breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Marginal read alignment evidence (highest frequency 20 of 143 shown, sorted by frequency from high to low)
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP009273766,3530TG61.5% 5.3 / 12.6 13intergenic (+286/‑561)mngB/cydAmannosylglycerate hydrolase/cytochrome ubiquinol oxidase subunit I
*NZ_CP009273964,1420TG59.3% 14.6 / 22.9 27G107G (GGT→GGGlpxKtetraacyldisaccharide 4'‑kinase
*NZ_CP009273822,9550TG57.7% 15.4 / 30.8 26T495P (ACC→CCC) ybhFATP‑binding cassette domain‑containing protein
*NZ_CP009273822,9630TG57.1% 3.5 / 35.0 28D492A (GAC→GCC) ybhFATP‑binding cassette domain‑containing protein
*NZ_CP0092731,500,2950AC56.5% 22.7 / 30.2 23F15V (TTT→GTT) ydcOBenE family transporter YdcO
*NZ_CP0092731,371,3970AC56.2% 33.4 / 43.6 32K103N (AAA→AACycjSGfo/Idh/MocA family oxidoreductase
*NZ_CP0092731,500,2850AC56.0% 24.2 / 32.0 25V18G (GTA→GGA) ydcOBenE family transporter YdcO
*NZ_CP009273353,6010CA55.2% 12.3 / 30.4 29L267F (TTG→TTTcynRtranscriptional regulator CynR
*NZ_CP0092732,888,4170AC54.5% 8.2 / 11.6 11V241G (GTA→GGA) ygcQelectron transfer flavoprotein subunit alpha/FixB family protein
*NZ_CP0092733,029,1160AC54.2% 21.6 / 32.8 24G175G (GGT→GGGprfBpeptide chain release factor 2
*NZ_CP0092734,558,3210TG53.7% 70.0 / 98.0 69T6P (ACC→CCC) mdtMmultidrug efflux MFS transporter MdtM
*NZ_CP009273353,5860AT53.3% 8.4 / 42.0 30N272K (AAT→AAAcynRtranscriptional regulator CynR
*NZ_CP0092732,380,3410GA53.3% 3.7 / 19.6 15intergenic (+31/‑72)yfbL/yfbMM28 family metallopeptidase/YfbM family protein
*NZ_CP0092731,350,4580TG52.4% 15.3 / 26.1 21T304P (ACC→CCC) sapApeptide ABC transporter substrate‑binding protein SapA
*NZ_CP0092733,273,8300AC52.4% 19.5 / 28.1 21T85T (ACA→ACCagaVPTS N‑acetylgalactosamine transporter subunit IIB
*NZ_CP0092733,781,3850TG51.3% 43.8 / 56.7 39G396G (GGT→GGGenvCmurein hydrolase activator EnvC
*NZ_CP009273595,0320TG50.0% 15.6 / 16.9 26V288G (GTG→GGG) cusACu(+)/Ag(+) efflux RND transporter permease subunit CusA
*NZ_CP009273172,7550AG49.0% 47.8 / 15.6 52T388A (ACG→GCG) clcAH(+)/Cl(‑) exchange transporter ClcA
*NZ_CP009273645,9200AT48.6% 36.7 / 20.2 37R9R (CGT→CGAcitEcitrate (pro‑3S)‑lyase subunit beta
*NZ_CP009273937,1690TG48.6% 34.3 / 18.8 38V252G (GTG→GGG) dmsAdimethylsulfoxide reductase subunit A

Marginal new junction evidence (sorted from low to high skew)
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NZ_CP009273 = 120326151 (1.020)5 (0.120) 4/134 2.9 13.5% coding (305/630 nt) BW25113_RS05990 hypothetical protein
?NZ_CP009273 = 1205058 23 (0.570)coding (57/540 nt) BW25113_RS06005 phage tail protein