Notes

Publication:

10.1101/634105

10.1016/j.ymben.2023.01.012

ALE, flask, and isolate counts

ALE replicate Flask count Isolate count
0 1 1
1 1 2
2 1 3
3 1 3
4 1 2
5 1 2
6 1 3
7 1 2
8 1 3
9 9 21

ALE experiment mutation counts

Mutation Type Unique Observed
Single Base Substitutions 417 472
Multiple Base Substitutions 0 0
Deletions 13 51
Insertions 3 3
Mobile Element Insertions 15 28
Amplifications 0 0
Gene Conversions 0 0
Inversions 0 0
Total 448 554

Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Unassigned Missing Coverage Evidence Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A0 F0 I1 R1 4 2 422.4 11956043 98.9% 11824526 164.0
A1 F50 I1 R1 13 4 63.9 1983895 99.3% 1970007 150.4
A1 F50 I2 R1 14 5 79.0 2445193 99.5% 2432967 150.5
A2 F50 I1 R1 9 4 62.0 1921949 99.3% 1908495 150.4
A2 F50 I2 R1 9 4 77.7 2403278 99.5% 2391261 150.5
A2 F50 I3 R1 9 4 75.0 2318054 99.5% 2306463 150.5
A3 F50 I1 R1 13 6 76.1 2358193 99.5% 2346402 150.5
A3 F50 I2 R1 15 6 66.4 2061178 99.5% 2050872 150.5
A3 F50 I3 R1 11 4 76.4 2369272 99.4% 2355056 150.4
A4 F50 I1 R1 5 3 66.4 2060253 99.3% 2045831 150.4
A4 F50 I2 R1 15 4 62.5 1936854 99.5% 1927169 150.5
A5 F50 I1 R1 239 3 60.4 1873331 99.4% 1862091 150.4
A5 F50 I2 R1 192 3 59.7 1852022 99.4% 1840909 150.4
A6 F50 I1 R1 12 4 69.2 2144601 99.4% 2131733 150.5
A6 F50 I2 R1 13 4 127.2 3943980 99.4% 3920316 150.5
A6 F50 I3 R1 12 4 62.9 1957506 99.1% 1939888 150.4
A7 F50 I1 R1 12 5 59.2 1833447 99.3% 1820612 150.4
A7 F50 I2 R1 11 4 64.6 2012779 99.1% 1994663 150.4
A8 F50 I1 R1 10 4 64.6 2002818 99.4% 1990801 150.4
A8 F50 I2 R1 10 3 72.3 2240532 99.6% 2231569 150.5
A8 F50 I3 R1 10 3 69.5 2158647 99.3% 2143536 150.5

Mutation Needle Plot

Mutation Targets

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