breseq version 0.35.4 revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log |
Marginal read alignment evidence (highest frequency 20 of 29 shown, sorted by frequency from high to low) | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | AP012306 | 1,091,992 | 0 | T | G | 39.3% | 427.5 / 69.0 | 253 | intergenic (+38/‑145) | narI/ychS | nitrate reductase 1, gamma (cytochrome b(NR)) subunit/predicted protein |
* | AP012306 | 1,944,288 | 0 | A | C | 37.6% | 331.3 / 53.9 | 202 | G361G (GGT→GGG) | menE | o‑succinylbenzoate‑CoA ligase |
* | AP012306 | 644,417 | 0 | G | A | 31.8% | 908.9 / inf | 607 | intergenic (+32/‑401) | lysQ/nadA | tRNA‑Lys/quinolinate synthase, subunit A |
* | AP012306 | 1,309,914 | 0 | A | C | 31.6% | 263.7 / 10.2 | 136 | L23F (TTA→TTC) | lsrC | AI2 transporter |
* | AP012306 | 3,938,887 | 0 | C | T | 31.5% | 480.0 / 185.2 | 276 | S44F (TCT→TTT) | bglJ | DNA‑binding transcriptional regulator |
* | AP012306 | 800,675 | 0 | A | C | 30.6% | 547.2 / 44.7 | 262 | T192P (ACC→CCC) | lolA | chaperone for lipoproteins |
* | AP012306 | 935,054 | 0 | G | T | 29.7% | 148.2 / 35.3 | 64 | intergenic (+24/‑1406) | putP/ycdO | proline:sodium symporter/conserved protein |
* | AP012306 | 2,758,909 | 0 | T | G | 29.3% | 608.4 / 35.1 | 267 | G33G (GGT→GGG) | agaD | N‑acetylgalactosamine‑specific enzyme IID component of PTS |
* | AP012306 | 202,417 | 0 | T | G | 28.5% | 598.8 / 39.0 | 271 | V409G (GTA→GGA) | tilS | tRNA(Ile)‑lysidine synthetase |
* | AP012306 | 341,377 | 0 | T | G | 27.4% | 203.3 / 17.2 | 106 | G60G (GGT→GGG) | ribD | fused diaminohydroxyphosphoribosylaminopyrimidin e deaminase and 5‑amino‑6‑(5‑phosphoribosylamino) uracil reductase |
* | AP012306 | 3,527,368 | 0 | A | C | 26.9% | 738.8 / 54.7 | 305 | L140F (TTA→TTC) | rhaS | DNA‑binding transcriptional activator, L‑rhamnose‑binding |
* | AP012306 | 3,903,791 | 0 | A | G | 24.6% | 679.0 / 76.8 | 285 | E189G (GAA→GGA) | yjgJ | predicted transcriptional regulator |
* | AP012306 | 1,128,412 | 0 | T | G | 24.2% | 670.3 / 68.2 | 289 | V29G (GTG→GGG) | yciO | conserved protein |
* | AP012306 | 406,677 | 0 | A | G | 24.0% | 552.1 / 24.7 | 221 | K294E (AAA→GAA) | hemH | ferrochelatase |
* | AP012306 | 2,857,911 | 0 | A | C | 23.7% | 518.8 / 12.1 | 198 | K180N (AAA→AAC) | aaeR | predicted DNA‑binding transcriptional regulator, efflux system |
* | AP012306 | 1,854,851 | 0 | A | C | 23.3% | 773.9 / 62.1 | 309 | T25P (ACC→CCC) | yejH | predicted ATP‑dependet helicase |
* | AP012306 | 1,066,323 | 0 | A | C | 23.2% | 763.7 / 18.8 | 280 | intergenic (‑92/+33) | ychM/prsA | predicted transporter/phosphoribosylpyrophosphate synthase |
* | AP012306 | 2,212,753 | 0 | A | C | 23.1% | 339.7 / 34.4 | 134 | Y241D (TAT→GAT) | hcaT | predicted 3‑phenylpropionic transporter |
* | AP012306 | 2,558,071 | 0 | A | C | 22.7% | 590.4 / 32.4 | 229 | V302G (GTA→GGA) | visC | predicted oxidoreductase, FAD/NAD(P)‑binding domain |
* | AP012306 | 3,282,076 | 0 | T | G | 22.7% | 591.7 / 12.0 | 252 | intergenic (‑79/+27) | ilvB/ivbL | acetolactate synthase I, large subunit/ilvB operon leader peptide |
Marginal new junction evidence (lowest skew 10 of 283 shown) | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | reads (cov) | reads (cov) | score | skew | freq | annotation | gene | product | ||
* | ? | AP012306 | = 2275012 | 305 (0.910) | 14 (0.050) | 14/274 | 10.4 | 4.8% | noncoding (60/76 nt) | gltW | tRNA‑Glu |
? | AP012306 | 3372719 = | NA (NA) | noncoding (72/76 nt) | gltU | tRNA‑Glu | |||||
* | ? | AP012306 | 644310 = | 439 (1.310) | 35 (0.110) +GAG |
14/292 | 10.7 | 8.2% | noncoding (1/76 nt) | lysQ | tRNA‑Lys |
? | AP012306 | = 2073420 | 364 (1.080) | noncoding (76/76 nt) | valU | tRNA‑Val | |||||
* | ? | AP012306 | = 1092512 | 346 (1.030) | 13 (0.040) | 11/258 | 10.9 | 4.4% | intergenic (+100/+3) | ychS/tpr | predicted protein/predicted protamine‑like protein |
? | AP012306 | 1092985 = | 268 (0.920) | noncoding (66/85 nt) | tyrT | tRNA‑Tyr | |||||
* | ? | AP012306 | 3597644 = | 242 (0.720) | 11 (0.040) | 11/274 | 11.2 | 4.7% | noncoding (60/76 nt) | gltT | tRNA‑Glu |
? | AP012306 | 3639058 = | NA (NA) | noncoding (72/76 nt) | gltV | tRNA‑Glu | |||||
* | ? | AP012306 | = 2275012 | 305 (0.910) | 11 (0.040) | 11/274 | 11.2 | 3.6% | noncoding (60/76 nt) | gltW | tRNA‑Glu |
? | AP012306 | = 2275000 | 303 (0.980) | noncoding (72/76 nt) | gltW | tRNA‑Glu | |||||
* | ? | AP012306 | 644310 = | 439 (1.310) | 32 (0.100) +G |
12/296 | 11.3 | 7.5% | noncoding (1/76 nt) | lysQ | tRNA‑Lys |
? | AP012306 | = 2073542 | 351 (1.040) | intergenic (+2/‑45) | valX/valY | tRNA‑Val/tRNA‑Val | |||||
* | ? | AP012306 | = 2275012 | 305 (0.910) | 9 (0.030) | 9/274 | 11.8 | 3.3% | noncoding (60/76 nt) | gltW | tRNA‑Glu |
? | AP012306 | 3597656 = | 242 (0.780) | noncoding (72/76 nt) | gltT | tRNA‑Glu | |||||
* | ? | AP012306 | 2670435 = | 288 (0.860) | 8 (0.030) | 8/266 | 12.0 | 3.0% | coding (2540/2841 nt) | glnE | fused deadenylyltransferase/adenylyltransferase for glutamine synthetase |
? | AP012306 | 2670451 = | 253 (0.840) | coding (2524/2841 nt) | glnE | fused deadenylyltransferase/adenylyltransferase for glutamine synthetase | |||||
* | ? | AP012306 | 544338 = | 333 (0.990) | 9 (0.030) | 9/292 | 12.1 | 2.7% | coding (784/1032 nt) | holA | DNA polymerase III, delta subunit |
? | AP012306 | 544358 = | 326 (0.990) | coding (764/1032 nt) | holA | DNA polymerase III, delta subunit | |||||
* | ? | AP012306 | = 1092334 | 302 (0.900) | 7 (0.020) | 7/258 | 12.3 | 2.6% | coding (198/276 nt) | ychS | predicted protein |
? | AP012306 | 1092985 = | 268 (0.920) | noncoding (66/85 nt) | tyrT | tRNA‑Tyr |