTATGATG‑CCGAG‑CGCGA‑CCGCAACC‑TGCGCGGCATCTCGGCCGGCCACCTGTTTGGCAGCAA‑CGTGACGCACCTGGCCGTCAAGCAGGGTGCCTACCTGCGCAGCTTTGTC > GWNJ‑0478:712:GW2002102894th:2:1114:10031:30035/1‑150 (MQ=60)
ttttttttcaagcagaagacggcatacgagattagcgagtgtctcgtgggctcggagatgtgtataagagacagCGGCCGGCCACCTGTTTGGCAGCAA‑CGTGACGCACCTGGCCGTCAAGCAGGGTGCCTACCTGCGCAGCTTTGTCT < GWNJ‑0478:712:GW2002102894th:2:2213:11749:81358/75‑1 (MQ=60)
GGGCGTGGGCATCGTCGCCGGCGTCGCCTATGATG‑CCGAG‑CGCGA‑CCGCAACC‑TGCGCGGCATCTCGGCCGGCCACCTGTTTGGCAGCAA‑CGTGACGCACCTGGCCGTCAAGCAGGGTGCCTACCTGCGCAGCTTTGTCTACACCTTCAT < GWNJ‑0478:712:GW2002102894th:2:1203:17456:52671/150‑1 (MQ=60)
agacggcatacgagattagcgagtgtctcgtgggctcggagatgtgtataagagacagGTTTGGCAGCAA‑CGTGACGCACCTGGCCGTCAAGCAGGGTGCCTACCTGCGCAGCTTTGTCTACACCTTCATCGAGCTGTTCTCGCCGACGC < GWNJ‑0478:712:GW2002102894th:2:2116:11606:67819/92‑1 (MQ=60)
CGA‑CCGCAACC‑TGCGCGGCATCTCGGCCGGCCACCTGTTTGGCAGCAA‑CGTGACGCACCTGGCCGTC‑Aagcctgtctcttatacacatctccgagcccacgagacactcgctaatctcgtatgccgtcttctgcttgaaaaaaaaaaa > GWNJ‑0478:712:GW2002102894th:2:1201:12998:34342/1‑68 (MQ=60)
CACCTGTTTGGCAGCAA‑CGTGACGCACCTGGCCGTCAAGCAGGGTGCCTACCTGCGCAGCTTTGTCTACACCTTCATCGAGCTGTTCTCGCCGACGCTCAACCGCAAGCTGGTTGAGCAGGCCATGTCCGGCGAACACGAAGCCTACGAA < GWNJ‑0478:712:GW2002102894th:2:2215:4903:23562/150‑1 (MQ=60)
ACCTGTTTGGCAGCAA‑CGTGACGCACCTGGCCGTCAAGCAGGGTGCCTACCTGCGCAGCTTTGTCTACACCTTCATCGAGCTGTTCTCGCCGACGCTCAACCGCAAGCTGGTTGAGCAGGCCATGTCCGGCGAACACGAAGCCTACGAAC < GWNJ‑0478:712:GW2002102894th:2:2207:20365:22187/150‑1 (MQ=60)
CAA‑CGTGACGCACCTGGCCGTCAAGCAGGGTGCCTACCTGCGCAGCTTTGTCTACACCTTCATCGAGCctgtctcttatacacatctccgagcccacgagacactcgctaatctcgtatgccgtcttctgcttgaaaaaaaaaaaaaca > GWNJ‑0478:712:GW2002102894th:2:2113:14270:70304/1‑68 (MQ=60)
ACGTGACGCACCTGGCCGTCAAGCAGGGTGCCTACCTGCGCAGCTTTGTCTACACCTTCATCGAGCTGTTCTCGCCGACGCTCAACCGCAAGCTGGTTGAGCAGGCCATGTCCGGCGAACACGAAGCCTACGAACTCTGAGGACtgtctc > GWNJ‑0478:712:GW2002102894th:2:1206:19708:72954/1‑144 (MQ=60)
|
GACGTGATCAAGACGTACGTGGAGATCGGGCTGGGCGTGGGATTCGTCGCCGGCGTCGCCTATGATGCCCGAGCCGCGACCCGCAACCTTGCCCGGCATCTCGGCCGGCCACCTGTTTGGCAGCAACCGTGACGCACCTGGCCGTC‑AGCAGGGTGCCTACCTGCGCAGCTTTGTGTACACCTTCATCGAGCTGTTCTCGCCGACGCTCAACCGCAAGCTGGTTGAGCAGGCCATGTCCGGCGAACACGAAGCCTACGAACTCTGAGGACGGCCCG > AM260479/3247918‑3248192
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
