breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
MC JC 257,908 Δ776 bp insB9[crl] insB9, insA9, [crl]
RA 701,380 C→T G72E (GGG→GAG)  nagC ← DNA‑binding transcriptional dual regulator NagC
RA 1,529,218 C→T intergenic (+2066/‑704) yncH → / → ydcD DUF5445 domain‑containing protein YncH/uncharacterized protein YdcD
JC JC 1,642,200 IS5 (+) +4 bp coding (79‑82/90 nt) ynfS → Qin prophage; protein YnfS
MC JC 1,978,503 Δ776 bp insB5insA5 insB5, insA5
RA 2,173,363 Δ2 bp intergenic (‑490/+918) gatD ← / ← gatB galactitol‑1‑phosphate 5‑dehydrogenase/galactitol‑specific PTS enzyme IIB component
MC JC 2,801,828 Δ10,193 bp [nrdE][emrA] [nrdE], nrdF, proV, proW, proX, ygaZ, ygaH, mprA, [emrA]
RA 3,560,455 +G intergenic (+589/+167) rtcR → / ← glpG DNA‑binding transcriptional activator RtcR/rhomboid protease GlpG
JC 3,815,859 Δ82 bp [rph] [rph]
RA 4,037,265 T→C noncoding (6/76 nt) alaT → tRNA‑Ala
RA 4,037,291 T→C noncoding (32/76 nt) alaT → tRNA‑Ala
RA 4,037,308 T→A noncoding (49/76 nt) alaT → tRNA‑Ala
RA 4,037,324 A→T noncoding (65/76 nt) alaT → tRNA‑Ala
RA 4,037,326 A→G noncoding (67/76 nt) alaT → tRNA‑Ala
RA 4,181,849 G→T R202L (CGT→CTT)  rpoB → RNA polymerase subunit beta
RA 4,296,381 +GC intergenic (+587/+55) gltP → / ← yjcO glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 3423792–3424234 3424519–3424238 5–728 24 [10] [15] 16 [rrlD] [rrlD]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 374921 =116 (1.080)36 (0.490) 9/194 2.0 25.0% intergenic (+40/‑538) mhpE/mhpT 4‑hydroxy‑2‑oxovalerate aldolase/3‑hydroxyphenylpropionate/3‑ hydroxycinnamate:H(+) symporter
?NC_000913 377531 = 136 (1.830)intergenic (+220/+4) yaiL/frmB DUF2058 domain‑containing protein YaiL/S‑formylglutathione hydrolase FrmB
* ? NC_000913 1207790 =12 (0.110)57 (0.600) 30/248 0.5 69.7% coding (290/630 nt) ycfK e14 prophage; protein StfP
?NC_000913 1209619 = 39 (0.410)intergenic (‑500/‑66) tfaE/pinE e14 prophage; putative tail fiber assembly protein TfaE/e14 prophage; site‑specific DNA recombinase
* ? NC_000913 = 120780512 (0.110)76 (0.800) 30/248 0.5 75.4% coding (305/630 nt) ycfK e14 prophage; protein StfP
?NC_000913 = 1209602 39 (0.410)intergenic (‑483/‑83) tfaE/pinE e14 prophage; putative tail fiber assembly protein TfaE/e14 prophage; site‑specific DNA recombinase
* ? NC_000913 = 12994982 (0.020)69 (0.660) 36/272 0.4 98.6% intergenic (+253/‑69) ychE/insH21 MarC family putative inner membrane protein YchE/IS5 transposase and trans‑activator
?NC_000913 1300698 = 0 (0.000)intergenic (+151/‑484) insH21/oppA IS5 transposase and trans‑activator/oligopeptide ABC transporter periplasmic binding protein
* ? NC_000913 = 3428664160 (1.490)62 (0.770)
+35 bp
18/210 1.0 34.1% noncoding (99/1542 nt) rrsD 16S ribosomal RNA
?NC_000913 = 4035603 NA (NA)noncoding (73/1542 nt) rrsA 16S ribosomal RNA
* ? NC_000913 4037260 =0 (0.000)11 (0.140)
+35 bp
6/210 3.0 25.3% noncoding (1/76 nt) alaT tRNA‑Ala
?NC_000913 = 4175818 87 (0.810)noncoding (65/76 nt) thrT tRNA‑Thr