breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
MC JC 257,908 Δ776 bp insB9[crl] insB9, insA9, [crl]
RA 329,292 T→A I15F (ATC→TTC)  betI ← DNA‑binding transcriptional repressor BetI
MC JC 676,374 Δ11,477 bp IS5‑mediated [ybeQ]gltI [ybeQ], ybeR, djlB, ybeT, ybeU, djlC, hscC, rihA, gltL, gltK, gltJ, sroC, gltI
RA 803,662 C→A L54I (CTT→ATT)  ybhJ → putative hydratase YbhJ
MC JC 1,303,379 Δ11 bp coding (481‑491/921 nt) oppB → murein tripeptide ABC transporter/oligopeptide ABC transporter inner membrane subunit OppB
JC JC 1,743,496 IS5 (–) +4 bp coding (40‑43/1374 nt) mdtK → multidrug efflux pump MdtK
RA 1,905,761 G→A G25D (GGT→GAT)  mntP → Mn(2(+)) exporter
MC JC 1,978,503 Δ776 bp insB5insA5 insB5, insA5
JC JC 1,995,413 IS5 (–) +4 bp intergenic (‑54/‑239) uvrY ← / → yecU DNA‑binding transcriptional activator UvrY/protein YecU
RA 2,173,363 Δ2 bp intergenic (‑490/+918) gatD ← / ← gatB galactitol‑1‑phosphate 5‑dehydrogenase/galactitol‑specific PTS enzyme IIB component
JC JC 2,174,495 IS1 (+) +9 bp coding (64‑72/285 nt) gatB ← galactitol‑specific PTS enzyme IIB component
JC JC 3,262,850 IS5 (–) +4 bp coding (808‑811/1209 nt) tdcD ← propionate kinase
RA 3,560,455 +G intergenic (+589/+167) rtcR → / ← glpG DNA‑binding transcriptional activator RtcR/rhomboid protease GlpG
JC JC 3,939,153 IS2 (+) +5 bp coding (927‑931/993 nt) rbsR → DNA‑binding transcriptional dual regulator RbsR
RA 4,296,381 +GC intergenic (+587/+55) gltP → / ← yjcO glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO
MC JC 4,368,009 Δ802 bp [aspA][fxsA] [aspA], [fxsA]

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 1821689 1821874 186 119 [115] [115] 121 chbB/osmE N,N'‑diacetylchitobiose‑specific PTS enzyme IIB component/osmotically‑inducible lipoprotein OsmE
* * ÷ NC_000913 2839338 2839563 226 119 [117] [117] 119 [ascF] [ascF]
* * ÷ NC_000913 3423716–3424234 3424584–3424238 5–869 122 [116] [116] 120 [rrfD]–[rrlD] [rrfD],[rrlD]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 1207790 =218 (0.520)199 (0.570) 110/160 0.6 46.6% coding (290/630 nt) ycfK protein StfP
?NC_000913 1209619 = 275 (0.780)intergenic (‑500/‑66) tfaE/pinE putative tail fiber assembly protein TfaE/site‑specific DNA recombinase
* ? NC_000913 = 1207805218 (0.520)149 (0.430) 81/160 2.0 39.5% coding (305/630 nt) ycfK protein StfP
?NC_000913 = 1209602 275 (0.780)intergenic (‑483/‑83) tfaE/pinE putative tail fiber assembly protein TfaE/site‑specific DNA recombinase
* ? NC_000913 = 12994980 (0.000)544 (1.350) 166/184 0.0 99.9% intergenic (+253/‑69) ychE/insH21 MarC family putative inner membrane protein YchE/IS5 family transposase and trans‑activator
?NC_000913 1300698 = 1 (0.000)intergenic (+151/‑484) insH21/oppA IS5 family transposase and trans‑activator/oligopeptide ABC transporter periplasmic binding protein