breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Marginal read alignment evidence (highest frequency 20 of 325 shown, sorted by frequency from high to low)
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913580,4980GA74.5% 17.9 / 22.6 47V148I (GTT→ATT) nohBbacteriophage DNA packaging protein
*NC_0009133,941,0900TC74.0% 183.1 / 130.3 192noncoding (34/76 nt)gltUtRNA‑Val
*NC_0009131,138,3390GA71.9% 369.0 / 130.4 217V247I (GTT→ATT) flgKhook‑filament junction protein 1
*NC_0009131,931,3390CT71.4% 325.3 / 125.0 196A430A (GCG→GCAedd6‑phosphogluconate dehydratase
*NC_0009131,805,2740TC71.1% 226.2 / 154.5 197L295P (CTG→CCG) pfkB6‑phosphofructokinase II
*NC_00091331,7620GA69.2% 547.4 / 238.5 351G316S (GGT→AGT) carBcarbamoyl‑phosphate synthase large subunit
*NC_0009131,546,3260AG68.9% 215.5 / 175.8 209Y301C (TAC→TGC) fdnGformate dehydrogenase‑N, nitrate‑inducible, alpha subunit
*NC_0009132,460,9001.C68.5% 357.2 / 179.7 165intergenic (+234/‑132)fadL/b2345FadL/hypothetical protein
*NC_0009131,123,3320AG68.4% 166.0 / 143.4 176intergenic (‑55/+9)grxB/yceLglutaredoxin 2/hypothetical protein
*NC_000913880,3160GA68.0% 344.3 / 183.3 247D123N (GAC→AAC) dacCD‑alanyl‑D‑alanine carboxypeptidase
*NC_000913977,6770AG68.0% 199.0 / 177.5 206H710R (CAC→CGC) mukBMukB
*NC_0009132,282,8470TC67.7% 242.3 / 219.9 257V151A (GTG→GCG) yejMputative sulfatase
*NC_0009131,634,0230AT67.6% 33.1 / 35.5 37G123G (GGT→GGAnohANohA
*NC_0009133,489,2640CT66.2% 492.8 / 290.4 370intergenic (‑7/+98)yhfG/ppiAhypothetical protein/peptidyl‑prolyl cis‑trans isomerase A
*NC_0009132,007,5150TC65.4% 160.9 / 204.9 214V5A (GTT→GCT) yedFhypothetical protein
*NC_000913395,8530AG64.1% 217.5 / 304.8 304intergenic (‑342/‑10)yaiH/sbmAhypothetical protein/SbmA
*NC_000913708,0711.G64.1% 452.0 / 292.9 220coding (515/1407 nt)ybfMhypothetical protein
*NC_000913156,8000AG63.8% 197.0 / 256.2 268G28G (GGT→GGCyadNputative fimbrial‑like protein
*NC_0009133,609,4890AG62.5% 183.4 / 307.1 296intergenic (‑83/‑7)yhhS/b3474putative transport/hypothetical protein
*NC_0009133,913,8620TC62.4% 281.5 / inf 474D381G (GAT→GGT) atpDmembrane‑bound ATP synthase, F1 sector, beta‑subunit

Marginal new junction evidence (lowest skew 10 of 16 shown)
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 4099302182 (0.640)92 (0.310)
+GGG
44/164 4.9 33.1% coding (41/993 nt) kdgT 2‑keto‑3‑deoxy‑D‑gluconate transport system
?NC_000913 4099303 = 188 (0.610)coding (42/993 nt) kdgT 2‑keto‑3‑deoxy‑D‑gluconate transport system
* ? NC_000913 = 3423225NA (NA)34 (0.130)
+CCCCCCCCC
33/156 6.1 NA noncoding (1196/2904 nt) rrlD 23S ribosomal RNA
?NC_000913 = 3942515 NA (NA)noncoding (1190/2906 nt) rrlC 23S ribosomal RNA
* ? NC_000913 = 400839899 (0.330)97 (0.330)
+AAGA
27/172 7.8 50.3% coding (481/516 nt) b4555 hypothetical protein
?NC_000913 4008399 = 99 (0.320)coding (482/516 nt) b4555 hypothetical protein
* ? NC_000913 = 284865205 (0.710)67 (0.230)
+GGG
22/166 8.6 24.5% coding (247/1383 nt) yagG putative permease
?NC_000913 284866 = 207 (0.670)coding (248/1383 nt) yagG putative permease
* ? NC_000913 1801057 =203 (0.660)22 (0.070) 13/184 11.5 9.8% intergenic (‑463/‑61) thrS/b1721m threonine tRNA synthetase/ankyrin repeat protein
?NC_000913 2066963 = NA (NA)coding (334/420 nt) b1995 hypothetical protein
* ? NC_000913 1197677 =208 (0.680)6 (0.030) 6/144 12.8 3.2% intergenic (‑217/‑241) ymfE/lit hypothetical protein/Lit
?NC_000913 = 1197708 206 (0.860)intergenic (‑248/‑210) ymfE/lit hypothetical protein/Lit
* ? NC_000913 1197677 =208 (0.680)6 (0.020) 6/160 13.4 2.8% intergenic (‑217/‑241) ymfE/lit hypothetical protein/Lit
?NC_000913 = 1197716 239 (0.900)intergenic (‑256/‑202) ymfE/lit hypothetical protein/Lit
* ? NC_000913 = 3650860NA (NA)7 (0.020) 7/184 13.7 1.5% intergenic (‑32/‑731) trs511/b3506 IS5 transposase/Slp
?NC_000913 3817115 = 466 (1.520)coding (615/618 nt)
coding (1689/1689 nt)
b3646
yicF
hypothetical protein
hypothetical protein
* ? NC_000913 1197677 =208 (0.680)6 (0.020) 6/176 13.9 2.5% intergenic (‑217/‑241) ymfE/lit hypothetical protein/Lit
?NC_000913 = 1197724 264 (0.900)intergenic (‑264/‑194) ymfE/lit hypothetical protein/Lit
* ? NC_000913 = 4475148399 (1.300)6 (0.020) 5/172 14.3 1.6% coding (727/1005 nt) argI ornithine carbamoyltransferase 1
?NC_000913 4475252 = 378 (1.320)coding (623/1005 nt) argI ornithine carbamoyltransferase 1