breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Marginal read alignment evidence (highest frequency 20 of 33 shown, sorted by frequency from high to low)
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AP0123061,091,9920TG40.2% 509.6 / 75.8 302intergenic (+38/‑145)narI/ychSnitrate reductase 1, gamma (cytochrome b(NR)) subunit/predicted protein
*AP0123063,938,8870CT32.3% 428.8 / 185.9 251S44F (TCT→TTT) bglJDNA‑binding transcriptional regulator
*AP012306644,4170GA31.6% 775.5 / inf 541intergenic (+32/‑401)lysQ/nadAtRNA‑Lys/quinolinate synthase, subunit A
*AP0123061,944,2880AC29.3% 555.1 / 53.1 246G361G (GGT→GGGmenEo‑succinylbenzoate‑CoA ligase
*AP0123062,758,9090TG29.2% 574.0 / 23.3 241G33G (GGT→GGGagaDN‑acetylgalactosamine‑specific enzyme IID component of PTS
*AP012306341,3770TG28.5% 304.6 / 27.2 158G60G (GGT→GGGribDfused diaminohydroxyphosphoribosylaminopyrimidin e deaminase and 5‑amino‑6‑(5‑phosphoribosylamino) uracil reductase
*AP0123063,182,8610AC26.6% 318.8 / 18.4 128L198F (TTA→TTCyiaNpredicted transporter
*AP0123061,226,5570AG26.3% 496.0 / 104.9 228G55G (GGA→GGGydcUpredicted spermidine/putrescine transporter subunit
*AP0123061,854,8510AC26.1% 689.7 / 58.1 276T25P (ACC→CCC) yejHpredicted ATP‑dependet helicase
*AP0123063,527,3680AC26.0% 737.0 / 56.1 296L140F (TTA→TTCrhaSDNA‑binding transcriptional activator, L‑rhamnose‑binding
*AP012306800,6750AC25.6% 709.0 / 40.3 293T192P (ACC→CCC) lolAchaperone for lipoproteins
*AP0123061,421,1470TG25.4% 513.9 / 13.0 244T79P (ACC→CCC) ydhBpredicted DNA‑binding transcriptional regulator
*AP0123061,309,9140AC25.3% 408.8 / 11.9 194L23F (TTA→TTClsrCAI2 transporter
*AP0123062,714,5550TG25.1% 749.7 / 40.1 315T175P (ACC→CCC) ygjVconserved inner membrane protein
*AP012306406,6770AG24.1% 563.1 / 30.7 224K294E (AAA→GAA) hemHferrochelatase
*AP0123061,393,3790AC23.9% 823.8 / 61.8 319T92P (ACC→CCC) nthDNA glycosylase and apyrimidinic (AP) lyase
*AP0123061,128,4120TG23.8% 892.8 / 55.6 344V29G (GTG→GGG) yciOconserved protein
*AP0123062,857,9110AC23.3% 589.7 / 23.9 219K180N (AAA→AACaaeRpredicted DNA‑binding transcriptional regulator, efflux system
*AP0123061,066,3230AC22.9% 807.5 / 13.3 293intergenic (‑92/+33)ychM/prsApredicted transporter/phosphoribosylpyrophosphate synthase
*AP0123063,182,5060AC22.5% 187.6 / 11.1 81D80A (GAC→GCC) yiaNpredicted transporter

Marginal new junction evidence (lowest skew 10 of 92 shown)
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? AP012306 644310 =387 (1.140)32 (0.100)
+GAG
15/292 19.8 8.0% noncoding (1/76 nt) lysQ tRNA‑Lys
?AP012306 = 2073420 367 (1.080)noncoding (76/76 nt) valU tRNA‑Val
* ? AP012306 644310 =387 (1.140)30 (0.090)
+G
13/296 20.7 8.0% noncoding (1/76 nt) lysQ tRNA‑Lys
?AP012306 = 2073542 304 (0.890)intergenic (+2/‑45) valX/valY tRNA‑Val/tRNA‑Val
* ? AP012306 352909 =328 (0.960)7 (0.020) 7/270 22.9 2.3% coding (484/492 nt) yajQ predicted nucleotide binding protein
?AP012306 352923 = 290 (0.940)intergenic (+6/+122) yajQ/yajR predicted nucleotide binding protein/predicted transporter
* ? AP012306 1042309 =406 (1.190)8 (0.020) 8/290 23.0 2.0% coding (361/1533 nt) ycgB conserved protein
?AP012306 1042336 = 374 (1.130)coding (334/1533 nt) ycgB conserved protein
* ? AP012306 = 215364NA (NA)8 (0.020) 8/288 23.0 NA noncoding (745/2905 nt) rrlH 23S ribosomal RNA (rrlH)
?AP012306 = 215372 NA (NA)noncoding (753/2905 nt) rrlH 23S ribosomal RNA (rrlH)
* ? AP012306 = 1092334349 (1.030)8 (0.030) 6/258 23.1 2.4% coding (198/276 nt) ychS predicted protein
?AP012306 1092985 = 340 (1.160)noncoding (66/85 nt) tyrT tRNA‑Tyr
* ? AP012306 = 1940802369 (1.080)7 (0.020) 7/292 23.7 1.9% coding (418/891 nt) yfbH conserved protein
?AP012306 = 1940814 353 (1.060)coding (430/891 nt) yfbH conserved protein
* ? AP012306 = 59231310 (0.910)7 (0.020) 6/274 23.7 2.4% coding (1375/2352 nt) polB DNA polymerase II
?AP012306 = 59219 280 (0.900)coding (1387/2352 nt) polB DNA polymerase II
* ? AP012306 = 1092512431 (1.270)5 (0.020) 5/258 23.8 1.4% intergenic (+100/+3) ychS/tpr predicted protein/predicted protamine‑like protein
?AP012306 1092985 = 340 (1.160)noncoding (66/85 nt) tyrT tRNA‑Tyr
* ? AP012306 = 2098344290 (0.850)5 (0.020) 5/270 24.2 1.9% coding (155/897 nt) yfeU predicted PTS component
?AP012306 = 2098330 263 (0.850)coding (141/897 nt) yfeU predicted PTS component