Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 6081 0.0 0 0.0% 0 0.0

Breseq alignment

N/A

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TCCGGCGAAAAAATCGTAAGAAACGAAAGGAGAACGAGCCCACAGGCGGGGTAACATCAAAAGGATTTATATTAGATCGTAAAGTCAATGCATATGTAACAGTACAGAGGTAGAAGGTATGTATGTGTAACGATTAAGAGATGCAATCATGATTGTTCTTCGTCAGACGAATCTTCGAATATGTCTTCGTCAATGACACAGTCGTTCTCCCAAAAGTCATGGGCGAGCTTGCTTAAAACGGCGGCAGCGTTTTGACGCTGCGCTCTACTTGAATCTTTCTCATTAATGTACTCGTTATACTCTTTAAAAGTTGATCCACTGAGAGATTGTGACTGCGATGTTCTACGTGGAGTATAGTAACTAGCAGGATTGAGCCTCCTGCACTCTTTGAGCCTCAATAGAGAAGCGATCTCCTCGGAGAGTTTTCTTCCCTTATGCTGGTGACCATCTTTAAGTTGTTTGTGTTGTCCTTGTGAAACTGCTGTTTCCATTTTGCCGCGTGGAGACATCTGTCCAATTTGCCCCTGGCTCTCTGTAAATACCCATATATGAACGTTATATATATTGTATATTAATG  >  BK006934/374508‑375084
                                                                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                                                                                    
TCCGACGAAAAAATCGTAAGAAACGAAAGGAGAACGAGCCCACAGGCGGGGTAACATCAAAAGGATTTATATTAGATCGTAAAGTCAATGCATATGTAACAGTACAGAGGTAGAAGGTATGTATGTGTAACGATTAAGAGATGCAATCATGATTGTTCTTCGTCAGACGAATCTTCGAATATGTCTTCGTCAATGACACAGTCGTTCTCCCAAAAGTCATGGGCGAGCTTGCTTAAAACGGCGGCAGCGTTTTGACGCTGCGCCCTGCTTGAATCTTTCTCATTGACGTACTCGTTATACT                                                                                                                                                                                                                                                                                      >  SRR13385771.1294651/1‑301 (MQ=60)
                 AAGAAACGAAAGGAGAACGAGCCCACAGGCGGGGTAACATCAAAAGGATTTATATTAGATCGTAAAGTCAATGCATATGTAACAGTACAGAGGTAGAAGGTATGTATGTGTAACGATTAAGAGATGCAATCATGATTGTTCTTCGTCAGACGAATCTTCGAATATGTCTTCGTCAATGCCACAGTCGTTCTCCCACAAGTCATGGGCGAGCTTGCTTAACACGGCGGCAGCGTTTTGACGCTGCGCTCTGCTTGAATCTTTCTCATTGACGTACTCGTTATACTCTTTAAAAGTTGATCC                                                                                                                                                                                                                                                                      >  SRR13385771.934954/1‑300 (MQ=60)
                                                         CAAAAGGATTTATATTAGATCGTAAAGTCAATGCATATGTAACAGTACAGAGGTAGAAGGTATGTATGTGTAACGATTAAGAGATGCAATCATGATTGTTCTTCGTCAGACGAATCTTCGAATATGTCTTCGTCAATGACACAGTCGTTCTCCCAAAAGTCATGGGCGAGCTTGCTTAAAACGGCGGCAGCGTTTTGACGCTGCGCTCTGCTTGAATCTTTCTCATTGACGTACTCGTTATACCCTTTAAAAGTTGATCCACTGAGAGATTGTGACTGCGATGTTCTACGTGGAGTATAGT                                                                                                                                                                                                                             >  SRR13385771.106250/1‑301 (MQ=60)
                                                                  TTATATTAGATCGTAAAGTCAATGCATATGTAACAGTACAGAGGTAGAAGGTATGTATGTGTAACGATTAAGAGATGCAATCATGATTGTTCTTCGTCAGACGAATCTTCGAATATGTCTTCGTCAATGACACAGTCGTTCTCCCAAAAGTCATGGGCGAGCTTGCTTAAAACGGCGGCAGCGTTTTGACGCTGCGCTCTGCTTGAATCTTTCTCATTGACGTACTCGTTATACTCTTTAAAAGTTGATCCACTGAGAGATTGTGACTGCGATGTTCTACGTGGAGTATAGTAACTAGCA                                                                                                                                                                                                                     <  SRR13385771.1213326/300‑1 (MQ=60)
                                                                     TATTAGATCGTAAAGTCAATGCATATGTAACAGTACATAGGTAGAAGGTATTTATGGGTAACGATTAAGAGATGCAATCATTATTGTTCTTCGTCAGGCGAATCTTCGAATATGTCTTCTTCAATGACACAGTCGTTCTCCCAAAAGTCATGGGCGAGCTTGCTGAAAACGGCGGCAGCGTTTTGACGCTGCTCTCTGCTTGAAGCTTTCTCATGGACGTACTCGTTATACTCTTTAAAAGTTGATCCACGGAGAGATTGTGACTGCGATGTTCGACGTGGAGTATAGTAACTAGCAGGA                                                                                                                                                                                                                  <  SRR13385771.1872575/300‑1 (MQ=60)
                                                                             CGTAAAGTCAATGCATATGTAACAGTACAGAGGTAGAAGGTATGTATGTGTAACGATTAAGAGATGCAATCATGATTGTTCTTCGTCAGACGAATCTTCGAATATGTCTTCGTCAATGACACAGTCGTTCTCCCAAAAGTCATGGGCGAGCTTGCTTAAAACGGCGGCAGCGTTTTGACGCTGCGCTCTGCTTGAATCTTTCTCATTGACGTACTCGTTATACTCTTTAAAAGTTGATCCACTGAGAGATTGTGACTGCGATGTTCTACGTGGAGTATAGTAACTAGCAGGATTGAGCCT                                                                                                                                                                                                          <  SRR13385771.1509691/300‑1 (MQ=60)
                                                                                          CATATGTAACAGTACAGAGGTAGAAGGTATGTATGTGTAACGATTAAGAGATGCAATCATGATTGTTCTTCGTCAGACGAATCTTCGAATATGTCTTCGTCAATGACACAGTCGTTCTCCCAAAAGTCATGGGCGAGCTTGCTTAAAACGGCGGCAGCGTTTTGACGCTGCGCTCTGCTTGAATCTTTCTCATTGACGTACTCGTTATACTCTTTAAAAGTTGATCCACTGAGAGATTGTGACTGCGATGTTCTACGTGGAGTATAGTAACTAGCAGGATTGAGCCTCCTGCACTCTTTG                                                                                                                                                                                             >  SRR13385771.888477/1‑300 (MQ=60)
                                                                                                  ACAGTACAGAGGTAGAAGGTATGTATGTGTAACGATTAAGAGATGCAATCATGATTGTTCTTCGTCAGACGAATCTTCGAATATGTCTTCGTCAATGACACAGTCGTTCTCCCAAAAGTCATGGGCGAGCTTGCTTAAAACGGCGGCAGCGTTTTGACGCTGCGCTCTGCTTGAATCTTTCTCATTGACGTACTCGTTATACTCTTTAAAAGTTGATCCACTGAGAGATTGTGACTGCGATGTTCTACGTGGAGTATAGTAACTAGCAGGATTGAGCCTCCTGCACTCTTTGAGCCTCAAT                                                                                                                                                                                    <  SRR13385771.760705/301‑1 (MQ=60)
                                                                                                   CAGTACAGAGGTAGAAGGTATGTATGTGTAACGATTAAGAGATGCAATCATGATTGTTCTTAGTCAGACGAATCTTCGAATATGTCTTCGTCAATGACACAGTCGTTCTCCCAAAAGTCATGGGCGAGCTTGCTTAAAACGGCGGCAGCGTTTTGACGCTGCGCTCTGCTTGAATCTTTCTCATTGACGTACTCGTTATACTCTTTAAAAGTTGATCCACTGAGAGATTGTGACTGCGATGTTCTACGTGGAGTATAGTAACTAGCAGGATTGAGCCTCCTGCACTCTTTGAGCATCAAT                                                                                                                                                                                    >  SRR13385771.1214004/1‑300 (MQ=60)
                                                                                                    AGTACAGAGGTAGAAGGCATGTATGTGTAACGATTAAGAGATGCACTCATGATTGTTCTTCGTCAGACGAATCTTCGAATATGTCTTCGTCAATGACACAGTCGTTCTCCCAAACGTCATGGGCGAGCTTGCTTAAAACGGCGGCAGCGTTTTGACGCTGCGCCCTGCTTGACTCTTTCTCATTGACGTACTCGTTATACTCTCTAAAAGTTGATCCACTGAGAGATTGTGACTGCGATGTTCTACGTGGAGTATAGTAACTAGCAGGATTGAGCCTCCTGCCCTCTTTGAGCCTCAAT                                                                                                                                                                                    >  SRR13385771.7377/1‑299 (MQ=60)
                                                                                                                                   GATTAAGAGATGCAATCATGATTGTTCTTCGTCAGACGAATCTTCGAATATGTCTTCGTCAATGACACAGTCGTTCTCCCAAAAGTCATGGGCGAGCTTGCTTAAAACGGCGGCAGCGTTTTGACGCTGCGCTCTGCTTGAATCTTTCTCATTGACGTACTCGTTATACTCTTTAAAAGTTGATCCACTGAGAGATTGTGACTGCGATGTTCTACGTGGAGTATAGTAACTAGCAGGATTGAGCCTCCTGCACTCTTTGAGCCTCAATAGAGAAGCGATCTCCTCGGAGAGTTTTCTTCCC                                                                                                                                                   >  SRR13385771.1644092/1‑301 (MQ=60)
                                                                                                                                     TTAAGAGATGCAATCATGATTGTTCTTCGTCAGACGAATCTTCGAATATGTCTTCGTCAATGACACAGTCGTTCTCCCAAAAGTCATGGGCGAGCTTGCTTAAAACGGCGGCAGCGTTTTGACGCTGCGCCCTGCTTGAATCTTTCTCATTGACGTACTCGTTATACTCTTTAAAAGTTGATCCACTGAGAGATTGTGACTGCGATGTTCTACGTGGAGTATAGTAACTAGCAGGATTGAGCCTCCTGCACCCTTTGAGCCTCAATATAGACGCGATCTCCTCCGCGAGTTTTCTTCCCTT                                                                                                                                                 >  SRR13385771.1114556/1‑301 (MQ=60)
                                                                                                                                      TAAGAGATGCAATCATGATTGTTCTTCGTCAGACGAATCTTCGAATATGTCTTCGTCAATGACACAGTCGTTCTCCCAAAAGTCATGGGCGAGCTTGCTTAAAACGGCGGCAGCGTTTTGACGCTGCGCTCTGCTTGAATCTTTCTCATTGACGTACTCGTTATACTCTTTAAAAGTTGATCCACTGAGAGATTGTGACTGCGATGTTCTACGTGGAGTATAGTAACTAGCAGGATTGAGCCTCCTGCACTCTTTGAGCCTCAATAGAGAAGCGATCTCCTCGGAGAGTTTTCTTCCCTT                                                                                                                                                 >  SRR13385771.907504/1‑300 (MQ=60)
                                                                                                                                       AAGGGATGCAATCATGATTGTTCGTCGTCAGACGAATCGTCGAATATGTCTTCGTCAATGACACAGTCGTTCTCCCAAAAGTCATGGGCGAGCTTGCTTAAAACGGCGGCAGCGTTTTGACGCTGCGCTCTGCTTGAATCTTGCTCATTGACGTACTCGTTATACTCTTTAAAAGTTGATCCACTGAGAGATTGTGACTGCGATGTTCTACGTGGAGTATAGTAACTAGCAGGATTGAGCCTCCTGCACTCTTTGAGCCTCAATAGAGAAGCGATCTCCTCGGAGAGTTTTCTTCCCTTAT                                                                                                                                               <  SRR13385771.1892288/301‑1 (MQ=60)
                                                                                                                                               CAATCATGATTGTTCTTCGTCAGACGAATCTTCGAATATGTCTTCGTCAATGACACAGTCGTTCTCCCAAAAGTCATGGGCGAGCTTGCTTAAAACGGCGGCAGCGTTTTGACGCTGCGCTCTGCTTGAATCTTTCTCATTGACGTACTCGTTATACTCTTTAAAAGTTGATCCACTGAGAGATTGTGACTGCGATGTTCTACGTGGAGTATAGTAACTAGCAGGATTGAGCCTCCTGCACTCTTTGAGCCTCAATAGAGAAGCGATCTCCTCGGAGAGTTTTCTTCCCTTATGCTGGTGA                                                                                                                                       <  SRR13385771.1523151/301‑1 (MQ=60)
                                                                                                                                                        TTGTTCTTCGTCAGACGAATCTTCGAATATGTCTTCGTCAATGACACAGTCGTTCTCCCAAAAGTCATGGGCGAGCTTGCTTAAAACGGCGGCAGCGTTTTGACGCTGCGCTCTGCTTGAATCTTTCTCATTGACGTACTCGTTATACTCTTTAAAAGTTGATCCACTGAGAGATTGTGACTGCGATGTTCTACGTGGAGTATAGTAACTAGCAGGATTGAGCCTCCTGCACTCTTTGAGCCTCAATAGAGAAGCGATCTCCTCGGAGAGTTTTCTTCCCTTATGCTGGTGACCATCTTT                                                                                                                               >  SRR13385771.1458428/1‑300 (MQ=60)
                                                                                                                                                               TCGTCAGACGAAGCTTCGAATATGTCTTCGTCAATGACACAGTCGTTCTCCCAAAAGTCATGGGCGAGCTTGCTTAAAACGGCGGCAGCGTTTTGACGCTGCGCTCTGCTTGAATCTTTCTCATTGACGTACTCGTTATACTCTTTAAAAGTTGATCCACTGAGAGATTGTGACTGCGATGTTCTACGTTGAGTATAGTAACTAGCAGGATTGAGCCTCCTGCACTCTTTGAGCCTCAATAGAGAAGCGATCTCCTCGGAGAGTTTTCTTCCCTTATGCTGGTGACCATCTTTAAGTTG                                                                                                                         <  SRR13385771.395376/299‑1 (MQ=60)
                                                                                                                                                                  TCAGACGAATCTTCGAATATGTCTTCGTCAATGACACAGTCGTTCTCACAAAAGTCATGGGCGAGCTTGCTTAAAACGGCGGCAGCGTTTTGACGCTGCGCTCTGCTTGCATCTTTCTCATTGACGTACTCGTTATACTCTTTAAAAGTTGATCCACTGAGAGATTGTGACTGCGATGTTCTACGTGGCGTATAGTAACTAGAAGGATTGAGCCTCCTGCACTCTTTGAGCCTCAATAGAGAAGCGCTCTCCTCGGCGAGTTTTATTCCCTTATGCTGGTGACTATCTTTAAGTTGTTTGT                                                                                                                    >  SRR13385771.1632441/1‑301 (MQ=60)
                                                                                                                                                                   CAGACGAATCTGCGAATATGTCTTCTGCAATGACACAGTCGTTCTCCCAGAAGTCATGGGCGAGCTTGCTTAAAACGGCGGCAGCGGTTTGACGCTGCGCTCTGCTTGAATCTTTCTCATTGACGTACTCGTTATACTCTTTAAAAGTTGATCCACTGAGAGATTGTGACTGCGATGTTCTACGTGGAGTATAGTAACTAGCAGGATTGAGCCTCCTGCACTCTTTGAGCCTCAATAGGGAAGCGATCTCCTCGGAGAGTGTTCTTCCCTTATGCTGGTGACCATCTTTAAGTTGTTTGT                                                                                                                    <  SRR13385771.1780911/300‑1 (MQ=60)
                                                                                                                                                                    AGACGAAGCTTCGAATATGTCTTCGTCAATGACACAGTCGTTCTCCCAAAAGTCATGGGCGAGCTTGCTTAAAACGGCGGCAGCGTTTTGACGCTGCGCTCTGCTTGAATCTTTCTCATTGACGTACTCGTTATACTCTTTAAAAGTTTATCCACTGAGAGATTGTGACTGCGATGTTCTACGTGTAGTATAGTAACTAGCAGGATTGAGCCTCCTGCACTCTTTGAGCCTCAATAGAGAAGCGATCTCCTCGGAGAGTTTTCTTCCCTTATGCTGGTGACCATCTTTAAGTTGTTTGTGT                                                                                                                  <  SRR13385771.988764/301‑1 (MQ=60)
                                                                                                                                                                        GAATCTTCGAAGATGTCTTCGTCAATGACACAGTCGTTCTCCCAAAAGTCATGGGCGAGCTTGCTTAAAACGGCGGCAGCGTTTTGACGCTGCGCTCTGCTTGAATCTTTCTCATTGACGTACTCGTTATACTCTTTAAAAGTTGATCCACTGAGAGATTGTGACTGCGATGTTCTACGTGGAGTATAGTAACTAGCAGGATTGAGCCTCCTGCACTCTTTGAGCCTCAATAGAGAAGCGATCTCCTCGGAGAGTTTTCTTCCCTTATGCTGGTGACCATCTTTAAGTTGTTTGTGTTGTC                                                                                                              <  SRR13385771.1271762/301‑1 (MQ=60)
                                                                                                                                                                               CGAATATGTCTTCGTCAATGACACAGTCGTTCTCCCAAAAGTCATGGGCGAGCTTGCTTAAAACGGCGGCAGCGTTTTGACGCTGCGCTCTGCTTGAATCTTTCTCATTGACGTACTCGTTATACTCTTTAAAAGTTGATCCACTGAGAGATTGTGACTGCGATGTTCTACGTGGAGTATAGTAACTAGCAGGATTGAGCCTCCTGCACTCTTTGAGCCTCAATAGAGAAGCGATCTCCTCGGAGAGTTTTCTTCCCTTATGCTGGTGACCATCTTTAAGTTGTTTGTGTTGTCCTTGTG                                                                                                        <  SRR13385771.51488/300‑1 (MQ=60)
                                                                                                                                                                                 AATATGTCTTCGTCAATGACACAGTCGTTCTCCCAAAAGTCATGGGCGAGCTTGCTTAAAACGGCGGCAGCGTTTCGACGCTGCGCTCTGCTTGAATCTTTCTCATTGACGTACTCGTTATACTCTTTAAAAGTTGATCCACTGAGAGATTGTGACTGCGATGTTCTACGTGGAGTATAGTAACTAGCAGGATTGAGCCTCCTGCAATATTTGAGCCTCAATAGAGAAGCGATCTCCTCGGAGAGTTTTCTTCCCTTATGCTGGTGACCATATTTAAGTTGTTTGTGTTGTCCTTGTGAA                                                                                                      >  SRR13385771.1000608/1‑300 (MQ=60)
                                                                                                                                                                                          gcgtcgatggtacatgCGTTCTCCCAAAAGTCATGGTCGAGCTTGCTTAAAACTGCGGCAGCGTTTTTACGCTGTGCTCTTCTTGAATCTTTCTCATTGACGTACTCGTGATACTCTTTAAAAGTTGTTCCACTGTGTGATTGTGACTGCGATGTTCTACGTGGAGTATAGTAACTAGCAGGATTGAGCCGCCTGCACGCTTTGTGCCTCAATAGAGAAGCGTTCTCCTCGGAGAGTTTTCTTCCCTTATGCTGGTGACCATCTTTAAGTTGTTTGTGTTGTCCTTGTGAAACTGCTG                                                                                               <  SRR13385771.1816214/282‑1 (MQ=60)
                                                                                                                                                                                                   ACACAGTCGTTCTCCCAAAAGTCATGGGCGAGCTTGCTTAAAACGGCGGCAGCGTTTTGACGCTGCGCTCTGCTTGAATCTTTCTCATTGACGTACTCGTTATACTCTTTAAAAGTTGATCCACTGAGAGATTGTGACTGCGATGTTCTACGTGGAGTATAGTAACTAGCAGGATTGAGCCTCCTGCACTCTTTGAGCCTCAATAGAGAAGCGATCTCCTCGGAGAGTTTTCTTCCCTTATGCTGGTGACCATCTTTAAGTTGTTTGTGTTGTCCTTGTGA                                                                                                       >  SRR13385771.1006973/1‑281 (MQ=60)
                                                                                                                                                                                                                         CATGGGCGAGCTTGCTTAAAACGGCGGCAGCGTTTTGACGCTGCGCTCTGCTTGAATCTTTCTCATTGACGTACTCGTTATACTCTTTAAAAGTTGATCCACTGAGAGATTGTGACTGCGATGTTCTACGTGGAGTATAGTAACTAGCAGGATTGAGCCTCCTGCACTCTTTGAGCCTCAATAGAGAAGCGATCTCCTCGGAGAGTTTTCTTCCCTTATGCTGGTGACCATCTTTAAGTTGTTTGTGTTGTCCTTGTGAAACTGCTGTTTCCATTTTGCCGCGTGGAGACATCTGTCCAAT                                                             >  SRR13385771.1266177/1‑301 (MQ=60)
                                                                                                                                                                                                                                                        GTTTTGACGCTGCGCTCTGCTTGAATCTTTCTCATTGACGTACTCGTTATACTCTTTAAAAGGTGATCCACTGAGAGATTGTGACTGCGATGTTCTACGTGGAGTATAGTAACTAGCAGGATTGAGCCTCCTGCACTCTTTGAGCCTCAATAGAGAAGCGATCTCCTCGGAGAGTTTTCTTCCCTTATGCTGGTGACCATCTTTAAGTTGTTTGTGTTGTCCTTGTGAAACTGCTGTTTCCATGTTGCCGCGTGGAGACATCTGTCCAATTTGCCCCTGGCTCTCTGTAAATACCCATATA                              <  SRR13385771.1308170/301‑1 (MQ=60)
                                                                                                                                                                                                                                                                GCTGCGCTCTGCTTGAATCTTTCTCATTGACGTACTCGTTATACTCTTTAAAAGTTGATCCACTGAGAGATTGTGACTGCGATGTTCTACGTGGAGTATAGTAACTAGCAGGATTGAGCCTCCTGCACTCTTTGAGCCTCAATAGAGAAGCGATCTCCTCGGAGAGTTTTCTTCCCTTATGCTGGTGCCCATCTTTAAGTTGTTTGTGTTGTCCTTGTGAAACTGCTGTTTCCATTTTGCCGCGTGGAGACCTCTGTCCAATTTGCCCCTGGCTCTCTGTAAATCCCCATATATGAACGTT                      >  SRR13385771.811464/1‑301 (MQ=60)
                                                                                                                                                                                                                                                                GCTGCGCTCTGCTTGAAGCTTTCTCATTGTCGGACTCGTTATACTCTTTAAAAGTTGATCCACTGAGAGATTGTGACTGCGATGTTCTACGTGGAGTATAGTAACTAGCAGGATTGAGCCTCCTGCACTCTTTGAGCCTCAATAGAGAAGCGATCTCCGCGGATAGTTTTCTTCCCTTATGCTTGTTACCATCTTTAAGTTGTTTGTGTTGTCCTTGTGAAACTGCTGTTTCCATTTTGCCGCGTGGAGACATCTGGCCAATTTGCCCCTGGCTCTCTGTAAATACCCATATATGAACGTT                      <  SRR13385771.1136709/301‑1 (MQ=60)
                                                                                                                                                                                                                                                                     GCTCTGCTTGAATCTTTCTCATTGACGTACTCGTTATACTCTTTAAAAGTTGATCCACTGAGAGATTGTGACTGCGATGTTCTACGTGGAGTATCGTAACTAGCAGGATTGAGCCTCCTGCACTCTTTGAGCCTCAATAGAGCAGCGATCTCCTCGGAGAGTTTTCTTCCCTTATTCTGGTGACCATCTTTAAGTTGTTTGTGTTGTCCTTGTGAAACTGCTGTTTCCATTTTGCCGCGTGGCGCCATCTGTCCAATTTGCCCCTGGCTCTCTGTAAAT                                       >  SRR13385771.1313711/1‑279 (MQ=60)
                                                                                                                                                                                                                                                                      CTCTGCTTGAATCTTTCTCATTGACGTACTCGTTATACTCTTTAAAAGTTGATCCACTGAGAGATTGTGACTGCGATGTTCTACGTGGAGTATAGTAACTAGCAGGATTGAGCCTCCTGCACTCTTTGAGCCTCAATAGAGAAGCGATCTCCTCGGAGAGTTTTCTTCCCTTATGCTGGTGACCATCTTTAAGTTGTTTGTGTTGTCCTTGTGAAACTGCTGTTTCCATTTTGCCGCGTGGAGACATCTGTCCAATTTGCCCCTGGCTCTCTGTAAATACCCATATATGAACGTTATATAT                >  SRR13385771.303302/1‑301 (MQ=60)
                                                                                                                                                                                                                                                                        CTGCTTGAATCTTTCTCATTGACGTACTCGTTATACTCTTTAAAAGTTGATCCACTGAGAGATTGTGACTGCGATGTTCTACGTGGAGTATAGTAACTAGCAGGATTGAGCCTCCTGCACTCTTTGAGCCTCAATAGAGAAGCGATCTCCTCGGAGAGTTTTCTTCCCTTATGCTGGTGACCATCTTTAAGTTGTTTGTGTTGTCCTTGTGAAACTGCTGTTTCCATTTTGCCGCGTGGAGACATCTGTCCAATTTGCCCCTGGCTCTCTGTAAATACCCATATATGAACGTTATATAT                <  SRR13385771.184733/299‑1 (MQ=60)
                                                                                                                                                                                                                                                                                     TCTCATTGACGTACTCGTTATACGCTGTAAAAGTTGATCCACTGAGAGAGTGTGACTGCGATGTTCTACTTGGAGTATAGTAACTAGCAGGATTTAGCCTCCTGCACTCTTTGAGCCGCAATAGAGAAGCGATCTCCTCGGAGAGTTTTCTTCCCTTATGCTGGTGACCATCTTTAAGTTGTTTGTGTTGTCCTGGGGAAACTGCTGTTTCCATTTTGCCGCGTGGAGACATCTGGCCAATTTGCCCCTGGCTCTCTTTAAATACCCATATATGAACGTTATATATATTGTATATTAATG  <  SRR13385771.1869415/300‑1 (MQ=60)
                                                                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                                                                                    
TCCGGCGAAAAAATCGTAAGAAACGAAAGGAGAACGAGCCCACAGGCGGGGTAACATCAAAAGGATTTATATTAGATCGTAAAGTCAATGCATATGTAACAGTACAGAGGTAGAAGGTATGTATGTGTAACGATTAAGAGATGCAATCATGATTGTTCTTCGTCAGACGAATCTTCGAATATGTCTTCGTCAATGACACAGTCGTTCTCCCAAAAGTCATGGGCGAGCTTGCTTAAAACGGCGGCAGCGTTTTGACGCTGCGCTCTACTTGAATCTTTCTCATTAATGTACTCGTTATACTCTTTAAAAGTTGATCCACTGAGAGATTGTGACTGCGATGTTCTACGTGGAGTATAGTAACTAGCAGGATTGAGCCTCCTGCACTCTTTGAGCCTCAATAGAGAAGCGATCTCCTCGGAGAGTTTTCTTCCCTTATGCTGGTGACCATCTTTAAGTTGTTTGTGTTGTCCTTGTGAAACTGCTGTTTCCATTTTGCCGCGTGGAGACATCTGTCCAATTTGCCCCTGGCTCTCTGTAAATACCCATATATGAACGTTATATATATTGTATATTAATG  >  BK006934/374508‑375084

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑