breseq  version 0.29.0  revision 8f9c342918e4
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
RA 22,105 T→G G233G (GGT→GGG ribF → bifunctional riboflavin kinase/FAD synthetase
RA 46,040 T→G D78E (GAT→GAG yaaU → putative MFS sugar transporter; membrane protein
RA 46,153 T→G I116S (ATC→AGC)  yaaU → putative MFS sugar transporter; membrane protein
RA 46,879 A→C Y358S (TAT→TCT)  yaaU → putative MFS sugar transporter; membrane protein
RA 61,420 T→G K615N (AAA→AAC rapA ← RNA polymerase remodeling/recycling factor ATPase; RNA polymerase‑associated, ATP‑dependent RNA translocase
RA 62,084 T→G E394A (GAA→GCA)  rapA ← RNA polymerase remodeling/recycling factor ATPase; RNA polymerase‑associated, ATP‑dependent RNA translocase
RA 83,522 T→G Q3P (CAG→CCG)  leuA ← 2‑isopropylmalate synthase
RA 99,341 G→T V313V (GTG→GTT ftsW → putative lipid II flippase; integral membrane protein; FtsZ ring stabilizer
RA 111,305 G→T E88* (GAA→TAA)  mutT → dGTP‑preferring nucleoside triphosphate pyrophosphohydrolase
RA 126,032 T→G I113S (ATC→AGC)  aceF → pyruvate dehydrogenase, dihydrolipoyltransacetylase component E2
RA 136,062 T→G K135Q (AAG→CAG)  speE ← spermidine synthase (putrescine aminopropyltransferase)
RA 141,194 A→C L11* (TTA→TGA)  gcd ← glucose dehydrogenase
RA 158,000 A→C L376W (TTG→TGG)  pcnB ← poly(A) polymerase
RA 163,110 A→C I336L (ATC→CTC)  hrpB → putative ATP‑dependent helicase
RA 184,410 A→C V220V (GTT→GTG yaeI ← phosphodiesterase with model substrate bis‑pNPP
RA 197,703 T→G I386M (ATT→ATG rseP → inner membrane zinc RIP metalloprotease; RpoE activator, by degrading RseA; cleaved signal peptide endoprotease
RA 204,870 T→G D126E (GAT→GAG rnhB → ribonuclease HII, degrades RNA of DNA‑RNA hybrids
RA 212,003 T→G S43A (TCA→GCA)  yaeR → putative lyase
RA 216,474 A→C F177C (TTC→TGC)  yaeF ← putative lipoprotein
RA 219,876 T→G K40N (AAA→AAC rcsF ← putative outer membrane protein
RA 235,475 A→C K220N (AAA→AAC yafS → putative S‑adenosyl‑L‑methionine‑dependent methyltransferase
RA 243,573 A→C N11H (AAC→CAC)  gmhA → D‑sedoheptulose 7‑phosphate isomerase
RA 256,342 T→G V122V (GTT→GTG gpt → xanthine phosphoribosyltransferase; xanthine‑guanine phosphoribosyltransferase
MC JC 257,908 Δ776 bp [crl] [crl]
RA 266,989 A→C intergenic (‑22/+195) ykfK ← / ← yafZ CP4‑6 prophage; putative DNA‑binding transcriptional regulator;Phage or Prophage Related/CP4‑6 prophage; conserved protein
RA 289,154 A→C I3M (ATT→ATG yagI ← CP4‑6 prophage; putative DNA‑binding transcriptional regulator
RA 353,745 A→C E347A (GAA→GCA)  prpE → propionate‑‑CoA ligase
RA 417,041 T→G intergenic (‑89/‑101) sbcD ← / → phoB exonuclease, dsDNA, ATP‑dependent/response regulator in two‑component regulatory system with PhoR
RA 431,697 A→C F106C (TTC→TGC)  tsx ← nucleoside channel, receptor of phage T6 and colicin K
RA 435,380 A→C K82Q (AAA→CAA)  nusB → transcription antitermination protein
RA 468,115 A→C Q235P (CAA→CCA)  cof → thiamine pyrimidine pyrophosphate hydrolase; HMP‑PP phosphatase
RA 487,353 T→G V273G (GTC→GGC)  mscK → mechanosensitive channel protein, intermediate conductance, K+ regulated
RA 505,546 T→G I211M (ATT→ATG ushA → bifunctional UDP‑sugar hydrolase/5'‑nucleotidase
RA 544,671 T→G N208H (AAT→CAT)  allC ← allantoate amidohydrolase
RA 577,020 A→C intergenic (‑195/‑378) nmpC ← / → essD DLP12 prophage; truncated outer membrane porin (pseudogene);IS, phage, Tn; Phage or Prophage Related; outer membrane porin protein; locus of qsr prophage/DLP12 prophage; putative phage lysis protein
RA 600,653 A→C E647A (GAG→GCG)  cusA → copper/silver efflux system, membrane component
RA 634,387 T→G S268A (TCG→GCG)  ybdL → methionine aminotransferase, PLP‑dependent
RA 716,122 T→G pseudogene (476/651 nt) ybfG ← pseudogene
RA 729,193 T→G V20V (GTT→GTG ybfA → DUF2517 family protein
RA 770,824 T→G intergenic (+213/‑634) mngB → / → cydA alpha‑mannosidase/cytochrome d terminal oxidase, subunit I
RA 772,414 T→G R319R (CGT→CGG cydA → cytochrome d terminal oxidase, subunit I
RA 791,879 A→C I59M (ATT→ATG galE ← UDP‑galactose‑4‑epimerase
RA 808,046 A→C I404M (ATT→ATG bioA ← 7,8‑diaminopelargonic acid synthase, PLP‑dependent
RA 838,474 A→C intergenic (‑18/+56) ybiI ← / ← ybiX DksA‑type zinc finger protein/Fe(II)‑dependent oxygenase superfamily protein
RA 888,480 A→C F447C (TTC→TGC)  ybjL ← putative transporter
RA 902,592 T→G N133H (AAC→CAC)  artI ← arginine transporter subunit
RA 914,569 T→G K97Q (AAA→CAA)  ybjE ← putative transporter
RA 936,254 A→C K1011Q (AAA→CAA)  ftsK → DNA translocase at septal ring sorting daughter chromsomes
RA 960,522 A→C N87H (AAT→CAT)  ycaL → putative peptidase‑related chaperone
RA 997,715 A→C intergenic (‑203/‑153) ssuE ← / → elfA NAD(P)H‑dependent FMN reductase/laminin‑binding fimbrin subunit
RA 1,052,447 T→G K306Q (AAA→CAA)  yccM ← putative 4Fe‑4S membrane protein
RA 1,063,726 T→G D17A (GAT→GCT)  cbpA ← DnaK co‑chaperone; curved DNA‑binding protein
RA 1,133,140 T→G S189R (AGT→AGG flgE → flagellar hook protein
RA 1,157,282 A→C D169A (GAT→GCT)  ycfH → putative DNase
RA 1,194,916 T→G Q12H (CAA→CAC rluE ← 23S rRNA pseudouridine(2457) synthase
RA 1,196,616 A→C intergenic (+243/+251) icd → / ← ymfD isocitrate dehydrogenase; e14 prophage attachment site; tellurite reductase/e14 prophage; putative SAM‑dependent methyltransferase
RA 1,201,688 A→C T64T (ACA→ACC ymfI → e14 prophage; uncharacterized protein
RA 1,202,079 A→C N2K (AAT→AAG ymfJ ← e14 prophage; uncharacterized protein
RA 1,213,848 T→G E71A (GAA→GCA)  bluR ← repressor of blue light‑responsive genes
RA 1,217,327 A→C intergenic (+331/‑1) ymgC → / → ycgG Blue light, low temperature and stress induced protein/putative membrane‑anchored cyclic‑di‑GMP phosphodiesterase
RA 1,217,877 A→C T184P (ACG→CCG)  ycgG → putative membrane‑anchored cyclic‑di‑GMP phosphodiesterase
RA 1,253,248 T→G N902T (AAC→ACC)  ycgV ← putative adhesin
RA 1,263,618 T→G intergenic (‑118/‑96) lolB ← / → hemA lipoprotein localization factor/glutamyl tRNA reductase
RA 1,265,930 A→C N307H (AAC→CAC)  prfA → peptide chain release factor RF‑1
RA 1,277,396 T→G K75Q (AAA→CAA)  narX ← sensory histidine kinase in two‑component regulatory system with NarL
RA 1,317,315 A→C D367E (GAT→GAG trpB ← tryptophan synthase, beta subunit
RA 1,323,663 T→G L148L (CTT→CTG yciV → PHP domain protein
RA 1,334,673 T→G G273G (GGT→GGG cysB → N‑acetylserine‑responsive cysteine regulon transcriptional activator; autorepressor
RA 1,399,602 T→G intergenic (‑76/+119) fnr ← / ← ogt oxygen‑sensing anaerobic growth regulon transcriptional regulator FNR; autorepressor/O‑6‑alkylguanine‑DNA:cysteine‑protein methyltransferase
RA 1,404,625 A→C intergenic (‑60/‑116) abgA ← / → abgR p‑aminobenzoyl‑glutamate hydrolase, A subunit/putative DNA‑binding transcriptional regulator of abgABT operon
RA 1,406,711 A→C V362G (GTG→GGG)  ydaM ← diguanylate cyclase, csgD regulator
RA 1,412,371 T→G K289Q (AAA→CAA)  intR ← Rac prophage; integrase
RA 1,476,087 A→C E315A (GAG→GCG)  ydbD → PF10971 family putative periplasmic methylglyoxal resistance protein
RA 1,486,129 T→G G1023G (GGT→GGG hrpA → putative ATP‑dependent helicase
RA 1,498,172 A→C E439D (GAA→GAC opgD → OPG biosynthetic periplasmic protein
RA 1,508,711 T→G L644R (CTG→CGG)  ydcP → putative peptidase
RA 1,548,145 A→C N249H (AAC→CAC)  fdnG → formate dehydrogenase‑N, alpha subunit, nitrate‑inducible
RA 1,557,770 A→C L90R (CTC→CGC)  ddpF ← D,D‑dipeptide ABC transporter ATPase
RA 1,627,153 A→C F76L (TTT→TTG dcp ← dipeptidyl carboxypeptidase II
RA 1,638,290 T→G intergenic (‑181/+165) ynfN ← / ← cspI Qin prophage; cold shock‑induced protein/Qin prophage; cold shock protein
RA 1,702,865 T→G I211M (ATT→ATG add → adenosine deaminase
RA 1,704,474 T→G intergenic (‑166/‑77) ydgJ ← / → blr putative oxidoreductase/beta‑lactam resistance membrane protein; divisome‑associated protein
RA 1,736,170 A→C F374C (TTT→TGT)  ydhP ← putative MFS transporter, inner membrane protein
RA 1,740,207 A→C V99V (GTA→GTC ydhC → putative arabinose efflux transporter
RA 1,747,584 A→C S152R (AGC→CGC)  ydhS → putative oxidoreductase
RA 1,784,693 A→C intergenic (+16/+41) fadK → / ← ppsA short chain acyl‑CoA synthetase, anaerobic/phosphoenolpyruvate synthase
RA 1,789,184 T→G D250E (GAT→GAG aroH → 3‑deoxy‑D‑arabino‑heptulosonate‑7‑phosphate synthase, tryptophan repressible
RA 1,804,145 A→C pseudogene (628/1476 nt) arpB → pseudogene, ankyrin repeats
RA 1,804,989 T→G pseudogene (1472/1476 nt) arpB → pseudogene, ankyrin repeats
RA 1,822,544 T→G F29L (TTT→TTG nadE → NAD synthetase, NH3/glutamine‑dependent
RA 1,823,935 A→C N141H (AAC→CAC)  cho → endonuclease of nucleotide excision repair
RA 1,837,673 A→C V154V (GTA→GTC ynjC → putative ABC transporter permease
RA 1,873,762 A→C V63V (GTA→GTC yeaK → aminoacyl‑tRNA editing domain protein
RA 1,880,386 A→C L125R (CTG→CGG)  leuE ← leucine efflux protein
RA 1,880,672 A→C F30V (TTT→GTT)  leuE ← leucine efflux protein
RA 1,898,641 T→G L72R (CTT→CGT)  yoaD → putative membrane‑anchored cyclic‑di‑GMP phosphodiesterase, regulator of cellulose production
RA 1,924,236 A→C L119R (CTG→CGG)  yebZ ← inner membrane protein
MC JC 1,978,503 Δ776 bp insB1insA insB1, insA
RA 1,980,663 T→G E317A (GAA→GCA)  otsA ← trehalose‑6‑phosphate synthase
RA 1,984,547 A→C S175A (TCT→GCT)  araG ← L‑arabinose ABC transporter ATPase
RA 1,988,111 T→G intergenic (‑152/‑111) azuC ← / → yecR acid‑inducible small membrane‑associated protein/lipoprotein, function unknown
RA 1,998,061 T→G Q140P (CAG→CCG)  yecS ← ABC family putative inner membrane permease
RA 2,004,251 A→C K127T (AAA→ACA)  fliD → flagellar filament capping protein
RA 2,025,260 T→G intergenic (+47/‑251) yodD → / → yedP uncharacterized protein/putative mannosyl‑3‑phosphoglycerate phosphatase
RA 2,049,519 A→C K1528Q (AAA→CAA)  yeeJ → putative adhesin
RA 2,056,488 A→C R476R (CGA→CGC amn → AMP nucleosidase
RA 2,059,927 A→C intergenic (+1/+37) asnU → / ← cbl tRNA‑Asn/ssuEADCB/tauABCD operon transcriptional activator
RA 2,083,952 T→G V399V (GTT→GTG sbcB → exodeoxyribonuclease I; exonuclease I
RA 2,103,393 A→C *373G (TGA→GGA)  wbbK ← lipopolysaccharide biosynthesis protein
RA 2,107,808 T→G R174S (AGA→AGC glf ← UDP‑galactopyranose mutase, FAD/NAD(P)‑binding
RA 2,160,092 T→G S569R (AGT→AGG mdtC → multidrug efflux system, subunit C
RA 2,173,363 Δ2 bp intergenic (‑1/+1) gatC ← / ← gatC pseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC/pseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC
RA 2,176,270 A→C S18A (TCA→GCA)  gatZ ← D‑tagatose 1,6‑bisphosphate aldolase 2, subunit
RA 2,187,468 A→C I316R (ATA→AGA)  yehA ← putative fimbrial‑like adhesin protein
RA 2,214,569 T→G K26Q (AAA→CAA)  yehU ← inner membrane putative sensory kinase in two‑component system with YehT
RA 2,217,824 A→C L245R (CTG→CGG)  yehY ← putative ABC transporter permease
RA 2,246,382 T→G I130L (ATC→CTC)  cirA ← colicin IA outer membrane receptor and translocator; ferric iron‑catecholate transporter
RA 2,266,579 T→G I112S (ATT→AGT)  yeiQ → putative NAD‑dependent D‑mannonate oxidoreductase
RA 2,283,668 T→G K94Q (AAA→CAA)  yejK ← spermidine nucleoid‑associated protein
RA 2,316,351 A→C M59L (ATG→CTG)  rcsB → response regulator in two‑component regulatory system with RcsC and YojN
RA 2,338,288 T→G H378P (CAT→CCT)  gyrA ← DNA gyrase (type II topoisomerase), subunit A
RA 2,343,779 A→C S131A (TCC→GCC)  yfaL ← adhesin
RA 2,372,999 T→G F36L (TTT→TTG arnF → undecaprenyl phosphate‑alpha‑L‑ara4N exporter; flippase ArnEF subunit
RA 2,422,240 T→G L178R (CTG→CGG)  yfcH → putative NAD‑dependent nucleotide‑sugar epimerase
RA 2,423,579 T→G intergenic (‑40/+157) yfcI ← / ← hisP transposase_31 family protein/histidine ABC transporter ATPase
RA 2,438,570 A→C P207P (CCA→CCC flk → putative flagella assembly protein
RA 2,486,868 T→G V832G (GTC→GGC)  evgS → hybrid sensory histidine kinase in two‑component regulatory system with EvgA
RA 2,493,412 T→G intergenic (‑158/+355) frc ← / ← yfdX formyl‑CoA transferase, NAD(P)‑binding/uncharacterized protein
RA 2,554,262 T→G Q307H (CAA→CAC eutR ← eut operon transcriptional activator, AraC family
RA 2,560,597 T→G V114G (GTA→GGA)  yffL → CPZ‑55 prophage; uncharacterized protein
RA 2,578,624 A→C intergenic (‑247/‑42) maeB ← / → talA malic enzyme: putative oxidoreductase/phosphotransacetylase/transaldolase A
RA 2,594,162 A→C D566E (GAT→GAG tmcA ← elongator methionine tRNA (ac4C34) acetyltransferase
RA 2,615,883 A→C intergenic (‑2/‑211) yfgO ← / → bepA putative UPF0118 family inner membrane permease/OM protein maintenance and assembly metalloprotease and chaperone, periplasmic
RA 2,617,317 T→G D408E (GAT→GAG bepA → OM protein maintenance and assembly metalloprotease and chaperone, periplasmic
RA 2,626,437 T→G L441L (CTT→CTG ppx → exopolyphosphatase
RA 2,652,948 A→C E152A (GAA→GCA)  sseA → 3‑mercaptopyruvate sulfurtransferase
RA 2,657,657 T→G K427Q (AAA→CAA)  hscA ← DnaK‑like molecular chaperone specific for IscU
RA 2,683,710 A→C Q283P (CAA→CCA)  yphH → putative DNA‑binding transcriptional regulator
RA 2,690,978 T→G S41R (AGC→CGC)  glrK ← sensor protein kinase regulating glmY sRNA in two‑component system with response regulator GlrR
RA 2,724,576 A→C Y391D (TAC→GAC)  kgtP ← alpha‑ketoglutarate transporter
RA 2,731,287 A→C intergenic (‑130/+313) rrsG ← / ← clpB 16S ribosomal RNA of rrnG operon/protein disaggregation chaperone
RA 2,776,304 A→C E129A (GAG→GCG)  yfjX → CP4‑57 prophage; putative antirestriction protein
RA 2,778,122 A→C intergenic (+340/+24) ypjF → / ← ypjA CP4‑57 prophage; toxin of the YpjF‑YfjZ toxin‑antitoxin system/adhesin‑like autotransporter
RA 2,805,674 T→G L287* (TTA→TGA)  proV → glycine betaine/proline ABC transporter periplasmic binding protein
RA 2,814,754 T→G intergenic (‑21/+129) luxS ← / ← gshA S‑ribosylhomocysteine lyase/glutamate‑cysteine ligase
RA 2,822,103 A→C intergenic (‑92/+36) alaS ← / ← recX alanyl‑tRNA synthetase/regulatory protein for RecA
RA 2,849,435 T→G I139L (ATC→CTC)  hycB ← hydrogenase 3, Fe‑S subunit
RA 2,914,828 T→G K58T (AAA→ACA)  rlmD ← 23S rRNA m(5)U1939 methyltransferase, SAM‑dependent
RA 2,964,868 T→G K96N (AAA→AAC thyA ← thymidylate synthetase
RA 2,967,937 A→C Y166* (TAT→TAG ptsP ← PEP‑protein phosphotransferase enzyme I; GAF domain containing protein
RA 2,993,609 T→G intergenic (+139/‑29) ygeH → / → ygeI putative transcriptional regulator/uncharacterized protein
RA 3,105,735 A→C K8Q (AAA→CAA)  nupG → nucleoside transporter
RA 3,107,858 A→C L433R (CTG→CGG)  speC ← ornithine decarboxylase, constitutive
RA 3,112,162 A→C pseudogene (780/849 nt) yghF ← pseudogene, secretion pathway protein, C‑type protein homology;putative transport; Not classified; putative general secretion pathway for protein export (GSP)
RA 3,120,887 T→G G131G (GGA→GGC glcA ← glycolate transporter
RA 3,140,222 T→G I33L (ATT→CTT)  hybF ← protein involved with the maturation of hydrogenases 1 and 2
RA 3,149,618 A→C intergenic (‑147/‑44) yqhA ← / → yghA UPF0114 family putative inner membrane protein/putative oxidoreductase
RA 3,186,190 A→C I2L (ATT→CTT)  insC1 → IS2 repressor TnpA
RA 3,204,192 T→G intergenic (‑61/+29) bacA ← / ← folB undecaprenyl pyrophosphate phosphatase/dihydroneopterin aldolase and dihydroneopterin triphosphate 2'‑epimerase
RA 3,263,574 T→G V29V (GTA→GTC tdcD ← propionate kinase/acetate kinase C, anaerobic
RA 3,286,411 A→C N48H (AAT→CAT)  agaI → galactosamine‑6‑phosphate isomerase
RA 3,286,675 A→C N136H (AAC→CAC)  agaI → galactosamine‑6‑phosphate isomerase
RA 3,308,757 T→G L56F (TTA→TTC nlpI ← lipoprotein involved in osmotic sensitivity and filamentation
RA 3,317,296 A→C L77L (CTT→CTG nusA ← transcription termination/antitermination L factor
RA 3,334,208 A→C I167L (ATC→CTC)  ispB → octaprenyl diphosphate synthase
RA 3,343,061 T→G S76A (TCG→GCG)  lptC → periplasmic membrane‑anchored LPS‑binding protein; LPS export protein
RA 3,362,192 A→C S27S (TCA→TCC yhcA → putative periplasmic chaperone protein
RA 3,365,608 T→G intergenic (+57/+94) yhcE → / ← insH1 pseudogene fragment; interrupted by IS5/IS5 transposase and trans‑activator
RA 3,372,680 A→C intergenic (‑106/+3) nanT ← / ← nanA sialic acid transporter/N‑acetylneuraminate lyase
RA 3,373,852 T→G E213A (GAA→GCA)  nanR ← sialic acid‑inducible nan operon repressor
RA 3,398,511 T→G E122A (GAG→GCG)  mreD ← cell wall structural complex MreBCD transmembrane component MreD
RA 3,400,678 T→G E137A (GAA→GCA)  mreB ← cell wall structural complex MreBCD, actin‑like component MreB
RA 3,448,548 A→C T7T (ACT→ACG rpsQ ← 30S ribosomal subunit protein S17
RA 3,476,689 T→G E244A (GAG→GCG)  fkpA ← FKBP‑type peptidyl‑prolyl cis‑trans isomerase (rotamase)
RA 3,484,500 A→C K4T (AAA→ACA)  prkB → putative phosphoribulokinase
RA 3,541,448 A→C N348H (AAC→CAC)  feoB → ferrous iron transporter protein B and GTP‑binding protein; membrane protein
RA 3,549,583 T→G D163A (GAT→GCT)  malQ ← 4‑alpha‑glucanotransferase (amylomaltase)
RA 3,554,067 T→G L328R (CTG→CGG)  malT → mal regulon transcriptional activator
RA 3,559,217 A→C D317A (GAT→GCT)  rtcR → sigma 54‑dependent transcriptional regulator of rtcBA expression
RA 3,560,455 +G intergenic (‑2/+1) glpR ← / ← glpR pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon/pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon
RA 3,635,992 T→G intergenic (+99/+216) yhiM → / ← yhiN acid resistance protein, inner membrane/putative oxidoreductase
RA 3,657,161 T→G L56* (TTA→TGA)  hdeD → acid‑resistance membrane protein
RA 3,659,521 T→G L97* (TTA→TGA)  mdtE → anaerobic multidrug efflux transporter, ArcA‑regulated
RA 3,671,117 A→C E509A (GAA→GCA)  treF → cytoplasmic trehalase
RA 3,681,475 T→G I156L (ATC→CTC)  yhjJ ← putative periplasmic M16 family chaperone
RA 3,683,667 A→C I651S (ATT→AGT)  yhjK ← cyclic‑di‑GMP phosphodiesterase
RA 3,791,001 A→C V115V (GTT→GTG tdh ← L‑threonine 3‑dehydrogenase, NAD(P)‑binding
RA 3,814,738 A→C K82T (AAA→ACA)  slmA → nucleoid occlusion factor, anti‑FtsZ division inhibitor
RA 3,824,507 A→C *703S (TAA→TCA)  spoT → bifunctional (p)ppGpp synthetase II/ guanosine‑3',5'‑bis pyrophosphate 3'‑pyrophosphohydrolase
RA 3,833,231 A→C M436R (ATG→AGG)  yicI ← putative alpha‑glucosidase
RA 3,864,379 A→C I161L (ATT→CTT)  yidP → UTRA domain‑containing GntR family putative transcriptional regulator
RA 3,882,059 A→C I88S (ATT→AGT)  dnaN ← DNA polymerase III, beta subunit
RA 3,898,162 T→G Y47* (TAT→TAG cbrC → UPF0167 family protein
RA 3,941,081 A→C W83G (TGG→GGG)  yieP ← putative transcriptional regulator
RA 3,972,742 A→C Q74P (CAG→CCG)  rffG → dTDP‑glucose 4,6‑dehydratase
RA 3,972,815 T→G I98M (ATT→ATG rffG → dTDP‑glucose 4,6‑dehydratase
RA 3,981,539 T→G L218R (CTG→CGG)  yifK → putative APC family amino acid transporter
RA 4,004,063 A→C L39R (CTG→CGG)  rarD ← putative chloramphenical resistance permease
RA 4,029,177 T→G K599Q (AAG→CAG)  fadB ← fused 3‑hydroxybutyryl‑CoA epimerase/delta(3)‑cis‑delta(2)‑trans‑enoyl‑CoA isomerase/enoyl‑CoA hydratase/3‑hydroxyacyl‑CoA dehydrogenase
RA 4,034,898 T→G R97R (CGT→CGG hemG → protoporphyrin oxidase, flavoprotein
RA 4,055,097 T→G D61A (GAC→GCC)  glnG ← fused DNA‑binding response regulator in two‑component regulatory system with GlnL: response regulator/sigma54 interaction protein
RA 4,073,504 T→G Q23P (CAA→CCA)  yihU ← 3‑sulpholactaldehyde (SLA) reductase, NADH‑dependent; gamma‑hydroxybutyrate dehydrogenase, NADH‑dependent
RA 4,073,554 A→C F6L (TTT→TTG yihU ← 3‑sulpholactaldehyde (SLA) reductase, NADH‑dependent; gamma‑hydroxybutyrate dehydrogenase, NADH‑dependent
RA 4,084,783 A→C F347C (TTC→TGC)  fdoG ← formate dehydrogenase‑O, large subunit
RA 4,093,592 T→G L227F (TTA→TTC rhaD ← rhamnulose‑1‑phosphate aldolase
RA 4,097,080 A→C I123M (ATT→ATG rhaB ← rhamnulokinase
RA 4,101,056 A→C S83R (AGC→CGC)  sodA → superoxide dismutase, Mn
RA 4,116,511 T→G I238L (ATT→CTT)  glpK ← glycerol kinase
RA 4,146,701 A→C S148S (TCA→TCC pflC → putative [formate‑C‑acetyltransferase 2]‑activating enzyme; pyruvate formate‑lyase 1‑activating enzyme
RA 4,149,302 A→C Y322D (TAT→GAT)  eptC ← LPS heptose I phosphoethanolamine transferase
RA 4,173,777 T→G N232K (AAT→AAG birA → bifunctional biotin‑[acetylCoA carboxylase] holoenzyme synthetase/ DNA‑binding transcriptional repressor, bio‑5'‑AMP‑binding
RA 4,179,038 A→C K54Q (AAA→CAA)  rplA → 50S ribosomal subunit protein L1
RA 4,179,175 T→G D99E (GAT→GAG rplA → 50S ribosomal subunit protein L1
RA 4,182,877 T→G F545V (TTC→GTC)  rpoB → RNA polymerase, beta subunit
RA 4,187,714 A→C K789Q (AAA→CAA)  rpoC → RNA polymerase, beta prime subunit
RA 4,220,666 T→G K608Q (AAA→CAA)  arpA ← ankyrin repeat protein
RA 4,268,001 T→G A296A (GCT→GCG tyrB → tyrosine aminotransferase, tyrosine‑repressible, PLP‑dependent
RA 4,284,935 A→C L93L (CTT→CTG yjcH ← DUF485 family inner membrane protein
RA 4,285,155 T→G E20A (GAA→GCA)  yjcH ← DUF485 family inner membrane protein
RA 4,285,977 T→G V465V (GTA→GTC acs ← acetyl‑CoA synthetase
RA 4,296,381 +GC intergenic (+587/+55) gltP → / ← yjcO glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein
RA 4,299,529 A→C intergenic (‑163/+35) fdhF ← / ← mdtP formate dehydrogenase‑H, selenopolypeptide subunit/outer membrane factor of efflux pump
RA 4,310,903 T→G L26F (TTA→TTC alsA ← D‑allose ABC transporter ATPase
RA 4,313,825 A→C intergenic (+26/‑43) rpiB → / → yjdP ribose 5‑phosphate isomerase B/allose 6‑phosphate isomerase/putative periplasmic protein
RA 4,335,035 T→G N186H (AAC→CAC)  eptA ← lipid A phosphoethanolamine transferase
RA 4,351,371 T→G I98L (ATT→CTT)  dcuS ← sensory histidine kinase in two‑component regulatory system with DcuR, regulator of anaerobic fumarate respiration
RA 4,399,742 A→C D164A (GAT→GCT)  miaA → delta(2)‑isopentenylpyrophosphate tRNA‑adenosine transferase
RA 4,424,756 A→C L12L (CTT→CTG yjfY ← YhcN family protein, periplasmic
RA 4,445,958 A→C Q616P (CAA→CCA)  tamB → translocation and assembly module for autotransporter export, inner membrane subunit
RA 4,510,292 A→C intergenic (+159/+398) yjhV → / ← fecE pseudogene, KpLE2 phage‑like element/ferric citrate ABC transporter ATPase
RA 4,525,988 A→C intergenic (‑185/+118) yjhI ← / ← sgcR putative DNA‑binding transcriptional regulator/putative DNA‑binding transcriptional regulator
RA 4,530,170 A→C F25C (TTC→TGC)  sgcC ← putative PTS system EIIC permease component
RA 4,543,958 T→G V77V (GTT→GTG fimI → fimbrial protein involved in type 1 pilus biosynthesis
RA 4,560,365 A→C L106V (TTA→GTA)  yjiH ← nucleoside recognition pore and gate family putative inner membrane transporter
RA 4,563,842 A→C intergenic (‑239/+80) yjiK ← / ← yjiL SdiA‑regulated family putative membrane‑anchored protein; putative phytase‑like esterase/putative ATPase, activator of (R)‑hydroxyglutaryl‑CoA dehydratase
RA 4,568,563 A→C intergenic (‑44/‑435) mdtM ← / → yjiP multidrug efflux system protein/pseudogene, transposase_31 family protein
RA 4,594,311 T→G I138L (ATC→CTC)  lgoT ← putative L‑galactonate:H+ symporter
RA 4,598,247 T→G N399H (AAC→CAC)  opgB ← OPG periplasmic biosynthetic phosphoglycerol transferases I (membrane‑bound) and II (soluble)
RA 4,615,781 T→G I429L (ATC→CTC)  yjjI ← DUF3029 family protein, putative glycine radical enzyme

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 3423729–3424234 3424560–3424238 5–832 30 [29] [29] 31 [rrfD]–[rrlD] [rrfD], [rrlD]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 1207790 =4 (0.070)47 (0.880) 41/266 0.5 93.5% coding (290/630 nt) stfP e14 prophage; uncharacterized protein
?NC_000913 1209619 = 3 (0.060)pseudogene (1/501 nt) stfE pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related
* ? NC_000913 = 12078054 (0.070)29 (0.540) 28/266 1.9 89.8% coding (305/630 nt) stfP e14 prophage; uncharacterized protein
?NC_000913 = 1209602 3 (0.060)pseudogene (18/501 nt) stfE pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related
* ? NC_000913 = 12994980 (0.000)61 (1.050) 55/290 0.2 100% intergenic (+253/‑1684) ychE/oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein
?NC_000913 1300698 = 0 (0.000)intergenic (+1453/‑484) ychE/oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein