Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
MC JC	CP009273	4,303,167	Δ450 bp	100%	coding (1‑450/450 nt)	rpiB →	ribose 5‑phosphate isomerase B/allose 6‑phosphate isomerase

Missing coverage evidence...
			seq id	start	end	size	←reads	reads→	gene	description
*	*	÷	CP009273	4303167	4303616	450	60 [0]	[0] 60	rpiB	ribose 5‑phosphate isomerase B/allose 6‑phosphate isomerase

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	CP009273	= 4303166	0 (0.000)	55 (0.980)	52/270	NT	100%	intergenic (‑358/‑1)	alsR/rpiB	d‑allose‑inducible als operon transcriptional repressor; autorepressor; repressor of rpiR/ribose 5‑phosphate isomerase B/allose 6‑phosphate isomerase
	?	CP009273	4303617 =	0 (0.000)					intergenic (+1/‑68)	rpiB/yjdP	ribose 5‑phosphate isomerase B/allose 6‑phosphate isomerase/putative periplasmic protein

ACACCAACGTGCTGGCTTTTGGTTCACGAGTGGTTGGCCTCGAACTGGCAAAAATGATTGTGGATGCGTGGCTGGGCGCACAGTACGAAGGCGGTCGTCATCAACAACGCGTGGAGGCGATTACGGCAATAGAGCAGCGGAGAAATTGAGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTCCACCGTTCCGGCCCAGGCCGATATTATCGACGA  >  CP009273/4303468‑4303764                                                                                                                                                      |                                                                                                                                                    tggtgagtgtctttaacgctattgcgctgtcagatgtaactctgtaaaacagatcaggaaggcgtaattcatcgattttgggtgtttttaattcattaacatcacaaatgttttttgattgtgaagttttgcacggacggggaagatgag                                                                                                                                                     <  2:125998‑M2/1‑1 (MQ=255)   gtgagtgtctttaacgctattgcgctgtcagatgtaactctgtaaaacagatcaggaaggcgtaattcatcgattttgggtgtttttaattcattaacatcacaaatgttttttgattgtgaagttttgcacggacggggaagatgaGAt                                                                                                                                                   <  1:967193‑M2/3‑1 (MQ=255)        tgtctttaacgctattgcgctgtcagatgtaactctgtaaaacagatcatgaaggcgtatttcatcgatttggggcgtttttaattcattaacatcacaaatgttttttgattgtgaagttttgcacggacgtggaagctgtGATTCATc                                                                                                                                              >  2:354284‑M2/143‑150 (MQ=255)        tgtctttaacgctattgcgctgtcagatgtaactctgtaaaacagatcaggaaggcgtaattcatcgattttgggtgtttttaattcattaacatcacaaatgttttttgattgtgaagttttgcacggacggggaagatgaGATTCATc                                                                                                                                              >  1:202926‑M2/143‑150 (MQ=255)         gtctttaacgctattgcgctgtcagatgtaactctgtaaaacagatcaggaaggcgtaattcatcgattttgggtgtttttaattcattaacatcacaaatgttttttgattgtgaagttttgcacggacggggaagatgaGATTCATc                                                                                                                                              >  1:386561‑M2/142‑149 (MQ=255)           ctttaacgctattgcgctgtcagatgtaactctgtaaaacagatcaggaaggcgtaattcatcgattttgggtgtttttaattcatttacatcacaaatgttttttgattgtgaagttttgcacggacggggaagatgaGATTCATCCac                                                                                                                                           >  2:511482‑M2/140‑150 (MQ=255)              taacgctattgcgctgtcagatgtaactctgtaaaacagatcaggaaggcgtaattcatcgattttgggtgtttttaattcattaacatcacaaatgttttttgattgtgaagttttgcacggacggggaagatgaGATTCATCCactac                                                                                                                                        >  1:508287‑M2/137‑150 (MQ=255)               aacgctattgcgctgtcagatgtaactctgtaaaacagatcaggaaggcgtaattcatcgattttgggtgtttttaattcattaacatcacaaatgttttttgattgtgaagttttgcacggacggggaagatgaGATTCATCCactact                                                                                                                                       >  1:428941‑M2/136‑150 (MQ=255)                    tattgcgctgtcagatgtaactctgtaaaacagatcaggaaggcgtaattcatcgattttgggtgtttttaattcattaacatcacaaatgttttttgattgtgaagttttgcacggacggggaagatgaGATTCATCCACTACTTGCAt                                                                                                                                  >  1:490587‑M2/131‑150 (MQ=255)                      ttgcgctgtcagatgtaactctgtaaaacagatcaggaaggcgtaattcatcgattttgggtgtttttaattcattaacatcacaaatgttttttgattgtgaagttttgcacggacggggaagatgaGATTCATCCACTACTTGCAt                                                                                                                                  <  1:78383‑M2/20‑1 (MQ=255)                         cgctgtcagatgtaactctgtaaaacagatcaggaaggcgtaattcatcgattttgggtgtttttaattcattaacatcacaaatgttttttgattgtgaagttttgcacggacggggaagatgaGATTCATCCACTACTTGCATGGAt                                                                                                                              <  2:664846‑M2/24‑1 (MQ=255)                          gctgtcagatgtaactctgtaaaacagatcaggaaggcgtaattcatcgattttgggtgtttttaattcattaacatcacaaatgttttttgattgtgaagttttgcacggacggggaagatgaGATTCATCCACTACTTGCATGGAt                                                                                                                              >  1:56019‑M2/125‑148 (MQ=255)                           ctgtcagatgtaactctgtaaaacagatcaggaaggcgtaattcatcgattttgggtgtttttaattcattaacatcacaaatgttttttgattgtgaagttttgcacggacggggaagatgaGATTCATCCACTACTTGCATGGATGAg                                                                                                                           <  1:712263‑M2/27‑1 (MQ=255)                            tgtcagatgtaactctgtaaaacagatcaggaaggcgtaattcatcgattttgggtgtttttaattcattaacatcacaaatgttttttgattgtgaagttttgcacggacggggaagatgaGATTCATCCACTACTTGCATGGATGAg                                                                                                                           >  2:418553‑M2/123‑149 (MQ=255)                            tgtcagatgtaactctgtaaaacagatcaggaaggcgtaattcatcgattttgggtgtttttaattcattaacatcacaaatgttttttgattgtgaagttttgcacggacggggaagatgaGATTCATCCACTACTTGCATGGATGAg                                                                                                                           >  1:27568‑M2/123‑149 (MQ=255)                             gtcagatgtaactctgtaaaacagatcaggaaggcgtaattcatcgattttgggtgtttttaattcattaacatcacaaatgttttttgattgtgaagttttgcacggacggggaagatgaGATTCATCCACTACTTGCATGGATGAGt                                                                                                                          >  2:414945‑M2/122‑149 (MQ=255)                             gtcagatgtaactctgtaaaacagatcaggaaggcgtaattcatcgattttgggtgtttttaattcattaacatcacaaatgttttttgattgtgaagttttgcacggacggggaagatgaGATTCATCCACTACTTGCATGGATGAGt                                                                                                                          >  1:859457‑M2/122‑149 (MQ=255)                              tcagatgtaactctgtaaaacagatcaggaaggcgtaattcatcgattttgggtgtttttaattcattaacatcacaaatgttttttgattgtgaagttttgcacggacggggaagatgaGATTCATCCACTACTTGCATGGATGAGTa                                                                                                                         <  2:214438‑M2/29‑1 (MQ=255)                                  atgtaactctgtaaaacagatcaggaaggcgtaattcatcgattttgggtgtttttaattcattaacatcacaaatgttttttgattgtgaagttttgcacggacggggaagatgaGATTCATCCACTACTTGCATGGATGAGTAATGAt                                                                                                                    <  2:944112‑M2/34‑1 (MQ=255)                                     taactctgtaaaacagatcaggaaggcgtaattcatcgattttgggtgtttttaattcattaacatcacaaatgttttttgattgtgaagttttgcacggacggggaagatgaGATTCATCCACTACTTGCATGGATGAGTAATGATTaa                                                                                                                 >  1:212306‑M2/114‑150 (MQ=255)                                           tgtaaaacagatcaggaaggcgtaattcatcgattttgggtgtttttaattcattaacatcacaaatgttttttgattgtgaagttttgcacggacggggaagatgaGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGa                                                                                                           >  1:548507‑M2/108‑150 (MQ=255)                                            gtaaaacagatcaggaaggcgtaattcatcgattttgggtgtttttaattcattaacatcacaaatgttttttgattgtgaagttttgcacggacggggaagatgaGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGa                                                                                                           >  1:688349‑M2/107‑149 (MQ=255)                                             taaaacagatcaggaaggcgtaattcatcgattttgggtgtttttaattcattaacatcacaaatgttttttgattgtgaagttttgcacggacggggaagatgaGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTgg                                                                                                            <  2:217756‑M2/42‑1 (MQ=255)                                             taaaacagatcaggaaggcgtaattcatcgattttgggtgtttttaattcattaacatcacaaatgttttttgattgtgaagttttgcacggacggggaagatgaGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATa                                                                                                         <  1:67512‑M2/45‑1 (MQ=255)                                                        aggaaggcgtaattcatcgattttgggtgtttttaattcattaacatcacaaatgttttttgattgtgaagttttgcacggacggggaagatgaGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGTTTCtttt                                                                                              >  1:987366‑M2/95‑150 (MQ=255)                                                        aggaaggcgtaattaatcgattttgggtgtttttaattcattaacatcacaaatgttttttgattgtgaagttttgcacggacggggaagatgaGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGTTTCtttt                                                                                              <  1:354284‑M2/56‑1 (MQ=255)                                                         ggaaggcgtaattcatcgattttgggtgtttttaattcattaacatcacaaatgttttttgattgtgaagttttgcacggacggggaagatgaGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGTTTCttttt                                                                                             <  2:859457‑M2/57‑1 (MQ=255)                                                           aaggcgtaattcatcgattttgggtgtttttaattcattaacatcacaaatgttttttgattgtgaagttttgcacggacggggaagatgaGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGTTTCTTTTTGa                                                                                           <  1:847210‑M2/59‑1 (MQ=255)                                                                gtaattcatcgattttgggtgtttttaattcattaacatcacaaatgttttttgattgtgaagttttgcacggacggggaagatgaGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTg                                                                                      >  2:799819‑M2/87‑150 (MQ=255)                                                                       atcgattttgggtgtttttaattcattaacatcacaaatgttttttgattgtgaagttttgcacggacggggaagatgaGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAAt                                                                                >  1:191524‑M2/80‑149 (MQ=255)                                                                          gattttgggtgtttttaattcattaacatcacaaatgttttttgattgtgaagttttgcacggacggggaagatgaGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGaaa                                                                            >  2:464005‑M2/77‑150 (MQ=255)                                                                           attttgggtgtttttaattcattaacatcacaaatgttttttgattgtgaagttttgcacggacggggaagatgaGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAAc                                                                           <  2:822660‑M2/75‑1 (MQ=255)                                                                             tttgggtgtttttaattcattaacatcacaaatgttttttgattgtgaagttttgcacggacggggaagatgaGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGc                                                                         >  1:351346‑M2/74‑150 (MQ=255)                                                                              ttgggtgtttttaattcattaacatcacaaatgttttttgattgtgaagttttgcacggacggggaagatgaGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCt                                                                        >  1:76204‑M2/73‑150 (MQ=255)                                                                                gggtgtttttaattcattaacatcacaaatgttttttgattgtgaagttttgcacggacggggaagatgaGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCtt                                                                       <  2:140824‑M2/79‑1 (MQ=255)                                                                                 ggtgtttttaattcattaacatcacaaatgttttttgattgtgaagttttgcacggacggggaagatgaGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTc                                                                     >  2:191648‑M2/70‑150 (MQ=255)                                                                                   tgtttttaattcattaacatcacaaatgttttttgattgtgaagttttgcacggacggggaagatgaGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCg                                                                   <  1:1835‑M2/83‑1 (MQ=255)                                                                                         taattcattaacatcacaaatgttttttgattgtgaagttttgcacggacggggaagatgaGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTAtt                                                              <  2:386561‑M2/88‑1 (MQ=255)                                                                                          aattcattaacatcacaaatgttttttgattgtgaagttttgcacggacggggaagatgaGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTAttt                                                             <  2:52530‑M2/89‑1 (MQ=255)                                                                                            ttcattaacatcacaaatgttttttgattgtgaagttttgcacggacggggaagatgaGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCtt                                                          >  2:701074‑M2/59‑150 (MQ=255)                                                                                              cattaacatcacaaatgttttttgattgtgaagttttgcacggacggggaagatgaGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCttt                                                         <  2:132692‑M2/93‑1 (MQ=255)                                                                                               attaacatcacaaatgttttttgattgtgaagttttgcacggacggggaagatgaGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCtttt                                                        <  1:749181‑M2/94‑1 (MQ=255)                                                                                               attaacatcacaaatgttttttgattgtgaagttttgcacggacggggaagatgaGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTa                                                       <  2:548507‑M2/95‑1 (MQ=255)                                                                                                ttaacatcacaaatgttttttgattgtgaagttttgcacggacggggaagatgaGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTAt                                                      <  2:202926‑M2/96‑1 (MQ=255)                                                                                                  aacatcacaaatgttttttgattgtgaagttttgcacggacggggaagatgaGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTAtt                                                     >  2:851503‑M2/53‑149 (MQ=255)                                                                                                      tcacaaatgttttttgattgtgaagttttgcacggacggggaagatgaGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCAccc                                                <  1:418553‑M2/102‑1 (MQ=255)                                                                                                          aaatggtttttgattgtgaagttttgcacggacggggaagatgaGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCt                                            >  2:363548‑M2/45‑150 (MQ=255)                                                                                                           aatgttttttgattgtgaagttttgcacggacggggaagatgaGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTc                                           <  1:191648‑M2/107‑1 (MQ=255)                                                                                                             tgttttttgattgtgaagttttgcacggacggggaagatgaGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCAc                                         <  2:212306‑M2/109‑1 (MQ=255)                                                                                                               ttttttgattgtgaagttttgcacggacggggaagatgaGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGc                                       >  2:140630‑M2/40‑150 (MQ=255)                                                                                                                     gattgtgaagttttgcacggacggggaagatgaGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTCCa                                 >  2:565395‑M2/34‑150 (MQ=255)                                                                                                                      attgtgaagttttgcacggacggggaagatgaGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTCCa                                 <  2:56019‑M2/117‑1 (MQ=255)                                                                                                                                 ttgcacggacggggaagatgaGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTCCACCGTTCCGGcc                      <  2:688349‑M2/128‑1 (MQ=255)                                                                                                                                  tgcacggacggggaagatgaGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTCCACCGTTCCGGcc                      >  2:504097‑M2/21‑148 (MQ=255)                                                                                                                                   gcacggacggggaagatgaGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTCCACCGTTCCGGCCCa                    <  2:76204‑M2/130‑1 (MQ=255)                                                                                                                                         acggggaagatgaGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTCCACCGTTCCGGCCCAGGCCGat             <  2:27568‑M2/137‑1 (MQ=255)                                                                                                                                           ggggaagatgaGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTCCACCGTTCCGGCCCAGGCCGatat           >  2:968038‑M2/12‑150 (MQ=255)                                                                                                                                               aagatgaGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTCCACCGTTCCGGCCCAGGCCGATATTATc       <  1:504097‑M2/143‑1 (MQ=255)                                                                                                                                                agatgaGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTCCACCGTTCCGGCCCAGGCCGATATTATcg      <  2:428941‑M2/144‑1 (MQ=255)                                                                                                                                                     aGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTCCACCGTTCCGGCCCAGGCCGATATTATcgacga  <  2:278357‑M2/148‑1 (MQ=255)                                                                                                                                                      |                                                                                                                                                    ACACCAACGTGCTGGCTTTTGGTTCACGAGTGGTTGGCCTCGAACTGGCAAAAATGATTGTGGATGCGTGGCTGGGCGCACAGTACGAAGGCGGTCGTCATCAACAACGCGTGGAGGCGATTACGGCAATAGAGCAGCGGAGAAATTGAGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTCCACCGTTCCGGCCCAGGCCGATATTATCGACGA  >  CP009273/4303468‑4303764

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑