Notes

Publication:

10.1016/j.ymben.2023.01.012

ALE, flask, and isolate counts

ALE replicate Flask count Isolate count
1 2 2
2 1 1
3 1 1
4 1 1
5 1 1
6 1 1
6 7 7

ALE experiment mutation counts

Mutation Type Unique Observed
Single Base Substitutions 14 19
Multiple Base Substitutions 0 0
Deletions 11 21
Insertions 2 8
Mobile Element Insertions 4 5
Amplifications 434 459
Gene Conversions 0 0
Inversions 0 0
Total 465 512

Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Unassigned Missing Coverage Evidence Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F0 I1 R1 70 0 97.7 3889676 92.5% 3597950 140.6
A1 F82 I1 R1 79 0 139.0 5478308 95.5% 5231784 140.8
A2 F82 I1 R1 93 0 99.7 3955078 95.1% 3761279 140.8
A3 F82 I1 R1 81 0 130.1 5150230 95.7% 4928770 140.8
A4 F82 I1 R1 88 0 101.6 4053650 95.1% 3855021 140.8
A5 F82 I1 R1 91 0 98.0 3929856 94.8% 3725503 140.8
A6 F85 I1 R1 93 0 109.1 4340046 95.3% 4136063 140.8

Mutation Needle Plot

Mutation Targets

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