breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Marginal read alignment evidence (highest frequency 20 of 85 shown, sorted by frequency from high to low)
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1223,440,3480TG57.1% ‑4.8 / 14.6 45A19A (GCT→GCGCJ019_03266hypothetical protein
*ACB1225,974,0740AC57.1% 6.3 / 25.1 39Q688P (CAG→CCG) sasA_19Adaptive‑response sensory‑kinase SasA
*ACB1224,927,8390TG56.0% ‑1.3 / 11.0 27Q192P (CAG→CCG) srp54Signal recognition particle 54 kDa protein
*ACB1222,094,8170TG55.6% ‑3.2 / 16.6 40Q183P (CAG→CCG) CJ019_01995hypothetical protein
*ACB122759,4130TG55.2% 4.3 / 20.6 30intergenic (+22/‑146)mmuM_1/CJ019_00729Homocysteine S‑methyltransferase/L‑methionine gamma‑lyase
*ACB1224,245,1450AC53.3% 12.0 / 34.4 53L178R (CTG→CGG) CJ019_03982Crotonyl‑CoA hydratase
*ACB1221,974,1600TG52.2% 12.2 / 22.3 23intergenic (+42/‑258)rpsA/ihfB30S ribosomal protein S1/Integration host factor subunit beta
*ACB1221,740,3990TG52.0% 4.2 / 13.7 25T56P (ACC→CCC) CJ019_01643hypothetical protein
*ACB1223,044,6380AC51.8% 6.7 / 19.1 27Q94P (CAG→CCG) rcsC_5Sensor histidine kinase RcsC
*ACB1223,923,4860AC51.8% 1.4 / 19.1 27W423G (TGG→GGG) CJ019_03707hypothetical protein
*ACB122690,1391.G50.0% 7.6 / 11.6 8intergenic (+16/‑2443)bauC/PP_RS08750Putative 3‑oxopropanoate dehydrogenase/tRNA‑Val
*ACB122994,2290TG50.0% 32.6 / 12.3 46D52E (GAT→GAGCJ019_00938hypothetical protein
*ACB1222,094,8080AC50.0% 1.0 / 10.2 37L186R (CTG→CGG) CJ019_01995hypothetical protein
*ACB1225,688,6370AC49.1% 23.2 / 16.9 57S236R (AGC→CGC) pyrC'Dihydroorotase‑like protein
*ACB1223,515,1120TG48.9% 13.9 / 17.6 45intergenic (+36/+365)CJ019_03333/vldHhypothetical protein/Validoxylamine A 7'‑phosphate phosphatase
*ACB1223,626,1060TG47.1% 20.7 / 12.5 34V174G (GTG→GGG) hcf136_3Ycf48‑like protein
*ACB1226,020,3400AC46.7% 26.6 / 18.5 64L58R (CTG→CGG) ycaD_4putative MFS‑type transporter YcaD
*ACB1222,725,6390TG46.5% 5.7 / 13.1 43V640G (GTG→GGG) polBDNA polymerase II
*ACB1223,134,5670TG43.9% 16.8 / 10.8 41L143V (TTG→GTG) CJ019_02952hypothetical protein
*ACB1221,721,8780AC42.9% 11.7 / 14.2 35G95G (GGT→GGGCJ019_01617hypothetical protein

Marginal new junction evidence (sorted from low to high skew)
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? ACB122 = 598141343 (0.520)5 (0.060) 5/238 6.4 10.6% intergenic (+51/‑638) kefC_3/CJ019_05604 Glutathione‑regulated potassium‑efflux systemprotein KefC/hypothetical protein
?ACB122 5981635 = 44 (0.560)intergenic (+273/‑416) kefC_3/CJ019_05604 Glutathione‑regulated potassium‑efflux systemprotein KefC/hypothetical protein
* ? ACB122 2460725 =101 (1.210)3 (0.040) 3/240 7.6 3.0% coding (558/831 nt) queF NADPH‑dependent 7‑cyano‑7‑deazaguaninereductase
?ACB122 3581727 = 101 (1.280)coding (517/1272 nt) ydcO_3 Inner membrane protein YdcO