breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
RA 662,369 T→G K435N (AAA→AAC mrdA ← peptidoglycan DD‑transpeptidase MrdA
RA 874,860 G→A M221I (ATG→ATA yliI → aldose sugar dehydrogenase YliI
RA 924,954 C→T G489R (GGG→AGG)  cydD ← cysteine/glutathione ABC transporter permease/ATP‑binding protein CydD
RA 1,431,900 G→A Q1048* (CAG→TAG)  nifJ ← pyruvate:ferredoxin (flavodoxin) oxidoreductase
RA 1,909,461 G→A Q111* (CAG→TAG)  proQ ← RNA chaperone ProQ
RA 1,986,131 C→A W56C (TGG→TGT pgsA ← CDP‑diacylglycerol‑‑glycerol‑3‑phosphate 3‑phosphatidyltransferase

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NZ_CP009273 1203246 =12 (0.150)62 (0.800) 52/464 1.0 82.5% coding (290/630 nt) BW25113_RS05990 protein StfP
?NZ_CP009273 1205075 = 15 (0.190)coding (40/540 nt) BW25113_RS06005 phage tail protein
* ? NZ_CP009273 = 120326112 (0.150)81 (1.050) 71/464 0.2 86.1% coding (305/630 nt) BW25113_RS05990 protein StfP
?NZ_CP009273 = 1205058 15 (0.190)coding (57/540 nt) BW25113_RS06005 phage tail protein
* ? NZ_CP009273 2282398 =NA (NA)106 (1.280) 88/496 0.0 95.5% pseudogene (3/2532 nt) yejO autotransporter YejO
?NZ_CP009273 = 3801215 5 (0.060)coding (280/1071 nt) rfaQ lipopolysaccharide core heptosyltransferase RfaQ
* ? NZ_CP009273 = 2283592NA (NA)77 (0.930) 65/496 0.5 96.2% intergenic (‑68/‑387) BW25113_RS11435/narP IS5‑like element IS5 family transposase/nitrate/nitrite response regulator protein NarP
?NZ_CP009273 3801212 = 3 (0.040)coding (283/1071 nt) rfaQ lipopolysaccharide core heptosyltransferase RfaQ
* ? NZ_CP009273 2507756 =NA (NA)74 (0.900) 68/492 0.4 94.9% intergenic (+33/‑54) nupC/BW25113_RS12490 nucleoside permease NupC/IS4‑like element IS421 family transposase
?NZ_CP009273 2651919 = 4 (0.050)coding (376/1851 nt) hscA Fe‑S protein assembly chaperone HscA
* ? NZ_CP009273 = 2509092NA (NA)66 (0.810) 62/490 0.6 93.0% intergenic (+170/+30) BW25113_RS12490/pdeA IS4‑like element IS421 family transposase/sensor domain‑containing phosphodiesterase
?NZ_CP009273 = 2651931 5 (0.060)coding (364/1851 nt) hscA Fe‑S protein assembly chaperone HscA
* ? NZ_CP009273 3576788 =NA (NA)88 (1.070) 81/494 0.1 98.9% coding (391/417 nt) BW25113_RS17825 YrhA family protein
?NZ_CP009273 = 3790487 1 (0.010)coding (180/1260 nt) waaL O‑antigen ligase
* ? NZ_CP009273 = 3577554NA (NA)75 (0.910) 66/494 0.5 95.0% intergenic (+14/‑210) BW25113_RS17830/BW25113_RS25760 IS1‑like element IS1A family transposase/protein YrhD
?NZ_CP009273 3790479 = 4 (0.050)coding (172/1260 nt) waaL O‑antigen ligase