breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence seq id position mutation freq annotation gene description
RA NZ_CP022686 119,184 T→G 10.7% F47V (TTT→GTT)  marB → multiple antibiotic resistance protein MarB
RA NZ_CP022686 126,825 G→T 9.5% G64C (GGT→TGT)  ydeE → efflux MFS transporter YdeE
RA NZ_CP022686 177,506 T→G 6.3% Q140H (CAA→CAC uidB ← glucuronide transporter
RA NZ_CP022686 182,634 A→C 11.4% R72R (CGT→CGG malI ← mal regulon transcriptional regulator MalI
RA NZ_CP022686 192,380 T→A 9.5% R597R (CGT→CGA rsxC → electron transport complex subunit RsxC
JC NZ_CP022686 235,231 Δ83 bp 15.7% coding (245‑327/1413 nt) pykF → pyruvate kinase PykF
RA NZ_CP022686 235,926 G→A 7.7% E314K (GAA→AAA)  pykF → pyruvate kinase PykF
RA NZ_CP022686 340,516 A→C 12.8% F31V (TTT→GTT)  ydjK ← MFS transporter
RA NZ_CP022686 349,106 A→C 8.8% T607P (ACC→CCC)  yeaG → protein kinase YeaG
RA NZ_CP022686 360,290 T→G 9.0% T14P (ACG→CCG)  yeaR ← DUF1971 domain‑containing protein YeaR
RA NZ_CP022686 372,541 T→A 6.9% V264E (GTA→GAA)  sdaA → L‑serine ammonia‑lyase
RA NZ_CP022686 372,574 A→C 17.3% N275T (AAC→ACC)  sdaA → L‑serine ammonia‑lyase
RA NZ_CP022686 436,591 A→C 7.3% intergenic (‑19/+38) CIW80_RS02305 ← / ← CIW80_RS02310 phage minor tail protein L/phage tail protein
RA NZ_CP022686 457,894 Δ1 bp 100% pseudogene (288/997 nt) CIW80_RS02445 ← DUF968 domain‑containing protein
RA NZ_CP022686 531,408 A→C 11.7% K44N (AAA→AAC fliF → flagellar basal‑body MS‑ring/collar protein FliF
RA NZ_CP022686 556,942 G→T 24.0% intergenic (+126/‑722) zinT → / → CIW80_RS03080 metal‑binding protein ZinT/site‑specific integrase
RA NZ_CP022686 556,949 G→T 14.2% intergenic (+133/‑715) zinT → / → CIW80_RS03080 metal‑binding protein ZinT/site‑specific integrase
RA NZ_CP022686 556,957 C→A 15.8% intergenic (+141/‑707) zinT → / → CIW80_RS03080 metal‑binding protein ZinT/site‑specific integrase
RA NZ_CP022686 560,880 C→T 100% intergenic (+62/+53) CIW80_RS03085 → / ← pilV hypothetical protein/shufflon system plasmid conjugative transfer pilus tip adhesin PilV
MC JC NZ_CP022686 628,478 Δ1,286 bp 100% [clbP] [clbP]
RA NZ_CP022686 651,848 A→C 12.6% R238R (CGT→CGG clbJ ← colibactin non‑ribosomal peptide synthetase ClbJ
RA NZ_CP022686 694,391 C→A 10.8% intergenic (+151/‑45) CIW80_RS03555 → / → CIW80_RS03560 transposase/PfkB family carbohydrate kinase
RA NZ_CP022686 694,402 G→T 11.6% intergenic (+162/‑34) CIW80_RS03555 → / → CIW80_RS03560 transposase/PfkB family carbohydrate kinase
RA NZ_CP022686 756,115 A→G 100% G181G (GGT→GGC galE ← UDP‑glucose 4‑epimerase GalE
RA NZ_CP022686 764,997 T→G 16.0% N41T (AAC→ACC)  wcaM ← colanic acid biosynthesis protein WcaM
RA NZ_CP022686 765,008 A→T 9.1% I37I (ATT→ATA wcaM ← colanic acid biosynthesis protein WcaM
RA NZ_CP022686 794,460 C→T 100% A568V (GCG→GTG)  dgcE → diguanylate cyclase
RA NZ_CP022686 828,846 A→C 12.2% L189R (CTT→CGT)  gatY ← tagatose‑bisphosphate aldolase subunit GatY
RA NZ_CP022686 829,441 C→A 19.8% intergenic (‑30/+197) gatY ← / ← fbaB tagatose‑bisphosphate aldolase subunit GatY/class I fructose‑bisphosphate aldolase
RA NZ_CP022686 829,452 G→T 23.4% intergenic (‑41/+186) gatY ← / ← fbaB tagatose‑bisphosphate aldolase subunit GatY/class I fructose‑bisphosphate aldolase
RA NZ_CP022686 829,467 C→A 18.2% intergenic (‑56/+171) gatY ← / ← fbaB tagatose‑bisphosphate aldolase subunit GatY/class I fructose‑bisphosphate aldolase
RA NZ_CP022686 829,472 G→T 13.8% intergenic (‑61/+166) gatY ← / ← fbaB tagatose‑bisphosphate aldolase subunit GatY/class I fructose‑bisphosphate aldolase
RA NZ_CP022686 859,943 T→G 12.4% L602V (TTA→GTA)  yehM → DUF5682 family protein
RA NZ_CP022686 881,745 C→G 10.1% intergenic (+260/+127) yohP → / ← dusC protein YohP/tRNA dihydrouridine(16) synthase DusC
RA NZ_CP022686 917,427:1 +TT 100% intergenic (+130/‑91) yeiP → / → yeiQ elongation factor P‑like protein YeiP/mannitol dehydrogenase family protein
RA NZ_CP022686 948,027 A→T 11.3% intergenic (+96/‑161) CIW80_RS04855 → / → CIW80_RS04860 terminase small subunit/hypothetical protein
RA NZ_CP022686 948,029 C→A 14.5% intergenic (+98/‑159) CIW80_RS04855 → / → CIW80_RS04860 terminase small subunit/hypothetical protein
RA NZ_CP022686 948,046 G→T 12.1% intergenic (+115/‑142) CIW80_RS04855 → / → CIW80_RS04860 terminase small subunit/hypothetical protein
RA NZ_CP022686 948,049 A→T 10.7% intergenic (+118/‑139) CIW80_RS04855 → / → CIW80_RS04860 terminase small subunit/hypothetical protein
RA NZ_CP022686 948,051 G→T 10.5% intergenic (+120/‑137) CIW80_RS04855 → / → CIW80_RS04860 terminase small subunit/hypothetical protein
RA NZ_CP022686 948,080 G→T 13.7% intergenic (+149/‑108) CIW80_RS04855 → / → CIW80_RS04860 terminase small subunit/hypothetical protein
RA NZ_CP022686 948,090 C→A 16.5% intergenic (+159/‑98) CIW80_RS04855 → / → CIW80_RS04860 terminase small subunit/hypothetical protein
RA NZ_CP022686 954,719 C→A 8.1% E132* (GAA→TAA)  ccmE ← cytochrome c maturation protein CcmE
RA NZ_CP022686 957,112 T→G 13.4% T89P (ACC→CCC)  ccmA ← cytochrome c biogenesis heme‑transporting ATPase CcmA
RA NZ_CP022686 1,005,085 C→A 11.0% T380T (ACC→ACA nrdA → ribonucleoside‑diphosphate reductase subunit alpha
RA NZ_CP022686 1,011,397 T→A 9.6% intergenic (‑246/‑27) glpT ← / → glpA glycerol‑3‑phosphate transporter/anaerobic glycerol‑3‑phosphate dehydrogenase subunit A
RA NZ_CP022686 1,034,493 T→G 7.7% T26P (ACC→CCC)  menC ← o‑succinylbenzoate synthase
RA NZ_CP022686 1,047,906 G→T 9.4% intergenic (‑136/+28) nuoM ← / ← nuoL NADH‑quinone oxidoreductase subunit M/NADH‑quinone oxidoreductase subunit L
RA NZ_CP022686 1,100,907 T→G 16.0% E199D (GAA→GAC aroC ← chorismate synthase
RA NZ_CP022686 1,100,959 T→G 11.8% D182A (GAC→GCC)  aroC ← chorismate synthase
RA NZ_CP022686 1,174,272 A→C 16.0% intergenic (+1/‑43) CIW80_RS05855 → / → CIW80_RS05860 tRNA‑Val/tRNA‑Val
RA NZ_CP022686 1,174,416 A→T 9.2% intergenic (+26/‑21) CIW80_RS05860 → / → CIW80_RS05865 tRNA‑Val/tRNA‑Val
RA NZ_CP022686 1,209,917 G→T 13.0% P393Q (CCA→CAA)  eutH ← ethanolamine utilization protein EutH
RA NZ_CP022686 1,213,479 T→G 21.4% G420G (GGA→GGC eutE ← aldehyde dehydrogenase EutE
RA NZ_CP022686 1,237,918 C→A 11.3% C303F (TGC→TTC)  tmcA ← tRNA cytosine(34) acetyltransferase TmcA
RA NZ_CP022686 1,325,398 C→A 7.6% E260* (GAA→TAA)  qseE ← two component system sensor histidine kinase QseE/GlrK
RA NZ_CP022686 1,360,709 T→G 14.6% intergenic (‑44/+280) kgtP ← / ← rrf alpha‑ketoglutarate permease/5S ribosomal RNA
RA NZ_CP022686 1,360,724 G→T 17.5% intergenic (‑59/+265) kgtP ← / ← rrf alpha‑ketoglutarate permease/5S ribosomal RNA
RA NZ_CP022686 1,360,736 G→T 8.9% intergenic (‑71/+253) kgtP ← / ← rrf alpha‑ketoglutarate permease/5S ribosomal RNA
RA NZ_CP022686 1,360,756 C→A 12.6% intergenic (‑91/+233) kgtP ← / ← rrf alpha‑ketoglutarate permease/5S ribosomal RNA
RA NZ_CP022686 1,360,762 G→T 7.0% intergenic (‑97/+227) kgtP ← / ← rrf alpha‑ketoglutarate permease/5S ribosomal RNA
RA NZ_CP022686 1,447,975 C→A 7.0% Q165H (CAG→CAT hycG ← formate hydrogenlyase subunit HycG
RA NZ_CP022686 1,460,615 A→C 12.5% K673N (AAA→AAC flhA → formate hydrogenlyase transcriptional activator FlhA
RA NZ_CP022686 1,479,607 A→C 13.5% intergenic (‑16/+177) ftsB ← / ← ygbE cell division protein FtsB/DUF3561 family protein
RA NZ_CP022686 1,479,619 T→A 14.2% intergenic (‑28/+165) ftsB ← / ← ygbE cell division protein FtsB/DUF3561 family protein
RA NZ_CP022686 1,504,305 A→T 8.3% N248Y (AAT→TAT)  CIW80_RS07520 → YgcG family protein
RA NZ_CP022686 1,504,310 G→T 13.3% M249I (ATG→ATT CIW80_RS07520 → YgcG family protein
RA NZ_CP022686 1,547,110 G→C 16.4% intergenic (+22/‑12) CIW80_RS07710 → / → CIW80_RS07715 tRNA‑Met/tRNA‑Met
RA NZ_CP022686 1,579,312 A→C 11.1% V107V (GTT→GTG CIW80_RS07835 ← PRD domain‑containing protein
RA NZ_CP022686 1,604,828 A→C 8.3% L390R (CTG→CGG)  lplT ← lysophospholipid transporter LplT
RA NZ_CP022686 1,632,501 T→G 12.8% V166G (GTT→GGT)  arcC → carbamate kinase
RA NZ_CP022686 1,633,846 T→G 31.9% N253T (AAC→ACC)  yqeB ← selenium‑dependent molybdenum cofactor biosynthesis protein YqeB
RA NZ_CP022686 1,640,925 A→C 11.1% R7S (AGA→AGC ygfM → molybdopterin‑dependent oxidoreductase FAD‑binding subunit
RA NZ_CP022686 1,674,224 T→G 14.3% K188N (AAA→AAC ygfB ← UPF0149 family protein YgfB
RA NZ_CP022686 1,674,231 A→T 9.9% V186E (GTA→GAA)  ygfB ← UPF0149 family protein YgfB
RA NZ_CP022686 1,674,244 T→G 14.4% T182P (ACC→CCC)  ygfB ← UPF0149 family protein YgfB
RA NZ_CP022686 1,674,248 T→G 11.1% Q180H (CAA→CAC ygfB ← UPF0149 family protein YgfB
RA NZ_CP022686 1,706,047 G→T 11.0% M1M (ATG→ATT) † metK → methionine adenosyltransferase
RA NZ_CP022686 1,716,145 A→C 8.3% T42P (ACC→CCC)  rdgB → XTP/dITP diphosphatase
RA NZ_CP022686 1,716,167 A→C 18.8% N49T (AAC→ACC)  rdgB → XTP/dITP diphosphatase
RA NZ_CP022686 1,716,202 A→C 9.6% T61P (ACC→CCC)  rdgB → XTP/dITP diphosphatase
RA NZ_CP022686 1,745,268 A→C 5.2% L183R (CTG→CGG)  CIW80_RS08660 ← MFS transporter
RA NZ_CP022686 1,775,833 T→G 7.1% N202T (AAC→ACC)  CIW80_RS08815 ← N‑acetylneuraminate epimerase
RA NZ_CP022686 1,775,845 T→G 8.1% N198T (AAC→ACC)  CIW80_RS08815 ← N‑acetylneuraminate epimerase
RA NZ_CP022686 1,785,695 T→G 10.9% pseudogene (16/186 nt) CIW80_RS08875 ← zinc‑ribbon domain‑containing protein
RA NZ_CP022686 1,833,762 A→C 12.9% L462R (CTG→CGG)  gspD ← type II secretion system secretin GspD
RA NZ_CP022686 1,888,356 C→A 12.2% S239* (TCA→TAA)  yqhG → YbjP/YqhG family protein
RA NZ_CP022686 1,888,373 C→A 12.7% R245R (CGA→AGA)  yqhG → YbjP/YqhG family protein
RA NZ_CP022686 1,911,602 T→G 10.1% N224T (AAC→ACC)  CIW80_RS09520 ← MurR/RpiR family transcriptional regulator
RA NZ_CP022686 1,925,365 A→C 5.3% D162A (GAC→GCC)  ygiB → DUF1190 family protein
RA NZ_CP022686 1,954,923 T→G 16.9% T92P (ACG→CCG)  ttdR ← DNA‑binding transcriptional activator TtdR
RA NZ_CP022686 2,016,160 T→G 5.2% noncoding (114/377 nt) rnpB ← RNase P RNA component class A
RA NZ_CP022686 2,016,170 A→C 7.1% noncoding (104/377 nt) rnpB ← RNase P RNA component class A
RA NZ_CP022686 2,027,687 T→G 6.3% R179R (CGT→CGG agaE → PTS N‑acetylgalactosamine transporter subunit IID
RA NZ_CP022686 2,049,089 T→G 16.0% N256T (AAC→ACC)  deaD ← ATP‑dependent RNA helicase DeaD
RA NZ_CP022686 2,049,094 G→T 11.1% T254T (ACC→ACA deaD ← ATP‑dependent RNA helicase DeaD
RA NZ_CP022686 2,056,193 A→C 6.4% V606G (GTT→GGT)  infB ← translation initiation factor IF‑2
RA NZ_CP022686 2,061,780 A→C 7.8% K383N (AAA→AAC argG → argininosuccinate synthase
RA NZ_CP022686 2,070,788 A→C 6.6% intergenic (‑80/‑169) greA ← / → dacB transcription elongation factor GreA/serine‑type D‑Ala‑D‑Ala carboxypeptidase
RA NZ_CP022686 2,076,552 A→C 7.9% intergenic (+22/‑211) ispB → / → sfsB octaprenyl diphosphate synthase/DNA‑binding transcriptional regulator SfsB
RA NZ_CP022686 2,076,554 T→G 9.3% intergenic (+24/‑209) ispB → / → sfsB octaprenyl diphosphate synthase/DNA‑binding transcriptional regulator SfsB
RA NZ_CP022686 2,083,926 T→G 7.3% R269R (CGT→CGG kdsD → arabinose‑5‑phosphate isomerase KdsD
RA NZ_CP022686 2,110,457 T→G 15.3% K12N (AAA→AAC rplM ← 50S ribosomal protein L13
RA NZ_CP022686 2,110,465 T→G 9.9% T10P (ACC→CCC)  rplM ← 50S ribosomal protein L13
RA NZ_CP022686 2,123,241 A→C 9.4% F208V (TTT→GTT)  tldD ← metalloprotease TldD
RA NZ_CP022686 2,130,829 A→C 7.5% V237G (GTT→GGT)  mreC ← rod shape‑determining protein MreC
RA NZ_CP022686 2,148,102 C→G 27.7% L122V (CTT→GTT)  panF → sodium/pantothenate symporter
RA NZ_CP022686 2,148,109 T→G 9.2% V124G (GTG→GGG)  panF → sodium/pantothenate symporter
RA NZ_CP022686 2,148,115 T→G 15.1% L126R (CTG→CGG)  panF → sodium/pantothenate symporter
RA NZ_CP022686 2,148,136 T→G 12.3% V133G (GTT→GGT)  panF → sodium/pantothenate symporter
RA NZ_CP022686 2,158,627 A→C 5.7% T36P (ACC→CCC)  CIW80_RS10770 → membrane protein
RA NZ_CP022686 2,185,075 A→C 9.8% V46G (GTT→GGT)  rpsE ← 30S ribosomal protein S5
RA NZ_CP022686 2,188,239 G→T 8.1% intergenic (‑108/+57) rplN ← / ← rpsQ 50S ribosomal protein L14/30S ribosomal protein S17
RA NZ_CP022686 2,207,097 G→C 8.5% intergenic (‑121/+48) bfd ← / ← chiA bacterioferritin‑associated ferredoxin/bifunctional chitinase/lysozyme
RA NZ_CP022686 2,207,100 C→A 7.8% intergenic (‑124/+45) bfd ← / ← chiA bacterioferritin‑associated ferredoxin/bifunctional chitinase/lysozyme
RA NZ_CP022686 2,214,419 T→C 100% K43R (AAA→AGA)  rpsL ← 30S ribosomal protein S12
RA NZ_CP022686 2,226,139 A→C 6.3% D9A (GAC→GCC)  crp → cAMP‑activated global transcriptional regulator CRP
RA NZ_CP022686 2,320,775 C→T 100% G274S (GGC→AGC)  CIW80_RS11675 ← DNA‑processing protein DprA
RA NZ_CP022686 2,361,489 T→G 10.8% V188G (GTT→GGT)  nikA → nickel ABC transporter substrate‑binding protein
RA NZ_CP022686 2,361,501 C→A 8.5% S192* (TCG→TAG)  nikA → nickel ABC transporter substrate‑binding protein
RA NZ_CP022686 2,362,972 T→G 8.4% F158V (TTT→GTT)  nikB → nickel ABC transporter permease subunit NikB
RA NZ_CP022686 2,386,496 T→G 9.2% K417N (AAA→AAC prlC ← oligopeptidase A
RA NZ_CP022686 2,393,069 C→A 5.2% L19M (CTG→ATG)  dctR → LuxR C‑terminal‑related transcriptional regulator
RA NZ_CP022686 2,398,836 G→T 7.3% K186N (AAG→AAT hutW → heme anaerobic degradation radical SAM methyltransferase ChuW/HutW
RA NZ_CP022686 2,398,896 A→C 15.9% K206N (AAA→AAC hutW → heme anaerobic degradation radical SAM methyltransferase ChuW/HutW
RA NZ_CP022686 2,408,861 Δ1 bp 100% pseudogene (1097/3115 nt) mdtF → multidrug efflux pump RND permease MdtF
RA NZ_CP022686 2,429,953 A→C 7.5% R275R (CGT→CGG dctA ← C4‑dicarboxylate transporter DctC
RA NZ_CP022686 2,460,526 A→C 11.5% V18G (GTG→GGG)  yhjY ← autotransporter outer membrane beta‑barrel domain‑containing protein
RA NZ_CP022686 2,460,576 C→A 9.1% M1M (ATG→ATT) † yhjY ← autotransporter outer membrane beta‑barrel domain‑containing protein
RA NZ_CP022686 2,460,579 T→A 24.2% intergenic (‑1/‑157) yhjY ← / → tag autotransporter outer membrane beta‑barrel domain‑containing protein/DNA‑3‑methyladenine glycosylase I
RA NZ_CP022686 2,470,422 T→G 8.7% K72N (AAA→AAC glyS ← glycine‑‑tRNA ligase subunit beta
RA NZ_CP022686 2,516,577 A→G 5.5% T663A (ACA→GCA)  upaG → trimeric autotransporter adhesin UpaG
RA NZ_CP022686 2,516,624 G→C 6.2% K678N (AAG→AAC upaG → trimeric autotransporter adhesin UpaG
RA NZ_CP022686 2,516,969 C→A 11.8% T793T (ACC→ACA upaG → trimeric autotransporter adhesin UpaG
JC NZ_CP022686 2,542,904 +ATGCC 11.2% coding (811/996 nt) CIW80_RS12735 ← glycosyltransferase
RA NZ_CP022686 2,546,394 A→C 13.2% V95G (GTT→GGT)  waaG ← glycosyltransferase family 4 protein
RA NZ_CP022686 2,546,416 T→A 8.8% N88Y (AAT→TAT)  waaG ← glycosyltransferase family 4 protein
RA NZ_CP022686 2,574,429 T→G 9.0% N230T (AAC→ACC)  CIW80_RS12895 ← class II fructose‑bisphosphate aldolase family protein
JC NZ_CP022686 2,590,563 +TTGG 100% coding (109/153 nt) CIW80_RS26455 → hypothetical protein
RA NZ_CP022686 2,632,806 G→T 7.3% T64N (ACC→AAC)  dgoT ← MFS transporter
RA NZ_CP022686 2,632,838 A→C 8.1% A53A (GCT→GCG dgoT ← MFS transporter
RA NZ_CP022686 2,638,855 C→A 9.6% intergenic (‑170/+70) yidB ← / ← gyrB YidB family protein/DNA topoisomerase (ATP‑hydrolyzing) subunit B
RA NZ_CP022686 2,703,299 A→C 11.1% intergenic (‑203/‑277) yieP ← / → CIW80_RS13540 FadR/GntR family transcriptional regulator/16S ribosomal RNA
RA NZ_CP022686 2,718,641 T→G 6.8% G514G (GGT→GGG
*297Y (TAA→TAC
ilvA →
ilvY ←
threonine ammonia‑lyase, biosynthetic
HTH‑type transcriptional activator IlvY
RA NZ_CP022686 2,736,585 T→A 12.2% Y183N (TAC→AAC)  rffA → dTDP‑4‑amino‑4,6‑dideoxygalactose transaminase
RA NZ_CP022686 2,743,563 T→G 13.9% noncoding (65/87 nt) CIW80_RS13750 → tRNA‑Leu
RA NZ_CP022686 2,758,128 A→C 6.5% K40N (AAA→AAC yifL → lipoprotein
RA NZ_CP022686 2,761,539 A→C 8.6% N24H (AAC→CAC)  uvrD → DNA helicase II
RA NZ_CP022686 2,761,551 C→G 6.4% L28V (CTG→GTG)  uvrD → DNA helicase II
RA NZ_CP022686 2,769,166 C→A 11.3% C57F (TGC→TTC)  rarD ← EamA family transporter RarD
RA NZ_CP022686 2,801,817 T→G 5.1% Y32S (TAC→TCC)  CIW80_RS14030 ← 2‑dehydro‑3‑deoxy‑6‑phosphogalactonate aldolase
RA NZ_CP022686 2,801,864 T→G 5.6% L16F (TTA→TTC CIW80_RS14030 ← 2‑dehydro‑3‑deoxy‑6‑phosphogalactonate aldolase
RA NZ_CP022686 2,821,644 A→C 7.7% intergenic (+100/‑278) hemG → / → CIW80_RS14130 menaquinone‑dependent protoporphyrinogen IX dehydrogenase/16S ribosomal RNA
RA NZ_CP022686 2,834,898 A→C 6.7% K601N (AAA→AAC polA → DNA polymerase I
RA NZ_CP022686 2,868,740 T→G 23.1% Q22P (CAG→CCG)  fdoH ← formate dehydrogenase O subunit beta
RA NZ_CP022686 2,868,753 T→G 12.3% T18P (ACC→CCC)  fdoH ← formate dehydrogenase O subunit beta
RA NZ_CP022686 2,871,422 T→G 7.8% N149T (AAC→ACC)  fdnG ← formate dehydrogenase‑N subunit alpha
RA NZ_CP022686 2,871,457 C→A 7.4% M137I (ATG→ATT fdnG ← formate dehydrogenase‑N subunit alpha
RA NZ_CP022686 2,904,856 T→G 6.5% K237N (AAA→AAC ftsN ← cell division protein FtsN
RA NZ_CP022686 2,981,597 G→T 12.0% E562* (GAA→TAA)  rpoC → DNA‑directed RNA polymerase subunit beta'
RA NZ_CP022686 2,989,627 T→G 6.6% Q39H (CAA→CAC thiC ← phosphomethylpyrimidine synthase ThiC
RA NZ_CP022686 3,017,622 A→C 14.2% K839N (AAA→AAC metH → methionine synthase
RA NZ_CP022686 3,020,229 T→G 13.1% V133G (GTC→GGC)  yjbB → Na/Pi cotransporter family protein
RA NZ_CP022686 3,052,316 T→G 6.2% intergenic (+38/‑35) lexA → / → dinF transcriptional repressor LexA/MATE family efflux transporter DinF
RA NZ_CP022686 3,086,834 A→C 7.7% F708V (TTT→GTT)  uvrA ← excinuclease ABC subunit UvrA
RA NZ_CP022686 3,090,033 G→T 7.0% R32S (CGT→AGT)  yjcB ← YjcB family protein
RA NZ_CP022686 3,090,101 A→C 10.6% V9G (GTT→GGT)  yjcB ← YjcB family protein
RA NZ_CP022686 3,136,940 T→G 8.5% D161A (GAC→GCC)  phnN ← ribose 1,5‑bisphosphokinase
RA NZ_CP022686 3,184,651 A→C 7.4% intergenic (‑98/+18) cutA ← / ← dcuA divalent cation tolerance protein CutA/anaerobic C4‑dicarboxylate transporter DcuA
JC NZ_CP022686 3,196,873 +GCGTAATCGGTT 100% coding (944/1122 nt) ampC ← BlaEC family class C beta‑lactamase
RA NZ_CP022686 3,213,819 T→G 7.7% R479R (CGT→CGG nnr → bifunctional ADP‑dependent NAD(P)H‑hydrate dehydratase/NAD(P)H‑hydrate epimerase
RA NZ_CP022686 3,252,190 T→G 12.7% K221N (AAA→AAC CIW80_RS16225 ← enoyl‑CoA hydratase/isomerase family protein
RA NZ_CP022686 3,333,817 C→T 11.2% E89K (GAG→AAG)  fecE ← Fe(3+) dicitrate ABC transporter ATP‑binding protein FecE
RA NZ_CP022686 3,334,291 C→A 6.8% G250C (GGT→TGT)  fecD ← Fe(3+) dicitrate ABC transporter permease subunit FecD
RA NZ_CP022686 3,345,072 T→G 8.8% K111T (AAG→ACG)  CIW80_RS16655 ← IS66 family transposase
RA NZ_CP022686 3,348,342 A→T 6.6% intergenic (‑158/‑42) CIW80_RS16685 ← / → CIW80_RS26495 inovirus Gp2 family protein/hypothetical protein
RA NZ_CP022686 3,348,371 T→G 6.4% intergenic (‑187/‑13) CIW80_RS16685 ← / → CIW80_RS26495 inovirus Gp2 family protein/hypothetical protein
RA NZ_CP022686 3,357,439 G→T 6.8% G8C (GGT→TGT)  CIW80_RS16765 → DUF932 domain‑containing protein
RA NZ_CP022686 3,390,605 T→G 17.5% K146N (AAA→AAC sgcX ← M42 family metallopeptidase
RA NZ_CP022686 3,390,629 C→A 11.9% W138C (TGG→TGT sgcX ← M42 family metallopeptidase
RA NZ_CP022686 3,424,795 A→C 100% intergenic (‑41/+27) yjiJ ← / ← yjiK MFS transporter/YjiK family protein
RA NZ_CP022686 3,444,704 T→G 12.7% T530P (ACC→CCC)  btsT ← pyruvate/proton symporter BtsT
RA NZ_CP022686 3,451,845 A→C 9.0% E278D (GAA→GAC lgoD → L‑galactonate oxidoreductase
RA NZ_CP022686 3,458,194 C→A 5.4% intergenic (‑312/‑309) yjjP ← / → yjjQ threonine/serine exporter ThrE family protein/response regulator transcription factor
RA NZ_CP022686 3,461,127 A→C 9.7% noncoding (65/87 nt) CIW80_RS17335 ← tRNA‑Leu
RA NZ_CP022686 3,461,229 T→G 16.6% noncoding (84/87 nt) CIW80_RS17340 ← tRNA‑Leu
RA NZ_CP022686 3,461,231 C→G 12.2% noncoding (82/87 nt) CIW80_RS17340 ← tRNA‑Leu
RA NZ_CP022686 3,461,247 A→C 14.5% noncoding (66/87 nt) CIW80_RS17340 ← tRNA‑Leu
RA NZ_CP022686 3,469,923 T→G 7.0% E122D (GAA→GAC yjjW ← YjjW family glycine radical enzyme activase
RA NZ_CP022686 3,504,054 T→G 10.7% V204G (GTT→GGT)  tal → transaldolase
RA NZ_CP022686 3,528,218 A→C 7.6% D57A (GAC→GCC)  carB → carbamoyl‑phosphate synthase large subunit
RA NZ_CP022686 3,570,252 T→G 8.5% L223V (TTG→GTG)  yabI → DedA family protein
RA NZ_CP022686 3,652,079 T→G 9.9% intergenic (‑88/+24) panB ← / ← CIW80_RS18255 3‑methyl‑2‑oxobutanoate hydroxymethyltransferase/fimbrial‑like adhesin
RA NZ_CP022686 3,656,188 A→G 100% S549P (TCC→CCC)  htrE ← outer membrane usher protein
RA NZ_CP022686 3,734,367 T→G 19.8% F225V (TTT→GTT)  yafD → endonuclease/exonuclease/phosphatase family protein
RA NZ_CP022686 3,770,508 T→G 10.5% intergenic (‑244/+219) CIW80_RS26265 ← / ← CIW80_RS18890 ATP‑binding protein/hypothetical protein
RA NZ_CP022686 3,770,524 T→G 5.0% intergenic (‑260/+203) CIW80_RS26265 ← / ← CIW80_RS18890 ATP‑binding protein/hypothetical protein
RA NZ_CP022686 3,792,805 A→G 100% Y421C (TAC→TGC)  fucP → L‑fucose:H+ symporter permease
RA NZ_CP022686 3,797,481 T→G 18.2% L130V (TTA→GTA)  CIW80_RS19065 → LacI family DNA‑binding transcriptional regulator
RA NZ_CP022686 3,798,092 C→A 9.1% F333L (TTC→TTA CIW80_RS19065 → LacI family DNA‑binding transcriptional regulator
RA NZ_CP022686 3,850,109 C→A 5.6% intergenic (+7/‑85) gpt → / → frsA xanthine phosphoribosyltransferase/esterase FrsA
RA NZ_CP022686 3,852,459 T→G 11.7% N178T (AAC→ACC)  phoE ← phosphoporin PhoE
RA NZ_CP022686 3,868,857 T→G 8.0% N379T (AAC→ACC)  ecpC ← fimbrial usher EcpC
RA NZ_CP022686 3,909,238 T→G 6.4% D280E (GAT→GAG pdeL → DNA‑binding transcriptional activator/c‑di‑GMP phosphodiesterase PdeL
RA NZ_CP022686 3,924,665 A→C 7.0% intergenic (+2/‑158) prpB → / → prpC methylisocitrate lyase/2‑methylcitrate synthase
RA NZ_CP022686 3,925,282 A→C 9.2% S154R (AGC→CGC)  prpC → 2‑methylcitrate synthase
RA NZ_CP022686 3,925,289 A→C 12.4% N156T (AAC→ACC)  prpC → 2‑methylcitrate synthase
RA NZ_CP022686 3,932,437 T→G 16.3% N186H (AAC→CAC)  lacA ← galactoside O‑acetyltransferase
RA NZ_CP022686 3,935,324 T→G 10.8% N705T (AAC→ACC)  lacZ ← beta‑galactosidase
RA NZ_CP022686 3,940,724 A→C 9.5% V308G (GTA→GGA)  frmA ← S‑(hydroxymethyl)glutathione dehydrogenase
RA NZ_CP022686 3,977,611 A→C 11.1% E73D (GAA→GAC phoR → phosphate regulon sensor histidine kinase PhoR
RA NZ_CP022686 3,977,656 A→C 9.0% K88N (AAA→AAC phoR → phosphate regulon sensor histidine kinase PhoR
RA NZ_CP022686 3,981,933 T→G 14.5% intergenic (+70/‑86) proY → / → malZ proline‑specific permease ProY/maltodextrin glucosidase
RA NZ_CP022686 4,001,654 T→G 8.8% V247G (GTC→GGC)  thiI → tRNA 4‑thiouridine(8) synthase ThiI
RA NZ_CP022686 4,014,238 C→G 17.6% intergenic (+84/‑260) bolA → / → tig transcriptional regulator BolA/trigger factor
RA NZ_CP022686 4,014,250 C→G 7.1% intergenic (+96/‑248) bolA → / → tig transcriptional regulator BolA/trigger factor
RA NZ_CP022686 4,032,292 A→C 13.5% E106D (GAA→GAC glnK → P‑II family nitrogen regulator
RA NZ_CP022686 4,056,962 C→A 13.5% F137L (TTC→TTA adk → adenylate kinase
RA NZ_CP022686 4,079,363 A→C 11.8% A158A (GCT→GCG ybbO ← NADP(+)‑dependent aldehyde reductase
RA NZ_CP022686 4,213,266 A→T 6.6% L190F (TTA→TTT ubiF → 3‑demethoxyubiquinol 3‑hydroxylase
RA NZ_CP022686 4,234,411 C→A 17.0% intergenic (+111/‑331) glnS → / → chiP glutamine‑‑tRNA ligase/chitoporin
RA NZ_CP022686 4,280,095 T→G 5.6% G103G (GGT→GGG cydB → cytochrome d ubiquinol oxidase subunit II
RA NZ_CP022686 4,331,905 A→C 9.6% G300G (GGT→GGG ybhR ← ABC transporter permease
RA NZ_CP022686 4,331,916 G→T 11.5% L297I (CTC→ATC)  ybhR ← ABC transporter permease
RA NZ_CP022686 4,368,341 T→G 14.9% D47A (GAC→GCC)  ybiY ← glycyl‑radical enzyme activating protein
RA NZ_CP022686 4,368,380 T→G 8.8% N34T (AAC→ACC)  ybiY ← glycyl‑radical enzyme activating protein
RA NZ_CP022686 4,440,292 A→C 14.3% Q783H (CAA→CAC ftsK → DNA translocase FtsK
RA NZ_CP022686 4,440,334 A→C 20.9% Q797H (CAA→CAC ftsK → DNA translocase FtsK
RA NZ_CP022686 4,485,614 T→G 9.8% F139V (TTT→GTT)  gloC → hydroxyacylglutathione hydrolase GloC
RA NZ_CP022686 4,508,982 A→C 5.9% N453T (AAC→ACC)  pqiB → intermembrane transport protein PqiB
RA NZ_CP022686 4,605,579 T→G 14.3% T56P (ACG→CCG)  CIW80_RS23010 ← hypothetical protein
RA NZ_CP022686 4,632,182 T→G 6.4% R276R (CGT→CGG mchF → microcin export transporter peptidase/ATP‑binding subunit MchF
RA NZ_CP022686 4,632,188 C→G 6.4% G278G (GGC→GGG mchF → microcin export transporter peptidase/ATP‑binding subunit MchF
RA NZ_CP022686 4,638,424 G→T 7.4% L109L (CTG→CTT CIW80_RS23235 → S/F1C fimbrial major subunit operon transcriptional regulator
RA NZ_CP022686 4,642,938 A→C 5.6% T728P (ACG→CCG)  sfaF → S/F1C fimbrial usher SfaF/FocD
RA NZ_CP022686 4,642,951 A→C 10.1% N732T (AAC→ACC)  sfaF → S/F1C fimbrial usher SfaF/FocD
RA NZ_CP022686 4,764,877 T→G 12.0% intergenic (‑57/‑204) ycfT ← / → lolC acyltransferase family protein/lipoprotein‑releasing ABC transporter permease subunit LolC
RA NZ_CP022686 4,764,880 T→G 10.0% intergenic (‑60/‑201) ycfT ← / → lolC acyltransferase family protein/lipoprotein‑releasing ABC transporter permease subunit LolC
RA NZ_CP022686 4,780,176 A→C 13.1% intergenic (‑75/+95) phoP ← / ← purB two‑component system response regulator PhoP/adenylosuccinate lyase
RA NZ_CP022686 4,783,330 T→G 14.4% E33D (GAA→GAC mnmA ← tRNA 2‑thiouridine(34) synthase MnmA
RA NZ_CP022686 4,814,981 T→G 6.5% W68G (TGG→GGG)  CIW80_RS24295 → phage tail protein
RA NZ_CP022686 4,814,990 T→G 10.5% F71V (TTT→GTT)  CIW80_RS24295 → phage tail protein
RA NZ_CP022686 4,815,886 T→G 13.4% V25G (GTG→GGG)  CIW80_RS24305 → C40 family peptidase
RA NZ_CP022686 4,839,687 T→G 14.0% N144T (AAC→ACC)  minD ← septum site‑determining protein MinD
RA NZ_CP022686 4,870,912 Δ1 bp 100% intergenic (‑108/‑120) dhaK ← / → dhaR dihydroxyacetone kinase subunit DhaK/dihydroxyacetone kinase operon transcriptional regulator DhaR
RA NZ_CP022686 4,905,170 T→G 12.7% V99G (GTT→GGT)  CIW80_RS24815 → coenzyme F390 synthetase
RA NZ_CP022686 4,905,178 T→G 15.6% F102V (TTC→GTC)  CIW80_RS24815 → coenzyme F390 synthetase
RA NZ_CP022686 4,909,243 T→A 5.6% intergenic (‑9/+25) CIW80_RS24840 ← / ← CIW80_RS24845 tRNA‑Tyr/tRNA‑Tyr
RA NZ_CP022686 4,909,251 G→T 6.5% intergenic (‑17/+17) CIW80_RS24840 ← / ← CIW80_RS24845 tRNA‑Tyr/tRNA‑Tyr
RA NZ_CP022686 4,909,263 T→A 6.4% intergenic (‑29/+5) CIW80_RS24840 ← / ← CIW80_RS24845 tRNA‑Tyr/tRNA‑Tyr
RA NZ_CP022686 4,918,873 T→G 5.2% Q378H (CAA→CAC adhE ← bifunctional acetaldehyde‑CoA/alcohol dehydrogenase
RA NZ_CP022686 5,001,164 A→C 20.2% N163T (AAC→ACC)  ompG → monomeric porin OmpG
RA NZ_CP022686 5,042,304 T→C 10.7% N645N (AAT→AAC CIW80_RS25525 → EntS/YbdA MFS transporter
RA NZ_CP022686 5,043,648 G→C 5.2% V1093V (GTG→GTC CIW80_RS25525 → EntS/YbdA MFS transporter
RA NZ_CP022686 5,044,182 T→A 6.3% G1271G (GGT→GGA CIW80_RS25525 → EntS/YbdA MFS transporter
RA pMUT1‑hok‑empty 4,123:1 +T 94.0% intergenic (‑341/–) putative rom (RNA I inhibition modulator protein) ← /  –/–
RA pMUT1‑hok‑empty 4,127 C→A 100% intergenic (‑345/–) putative rom (RNA I inhibition modulator protein) ← /  –/–
RA pMUT1‑hok‑empty 4,132 4 bp→TA 100% intergenic (‑350/–) putative rom (RNA I inhibition modulator protein) ← /  –/–

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NZ_MW240712 338–2618 3068–2957 340–2731 9517 [9484] [8584] 9598 [JNG15_RS00005]–mobA [JNG15_RS00005],JNG15_RS00010,JNG15_RS00015,mobA
* * ÷ pMUT1‑hok‑empty 1257–1394 3987–1721 328–2731 5172 [5155] [4997] 6184 putative HTH domain protein–putative rom (RNA I inhibition modulator protein) putative HTH domain protein,uncharacterized protein,hypothetical protein,putative rom (RNA I inhibition modulator protein)

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NZ_CP022686 = 233585145 (0.620)13 (0.060) 12/248 NT 8.0% coding (180/627 nt) ydhY ferredoxin‑like protein
?NZ_CP022686 236035 = 156 (0.680)coding (1049/1413 nt) pykF pyruvate kinase PykF
* ? NZ_CP022686 3899666 =156 (0.670)139 (0.680) 41/218 NT 51.6% intergenic (‑40/+290) hyxR/ipbA LuxR family transcriptional regulator HyxR/tyrosine‑type DNA invertase IpbA
?NZ_CP022686 3899944 = 125 (0.620)intergenic (‑318/+12) hyxR/ipbA LuxR family transcriptional regulator HyxR/tyrosine‑type DNA invertase IpbA
* ? NZ_CP022686 = 3899681146 (0.630)149 (0.730) 56/218 NT 54.2% intergenic (‑55/+275) hyxR/ipbA LuxR family transcriptional regulator HyxR/tyrosine‑type DNA invertase IpbA
?NZ_CP022686 = 3899927 125 (0.620)intergenic (‑301/+29) hyxR/ipbA LuxR family transcriptional regulator HyxR/tyrosine‑type DNA invertase IpbA
* ? pMUT1‑hok‑empty 456 =3748 (0.440)5493 (0.650) 203/250 NT 67.1% intergenic (–/+1261) –/putative HTH domain protein –/–
?pMUT1‑hok‑empty 4136 = 1648 (0.190)intergenic (‑354/–) putative rom (RNA I inhibition modulator protein)/– –/–
* ? pMUT1‑hok‑empty = 4915612 (0.660)4262 (0.630) 158/200 NT 58.2% intergenic (–/+1226) –/putative HTH domain protein –/–
?pMUT1‑hok‑empty = 4098 1642 (0.240)intergenic (‑316/–) putative rom (RNA I inhibition modulator protein)/– –/–