Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
MC JC	CP009273	4,303,167	Δ450 bp	100%	coding (1‑450/450 nt)	rpiB →	ribose 5‑phosphate isomerase B/allose 6‑phosphate isomerase

Missing coverage evidence...
			seq id	start	end	size	←reads	reads→	gene	description
*	*	÷	CP009273	4303167	4303616	450	60 [0]	[0] 60	rpiB	ribose 5‑phosphate isomerase B/allose 6‑phosphate isomerase

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	CP009273	= 4303166	0 (0.000)	55 (0.980)	52/270	NT	100%	intergenic (‑358/‑1)	alsR/rpiB	d‑allose‑inducible als operon transcriptional repressor; autorepressor; repressor of rpiR/ribose 5‑phosphate isomerase B/allose 6‑phosphate isomerase
	?	CP009273	4303617 =	0 (0.000)					intergenic (+1/‑68)	rpiB/yjdP	ribose 5‑phosphate isomerase B/allose 6‑phosphate isomerase/putative periplasmic protein

TGGTGAGTGTCTTTAACGCTATTGCGCTGTCAGATGTAACTCTGTAAAACAGATCAGGAAGGCGTAATTCATCGATTTTGGGTGTTTTTAATTCATTAACATCACAAATGTTTTTTGATTGTGAAGTTTTGCACGGACGGGGAAGATGAATGAAAAAGATTGCATTTGGCTGTGATCATGTCGGTTTCATTTTAAAACATGAAATAGTGGCACATTTAGTTGAGCGTGGCGTTGAAGTGATTGATAAAGGAACCTGGTCGTCAGAGCGTACTGATTATCCACATTACGCCAGTCAAG  >  CP009273/4303018‑4303314                                                                                                                                                     |                                                                                                                                                     tGGTGAGTGTCTTTAACGCTATTGCGCTGTCAGATGTAACTCTGTAAAACAGATCAGGAAGGCGTAATTCATCGATTTTGGGTGTTTTTAATTCATTAACATCACAAATGTTTTTTGATTGTGAAGTTTTGCACGGACGGGGAAGATGAg                                                                                                                                                     <  2:125998‑M1/150‑2 (MQ=255)   gTGAGTGTCTTTAACGCTATTGCGCTGTCAGATGTAACTCTGTAAAACAGATCAGGAAGGCGTAATTCATCGATTTTGGGTGTTTTTAATTCATTAACATCACAAATGTTTTTTGATTGTGAAGTTTTGCACGGACGGGGAAGATGAgat                                                                                                                                                   <  1:967193‑M1/150‑4 (MQ=255)        tgtCTTTAACGCTATTGCGCTGTCAGATGTAACTCTGTAAAACAGATCATGAAGGCGTATTTCATCGATTTGGGGCGTTTTTAATTCATTAACATCACAAATGTTTTTTGATTGTGAAGTTTTGCACGGACGTGGAAGCTGTgattcatc                                                                                                                                              >  2:354284‑M1/1‑142 (MQ=255)        tgtCTTTAACGCTATTGCGCTGTCAGATGTAACTCTGTAAAACAGATCAGGAAGGCGTAATTCATCGATTTTGGGTGTTTTTAATTCATTAACATCACAAATGTTTTTTGATTGTGAAGTTTTGCACGGACGGGGAAGATGAgattcatc                                                                                                                                              >  1:202926‑M1/1‑142 (MQ=255)         gtCTTTAACGCTATTGCGCTGTCAGATGTAACTCTGTAAAACAGATCAGGAAGGCGTAATTCATCGATTTTGGGTGTTTTTAATTCATTAACATCACAAATGTTTTTTGATTGTGAAGTTTTGCACGGACGGGGAAGATGAgattcatc                                                                                                                                              >  1:386561‑M1/1‑141 (MQ=255)           cTTTAACGCTATTGCGCTGTCAGATGTAACTCTGTAAAACAGATCAGGAAGGCGTAATTCATCGATTTTGGGTGTTTTTAATTCATTTACATCACAAATGTTTTTTGATTGTGAAGTTTTGCACGGACGGGGAAGATGAgattcatccac                                                                                                                                           >  2:511482‑M1/1‑139 (MQ=255)              tAACGCTATTGCGCTGTCAGATGTAACTCTGTAAAACAGATCAGGAAGGCGTAATTCATCGATTTTGGGTGTTTTTAATTCATTAACATCACAAATGTTTTTTGATTGTGAAGTTTTGCACGGACGGGGAAGATGAgattcatccactac                                                                                                                                        >  1:508287‑M1/1‑136 (MQ=255)               aaCGCTATTGCGCTGTCAGATGTAACTCTGTAAAACAGATCAGGAAGGCGTAATTCATCGATTTTGGGTGTTTTTAATTCATTAACATCACAAATGTTTTTTGATTGTGAAGTTTTGCACGGACGGGGAAGATGAgattcatccactact                                                                                                                                       >  1:428941‑M1/1‑135 (MQ=255)                    tATTGCGCTGTCAGATGTAACTCTGTAAAACAGATCAGGAAGGCGTAATTCATCGATTTTGGGTGTTTTTAATTCATTAACATCACAAATGTTTTTTGATTGTGAAGTTTTGCACGGACGGGGAAGATGAgattcatccactacttgcat                                                                                                                                  >  1:490587‑M1/1‑130 (MQ=255)                      ttGCGCTGTCAGATGTAACTCTGTAAAACAGATCAGGAAGGCGTAATTCATCGATTTTGGGTGTTTTTAATTCATTAACATCACAAATGTTTTTTGATTGTGAAGTTTTGCACGGACGGGGAAGATGAgattcatccactacttgcat                                                                                                                                  <  1:78383‑M1/148‑21 (MQ=255)                         cgcTGTCAGATGTAACTCTGTAAAACAGATCAGGAAGGCGTAATTCATCGATTTTGGGTGTTTTTAATTCATTAACATCACAAATGTTTTTTGATTGTGAAGTTTTGCACGGACGGGGAAGATGAgattcatccactacttgcatggat                                                                                                                              <  2:664846‑M1/149‑25 (MQ=255)                          gcTGTCAGATGTAACTCTGTAAAACAGATCAGGAAGGCGTAATTCATCGATTTTGGGTGTTTTTAATTCATTAACATCACAAATGTTTTTTGATTGTGAAGTTTTGCACGGACGGGGAAGATGAgattcatccactacttgcatggat                                                                                                                              >  1:56019‑M1/1‑124 (MQ=255)                           cTGTCAGATGTAACTCTGTAAAACAGATCAGGAAGGCGTAATTCATCGATTTTGGGTGTTTTTAATTCATTAACATCACAAATGTTTTTTGATTGTGAAGTTTTGCACGGACGGGGAAGATGAgattcatccactacttgcatggatgag                                                                                                                           <  1:712263‑M1/150‑28 (MQ=255)                            tGTCAGATGTAACTCTGTAAAACAGATCAGGAAGGCGTAATTCATCGATTTTGGGTGTTTTTAATTCATTAACATCACAAATGTTTTTTGATTGTGAAGTTTTGCACGGACGGGGAAGATGAgattcatccactacttgcatggatgag                                                                                                                           >  2:418553‑M1/1‑122 (MQ=255)                            tGTCAGATGTAACTCTGTAAAACAGATCAGGAAGGCGTAATTCATCGATTTTGGGTGTTTTTAATTCATTAACATCACAAATGTTTTTTGATTGTGAAGTTTTGCACGGACGGGGAAGATGAgattcatccactacttgcatggatgag                                                                                                                           >  1:27568‑M1/1‑122 (MQ=255)                             gTCAGATGTAACTCTGTAAAACAGATCAGGAAGGCGTAATTCATCGATTTTGGGTGTTTTTAATTCATTAACATCACAAATGTTTTTTGATTGTGAAGTTTTGCACGGACGGGGAAGATGAgattcatccactacttgcatggatgagt                                                                                                                          >  2:414945‑M1/1‑121 (MQ=255)                             gTCAGATGTAACTCTGTAAAACAGATCAGGAAGGCGTAATTCATCGATTTTGGGTGTTTTTAATTCATTAACATCACAAATGTTTTTTGATTGTGAAGTTTTGCACGGACGGGGAAGATGAgattcatccactacttgcatggatgagt                                                                                                                          >  1:859457‑M1/1‑121 (MQ=255)                              tCAGATGTAACTCTGTAAAACAGATCAGGAAGGCGTAATTCATCGATTTTGGGTGTTTTTAATTCATTAACATCACAAATGTTTTTTGATTGTGAAGTTTTGCACGGACGGGGAAGATGAgattcatccactacttgcatggatgagta                                                                                                                         <  2:214438‑M1/149‑30 (MQ=255)                                  aTGTAACTCTGTAAAACAGATCAGGAAGGCGTAATTCATCGATTTTGGGTGTTTTTAATTCATTAACATCACAAATGTTTTTTGATTGTGAAGTTTTGCACGGACGGGGAAGATGAgattcatccactacttgcatggatgagtaatgat                                                                                                                    <  2:944112‑M1/150‑35 (MQ=255)                                     tAACTCTGTAAAACAGATCAGGAAGGCGTAATTCATCGATTTTGGGTGTTTTTAATTCATTAACATCACAAATGTTTTTTGATTGTGAAGTTTTGCACGGACGGGGAAGATGAgattcatccactacttgcatggatgagtaatgattaa                                                                                                                 >  1:212306‑M1/1‑113 (MQ=255)                                           tGTAAAACAGATCAGGAAGGCGTAATTCATCGATTTTGGGTGTTTTTAATTCATTAACATCACAAATGTTTTTTGATTGTGAAGTTTTGCACGGACGGGGAAGATGAgattcatccactacttgcatggatgagtaatgattaatgtgga                                                                                                           >  1:548507‑M1/1‑107 (MQ=255)                                            gTAAAACAGATCAGGAAGGCGTAATTCATCGATTTTGGGTGTTTTTAATTCATTAACATCACAAATGTTTTTTGATTGTGAAGTTTTGCACGGACGGGGAAGATGAgattcatccactacttgcatggatgagtaatgattaatgtgga                                                                                                           >  1:688349‑M1/1‑106 (MQ=255)                                             tAAAACAGATCAGGAAGGCGTAATTCATCGATTTTGGGTGTTTTTAATTCATTAACATCACAAATGTTTTTTGATTGTGAAGTTTTGCACGGACGGGGAAGATGAgattcatccactacttgcatggatgagtaatgattaatgtggata                                                                                                         <  1:67512‑M1/150‑46 (MQ=255)                                             tAAAACAGATCAGGAAGGCGTAATTCATCGATTTTGGGTGTTTTTAATTCATTAACATCACAAATGTTTTTTGATTGTGAAGTTTTGCACGGACGGGGAAGATGAgattcatccactacttgcatggatgagtaatgattaatgtgg                                                                                                            <  2:217756‑M1/147‑43 (MQ=255)                                                        aGGAAGGCGTAATTCATCGATTTTGGGTGTTTTTAATTCATTAACATCACAAATGTTTTTTGATTGTGAAGTTTTGCACGGACGGGGAAGATGAgattcatccactacttgcatggatgagtaatgattaatgtggatagagtttctttt                                                                                              >  1:987366‑M1/1‑94 (MQ=255)                                                        aGGAAGGCGTAATTAATCGATTTTGGGTGTTTTTAATTCATTAACATCACAAATGTTTTTTGATTGTGAAGTTTTGCACGGACGGGGAAGATGAgattcatccactacttgcatggatgagtaatgattaatgtggatagagtttctttt                                                                                              <  1:354284‑M1/150‑57 (MQ=255)                                                         ggAAGGCGTAATTCATCGATTTTGGGTGTTTTTAATTCATTAACATCACAAATGTTTTTTGATTGTGAAGTTTTGCACGGACGGGGAAGATGAgattcatccactacttgcatggatgagtaatgattaatgtggatagagtttcttttt                                                                                             <  2:859457‑M1/150‑58 (MQ=255)                                                           aaGGCGTAATTCATCGATTTTGGGTGTTTTTAATTCATTAACATCACAAATGTTTTTTGATTGTGAAGTTTTGCACGGACGGGGAAGATGAgattcatccactacttgcatggatgagtaatgattaatgtggatagagtttctttttga                                                                                           <  1:847210‑M1/150‑60 (MQ=255)                                                                gTAATTCATCGATTTTGGGTGTTTTTAATTCATTAACATCACAAATGTTTTTTGATTGTGAAGTTTTGCACGGACGGGGAAGATGAgattcatccactacttgcatggatgagtaatgattaatgtggatagagtttctttttgaggttg                                                                                      >  2:799819‑M1/1‑86 (MQ=255)                                                                       aTCGATTTTGGGTGTTTTTAATTCATTAACATCACAAATGTTTTTTGATTGTGAAGTTTTGCACGGACGGGGAAGATGAgattcatccactacttgcatggatgagtaatgattaatgtggatagagtttctttttgaggttggctaat                                                                                >  1:191524‑M1/1‑79 (MQ=255)                                                                          gATTTTGGGTGTTTTTAATTCATTAACATCACAAATGTTTTTTGATTGTGAAGTTTTGCACGGACGGGGAAGATGAgattcatccactacttgcatggatgagtaatgattaatgtggatagagtttctttttgaggttggctaatgaaa                                                                            >  2:464005‑M1/1‑76 (MQ=255)                                                                           aTTTTGGGTGTTTTTAATTCATTAACATCACAAATGTTTTTTGATTGTGAAGTTTTGCACGGACGGGGAAGATGAgattcatccactacttgcatggatgagtaatgattaatgtggatagagtttctttttgaggttggctaatgaaac                                                                           <  2:822660‑M1/150‑76 (MQ=255)                                                                             tttGGGTGTTTTTAATTCATTAACATCACAAATGTTTTTTGATTGTGAAGTTTTGCACGGACGGGGAAGATGAgattcatccactacttgcatggatgagtaatgattaatgtggatagagtttctttttgaggttggctaatgaaacgc                                                                         >  1:351346‑M1/1‑73 (MQ=255)                                                                              ttGGGTGTTTTTAATTCATTAACATCACAAATGTTTTTTGATTGTGAAGTTTTGCACGGACGGGGAAGATGAgattcatccactacttgcatggatgagtaatgattaatgtggatagagtttctttttgaggttggctaatgaaacgct                                                                        >  1:76204‑M1/1‑72 (MQ=255)                                                                                gggTGTTTTTAATTCATTAACATCACAAATGTTTTTTGATTGTGAAGTTTTGCACGGACGGGGAAGATGAgattcatccactacttgcatggatgagtaatgattaatgtggatagagtttctttttgaggttggctaatgaaacgctt                                                                       <  2:140824‑M1/149‑80 (MQ=255)                                                                                 ggTGTTTTTAATTCATTAACATCACAAATGTTTTTTGATTGTGAAGTTTTGCACGGACGGGGAAGATGAgattcatccactacttgcatggatgagtaatgattaatgtggatagagtttctttttgaggttggctaatgaaacgctttc                                                                     >  2:191648‑M1/1‑69 (MQ=255)                                                                                   tgtTTTTAATTCATTAACATCACAAATGTTTTTTGATTGTGAAGTTTTGCACGGACGGGGAAGATGAgattcatccactacttgcatggatgagtaatgattaatgtggatagagtttctttttgaggttggctaatgaaacgctttccg                                                                   <  1:1835‑M1/150‑84 (MQ=255)                                                                                         tAATTCATTAACATCACAAATGTTTTTTGATTGTGAAGTTTTGCACGGACGGGGAAGATGAgattcatccactacttgcatggatgagtaatgattaatgtggatagagtttctttttgaggttggctaatgaaacgctttccgctatt                                                              <  2:386561‑M1/149‑89 (MQ=255)                                                                                          aaTTCATTAACATCACAAATGTTTTTTGATTGTGAAGTTTTGCACGGACGGGGAAGATGAgattcatccactacttgcatggatgagtaatgattaatgtggatagagtttctttttgaggttggctaatgaaacgctttccgctattt                                                             <  2:52530‑M1/149‑90 (MQ=255)                                                                                            ttCATTAACATCACAAATGTTTTTTGATTGTGAAGTTTTGCACGGACGGGGAAGATGAgattcatccactacttgcatggatgagtaatgattaatgtggatagagtttctttttgaggttggctaatgaaacgctttccgctatttctt                                                          >  2:701074‑M1/1‑58 (MQ=255)                                                                                              cATTAACATCACAAATGTTTTTTGATTGTGAAGTTTTGCACGGACGGGGAAGATGAgattcatccactacttgcatggatgagtaatgattaatgtggatagagtttctttttgaggttggctaatgaaacgctttccgctatttcttt                                                         <  2:132692‑M1/149‑94 (MQ=255)                                                                                               aTTAACATCACAAATGTTTTTTGATTGTGAAGTTTTGCACGGACGGGGAAGATGAgattcatccactacttgcatggatgagtaatgattaatgtggatagagtttctttttgaggttggctaatgaaacgctttccgctatttctttta                                                       <  2:548507‑M1/150‑96 (MQ=255)                                                                                               aTTAACATCACAAATGTTTTTTGATTGTGAAGTTTTGCACGGACGGGGAAGATGAgattcatccactacttgcatggatgagtaatgattaatgtggatagagtttctttttgaggttggctaatgaaacgctttccgctatttctttt                                                        <  1:749181‑M1/149‑95 (MQ=255)                                                                                                ttAACATCACAAATGTTTTTTGATTGTGAAGTTTTGCACGGACGGGGAAGATGAgattcatccactacttgcatggatgagtaatgattaatgtggatagagtttctttttgaggttggctaatgaaacgctttccgctatttcttttat                                                      <  2:202926‑M1/150‑97 (MQ=255)                                                                                                  aaCATCACAAATGTTTTTTGATTGTGAAGTTTTGCACGGACGGGGAAGATGAgattcatccactacttgcatggatgagtaatgattaatgtggatagagtttctttttgaggttggctaatgaaacgctttccgctatttcttttatt                                                     >  2:851503‑M1/1‑52 (MQ=255)                                                                                                      tCACAAATGTTTTTTGATTGTGAAGTTTTGCACGGACGGGGAAGATGAgattcatccactacttgcatggatgagtaatgattaatgtggatagagtttctttttgaggttggctaatgaaacgctttccgctatttcttttattcaccc                                                <  1:418553‑M1/150‑103 (MQ=255)                                                                                                          aaaTGGTTTTTGATTGTGAAGTTTTGCACGGACGGGGAAGATGAgattcatccactacttgcatggatgagtaatgattaatgtggatagagtttctttttgaggttggctaatgaaacgctttccgctatttcttttattcaccctgct                                            >  2:363548‑M1/1‑44 (MQ=255)                                                                                                           aaTGTTTTTTGATTGTGAAGTTTTGCACGGACGGGGAAGATGAgattcatccactacttgcatggatgagtaatgattaatgtggatagagtttctttttgaggttggctaatgaaacgctttccgctatttcttttattcaccctgctc                                           <  1:191648‑M1/150‑108 (MQ=255)                                                                                                             tGTTTTTTGATTGTGAAGTTTTGCACGGACGGGGAAGATGAgattcatccactacttgcatggatgagtaatgattaatgtggatagagtttctttttgaggttggctaatgaaacgctttccgctatttcttttattcaccctgctcac                                         <  2:212306‑M1/150‑110 (MQ=255)                                                                                                               ttttttGATTGTGAAGTTTTGCACGGACGGGGAAGATGAgattcatccactacttgcatggatgagtaatgattaatgtggatagagtttctttttgaggttggctaatgaaacgctttccgctatttcttttattcaccctgctcacgc                                       >  2:140630‑M1/1‑39 (MQ=255)                                                                                                                     gATTGTGAAGTTTTGCACGGACGGGGAAGATGAgattcatccactacttgcatggatgagtaatgattaatgtggatagagtttctttttgaggttggctaatgaaacgctttccgctatttcttttattcaccctgctcacgctgtcca                                 >  2:565395‑M1/1‑33 (MQ=255)                                                                                                                      aTTGTGAAGTTTTGCACGGACGGGGAAGATGAgattcatccactacttgcatggatgagtaatgattaatgtggatagagtttctttttgaggttggctaatgaaacgctttccgctatttcttttattcaccctgctcacgctgtcca                                 <  2:56019‑M1/149‑118 (MQ=255)                                                                                                                                 ttGCACGGACGGGGAAGATGAgattcatccactacttgcatggatgagtaatgattaatgtggatagagtttctttttgaggttggctaatgaaacgctttccgctatttcttttattcaccctgctcacgctgtccaccgttccggcc                      <  2:688349‑M1/149‑129 (MQ=255)                                                                                                                                  tGCACGGACGGGGAAGATGAgattcatccactacttgcatggatgagtaatgattaatgtggatagagtttctttttgaggttggctaatgaaacgctttccgctatttcttttattcaccctgctcacgctgtccaccgttccggcc                      >  2:504097‑M1/1‑20 (MQ=255)                                                                                                                                   gCACGGACGGGGAAGATGAgattcatccactacttgcatggatgagtaatgattaatgtggatagagtttctttttgaggttggctaatgaaacgctttccgctatttcttttattcaccctgctcacgctgtccaccgttccggccca                    <  2:76204‑M1/149‑131 (MQ=255)                                                                                                                                         acggGGAAGATGAgattcatccactacttgcatggatgagtaatgattaatgtggatagagtttctttttgaggttggctaatgaaacgctttccgctatttcttttattcaccctgctcacgctgtccaccgttccggcccaggccgat             <  2:27568‑M1/150‑138 (MQ=255)                                                                                                                                           ggggAAGATGAgattcatccactacttgcatggatgagtaatgattaatgtggatagagtttctttttgaggttggctaatgaaacgctttccgctatttcttttattcaccctgctcacgctgtccaccgttccggcccaggccgatat           >  2:968038‑M1/1‑11 (MQ=255)                                                                                                                                               aaGATGAgattcatccactacttgcatggatgagtaatgattaatgtggatagagtttctttttgaggttggctaatgaaacgctttccgctatttcttttattcaccctgctcacgctgtccaccgttccggcccaggccgatattatc       <  1:504097‑M1/150‑144 (MQ=255)                                                                                                                                                aGATGAgattcatccactacttgcatggatgagtaatgattaatgtggatagagtttctttttgaggttggctaatgaaacgctttccgctatttcttttattcaccctgctcacgctgtccaccgttccggcccaggccgatattatcg      <  2:428941‑M1/150‑145 (MQ=255)                                                                                                                                                     agattcatccactacttgcatggatgagtaatgattaatgtggatagagtttctttttgaggttggctaatgaaacgctttccgctatttcttttattcaccctgctcacgctgtccaccgttccggcccaggccgatattatcgacga  <  2:278357‑M1/149‑149 (MQ=255)                                                                                                                                                     |                                                                                                                                                     TGGTGAGTGTCTTTAACGCTATTGCGCTGTCAGATGTAACTCTGTAAAACAGATCAGGAAGGCGTAATTCATCGATTTTGGGTGTTTTTAATTCATTAACATCACAAATGTTTTTTGATTGTGAAGTTTTGCACGGACGGGGAAGATGAATGAAAAAGATTGCATTTGGCTGTGATCATGTCGGTTTCATTTTAAAACATGAAATAGTGGCACATTTAGTTGAGCGTGGCGTTGAAGTGATTGATAAAGGAACCTGGTCGTCAGAGCGTACTGATTATCCACATTACGCCAGTCAAG  >  CP009273/4303018‑4303314

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑