breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Marginal read alignment evidence (highest frequency 20 of 29 shown, sorted by frequency from high to low)
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AP0123061,091,9920TG44.2% 342.5 / 81.9 240intergenic (+38/‑145)narI/ychSnitrate reductase 1, gamma (cytochrome b(NR)) subunit/predicted protein
*AP0123061,944,2880AC40.5% 338.1 / 65.2 210G361G (GGT→GGGmenEo‑succinylbenzoate‑CoA ligase
*AP0123062,758,9090TG35.3% 423.3 / 39.8 207G33G (GGT→GGGagaDN‑acetylgalactosamine‑specific enzyme IID component of PTS
*AP0123063,527,3680AC32.3% 548.2 / 65.2 251L140F (TTA→TTCrhaSDNA‑binding transcriptional activator, L‑rhamnose‑binding
*AP0123063,639,0400TG28.8% 95.9 / 16.5 52noncoding (54/76 nt)gltVtRNA‑Glu
*AP012306341,3770TG28.4% 209.2 / 20.7 116G60G (GGT→GGGribDfused diaminohydroxyphosphoribosylaminopyrimidin e deaminase and 5‑amino‑6‑(5‑phosphoribosylamino) uracil reductase
*AP0123063,282,0760TG28.0% 427.3 / 22.2 218intergenic (‑79/+27)ilvB/ivbLacetolactate synthase I, large subunit/ilvB operon leader peptide
*AP012306644,4170GA28.0% 1011.7 / inf 572intergenic (+32/‑401)lysQ/nadAtRNA‑Lys/quinolinate synthase, subunit A
*AP0123063,938,8870CT27.7% 541.1 / 155.9 264S44F (TCT→TTT) bglJDNA‑binding transcriptional regulator
*AP0123062,680,3450AC26.8% 435.9 / 30.8 198T184P (ACC→CCC) ttdAL‑tartrate dehydratase, alpha subunit
*AP012306800,6750AC26.0% 640.7 / 34.1 266T192P (ACC→CCC) lolAchaperone for lipoproteins
*AP012306202,4170TG25.4% 521.9 / 30.5 244V409G (GTA→GGA) tilStRNA(Ile)‑lysidine synthetase
*AP012306935,0540GT25.0% 241.7 / 36.8 88intergenic (+24/‑1406)putP/ycdOproline:sodium symporter/conserved protein
*AP0123061,226,5570AG24.3% 338.9 / 61.2 152G55G (GGA→GGGydcUpredicted spermidine/putrescine transporter subunit
*AP0123063,716,9320TA24.1% 564.1 / 128.7 228intergenic (‑348/‑45)acs/nrfAbifunctional acetyl‑CoA synthetase/nitrite reductase, formate‑dependent, cytochrome
*AP0123063,903,7910AG24.0% 540.3 / 67.2 229E189G (GAA→GGA) yjgJpredicted transcriptional regulator
*AP0123061,128,4120TG23.6% 633.1 / 38.2 255V29G (GTG→GGG) yciOconserved protein
*AP0123062,558,0710AC23.1% 544.5 / 24.9 225V302G (GTA→GGA) visCpredicted oxidoreductase, FAD/NAD(P)‑binding domain
*AP012306524,2970CT22.8% 348.6 / 40.3 145M1M (ATG→ATA) †citDcitrate lyase, acyl carrier (gamma) subunit
*AP0123063,590,3670AC22.1% 742.1 / 20.3 258T30P (ACC→CCC) fabRDNA‑binding transcriptional repressor

Marginal new junction evidence (lowest skew 10 of 62 shown)
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? AP012306 = 1092512398 (1.270)21 (0.080) 13/258 6.2 6.3% intergenic (+100/+3) ychS/tpr predicted protein/predicted protamine‑like protein
?AP012306 1092985 = 280 (1.030)noncoding (66/85 nt) tyrT tRNA‑Tyr
* ? AP012306 = 215364NA (NA)9 (0.030) 9/288 7.3 NA noncoding (745/2905 nt) rrlH 23S ribosomal RNA (rrlH)
?AP012306 = 215372 NA (NA)noncoding (753/2905 nt) rrlH 23S ribosomal RNA (rrlH)
* ? AP012306 = 1092334336 (1.070)10 (0.040) 7/258 7.5 3.4% coding (198/276 nt) ychS predicted protein
?AP012306 1092985 = 280 (1.030)noncoding (66/85 nt) tyrT tRNA‑Tyr
* ? AP012306 = 3224037345 (1.100)7 (0.020) 7/286 7.8 2.1% coding (837/933 nt) rfaD ADP‑L‑glycero‑D‑mannoheptose‑6‑epimerase, NAD(P)‑binding
?AP012306 = 3224044 333 (1.110)coding (844/933 nt) rfaD ADP‑L‑glycero‑D‑mannoheptose‑6‑epimerase, NAD(P)‑binding
* ? AP012306 = 2659620253 (0.810)6 (0.020) 6/274 7.9 2.6% coding (1257/1482 nt) tolC transport channel
?AP012306 = 2659608 219 (0.760)coding (1245/1482 nt) tolC transport channel
* ? AP012306 186025 =297 (0.950)5 (0.020) 5/286 8.3 1.7% coding (619/1353 nt) yaeL zinc metallopeptidase
?AP012306 186042 = 282 (0.940)coding (636/1353 nt) yaeL zinc metallopeptidase
* ? AP012306 2883249 =276 (0.880)5 (0.020) 5/290 8.4 1.9% coding (365/3105 nt) acrF multidrug efflux system protein
?AP012306 2883275 = 239 (0.780)coding (391/3105 nt) acrF multidrug efflux system protein
* ? AP012306 2766713 =360 (1.150)5 (0.020) 5/288 8.4 1.4% intergenic (‑45/‑20) yraL/yraM predicted methyltransferase/conserved protein
?AP012306 2766752 = 362 (1.200)coding (20/2037 nt) yraM conserved protein
* ? AP012306 = 3853073171 (0.550)4 (0.010) 4/282 8.6 2.4% coding (272/687 nt) ulaF L‑ribulose 5‑phosphate 4‑epimerase
?AP012306 = 3853093 166 (0.560)coding (292/687 nt) ulaF L‑ribulose 5‑phosphate 4‑epimerase
* ? AP012306 = 2548832281 (0.900)4 (0.010) 4/282 8.6 1.5% coding (862/981 nt) ygfZ predicted folate‑dependent regulatory protein
?AP012306 = 2548881 248 (0.840)coding (911/981 nt) ygfZ predicted folate‑dependent regulatory protein