breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
MC JC 257,908 Δ776 bp insB9[crl] insB9, insA9, [crl]
RA 537,971 A→T G113G (GGA→GGT ybbW → putative allantoin transporter
RA 939,935 G→A A170T (GCT→ACT)  serS → serine‑‑tRNA ligase
RA 1,529,218 C→T intergenic (+2066/‑704) yncH → / → ydcD DUF5445 domain‑containing protein YncH/uncharacterized protein YdcD
JC JC 1,907,482 IS1 (+) +8 bp coding (103‑110/144 nt) yobF ← DUF2527 domain‑containing protein YobF
MC JC 1,978,503 Δ776 bp insB5insA5 insB5, insA5
RA 2,173,363 Δ2 bp intergenic (‑490/+918) gatD ← / ← gatB galactitol‑1‑phosphate 5‑dehydrogenase/galactitol‑specific PTS enzyme IIB component
RA 2,562,251 G→A L47L (TTG→TTA yffO → CPZ‑55 prophage; uncharacterized protein YffO
RA 2,563,390 T→G intergenic (+273/‑202) yffP → / → yffQ CPZ‑55 prophage; uncharacterized protein YffP/CPZ‑55 prophage; uncharacterized protein YffQ
RA 3,560,455 +G intergenic (+589/+167) rtcR → / ← glpG DNA‑binding transcriptional activator RtcR/rhomboid protease GlpG
JC 3,815,859 Δ82 bp [rph] [rph]
RA 3,968,971 Δ1 bp coding (1056/1104 nt) rfe → UDP‑N‑acetylglucosamine‑‑undecaprenyl‑phosphate N‑acetylglucosaminephosphotransferase
RA 4,181,833 C→G R197G (CGT→GGT)  rpoB → RNA polymerase subunit beta
RA 4,296,381 +GC intergenic (+587/+55) gltP → / ← yjcO glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 3423788–3424524 3424524 1–737 32 [29] [29] 32 [rrlD] [rrlD]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 374921 =209 (1.470)43 (0.440) 12/194 2.6 23.9% intergenic (+40/‑538) mhpE/mhpT 4‑hydroxy‑2‑oxovalerate aldolase/3‑hydroxyphenylpropionate/3‑ hydroxycinnamate:H(+) symporter
?NC_000913 377531 = 129 (1.310)intergenic (+220/+4) yaiL/frmB DUF2058 domain‑containing protein YaiL/S‑formylglutathione hydrolase FrmB
* ? NC_000913 = 12994980 (0.000)110 (0.790) 54/272 0.2 100% intergenic (+253/‑69) ychE/insH21 MarC family putative inner membrane protein YchE/IS5 transposase and trans‑activator
?NC_000913 1300698 = 0 (0.000)intergenic (+151/‑484) insH21/oppA IS5 transposase and trans‑activator/oligopeptide ABC transporter periplasmic binding protein
* ? NC_000913 = 3428664196 (1.370)59 (0.550)
+35 bp
20/210 1.6 28.7% noncoding (99/1542 nt) rrsD 16S ribosomal RNA
?NC_000913 = 4035603 NA (NA)noncoding (73/1542 nt) rrsA 16S ribosomal RNA