breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
RA 49,791 G→A intergenic (+160/‑32) kefC → / → folA glutathione‑regulated potassium‑efflux system protein KefC/type 3 dihydrofolate reductase
RA 49,910 T→G W30G (TGG→GGG)  folA → type 3 dihydrofolate reductase
MC JC 2,399,465 Δ7 bp coding (650‑656/939 nt) lrhA ← transcriptional regulator LrhA
RA 3,631,618 Δ1 bp coding (617/1500 nt) pitA → inorganic phosphate transporter PitA

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NZ_CP009273 356981 357567 587 4 [0] [0] 3 lacA galactoside O‑acetyltransferase
* * ÷ NZ_CP009273 578962 579047 86 2 [1] [1] 2 BW25113_RS26085/appY tail fiber assembly protein/DNA‑binding transcriptional activator AppY
* * ÷ NZ_CP009273 579534 579626 93 2 [1] [1] 2 appY DNA‑binding transcriptional activator AppY
* * ÷ NZ_CP009273 748380 748417 38 2 [1] [0] 4 ybgD/gltA fimbrial protein/citrate synthase
* * ÷ NZ_CP009273 1098967 1098984 18 2 [1] [1] 2 csgD/csgB biofilm master transcriptional regulator CsgD/curli minor subunit CsgB
* * ÷ NZ_CP009273 1206656 1206676 21 3 [1] [1] 3 mcrA/BW25113_RS23640 5‑methylcytosine‑specific endonuclease McrA/isocitrate/isopropylmalate family dehydrogenase
* * ÷ NZ_CP009273 1207571 1207655 85 2 [1] [1] 2 iraM/BW25113_RS25985 anti‑adapter protein IraM/protein YmgK
* * ÷ NZ_CP009273 1211111 1211133 23 2 [1] [0] 2 bluF/ycgZ blue light‑responsive regulator BluF/regulatory protein YcgZ
* * ÷ NZ_CP009273 1362258 1362268 11 2 [1] [0] 2 pspF/pspA phage shock protein operon transcriptional activator/phage shock protein PspA
* * ÷ NZ_CP009273 1459463 1459480 18 2 [1] [1] 2 BW25113_RS25580 hypothetical protein
* * ÷ NZ_CP009273 2768281 2768282 2 4 [1] [0] 2 BW25113_RS13770 hypothetical protein
* * ÷ NZ_CP009273 2984559 2984593 35 4 [1] [1] 2 BW25113_RS14825/ygeG hypothetical protein/tetratricopeptide repeat protein
* * ÷ NZ_CP009273 3354469 3354482 14 3 [1] [1] 2 gltD/gltF glutamate synthase subunit GltD/DUF1120 domain‑containing protein
* * ÷ NZ_CP009273 4466835 4466874 40 2 [1] [1] 2 BW25113_RS22080 hypothetical protein

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NZ_CP009273 360104 =NA (NA)74 (2.710) 33/168 0.0 NA intergenic (‑35/+251) lacZ/BW25113_RS23320 beta‑galactosidase/relaxase/mobilization nuclease domain‑containing protein
?NZ_CP009273 = 360286 NA (NA)intergenic (‑217/+69) lacZ/BW25113_RS23320 beta‑galactosidase/relaxase/mobilization nuclease domain‑containing protein
* ? NZ_CP009273 4532499 =3 (0.110)13 (0.530) 7/150 1.1 56.9% intergenic (+49/‑433) fimE/fimA type 1 fimbria switch DNA invertase FimE/type 1 fimbrial major subunit FimA
?NZ_CP009273 4532813 = 17 (0.700)intergenic (+363/‑119) fimE/fimA type 1 fimbria switch DNA invertase FimE/type 1 fimbrial major subunit FimA