breseq version 0.35.4 revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log |
Marginal read alignment evidence (highest frequency 20 of 33 shown, sorted by frequency from high to low) | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | AP012306 | 1,091,992 | 0 | T | G | 40.2% | 509.6 / 75.8 | 302 | intergenic (+38/‑145) | narI/ychS | nitrate reductase 1, gamma (cytochrome b(NR)) subunit/predicted protein |
* | AP012306 | 3,938,887 | 0 | C | T | 32.3% | 428.8 / 185.9 | 251 | S44F (TCT→TTT) | bglJ | DNA‑binding transcriptional regulator |
* | AP012306 | 644,417 | 0 | G | A | 31.6% | 775.5 / inf | 541 | intergenic (+32/‑401) | lysQ/nadA | tRNA‑Lys/quinolinate synthase, subunit A |
* | AP012306 | 1,944,288 | 0 | A | C | 29.3% | 555.1 / 53.1 | 246 | G361G (GGT→GGG) | menE | o‑succinylbenzoate‑CoA ligase |
* | AP012306 | 2,758,909 | 0 | T | G | 29.2% | 574.0 / 23.3 | 241 | G33G (GGT→GGG) | agaD | N‑acetylgalactosamine‑specific enzyme IID component of PTS |
* | AP012306 | 341,377 | 0 | T | G | 28.5% | 304.6 / 27.2 | 158 | G60G (GGT→GGG) | ribD | fused diaminohydroxyphosphoribosylaminopyrimidin e deaminase and 5‑amino‑6‑(5‑phosphoribosylamino) uracil reductase |
* | AP012306 | 3,182,861 | 0 | A | C | 26.6% | 318.8 / 18.4 | 128 | L198F (TTA→TTC) | yiaN | predicted transporter |
* | AP012306 | 1,226,557 | 0 | A | G | 26.3% | 496.0 / 104.9 | 228 | G55G (GGA→GGG) | ydcU | predicted spermidine/putrescine transporter subunit |
* | AP012306 | 1,854,851 | 0 | A | C | 26.1% | 689.7 / 58.1 | 276 | T25P (ACC→CCC) | yejH | predicted ATP‑dependet helicase |
* | AP012306 | 3,527,368 | 0 | A | C | 26.0% | 737.0 / 56.1 | 296 | L140F (TTA→TTC) | rhaS | DNA‑binding transcriptional activator, L‑rhamnose‑binding |
* | AP012306 | 800,675 | 0 | A | C | 25.6% | 709.0 / 40.3 | 293 | T192P (ACC→CCC) | lolA | chaperone for lipoproteins |
* | AP012306 | 1,421,147 | 0 | T | G | 25.4% | 513.9 / 13.0 | 244 | T79P (ACC→CCC) | ydhB | predicted DNA‑binding transcriptional regulator |
* | AP012306 | 1,309,914 | 0 | A | C | 25.3% | 408.8 / 11.9 | 194 | L23F (TTA→TTC) | lsrC | AI2 transporter |
* | AP012306 | 2,714,555 | 0 | T | G | 25.1% | 749.7 / 40.1 | 315 | T175P (ACC→CCC) | ygjV | conserved inner membrane protein |
* | AP012306 | 406,677 | 0 | A | G | 24.1% | 563.1 / 30.7 | 224 | K294E (AAA→GAA) | hemH | ferrochelatase |
* | AP012306 | 1,393,379 | 0 | A | C | 23.9% | 823.8 / 61.8 | 319 | T92P (ACC→CCC) | nth | DNA glycosylase and apyrimidinic (AP) lyase |
* | AP012306 | 1,128,412 | 0 | T | G | 23.8% | 892.8 / 55.6 | 344 | V29G (GTG→GGG) | yciO | conserved protein |
* | AP012306 | 2,857,911 | 0 | A | C | 23.3% | 589.7 / 23.9 | 219 | K180N (AAA→AAC) | aaeR | predicted DNA‑binding transcriptional regulator, efflux system |
* | AP012306 | 1,066,323 | 0 | A | C | 22.9% | 807.5 / 13.3 | 293 | intergenic (‑92/+33) | ychM/prsA | predicted transporter/phosphoribosylpyrophosphate synthase |
* | AP012306 | 3,182,506 | 0 | A | C | 22.5% | 187.6 / 11.1 | 81 | D80A (GAC→GCC) | yiaN | predicted transporter |
Marginal new junction evidence (lowest skew 10 of 92 shown) | |||||||||||
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seq id | position | reads (cov) | reads (cov) | score | skew | freq | annotation | gene | product | ||
* | ? | AP012306 | 644310 = | 387 (1.140) | 32 (0.100) +GAG |
15/292 | 19.8 | 8.0% | noncoding (1/76 nt) | lysQ | tRNA‑Lys |
? | AP012306 | = 2073420 | 367 (1.080) | noncoding (76/76 nt) | valU | tRNA‑Val | |||||
* | ? | AP012306 | 644310 = | 387 (1.140) | 30 (0.090) +G |
13/296 | 20.7 | 8.0% | noncoding (1/76 nt) | lysQ | tRNA‑Lys |
? | AP012306 | = 2073542 | 304 (0.890) | intergenic (+2/‑45) | valX/valY | tRNA‑Val/tRNA‑Val | |||||
* | ? | AP012306 | 352909 = | 328 (0.960) | 7 (0.020) | 7/270 | 22.9 | 2.3% | coding (484/492 nt) | yajQ | predicted nucleotide binding protein |
? | AP012306 | 352923 = | 290 (0.940) | intergenic (+6/+122) | yajQ/yajR | predicted nucleotide binding protein/predicted transporter | |||||
* | ? | AP012306 | 1042309 = | 406 (1.190) | 8 (0.020) | 8/290 | 23.0 | 2.0% | coding (361/1533 nt) | ycgB | conserved protein |
? | AP012306 | 1042336 = | 374 (1.130) | coding (334/1533 nt) | ycgB | conserved protein | |||||
* | ? | AP012306 | = 215364 | NA (NA) | 8 (0.020) | 8/288 | 23.0 | NA | noncoding (745/2905 nt) | rrlH | 23S ribosomal RNA (rrlH) |
? | AP012306 | = 215372 | NA (NA) | noncoding (753/2905 nt) | rrlH | 23S ribosomal RNA (rrlH) | |||||
* | ? | AP012306 | = 1092334 | 349 (1.030) | 8 (0.030) | 6/258 | 23.1 | 2.4% | coding (198/276 nt) | ychS | predicted protein |
? | AP012306 | 1092985 = | 340 (1.160) | noncoding (66/85 nt) | tyrT | tRNA‑Tyr | |||||
* | ? | AP012306 | = 1940802 | 369 (1.080) | 7 (0.020) | 7/292 | 23.7 | 1.9% | coding (418/891 nt) | yfbH | conserved protein |
? | AP012306 | = 1940814 | 353 (1.060) | coding (430/891 nt) | yfbH | conserved protein | |||||
* | ? | AP012306 | = 59231 | 310 (0.910) | 7 (0.020) | 6/274 | 23.7 | 2.4% | coding (1375/2352 nt) | polB | DNA polymerase II |
? | AP012306 | = 59219 | 280 (0.900) | coding (1387/2352 nt) | polB | DNA polymerase II | |||||
* | ? | AP012306 | = 1092512 | 431 (1.270) | 5 (0.020) | 5/258 | 23.8 | 1.4% | intergenic (+100/+3) | ychS/tpr | predicted protein/predicted protamine‑like protein |
? | AP012306 | 1092985 = | 340 (1.160) | noncoding (66/85 nt) | tyrT | tRNA‑Tyr | |||||
* | ? | AP012306 | = 2098344 | 290 (0.850) | 5 (0.020) | 5/270 | 24.2 | 1.9% | coding (155/897 nt) | yfeU | predicted PTS component |
? | AP012306 | = 2098330 | 263 (0.850) | coding (141/897 nt) | yfeU | predicted PTS component |