breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Marginal read alignment evidence (highest frequency 20 of 29 shown, sorted by frequency from high to low)
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AP0123061,091,9920TG39.3% 427.5 / 69.0 253intergenic (+38/‑145)narI/ychSnitrate reductase 1, gamma (cytochrome b(NR)) subunit/predicted protein
*AP0123061,944,2880AC37.6% 331.3 / 53.9 202G361G (GGT→GGGmenEo‑succinylbenzoate‑CoA ligase
*AP012306644,4170GA31.8% 908.9 / inf 607intergenic (+32/‑401)lysQ/nadAtRNA‑Lys/quinolinate synthase, subunit A
*AP0123061,309,9140AC31.6% 263.7 / 10.2 136L23F (TTA→TTClsrCAI2 transporter
*AP0123063,938,8870CT31.5% 480.0 / 185.2 276S44F (TCT→TTT) bglJDNA‑binding transcriptional regulator
*AP012306800,6750AC30.6% 547.2 / 44.7 262T192P (ACC→CCC) lolAchaperone for lipoproteins
*AP012306935,0540GT29.7% 148.2 / 35.3 64intergenic (+24/‑1406)putP/ycdOproline:sodium symporter/conserved protein
*AP0123062,758,9090TG29.3% 608.4 / 35.1 267G33G (GGT→GGGagaDN‑acetylgalactosamine‑specific enzyme IID component of PTS
*AP012306202,4170TG28.5% 598.8 / 39.0 271V409G (GTA→GGA) tilStRNA(Ile)‑lysidine synthetase
*AP012306341,3770TG27.4% 203.3 / 17.2 106G60G (GGT→GGGribDfused diaminohydroxyphosphoribosylaminopyrimidin e deaminase and 5‑amino‑6‑(5‑phosphoribosylamino) uracil reductase
*AP0123063,527,3680AC26.9% 738.8 / 54.7 305L140F (TTA→TTCrhaSDNA‑binding transcriptional activator, L‑rhamnose‑binding
*AP0123063,903,7910AG24.6% 679.0 / 76.8 285E189G (GAA→GGA) yjgJpredicted transcriptional regulator
*AP0123061,128,4120TG24.2% 670.3 / 68.2 289V29G (GTG→GGG) yciOconserved protein
*AP012306406,6770AG24.0% 552.1 / 24.7 221K294E (AAA→GAA) hemHferrochelatase
*AP0123062,857,9110AC23.7% 518.8 / 12.1 198K180N (AAA→AACaaeRpredicted DNA‑binding transcriptional regulator, efflux system
*AP0123061,854,8510AC23.3% 773.9 / 62.1 309T25P (ACC→CCC) yejHpredicted ATP‑dependet helicase
*AP0123061,066,3230AC23.2% 763.7 / 18.8 280intergenic (‑92/+33)ychM/prsApredicted transporter/phosphoribosylpyrophosphate synthase
*AP0123062,212,7530AC23.1% 339.7 / 34.4 134Y241D (TAT→GAT) hcaTpredicted 3‑phenylpropionic transporter
*AP0123062,558,0710AC22.7% 590.4 / 32.4 229V302G (GTA→GGA) visCpredicted oxidoreductase, FAD/NAD(P)‑binding domain
*AP0123063,282,0760TG22.7% 591.7 / 12.0 252intergenic (‑79/+27)ilvB/ivbLacetolactate synthase I, large subunit/ilvB operon leader peptide

Marginal new junction evidence (lowest skew 10 of 283 shown)
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? AP012306 = 2275012305 (0.910)14 (0.050) 14/274 10.4 4.8% noncoding (60/76 nt) gltW tRNA‑Glu
?AP012306 3372719 = NA (NA)noncoding (72/76 nt) gltU tRNA‑Glu
* ? AP012306 644310 =439 (1.310)35 (0.110)
+GAG
14/292 10.7 8.2% noncoding (1/76 nt) lysQ tRNA‑Lys
?AP012306 = 2073420 364 (1.080)noncoding (76/76 nt) valU tRNA‑Val
* ? AP012306 = 1092512346 (1.030)13 (0.040) 11/258 10.9 4.4% intergenic (+100/+3) ychS/tpr predicted protein/predicted protamine‑like protein
?AP012306 1092985 = 268 (0.920)noncoding (66/85 nt) tyrT tRNA‑Tyr
* ? AP012306 3597644 =242 (0.720)11 (0.040) 11/274 11.2 4.7% noncoding (60/76 nt) gltT tRNA‑Glu
?AP012306 3639058 = NA (NA)noncoding (72/76 nt) gltV tRNA‑Glu
* ? AP012306 = 2275012305 (0.910)11 (0.040) 11/274 11.2 3.6% noncoding (60/76 nt) gltW tRNA‑Glu
?AP012306 = 2275000 303 (0.980)noncoding (72/76 nt) gltW tRNA‑Glu
* ? AP012306 644310 =439 (1.310)32 (0.100)
+G
12/296 11.3 7.5% noncoding (1/76 nt) lysQ tRNA‑Lys
?AP012306 = 2073542 351 (1.040)intergenic (+2/‑45) valX/valY tRNA‑Val/tRNA‑Val
* ? AP012306 = 2275012305 (0.910)9 (0.030) 9/274 11.8 3.3% noncoding (60/76 nt) gltW tRNA‑Glu
?AP012306 3597656 = 242 (0.780)noncoding (72/76 nt) gltT tRNA‑Glu
* ? AP012306 2670435 =288 (0.860)8 (0.030) 8/266 12.0 3.0% coding (2540/2841 nt) glnE fused deadenylyltransferase/adenylyltransferase for glutamine synthetase
?AP012306 2670451 = 253 (0.840)coding (2524/2841 nt) glnE fused deadenylyltransferase/adenylyltransferase for glutamine synthetase
* ? AP012306 544338 =333 (0.990)9 (0.030) 9/292 12.1 2.7% coding (784/1032 nt) holA DNA polymerase III, delta subunit
?AP012306 544358 = 326 (0.990)coding (764/1032 nt) holA DNA polymerase III, delta subunit
* ? AP012306 = 1092334302 (0.900)7 (0.020) 7/258 12.3 2.6% coding (198/276 nt) ychS predicted protein
?AP012306 1092985 = 268 (0.920)noncoding (66/85 nt) tyrT tRNA‑Tyr