breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Marginal read alignment evidence (highest frequency 20 of 280 shown, sorted by frequency from high to low)
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0092731,965,7820TG74.2% 63.6 / 19.6 31E389D (GAA→GACtarmethyl‑accepting chemotaxis protein II
*NZ_CP0092731,906,0110TG73.9% ‑3.4 / 11.8 23T275P (ACC→CCC) htpXprotease HtpX
*NZ_CP0092731,276,2490G.73.3% 22.6 / 13.3 15coding (930/3744 nt)narGnitrate reductase subunit alpha
*NZ_CP0092734,064,5640AG73.1% 33.0 / 17.0 26Y185H (TAT→CAT) yihSsulfoquinovose isomerase
*NZ_CP0092731,903,0610AT72.7% 37.0 / 14.4 22intergenic (‑38/‑121)mgrB/yobHPhoP/PhoQ regulator MgrB/YobH family protein
*NZ_CP0092732,283,8710T.70.6% 17.8 / 18.7 17intergenic (‑347/‑108)BW25113_RS11435/narPIS5‑like element IS5 family transposase/nitrate/nitrite response regulator protein NarP
*NZ_CP0092732,753,1690TC70.6% 16.6 / 11.1 19S276P (TCA→CCA) yfjIYfjI family protein
*NZ_CP0092734,073,1630AT69.8% 84.0 / 46.7 53D26V (GAT→GTT) BW25113_RS20185hypothetical protein
*NZ_CP0092731,503,9650TC69.2% 28.0 / 20.5 26D67D (GAT→GAChicBtype II toxin‑antitoxin system antitoxin HicB
*NZ_CP0092732,439,1010TC69.2% 28.7 / 20.8 26K255E (AAG→GAG) mepApenicillin‑insensitive murein endopeptidase
*NZ_CP0092731,558,4390AG68.0% 27.2 / 19.9 25Y518H (TAT→CAT) dosPoxygen‑sensing cyclic‑di‑GMP phosphodiesterase
*NZ_CP0092733,579,1680TC67.3% 54.4 / 45.9 49E339G (GAA→GGA) ggtgamma‑glutamyltransferase
*NZ_CP009273539,9750AG66.7% 23.1 / 22.6 27D258D (GAT→GACallCallantoate deiminase
*NZ_CP009273646,2650TG66.7% ‑0.2 / 27.1 30T350P (ACC→CCC) citC[citrate (pro‑3S)‑lyase] ligase
*NZ_CP0092732,982,5550TC66.7% 35.7 / 26.3 33F232S (TTC→TCC) yqeItranscriptional regulator
*NZ_CP0092734,084,6530AG66.7% 14.3 / 18.6 32G358G (GGT→GGCfrvBPTS fructose‑like transporter subunit IIBC
*NZ_CP0092734,153,8160TC66.7% 39.9 / 18.7 33S84P (TCA→CCA) btuBTonB‑dependent vitamin B12 receptor BtuB
*NZ_CP0092731,980,4200AG65.6% 32.3 / 27.7 32G5G (GGA→GGGftnBnon‑heme ferritin‑like protein
*NZ_CP0092731,906,0070TG65.2% 8.1 / 15.1 23H276P (CAC→CCC) htpXprotease HtpX
*NZ_CP0092732,526,0500AG64.9% 36.0 / 33.9 37T95A (ACC→GCC) cysKcysteine synthase A

Marginal new junction evidence (lowest skew 10 of 15 shown)
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NZ_CP009273 4049856 =39 (1.100)5 (0.160) 5/148 2.1 14.4% intergenic (‑157/+129) glnL/glnA nitrogen regulation protein NR(II)/glutamate‑‑ammonia ligase
?NZ_CP009273 4049890 = 25 (0.800)intergenic (‑191/+95) glnL/glnA nitrogen regulation protein NR(II)/glutamate‑‑ammonia ligase
* ? NZ_CP009273 2248837 =32 (0.900)5 (0.140) 5/168 2.4 13.5% coding (43/699 nt) yeiL transcriptional regulator YeiL
?NZ_CP009273 3576788 = NA (NA)coding (391/417 nt) BW25113_RS17825 YrhA family protein
* ? NZ_CP009273 1040657 =20 (0.570)4 (0.130) 4/150 2.4 15.1% coding (603/1140 nt) gfcE polysaccharide export protein
?NZ_CP009273 1040766 = 27 (0.850)coding (494/1140 nt) gfcE polysaccharide export protein
* ? NZ_CP009273 442673 =25 (0.710)5 (0.160) 4/146 2.4 18.3% coding (489/891 nt) cyoE heme o synthase
?NZ_CP009273 442734 = 23 (0.750)coding (428/891 nt) cyoE heme o synthase
* ? NZ_CP009273 3920778 =58 (1.640)3 (0.100) 3/144 2.7 6.1% coding (264/993 nt) asnA aspartate‑‑ammonia ligase
?NZ_CP009273 3920817 = 42 (1.380)coding (303/993 nt) asnA aspartate‑‑ammonia ligase
* ? NZ_CP009273 215975 =42 (1.190)3 (0.100) 3/146 2.7 8.6% coding (107/708 nt) tsaA tRNA (N6‑threonylcarbamoyladenosine(37)‑N6)‑methyltransferase TrmO
?NZ_CP009273 216027 = 27 (0.880)coding (55/708 nt) tsaA tRNA (N6‑threonylcarbamoyladenosine(37)‑N6)‑methyltransferase TrmO
* ? NZ_CP009273 = 453250718 (0.510)3 (0.090) 3/150 2.8 8.9% intergenic (+57/‑425) fimE/fimA type 1 fimbria switch DNA invertase FimE/type 1 fimbrial major subunit FimA
?NZ_CP009273 = 4532803 45 (1.420)intergenic (+353/‑129) fimE/fimA type 1 fimbria switch DNA invertase FimE/type 1 fimbrial major subunit FimA
* ? NZ_CP009273 3757365 =13 (0.370)3 (0.090) 3/152 2.8 12.3% coding (1823/4134 nt) rhsA RHS element protein RhsA
?NZ_CP009273 3757417 = 31 (0.970)coding (1875/4134 nt) rhsA RHS element protein RhsA
* ? NZ_CP009273 = 293695939 (1.100)4 (0.120) 3/152 2.8 11.4% coding (264/1206 nt) csdA cysteine desulfurase CsdA
?NZ_CP009273 = 2936998 27 (0.840)coding (303/1206 nt) csdA cysteine desulfurase CsdA
* ? NZ_CP009273 = 187038028 (0.790)3 (0.090) 3/150 2.8 10.7% coding (451/1182 nt) nimT 2‑nitroimidazole transporter
?NZ_CP009273 = 1870397 25 (0.790)coding (468/1182 nt) nimT 2‑nitroimidazole transporter