Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A0 F0 I1 R1
|
146 |
27.9 |
2327082 |
89.7% |
2087392 |
107.8 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
AM260480 |
2,431,093 |
Δ1 bp |
coding (2380/2469 nt) |
h16_B2148 → |
membrane carboxypeptidase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | AM260480 | 2,431,092 | 0 | T | . | 100.0%
| 57.2
/ NA
| 14 | coding (2379/2469 nt) | h16_B2148 | membrane carboxypeptidase |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base . (6/8); total (6/8) |
GCCCGGCACCGCGTCGGCCGGCGCTGCCCACGCCGGAGGCCGAGGTCGCGCCGCCGGCCGGCGCGGCATCGTCTGCCGCGGTGGATGTCGGCCCGGATGAGTCCAGCCTGGCGCTGGATTCGGGGGGAGACGGTGATTGCGGTGCCGCAGGACATGAGCGCGGGCAGCGCTCCGGTTGCGCCACCCCCTCAGGCCGA > AM260480/2430974‑2431170
|
cgtgggCACCGCGTCGGCCGGCGCTGCCCACGCCGGAGGCCGAGGTCGCGCCGCCGGCCGGCGCGGCATCGTCTGCCGCGGTGGATGTCGGCCCGGATGAGTCCAGCCTGGCGCTGGA‑TCGGGGGGAGACGGt > 1:1093365/5‑133 (MQ=255)
cGGCACCGCGTCGGCCGGCGCTGCCCACGCCGGAGGCCGAGGTCGCGCCGCCGGCCGGCGCGGCATCGTCTGCCGCGGTGGATGTCGGCCCGGATGAGTCCAGCCTGGCGCTGGA‑TCGGGGGGAGACGGTGAtt > 2:1099570/1‑134 (MQ=255)
ggCACCGCGTCGGCCGGCGCTGCCCACGCCGGAGGCCGAGGTCGCGCCGCCGGCCGGCGCGGCATCGTCTGCCGCGGTGGATGTCGGCCCGGATGAGTCCAGCCTGGCGCTGGA‑TCGGGGGGAGACGGTGATTg < 1:373135/134‑1 (MQ=255)
ggCACCGCGTCGGCCGGCGCTGCCCACGCCGGAGGCCGAGGTCGCGCCGCCGGCCGGCGCGGCATCGTCTGCCGCGGTGGATGTCGGCCCGGATGAGTCCAGCCTGGCGCTGGA‑TCGGGGGGAGACGGTGATTg < 1:980202/134‑1 (MQ=255)
gcgcTGCCCACGCCGGAGGCCGAGGTCGCGCCGCCGGCCGGCGCGGCATCGTCTGCCGCGGTGGATGTCGGCCCGGATGAGTCCAGCCTGGCGCTGGA‑TCGGGGGGAGACGGTGATTGCGGTGCCGCAGGACAt > 2:589278/1‑134 (MQ=255)
aGGCCGAGGTCGCGCCGCCGGCCGGCGCGGCATCGTCTGCCGCGGTGGATGTCGGCCCGGATGAGTCCAGCCTGGCGCTGGA‑TCGGGGGGAGACGGTGATTGCGGTGCCGCAGGACATGAGCGCGGGCAGCGCt < 1:384824/134‑1 (MQ=255)
tcggccgg‑cggCGCGGCATCGTCTGCCGCGGTGGATGTCGGCCCGGATGAGTCCAGCCTGGCGCTGGA‑TCGGGGGGAGACGGTGATTGCGGTGCCGCAGGACATGAGCGCGGGCAGCGCTCCGGTTGCGCCAcc > 1:628035/4‑134 (MQ=255)
gcgcGGCATCGTCTGCCGCGGTGGATGTCGGCCCGGATGAGTCCAGCCTGGCGCTGGA‑TCGGGGGGAGACGGTGATTGCGGTGCCGCAGGACATGAGCGCGGGCAGCGCTCCGGTTGCGCCACCCCCTCAGGcc < 1:589278/134‑1 (MQ=255)
gcgcGGCATCGTCTGCCGCGGTGGATGTCGGCCCGGATGAGTCCAGCCTGGCGCTGGA‑TCGGGGGGAGACGGTGATTGCGGTGCCGCAGGACATGAGCGCGGGCAGCGCTCCGGTTGCGCCACCCCCTCAGGcc < 2:1093365/134‑1 (MQ=255)
gcgGCATCGTCTGCCGCGGTGGATGTCGGCCCGGATGAGTCCAGCCTGGCGCTGGA‑TCGGGGGGAGACGGTGATTGCGGTGCCGCAGGACATGAGCGCGGGCAGCGCTCCGGTTGCGCCACCCCCTCAGGCCGa < 2:628035/134‑1 (MQ=255)
gATGAGTCCAGCCTGGCGCTGGA‑TCGGGGGGAGACGGTGATTg > 1:776755/1‑43 (MQ=38)
gATGAGTCCAGCCTGGCGCTGGA‑TCGGGGGGAGACGGTGATTg < 2:776755/43‑1 (MQ=38)
gATGAGTCCAGCCTGGCGCTGGA‑TCGGGGGGAGACGGTGATTGCGGTGCCGCAGGACATGAGCGCGGGCAGCGCTCCGGTTGCGCCAcc < 1:1047599/89‑1 (MQ=255)
gATGAGTCCAGCCTGGCGCTGGA‑TCGGGGGGAGACGGTGATTGCGGTGCCGCAGGACATGAGCGCGGGCAGCGCTCCGGTTGCGCCAcc > 2:1047599/1‑89 (MQ=255)
|
GCCCGGCACCGCGTCGGCCGGCGCTGCCCACGCCGGAGGCCGAGGTCGCGCCGCCGGCCGGCGCGGCATCGTCTGCCGCGGTGGATGTCGGCCCGGATGAGTCCAGCCTGGCGCTGGATTCGGGGGGAGACGGTGATTGCGGTGCCGCAGGACATGAGCGCGGGCAGCGCTCCGGTTGCGCCACCCCCTCAGGCCGA > AM260480/2430974‑2431170
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
BRESEQ :: bam2aln output
AGACCCCGGCGCCGCGCCCGGCACCGCGTCGGCCGGCGCTGCCCACGCCGGAGGCCGAGGTCGCGCCGCCGGCCGGCGCGGCATCGTCTGCCGCGGTGGATGTCGGCCCGGATGAGTCCAGCCTGGCGCTGGATTCGGGGGGAGACGGTGATTGCGGTGCCGCAGGACATGAGCGCGGGCAGCGCTCCGGTTGCGCCACCCCCTCAGGCCGAACCAGCGCCTAACGCCGCCCCTGCCTCCCCGGCTTCCGCAAATCCGCCCGCACCGCCTGCGCCCCCAGCGC > AM260480/2430959‑2431241
|
gacctcggcctccggcgtgGGCACCGCGTCGGCCGGCGCTGCCCACGCCGGAGGCCGAGGTCGCGCCGCCGGCCGGCGCGGCATCGTCTGCCGCGGTGGATGTCGGCCCGGATGAGTCCAGCCTGGCGCTGGA‑TCGGGGGGAGACGGTG > GWNJ‑0478:712:GW2002102894th:2:2213:17294:67957/20‑149 (MQ=60)
CCCCGGCGCCGCGCCCGGCACCGCGTCGGCCGGCGCTGCCCACGCCGGAGGCCGAGGTCGCGCCGCCGGCCGGCGCGGCATCGTCTGCCGCGGTGGATGTCGGCCCGGATGAGTCCAGCCTGGCGCTGGA‑TCGGGGGGAGACctgtctct > GWNJ‑0478:712:GW2002102894th:2:2104:13228:44595/1‑142 (MQ=60)
CGGCACCGCGTCGGCCGGCGCTGCCCACGCCGGAGGCCGAGGTCGCGCCGCCGGCCGGCGCGGCATCGTCTGCCGCGGTGGATGTCGGCCCGGATGAGTCCAGCCTGGCGCTGGA‑TCGGGGGGAGACGGTGATTGCGGTGCCGCAGGACA < GWNJ‑0478:712:GW2002102894th:2:1207:7429:27894/150‑1 (MQ=60)
CGGCACCGCGTCGGCCGGCGCTGCCCACGCCGGAGGCCGAGGTCGCGCCGCCGGCCGGCGCGGCATCGTCTGCCGCGGTGGATGTCGGCCCGGATGAGTCCAGCCTGGCGCTGGA‑TCGGGGGGAGACGGTGATTGCGGTGCCGCAGGACA < GWNJ‑0478:712:GW2002102894th:2:2206:21183:64343/150‑1 (MQ=60)
gcgcccggcaccgcgtcggccggCGGCGCGGCATCGTCTGCCGCGGTGGATGTCGGCCCGGATGAGTCCAGCCTGGCGCTGGA‑TCGGGGGGAGACGGTGATTGCGGTGCCGCAGGACATGAGCGCGGGCAGCGCTCCGGTTGCGCCACCC > GWNJ‑0478:712:GW2002102894th:2:2102:17413:89383/24‑150 (MQ=60)
GAGGCCGAGGTCGCGCCGCCGGCCGGCGCGGCATCGTCTGCCGCGGTGGATGTCGGCCCGGATGAGTCCAGCCTGGCGCTGGA‑TCGGGGGGAGACGGTGATTGCGGTGCCGCAGGACATGAGCGCGGGCAGCGCTCCGGTTGCGCCACCC < GWNJ‑0478:712:GW2002102894th:2:1207:13389:93209/150‑1 (MQ=60)
cgtgggctcggagatgtgtataagagacaGGTGGATGTCGGCCCGGATGAGTCCAGCCTGGCGCTGGA‑TCGGGGGGAGACGGTGATTGCGGTGCCGCAGGACATGAGCGCGGGCAGCGCTCCGGTTGCGCCACCCCCTCAGGCCGAACC < GWNJ‑0478:712:GW2002102894th:2:2210:7169:33885/120‑1 (MQ=60)
GGCGCGGCATCGTCTGCCGCGGTGGATGTCGGCCCGGATGAGTCCAGCCTGGCGCTGGA‑TCGGGGGGAGACGGTGATTGCGGTGCCGCAGGACATGAGCGCGGGCAGCGCTCCGGTTGCGCCACCCCCTCAGGCCGAACCAGCGCCTAAC < GWNJ‑0478:712:GW2002102894th:2:2101:5362:41371/150‑1 (MQ=60)
GTGGATGTCGGCCCGGATGAGTCCAGCCTGGCGCTGGA‑TCGGGGGGAGACGGTGATTGCGGTGCCGCAGGACActgtctcttatacacatctccgagcccacgagacactcgctaatctcgtatgccgtcttctgcttgaaaaaaaaaa > GWNJ‑0478:712:GW2002102894th:2:2113:21142:33440/1‑73 (MQ=60)
GCGCTGGA‑TCGGGGGGAGACGGTGATTGCGGTGCCGCAGGACATGAGCGCGGGCAGCGCTCCGGTTGCGCCACCCCCTCAGGCCtgtctcttatacacatctccgagcccacgagacactcgctaatctcgtatgccgtcttctgcttg > GWNJ‑0478:712:GW2002102894th:2:2105:14117:49621/1‑84 (MQ=60)
gaTCGGGGGGAGACGGTGATTGCGGTGCCGCAGGACATGAGCGCGGGCAGCGCTCCGGTTGCGCCACCCCCTCAGGCCGAACCAGCGCCTAACGCCGCCCCTGCCTCCCCGGCTTCCGCAAATCCGCCCGCACCGCCTGCGCCCCCAGCG > GWNJ‑0478:712:GW2002102894th:2:1108:15860:30497/3‑150 (MQ=60)
ATCGGGGGGAGACGGTGATTGCGGTGCCGCAGGACATGAGCGCGGGCAGCGCTCCGGTTGCGCCACCCCCTgtctcttatacacatctccgagcccacgagacactcgctaatctcgtatgccgtcttctgcttgaaaaaaaaaaaaaa > GWNJ‑0478:712:GW2002102894th:2:1113:16826:10674/1‑71 (MQ=60)
ATCGGGGGGAGACGGTGATTGCGGTGCCGCAGGACATGAGCGCGGGCAGCGCTCCGGTTGCGCCACCCCCTCAGGCCGAACCAGCGCCTAACGCCGCCCCTGCCTCCCCGGCTTCCGCAAATCCGCCCGCACCGCCTGCGCCCCCAGCGC > GWNJ‑0478:712:GW2002102894th:2:2113:4338:57803/1‑150 (MQ=60)
|
AGACCCCGGCGCCGCGCCCGGCACCGCGTCGGCCGGCGCTGCCCACGCCGGAGGCCGAGGTCGCGCCGCCGGCCGGCGCGGCATCGTCTGCCGCGGTGGATGTCGGCCCGGATGAGTCCAGCCTGGCGCTGGATTCGGGGGGAGACGGTGATTGCGGTGCCGCAGGACATGAGCGCGGGCAGCGCTCCGGTTGCGCCACCCCCTCAGGCCGAACCAGCGCCTAACGCCGCCCCTGCCTCCCCGGCTTCCGCAAATCCGCCCGCACCGCCTGCGCCCCCAGCGC > AM260480/2430959‑2431241
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
