breseq  version 0.29.0  revision 8f9c342918e4
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
RA 38,511 T→G E202A (GAA→GCA)  caiB ← crotonobetainyl CoA:carnitine CoA transferase
RA 46,153 T→G I116S (ATC→AGC)  yaaU → putative MFS sugar transporter; membrane protein
RA 73,316 T→G Q402H (CAA→CAC thiP ← thiamine/thiamine pyrophosphate ABC transporter permease
RA 76,552 T→G I250L (ATC→CTC)  sgrR ← transcriptional DNA‑binding transcriptional activator of sgrS sRNA
RA 99,341 G→T V313V (GTG→GTT ftsW → putative lipid II flippase; integral membrane protein; FtsZ ring stabilizer
RA 111,305 G→T E88* (GAA→TAA)  mutT → dGTP‑preferring nucleoside triphosphate pyrophosphohydrolase
RA 136,062 T→G K135Q (AAG→CAG)  speE ← spermidine synthase (putrescine aminopropyltransferase)
RA 141,869 T→G S147A (TCG→GCG)  hpt → hypoxanthine phosphoribosyltransferase
RA 180,550 T→G P438P (CCT→CCG dgt → deoxyguanosine triphosphate triphosphohydrolase
RA 223,342 T→G D170E (GAT→GAG gmhB → D,D‑heptose 1,7‑bisphosphate phosphatase
MC JC 257,908 Δ776 bp [crl] [crl]
RA 289,154 A→C I3M (ATT→ATG yagI ← CP4‑6 prophage; putative DNA‑binding transcriptional regulator
RA 339,705 A→C K461Q (AAA→CAA)  yahG → DUF1116 family protein
RA 348,568 A→G intergenic (‑125/‑114) prpR ← / → prpB propionate catabolism operon regulatory protein/2‑methylisocitrate lyase
RA 351,204 A→C intergenic (+23/‑11) prpC → / → prpD 2‑methylcitrate synthase/2‑methylcitrate dehydratase
RA 368,831 A→C D74A (GAT→GCT)  mhpA → 3‑(3‑hydroxyphenyl)propionate hydroxylase
RA 409,800 T→G I74L (ATC→CTC)  rdgC ← nucleoid‑associated ssDNA and dsDNA binding protein; competitive inhibitor of RecA function
RA 435,380 A→C K82Q (AAA→CAA)  nusB → transcription antitermination protein
RA 473,347 T→G S128A (TCG→GCG)  amtB → ammonium transporter
RA 531,370 A→C D284E (GAT→GAG allS ← allD operon transcriptional activator
RA 534,927 A→C K338Q (AAA→CAA)  gcl → glyoxylate carboligase
RA 573,949 T→G intergenic (+79/‑7) ylcG → / → quuD uncharacterized protein, DLP12 prophage/DLP12 prophage; putative antitermination protein
RA 601,318 A→C N869H (AAC→CAC)  cusA → copper/silver efflux system, membrane component
RA 629,546 A→C T83P (ACA→CCA)  entH → enterobactin synthesis proofreading thioesterase
RA 652,430 T→G S66A (TCC→GCC)  citA → sensory histidine kinase in two‑component regulatory system with CitB
RA 677,883 T→G L157V (TTA→GTA)  djlB → putative HscC co‑chaperone, uncharacterized J domain‑containing protein
RA 714,075 T→G V173G (GTG→GGG)  pgm → phosphoglucomutase
RA 739,405 T→G R135R (CGT→CGG ybgA → DUF1722 family protein
RA 765,808 A→C L23R (CTT→CGT)  mngR ← transcriptional repressor for the mannosyl‑D‑glycerate catabolic operon
RA 794,554 A→C S31A (TCC→GCC)  modE ← transcriptional repressor for the molybdenum transport operon modABC
RA 867,196 A→C D210A (GAT→GCT)  iaaA → Isoaspartyl peptidase
RA 869,818 A→C E136D (GAA→GAC gsiB → glutathione ABC transporter periplasmic binding protein
RA 888,948 T→G E291A (GAA→GCA)  ybjL ← putative transporter
RA 891,141 A→C N77H (AAC→CAC)  ybjC → DUF1418 family protein
RA 901,646 A→C F202C (TTT→TGT)  artQ ← arginine ABC transporter permease
RA 961,445 T→G L82R (CTC→CGC)  cmk → cytidylate kinase
RA 987,537 A→C intergenic (‑555/+48) ompF ← / ← asnS outer membrane porin 1a (Ia;b;F)/asparaginyl tRNA synthetase
RA 987,938 A→C F350V (TTT→GTT)  asnS ← asparaginyl tRNA synthetase
RA 994,244 T→G I197L (ATT→CTT)  ssuC ← aliphatic sulfonate ABC transporter permease
RA 1,021,095 A→C intergenic (‑176/‑43) sulA ← / → sxy SOS cell division inhibitor/CRP‑S‑dependent promoter expression factor
RA 1,052,447 T→G K306Q (AAA→CAA)  yccM ← putative 4Fe‑4S membrane protein
RA 1,098,052 A→C D56A (GAT→GCT)  ghrA → glyoxylate/hydroxypyruvate reductase A
RA 1,104,872 T→G F142L (TTT→TTG csgA → curlin subunit, amyloid curli fibers, cryptic
RA 1,133,235 T→G I221S (ATT→AGT)  flgE → flagellar hook protein
RA 1,145,255 A→C N106H (AAT→CAT)  rluC → 23S rRNA pseudouridine(955,2504,2580) synthase
RA 1,184,728 T→G K289N (AAA→AAC potA ← spermidine/putrescine ABC transporter ATPase
RA 1,194,916 T→G Q12H (CAA→CAC rluE ← 23S rRNA pseudouridine(2457) synthase
RA 1,201,688 A→C T64T (ACA→ACC ymfI → e14 prophage; uncharacterized protein
RA 1,214,780 A→C L232L (CTT→CTG bluF ← anti‑repressor for YcgE, blue light‑responsive; FAD‑binding; inactive c‑di‑GMP phosphodiesterase‑like EAL domain protein
RA 1,253,248 T→G N902T (AAC→ACC)  ycgV ← putative adhesin
RA 1,265,930 A→C N307H (AAC→CAC)  prfA → peptide chain release factor RF‑1
RA 1,296,022 A→C R700R (CGT→CGG adhE ← fused acetaldehyde‑CoA dehydrogenase/iron‑dependent alcohol dehydrogenase/pyruvate‑formate lyase deactivase
RA 1,317,315 A→C D367E (GAT→GAG trpB ← tryptophan synthase, beta subunit
RA 1,324,533 A→C M146L (ATG→CTG)  yciO → putative RNA binding protein
RA 1,374,400 T→G F104C (TTT→TGT)  ycjP → putative sugar ABC transporter permease
RA 1,428,685 A→C T14T (ACT→ACG insH1 ← IS5 transposase and trans‑activator
RA 1,453,097 T→G P182P (CCA→CCC paaZ ← oxepin‑CoA hydrolase and 3‑oxo‑5,6‑dehydrosuberyl‑CoA semialdehyde dehydrogenase
RA 1,465,307 A→C intergenic (‑77/‑85) ynbG ← / → ydbA uncharacterized protein/pseudogene, autotransporter homolog; interrupted by IS2 and IS30
RA 1,492,314 T→G intergenic (‑185/‑156) mokB ← / → trg regulatory peptide/methyl‑accepting chemotaxis protein III, ribose and galactose sensor receptor
RA 1,498,172 A→C E439D (GAA→GAC opgD → OPG biosynthetic periplasmic protein
RA 1,515,898 T→G V107V (GTT→GTG patD → gamma‑aminobutyraldehyde dehydrogenase
RA 1,523,163 A→C intergenic (‑98/‑144) yncD ← / → yncE putative iron outer membrane transporter/ATP‑binding protein, periplasmic, function unknown
RA 1,607,228 A→C K77T (AAA→ACA)  lsrG → autoinducer‑2 (AI‑2) degrading protein LsrG
RA 1,619,441 T→G C108G (TGT→GGT)  marR → transcriptional repressor of multiple antibiotic resistance
RA 1,621,483 T→G I51S (ATT→AGT)  ydeE → putative transporter
RA 1,657,342 A→C F39C (TTT→TGT)  ynfC ← UPF0257 family lipoprotein
RA 1,658,755 T→G G229G (GGT→GGG ynfE → putative selenate reductase, periplasmic
RA 1,676,640 T→G K422Q (AAA→CAA)  pntA ← pyridine nucleotide transhydrogenase, alpha subunit
RA 1,755,502 T→G intergenic (‑361/‑196) ydhZ ← / → pykF uncharacterized protein/pyruvate kinase I
RA 1,804,989 T→G pseudogene (1472/1476 nt) arpB → pseudogene, ankyrin repeats
RA 1,837,673 A→C V154V (GTA→GTC ynjC → putative ABC transporter permease
RA 1,842,229 T→G intergenic (‑94/‑142) ynjH ← / → gdhA DUF1496 family protein/glutamate dehydrogenase, NADP‑specific
RA 1,856,690 T→G E80A (GAG→GCG)  ydjH ← putative kinase
RA 1,870,696 T→G I104M (ATT→ATG yeaI → putative membrane‑anchored diguanylate cyclase
RA 1,898,641 T→G L72R (CTT→CGT)  yoaD → putative membrane‑anchored cyclic‑di‑GMP phosphodiesterase, regulator of cellulose production
RA 1,930,199 T→G K64N (AAA→AAC yebF ← extracellular Colicin M immunity family protein
RA 1,935,635 T→G D227A (GAT→GCT)  zwf ← glucose‑6‑phosphate 1‑dehydrogenase
MC JC 1,978,503 Δ776 bp insB1insA insB1, insA
RA 1,994,716 A→C L215* (TTA→TGA)  uvrY ← response regulator in two‑component regulatory system with BarA
RA 1,999,514 T→G intergenic (‑34/+71) dcyD ← / ← fliY D‑cysteine desulfhydrase, PLP‑dependent/cystine transporter subunit
RA 2,013,101 T→G intergenic (‑87/‑128) fliE ← / → fliF flagellar basal‑body component/flagellar basal‑body MS‑ring and collar protein
RA 2,016,025 A→C E53A (GAG→GCG)  fliH → negative regulator of FliI ATPase activity
RA 2,021,189 T→G A34A (GCT→GCG fliN → flagellar motor switching and energizing component
RA 2,062,145 A→C intergenic (‑212/‑115) nac ← / → asnV nitrogen assimilation regulon transcriptional regulator; autorepressor/tRNA‑Asn
RA 2,085,613 A→C intergenic (‑88/+91) yeeE ← / ← plaP UPF0394 family inner membrane protein/putrescine importer, low affinity
RA 2,098,442 A→C intergenic (‑141/+5) wzzB ← / ← ugd regulator of length of O‑antigen component of lipopolysaccharide chains/UDP‑glucose 6‑dehydrogenase
RA 2,107,808 T→G R174S (AGA→AGC glf ← UDP‑galactopyranose mutase, FAD/NAD(P)‑binding
RA 2,124,845 A→C L301R (CTG→CGG)  wcaI ← putative glycosyl transferase
RA 2,173,363 Δ2 bp intergenic (‑1/+1) gatC ← / ← gatC pseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC/pseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC
RA 2,312,206 A→C F182V (TTT→GTT)  ompC ← outer membrane porin protein C
RA 2,321,945 A→C Q27P (CAA→CCA)  atoC → fused response regulator of ato operon, in two‑component system with AtoS: response regulator/sigma54 interaction protein
RA 2,348,730 T→G C73G (TGT→GGT)  yfaE → ferredoxin involved with ribonucleotide reductase diferric‑tyrosyl radical (Y*) cofactor maintenance
RA 2,356,017 T→G F166C (TTT→TGT)  glpC → anaerobic sn‑glycerol‑3‑phosphate dehydrogenase, C subunit, 4Fe‑4S iron‑sulfur cluster
RA 2,376,074 A→C L249R (CTG→CGG)  menB ← dihydroxynaphthoic acid synthetase
RA 2,380,295 A→C C117G (TGT→GGT)  menF ← isochorismate synthase 2
RA 2,438,570 A→C P207P (CCA→CCC flk → putative flagella assembly protein
RA 2,486,868 T→G V832G (GTC→GGC)  evgS → hybrid sensory histidine kinase in two‑component regulatory system with EvgA
RA 2,492,875 A→C F127C (TTT→TGT)  frc ← formyl‑CoA transferase, NAD(P)‑binding
RA 2,517,660 A→C I58S (ATC→AGC)  yfeA ← putative diguanylate cyclase
RA 2,521,793 T→G N229H (AAT→CAT)  xapR ← transcriptional activator of xapAB
RA 2,546,546 T→G I258M (ATT→ATG murQ → N‑acetylmuramic acid 6‑phosphate (MurNAc‑6‑P) etherase
RA 2,593,811 A→C intergenic (‑41/+33) ypfH ← / ← tmcA palmitoyl‑CoA esterase activity, uncertain physiological substrate/elongator methionine tRNA (ac4C34) acetyltransferase
RA 2,615,642 T→G I80I (ATA→ATC yfgO ← putative UPF0118 family inner membrane permease
RA 2,615,883 A→C intergenic (‑2/‑211) yfgO ← / → bepA putative UPF0118 family inner membrane permease/OM protein maintenance and assembly metalloprotease and chaperone, periplasmic
RA 2,648,982 A→C I1102M (ATT→ATG yfhM ← bacterial alpha2‑macroglobulin colonization factor ECAM; anti‑host protease defense factor; periplasmic inner membrane‑anchored lipoprotein
RA 2,702,600 T→G K263Q (AAA→CAA)  era ← membrane‑associated, 16S rRNA‑binding GTPase
RA 2,704,662 T→G E216D (GAA→GAC lepB ← leader peptidase (signal peptidase I)
RA 2,724,576 A→C Y391D (TAC→GAC)  kgtP ← alpha‑ketoglutarate transporter
RA 2,732,833 T→G E447D (GAA→GAC clpB ← protein disaggregation chaperone
RA 2,753,923 A→C K107Q (AAA→CAA)  bamE → lipoprotein component of BamABCDE OM biogenesis complex
RA 2,761,653 A→C L630V (TTG→GTG)  yfjK ← radiation resistance protein; DEAD/H helicase‑like protein; CP4‑57 putative defective prophage
RA 2,766,683 A→C T256P (ACC→CCC)  rnlA → CP4‑57 prophage; RNase LS
RA 2,778,122 A→C intergenic (+340/+24) ypjF → / ← ypjA CP4‑57 prophage; toxin of the YpjF‑YfjZ toxin‑antitoxin system/adhesin‑like autotransporter
RA 2,799,511 A→C S116S (TCA→TCC alaE → alanine exporter, alanine‑inducible, stress‑responsive
RA 2,805,674 T→G L287* (TTA→TGA)  proV → glycine betaine/proline ABC transporter periplasmic binding protein
RA 2,806,796 A→C T263P (ACC→CCC)  proW → glycine betaine/proline ABC transporter permease
RA 2,815,599 T→G K281Q (AAA→CAA)  gshA ← glutamate‑cysteine ligase
RA 2,822,103 A→C intergenic (‑92/+36) alaS ← / ← recX alanyl‑tRNA synthetase/regulatory protein for RecA
RA 2,863,239 T→G L302R (CTG→CGG)  ygbK → FliA‑regulated DUF1537 family protein
RA 3,015,808 T→G intergenic (+70/‑252) mocA → / → ygfK CTP:molybdopterin cytidylyltransferase/putative Fe‑S subunit oxidoreductase subunit
RA 3,027,187 A→C K23Q (AAA→CAA)  ghxQ → guanine/hypoxanthine permease, high affinity; guanine/hypoxanthine:H+ symporter
RA 3,062,456 A→C E536A (GAA→GCA)  scpA → methylmalonyl‑CoA mutase
RA 3,075,015 T→G S61R (AGC→CGC)  yggD ← MtlR family putative transcriptional repressor
RA 3,084,833 T→G E360A (GAA→GCA)  speA ← biosynthetic arginine decarboxylase, PLP‑binding
RA 3,093,773 A→C K92Q (AAG→CAG)  yqgF → putative Holliday junction resolvase
RA 3,268,329 T→G I159S (ATT→AGT)  yhaB → uncharacterized protein
RA 3,269,279 A→C N289H (AAC→CAC)  yhaC → pentapetide repeats‑related protein
RA 3,282,649 A→C K225T (AAA→ACA)  agaS → tagatose‑6‑phosphate ketose/aldose isomerase
RA 3,310,939 A→C F77C (TTC→TGC)  pnp ← polynucleotide phosphorylase/polyadenylase
RA 3,343,061 T→G S76A (TCG→GCG)  lptC → periplasmic membrane‑anchored LPS‑binding protein; LPS export protein
RA 3,350,729 T→G E766A (GAA→GCA)  arcB ← aerobic respiration control sensor histidine protein kinase, cognate to two‑component response regulators ArcA and RssB
RA 3,365,608 T→G intergenic (+57/+94) yhcE → / ← insH1 pseudogene fragment; interrupted by IS5/IS5 transposase and trans‑activator
RA 3,377,387 T→G T12P (ACG→CCG)  sspA ← stringent starvation protein A, phage P1 late gene activator, RNAP‑associated acid‑resistance protein, inactive glutathione S‑transferase homolog
RA 3,383,818 T→G I151L (ATC→CTC)  mdh ← malate dehydrogenase, NAD(P)‑binding
RA 3,392,795 A→C I1155S (ATT→AGT)  yhdP ← DUF3971‑AsmA2 domains protein
RA 3,422,588 A→C K51N (AAA→AAC yhdZ → putative amino acid ABC transporter ATPase
RA 3,431,682 T→G E94A (GAA→GCA)  yrdD ← ssDNA‑binding protein, function unknown
RA 3,456,471 T→G F32C (TTC→TGC)  gspD → general secretory pathway component, cryptic
RA 3,468,693 T→G T387T (ACA→ACC chiA ← periplasmic endochitinase
RA 3,478,998 T→G H537P (CAT→CCT)  kefB ← potassium:proton antiporter
RA 3,484,500 A→C K4T (AAA→ACA)  prkB → putative phosphoribulokinase
RA 3,493,473 T→G A302A (GCT→GCG tsgA → putative transporter
RA 3,494,086 T→G S26A (TCT→GCT)  nirB → nitrite reductase, large subunit, NAD(P)H‑binding
RA 3,496,373 A→C E788A (GAA→GCA)  nirB → nitrite reductase, large subunit, NAD(P)H‑binding
RA 3,552,399 A→C N25K (AAT→AAG malP ← maltodextrin phosphorylase
RA 3,560,455 +G intergenic (‑2/+1) glpR ← / ← glpR pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon/pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon
RA 3,565,832 A→C G250G (GGT→GGG glgP ← glycogen phosphorylase
RA 3,640,281 T→G D57E (GAT→GAG uspA → universal stress global response regulator
RA 3,651,303 T→G pseudogene (13/741 nt) yhiS → pseudogene
RA 3,666,292 T→G E430A (GAA→GCA)  gadA ← glutamate decarboxylase A, PLP‑dependent
RA 3,760,776 T→G L158F (TTA→TTC selA ← selenocysteine synthase
RA 3,766,728 A→C E131A (GAA→GCA)  yibA → putative immunity protein for polymorphic toxin RhsA; HEAT‑domain protein; lethality reduction protein
RA 3,771,984 A→C intergenic (‑240/‑297) yibI ← / → mtlA DUF3302 family inner membrane protein/mannitol‑specific PTS enzyme: IIA, IIB and IIC components
RA 3,772,302 A→C K8Q (AAA→CAA)  mtlA → mannitol‑specific PTS enzyme: IIA, IIB and IIC components
RA 3,780,450 A→C S208S (TCA→TCC lldD → L‑lactate dehydrogenase, FMN‑linked
RA 3,806,922 A→C F49C (TTT→TGT)  waaG ← UDP‑glucose:(heptosyl)lipopolysaccharide alpha‑1,3‑glucosyltransferase; lipopolysaccharide core biosynthesis protein; lipopolysaccharide glucosyltransferase I
RA 3,833,087 T→G Y484S (TAC→TCC)  yicI ← putative alpha‑glucosidase
RA 3,843,762 T→G S10R (AGT→CGT)  adeQ ← adenine permease, high affinity; adenine:H+ symporter
RA 3,864,289 A→C K131Q (AAA→CAA)  yidP → UTRA domain‑containing GntR family putative transcriptional regulator
RA 3,874,407 T→G D23A (GAC→GCC)  dgoK ← 2‑oxo‑3‑deoxygalactonate kinase
RA 3,898,177 T→G V52V (GTT→GTG cbrC → UPF0167 family protein
RA 3,968,363 A→C N150H (AAT→CAT)  wecA → UDP‑GlcNAc:undecaprenylphosphate GlcNAc‑1‑phosphate transferase
RA 3,970,454 T→G Y108D (TAT→GAT)  wecB → UDP‑N‑acetyl glucosamine‑2‑epimerase
RA 4,005,207 T→G S116A (TCC→GCC)  pldA → outer membrane phospholipase A
RA 4,045,010 A→C E271A (GAA→GCA)  yihF → DUF945 family protein
RA 4,076,337 T→G F64L (TTT→TTG yihY → BrkB family putative transporter, inner membrane protein
RA 4,078,641 A→C intergenic (+203/‑656) yiiD → / → yiiE GNAT family putative N‑acetyltransferase/CopG family putative transcriptional regulator
RA 4,116,511 T→G I238L (ATT→CTT)  glpK ← glycerol kinase
RA 4,152,771 T→G K110Q (AAA→CAA)  ppc ← phosphoenolpyruvate carboxylase
RA 4,179,175 T→G D99E (GAT→GAG rplA → 50S ribosomal subunit protein L1
RA 4,182,877 T→G F545V (TTC→GTC)  rpoB → RNA polymerase, beta subunit
RA 4,187,714 A→C K789Q (AAA→CAA)  rpoC → RNA polymerase, beta prime subunit
RA 4,190,510 T→G intergenic (+185/+225) yjaZ → / ← thiH stationary phase growth adaptation protein/tyrosine lyase, involved in thiamine‑thiazole moiety synthesis
RA 4,217,768 T→G A220A (GCT→GCG aceA → isocitrate lyase
RA 4,237,239 A→C I115L (ATC→CTC)  yjbG → extracellular polysaccharide export OMA protein
RA 4,255,533 A→C Y312D (TAC→GAC)  plsB ← glycerol‑3‑phosphate O‑acyltransferase
RA 4,276,646 T→G F392L (TTT→TTG yjcC → putative membrane‑anchored cyclic‑di‑GMP phosphodiesterase
RA 4,296,381 +GC intergenic (+587/+55) gltP → / ← yjcO glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein
RA 4,304,733 T→G K622T (AAA→ACA)  yjcS ← metallo‑beta‑lactamase superfamily protein
RA 4,313,739 A→C E130D (GAA→GAC rpiB → ribose 5‑phosphate isomerase B/allose 6‑phosphate isomerase
RA 4,368,231 T→G N33H (AAC→CAC)  aspA ← aspartate ammonia‑lyase
RA 4,396,687 T→G I208S (ATC→AGC)  amiB → N‑acetylmuramoyl‑l‑alanine amidase II
RA 4,399,742 A→C D164A (GAT→GCT)  miaA → delta(2)‑isopentenylpyrophosphate tRNA‑adenosine transferase
RA 4,462,366 T→G N99H (AAC→CAC)  nrdD ← anaerobic ribonucleoside‑triphosphate reductase
RA 4,516,206 T→G N158H (AAC→CAC)  fecA ← TonB‑dependent outer membrane ferric citrate transporter and signal transducer; ferric citrate extracelluar receptor; FecR‑interacting protein
RA 4,535,814 T→G pseudogene (812/1029 nt) yjhR → pseudogene, helicase family; putative frameshift suppressor
RA 4,543,958 T→G V77V (GTT→GTG fimI → fimbrial protein involved in type 1 pilus biosynthesis
RA 4,544,628 A→C N109H (AAC→CAC)  fimC → periplasmic chaperone
RA 4,573,896 A→C pseudogene (1483/1503 nt) yjiT → pseudogene
RA 4,584,054 A→C L903R (CTC→CGC)  hsdR ← endonuclease R Type I restriction enzyme
RA 4,586,090 A→C A224A (GCT→GCG hsdR ← endonuclease R Type I restriction enzyme
RA 4,615,786 T→G E427A (GAA→GCA)  yjjI ← DUF3029 family protein, putative glycine radical enzyme

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 3423726–3424234 3424584–3424238 5–859 31 [29] [29] 31 [rrfD]–[rrlD] [rrfD], [rrlD]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 1207790 =43 (0.730)29 (0.550) 28/266 1.8 39.4% coding (290/630 nt) stfP e14 prophage; uncharacterized protein
?NC_000913 1209619 = 51 (0.960)pseudogene (1/501 nt) stfE pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related
* ? NC_000913 = 12994980 (0.000)52 (0.900) 46/290 0.5 100% intergenic (+253/‑1684) ychE/oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein
?NC_000913 1300698 = 0 (0.000)intergenic (+1453/‑484) ychE/oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein