Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A0 F0 I1 R1
|
146 |
27.9 |
2327082 |
89.7% |
2087392 |
107.8 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
AM260479 |
3,333,763 |
+G |
coding (5/615 nt) |
serB2 → |
phosphoserine phosphatase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | AM260479 | 3,333,760 | 1 | . | G | 100.0%
| 109.5
/ NA
| 29 | M1R (ATG→AGG) † | serB2 | phosphoserine phosphatase |
| Reads supporting (aligned to +/- strand): ref base . (0/0); new base G (17/12); total (17/12) |
CTGCCCGCCCGACCTCTCGCCTTAAGGTAAAATCGCCGCCCATGAATCTGGCACTCTTTGACCTCGACCACACCCTGATCCCGACCGACAGCGACCATGAAT‑GGGCCGCTTCCTGGTCCGCCTGGGCGTCGTCGACGAGGAAACCTACCGGCAGAAGAACGACGAGTTCTACGGCCACTACAAAGCCGGCACCCTCGATATCCAGGCCTTCCTGCGCTTTGCGCTGGCGC > AM260479/3333659‑3333888
|
ctgccCGCCCGACCTCTCGCCTTAAGGTAAAATCGCCGCCCATGAATCTGGCACTCTTTGACCTCGACCACACCCTGATCCCGACCGACAGCGACCATGAATGGGGCCGCTTCCTGGTCCGCCTGGGCgtcgtc < 1:1130438/134‑1 (MQ=255)
gccgccCATGAATCTGGCACTCTTTGACCTCGACCACACCCTGATCCCGACCGACAGCGACCATGAATGGGGCCGCTTCCTGGTCCGc > 2:371258/1‑88 (MQ=255)
gccgccCATGAATCTGGCACTCTTTGACCTCGACCACACCCTGATCCCGACCGACAGCGACCATGAATGGGGCCGCTTCCTGGTCCGc < 1:371258/88‑1 (MQ=255)
cccATGAATCTGGCACTCTTTGACCTCGACCACACCCTGATCCCGACCGACAGCGACCATGAATGGGGCCGCTTCCTGGTCCGCCTGGGCGTCGTCGACGAGGAAACCTACCGGCAGAAGAACGACGAGTTCTa < 2:808742/134‑1 (MQ=255)
ctTTGACCTCGACCACACCCTGATCCCGACCGACAGCGACCATGAATGGGGCCGCTTCCTGGTCCGCCTGGGCGTCGTCGACGAg < 2:921892/85‑1 (MQ=255)
ctTTGACCTCGACCACACCCTGATCCCGACCGACAGCGACCATGAATGGGGCCGCTTCCTGGTCCGCCTGGGCGTCGTCGACGAg > 1:921892/1‑85 (MQ=255)
aCCTCGACCACACCCTGATCCCGACCGACAGCGACCATGAATGGGGCCGCTTCCTGGTCCGCCTGGGCGTCGTCGACGAGGAAACCTACCGGCAGAAGAACGACGAGTTCTACGGCCACTACAAAGCCGGCAcc > 2:1068989/1‑134 (MQ=255)
cacaCCCTGATCCCGACCGACAGCGACCATGAATGGGGCCGCTTCCTGGTCCGCCTGGGCGTCGTCGACGAGGAAACCTACCGGCAGAAGAACGACGAGTTCTACGGCCACTACAAAGCCg > 1:536567/1‑121 (MQ=255)
cacaCCCTGATCCCGACCGACAGCGACCATGAATGGGGCCGCTTCCTGGTCCGCCTGGGCGTCGTCGACGAGGAAACCTACCGGCAGAAGAACGACGAGTTCTACGGCCACTACAAAGCCg < 2:536567/121‑1 (MQ=255)
cacaCCCTGATCCCGACCGACAGCGACCATGAATGGGGCCGCTTCCTGGTCCGCCTGGGCGTCGTCGACGAGGAAACCTACCGGCAGAAGAACGACGAGTTCTACGGCCACTACAAAGCCGGCAcc < 1:248717/126‑1 (MQ=255)
cacaCCCTGATCCCGACCGACAGCGACCATGAATGGGGCCGCTTCCTGGTCCGCCTGGGCGTCGTCGACGAGGAAACCTACCGGCAGAAGAACGACGAGTTCTACGGCCACTACAAAGCCGGCAcc > 2:248717/1‑126 (MQ=255)
cacaCCCTGATCCCGACCGACAGCGACCATGAATGGGGCCGCTTCCTGGTCCGCCTGGGCGTCGTCGACGAGGAAACCTACCGGCAGAAGAACGACGAGTTCTACGGCCACTACAAAGCCGGCACCCTCGatat > 1:53076/1‑134 (MQ=255)
acacCCTGATCCCGACCGACAGCGACCATGAATGGGGCCGCTTCCTGGTCCGCCTGGGCGTCGTCGACGAGGAAACCTACCGGCAGAAGAACGACGAGTTCTACGGCCACTACAAAGCCGGCACCCTCGATATc < 1:602564/134‑1 (MQ=255)
acacCCTGATCCCGACCGACAGCGACCATGAATGGGGCCGCTTCCTGGTCCGCCTGGGCGTCGTCGACGAGGAAACCTACCGGCAGAAGAACGACGAGTTCTACGGCCACTACAAAGCCGGCACCCTCGATATc > 1:715674/1‑134 (MQ=255)
cacCCTGATCCCGACCGACAGCGACCATGAATGGGGCCGCTTCCTGGTCCGCCTGGGCGTCGTCGACGAGGAAACCTACCGGCAGAAGAACGACGAGTTCTACGGCCACTACAAAGCCGGCACCCTCGATATc > 2:251811/1‑133 (MQ=255)
cccGACCGACAGCGACCATGAATGGGGCCGCTTCCTGGTCCGCCTGGGCGTCGTCGACGAGGAAACCTACCGGCAGAAGAACGACGAGTTCTACGGCCACTACAAAGCCGGCACCCTCGATATCCAGGCCTTcc > 2:448329/1‑134 (MQ=255)
cgaccgacAGCGACCATGAATGGGGCCGCTTCCTGGTCCGCCTGGGCGTCGTCGACGAGGAAACCTACCGGCAGAAGAACGACGAGTTCTACGGCCACTACAAAGCCg < 1:762092/108‑1 (MQ=255)
cgaccgacAGCGACCATGAATGGGGCCGCTTCCTGGTCCGCCTGGGCGTCGTCGACGAGGAAACCTACCGGCAGAAGAACGACGAGTTCTACGGCCACTACAAAGCCg > 2:762092/1‑108 (MQ=255)
cgaccgacAGCGACCATGAATGGGGCCGCTTCCTGGTCCGCCTGGGCGTCGTCGACGAGGAAACCTACCGGCAGAAGAACGACGAGTTCTACGGCCACTACAAAGCCGGCACCCTCGATATc < 2:196990/122‑1 (MQ=255)
cgaccgacAGCGACCATGAATGGGGCCGCTTCCTGGTCCGCCTGGGCGTCGTCGACGAGGAAACCTACCGGCAGAAGAACGACGAGTTCTACGGCCACTACAAAGCCGGCACCCTCGATATc > 1:196990/1‑122 (MQ=255)
cgacAGCGACCATGAATGGGGCCGCTTCCTGGTCCGCCTGGGCGTCGTCGACGAGGAAACCTACCGGCAGAAGAACGACGAGTTCTACGGCCACTACAAAGCCGGCACCCTCGATATCCAGGCCTTCCTGCGCt > 1:107112/1‑134 (MQ=255)
acAGCGACCATGAATGGGGCCGCTTCCTGGTCCGCCTGGGCGTCGTCGACGAGGAAACCTACCGGCAGAAGAACGACGAGTTCTACGGCCACTACAAAGCCGGCAcc > 2:207270/1‑107 (MQ=255)
acAGCGACCATGAATGGGGCCGCTTCCTGGTCCGCCTGGGCGTCGTCGACGAGGAAACCTACCGGCAGAAGAACGACGAGTTCTACGGCCACTACAAAGCCGGCAcc < 1:207270/107‑1 (MQ=255)
acAGCGACCATGAATGGGGCCGCTTCCTGGTCCGCCTGGGCGTCGTCGACGAGGAAACCTACCGGCAGAAGAACGACGAGTTCTACGGCCACTACAAAGCCGGCACCCTCGATATCCAGGCCTTCCTGCGCttt > 2:712527/1‑134 (MQ=255)
acAGCGACCATGAATGGGGCCGCTTCCTGGTCCGCCTGGGCGTCGTCGACGAGGAAACCTACCGGCAGAAGAACGACGAGTTCTACGGCCACTACAAAGCCGGCACCCTCGATATCCAGGCCTTCCTGCGCttt > 1:131214/1‑134 (MQ=255)
acAGCGACCATGAATGGGGCCGCTTCCTGGTCCGCCTGGGCGTCGTCGACGAGGAAACCTACCGGCAGAAGAACGACGAGTTCTACGGCCACTACAAAGCCGGCACCCTCGATATCCAGGCCTTCCTGCGCttt > 1:450393/1‑134 (MQ=255)
gCGACCATGAATGGGGCCGCTTCCTGGTCCGCCTGGGCGTCGTCGACGAGGAAACCTACCGGCAGAAGAACGACGAGTTCTACGGCCACTACAAAGCCGGCACCCTCGATATCCAGGCCTTCCTGCGCTTTgcg < 2:450393/134‑1 (MQ=255)
tGAATGGGGCCGCTTCCTGGTCCGCCTGGGCGTCGTCGACGAGGAAACCTACCGGCAGAAGAACGACGAGTTCTACGGCCACTACAAAGCCGGCACCCTCGATATCCAGGCCTTCCTGCGCTTTGCGCTGgcgc < 2:53076/134‑1 (MQ=255)
tGAATGGGGCCGCTTCCTGGTCCGCCTGGGCGTCGTCGACGAGGAAACCTACCGGCAGAAGAACGACGAGTTCTACGGCCACTACAAAGCCGGCACCCTCGATATCCAGGCCTTCCTGCGCTTTGCGCTGgcgc > 2:153815/1‑134 (MQ=255)
|
CTGCCCGCCCGACCTCTCGCCTTAAGGTAAAATCGCCGCCCATGAATCTGGCACTCTTTGACCTCGACCACACCCTGATCCCGACCGACAGCGACCATGAAT‑GGGCCGCTTCCTGGTCCGCCTGGGCGTCGTCGACGAGGAAACCTACCGGCAGAAGAACGACGAGTTCTACGGCCACTACAAAGCCGGCACCCTCGATATCCAGGCCTTCCTGCGCTTTGCGCTGGCGC > AM260479/3333659‑3333888
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
BRESEQ :: bam2aln output
CGCCTGCCTGCCTGCCCGCCCGACCTCTCGCCTTAAGGTAAAATCGCCGCCCATGAATCTGGCACTCTTTGACCTCGACCACACCCTGATCCCGACCGACAGCGACCATGAAT‑GGGCCGCTTCCTGGTCCGCCTGGGCGTCGTCGACGAGGAAACCTACCGGCAGAAGAACGACGAGTTCTACGGCCACTACAAAGCCGGCACCCTCGATATCCAGGCCTTCCTGCGCTTTGCGCTGGCGCCG > AM260479/3333648‑3333890
|
cgtgggctcggagatgtgtataagagacaGCCTTAAGGTAAAATCGCCGCCCATGAATCTGGCACTCTTTGACCTCGACCACACCCTGATCCCGACCGACAGCGACCATGAATGGGGCCGCTTCCTGGTCCGCCTGGGCGTCGTCGAC < GWNJ‑0478:712:GW2002102894th:2:1207:3243:17896/119‑1 (MQ=60)
CCTGCCCGCCCGACCTCTCGCCTTAAGGTAAAATCGCCGCCCATGAATCTGGCACTCTTTGACCTCGACCACACCCTGATCCCGACCGACAGCGACCATGAATGGGGCCGCTTCCTGGTCCGCCTGGGCGTCGTCGACGAGGAAACCTAC < GWNJ‑0478:712:GW2002102894th:2:2215:14863:46390/150‑1 (MQ=60)
ttttcaagcagaagacggcatacgagattagcgagtgtctcgtgggctcggagatgtgtataagagacagCCGACAGCGACCATGAATGGGGCCGCTTCCTGGTCCGCCTGGGCGTCGTCGACGAGGAAACCTACCGGCAGAAGAACGA < GWNJ‑0478:712:GW2002102894th:2:2112:5805:68366/79‑1 (MQ=60)
agacggcatacgagattagcgagtgtctcgtgggctcggagatgtgtataagagacagcatgaatgGGGCCGCTTCCTGGTCCGCCTGGGCGTCGTCGACGAGGAAACCTACCGGCAGAAGAACGACGAGTTCTACGGCCACTACAAAGC < GWNJ‑0478:712:GW2002102894th:2:2206:14687:44012/84‑1 (MQ=60)
CATGAATCTGGCACTCTTTGACCTCGACCACACCCTGATCCCGACCGACAGCGACCATGAATGGGGCCGCTTCCTGGTCCGCCTGGGCGTCGTCGACGAGGAAACCTACCGGCAGctgtctcttatacacatctccgagcccacgagac > GWNJ‑0478:712:GW2002102894th:2:2204:20605:26275/1‑115 (MQ=60)
CTGGCACTCTTTGACCTCGACCACACCCTGATCCCGACCGACAGCGACCATGAAT‑GGGgccgcttctgtctcttatacacatctccgagcccacgagacactcgctaatctcgtatgccgtcttctgcttgaaaaaaaaaaaaaaacaa > GWNJ‑0478:712:GW2002102894th:2:2116:15765:31414/1‑58 (MQ=60)
gcgagtgtctcgtgggctcggagatgtgtataagagacaGACCATGAATGGGGCCGCTTCCTGGTCCGCCTGGGCGTCGTCGACGAGGAAACCTACCGGCAGAAGAACGACGAGTTCTACGGCCACTACAAAGCCGGCACCCTCGATATC < GWNJ‑0478:712:GW2002102894th:2:2212:19250:69528/111‑1 (MQ=60)
CTCTTTGACCTCGACCACACCCTGATCCCGACCGACAGCGACCATGAATGGGGCCGCTTCCTGGTCCGCCTGGGCGTCGTCGACGAGGAAACCTACCGGCAGAAGAACGACGAGTTCTACGGCCACTACAAAGCCGGCACCCTCGATATC > GWNJ‑0478:712:GW2002102894th:2:1102:3823:30082/1‑150 (MQ=60)
CTCTTTGACCTCGACCACACCCTGATCCCGACCGACAGCGACCATGAATGGGGCCGCTTCCTGGTCCGCCTGGGCGTCGTCGACGAGGAAACCTACCGGCAGAAGAACGACGAGTTCTACGGCCACTACAAAGCCGGCACCCTCGATATC > GWNJ‑0478:712:GW2002102894th:2:1215:9286:78155/1‑150 (MQ=60)
attagcgagtgtctcgtgggctcggagatgtgtataagagacaggaatgGGGCCGCTTCCTGGTCCGCCTGGGCGTCGTCGACGAGGAAACCTACCGGCAGAAGAACGACGAGTTCTACGGCCACTACAAAGCCGGCACCCTCGATATCC < GWNJ‑0478:712:GW2002102894th:2:1214:1729:54365/101‑1 (MQ=60)
TCTTTGACCTCGACCACACCCTGATCCCGACCGACAGCGACCATGAATGGGGCCGCTTCCTGGTCCGCCTGGGCGTCGTCGACGAGGAAACCTACCGGCAGAAGAACGACGAGTTCTACGGCCACTACAAAGCCGGCACCCTCGATATCC > GWNJ‑0478:712:GW2002102894th:2:2109:6293:6870/1‑150 (MQ=60)
ataagagacaGACCACACCCTGATCCCGACCGACAGCGACCATGAATGGGGCCGCTTCCTGGTCCGCCTGGGCGTCGTCGACGAGGAAACCTACCGGCAGAAGAACGACGAGTTCTACGGCCACTACAAAGCCGGCACCCTCGATATCC < GWNJ‑0478:712:GW2002102894th:2:2110:15774:56059/139‑1 (MQ=60)
gtataagagacagCCCTGATCCCGACCGACAGCGACCATGAATGGGGCCGCTTCCTGGTCCGCCTGGGCGTCGTCGACGAGGAAACCTACCGGCAGAAGAACGACGAGTTCTACGGCCACTACAAAGCCGGCACCCTCGATATCCAGGCC < GWNJ‑0478:712:GW2002102894th:2:1113:17677:94538/137‑1 (MQ=60)
GACCTCGACCACACCCTGATCCCGACCGACAGCGACCATGAATGGGGCCGCTTCCTGGTCCGCCTGGGCGTCGTCGACGAGGAAACCTACCGGCAGAAGAACGACGAGTTCTACGGCCACTACAAAGCCGGCACCCTCGATATCCAGGCC < GWNJ‑0478:712:GW2002102894th:2:1114:10428:3785/150‑1 (MQ=60)
ACCACACCCTGATCCCGACCGACAGCGACCATGAATGGGGCCGCTTCCTGGTCCGCCTGGGCGTCGTCGACGAGGAAACCTACCGGCAGAAGAACGACGAGTTCTACGGCCACTACAAAGCCGGCACCCTCGATATCCAGGCCTTCCTGC > GWNJ‑0478:712:GW2002102894th:2:1113:15310:42315/1‑150 (MQ=60)
CACACCCTGATCCCGACCGACAGCGACCATGAATGGGGCCGCTTCCTGGTCCGCCTGGGCGTCGTCGACGAGGAAACCTACCGGCAGAAGAACGACGAGTTCTACGGCCACTACAAAGCCGGCACCCTCGATATCCAGGCCTTCCTGCGC < GWNJ‑0478:712:GW2002102894th:2:2103:3948:25975/150‑1 (MQ=60)
CACCCTGATCCCGACCGACAGCGACCATGAATGGGGCCGCTTCCTGGTCCGCCTGGGCGTCGTCGACGAGGAAACCTACCGGCAGAAGAACGACGAGTTCTACGGCCACTACAAAGCCGGCACCCTCGATATCCAGGCCTTCCTGCGCTT > GWNJ‑0478:712:GW2002102894th:2:1106:14359:53165/1‑150 (MQ=60)
CCCTGATCCCGACCGACAGCGACCATGAATGGGGCCGCTTCCTGGTCCGCCTGGGCGTCGTCGACGAGGAAACCTACCGGCAGAAGAACGACGAGTTCTACGGCCACTACAAAGCCGGCACCCTCGATATCCAGGCCTTCCTGCGCTTTG > GWNJ‑0478:712:GW2002102894th:2:1211:16496:53380/1‑150 (MQ=60)
CCCTGATCCCGACCGACAGCGACCATGAATGGGGCCGCTTCCTGGTCCGCCTGGGCGTCGTCGACGAGGAAACCTACCGGCAGAAGAACGACGAGTTCTACGGCCACTACAAAGCCGGCACCCTCGATATCCAGGCCTTCCTGCGCTTTG > GWNJ‑0478:712:GW2002102894th:2:1108:4388:89705/1‑150 (MQ=60)
ACCGACAGCGACCATGAATGGGGCCGCTTCCTGGTCCGCCTGGGCGTCGTCGACGAGGAAACCTACCGGCAGAAGAACGACGAGTTCTACGGCCACTACAAAGCCGGCACCCTCGATATCCAGGCCTTCCTGCGCTTTGCGCTGGCGCCG > GWNJ‑0478:712:GW2002102894th:2:1216:20136:88560/1‑150 (MQ=60)
|
CGCCTGCCTGCCTGCCCGCCCGACCTCTCGCCTTAAGGTAAAATCGCCGCCCATGAATCTGGCACTCTTTGACCTCGACCACACCCTGATCCCGACCGACAGCGACCATGAAT‑GGGCCGCTTCCTGGTCCGCCTGGGCGTCGTCGACGAGGAAACCTACCGGCAGAAGAACGACGAGTTCTACGGCCACTACAAAGCCGGCACCCTCGATATCCAGGCCTTCCTGCGCTTTGCGCTGGCGCCG > AM260479/3333648‑3333890
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
