Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC JC	REL606	16,990	IS150 (+) +3 bp	intergenic (‑32/‑496)	mokC ← / → nhaA	regulatory protein for HokC, overlaps CDS of hokC/pH‑dependent sodium/proton antiporter

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	REL606	16990 =	0 (0.000)	48 (1.050)	35/150	0.3	100%	intergenic (‑32/‑498)	mokC/nhaA	regulatory protein for HokC, overlaps CDS of hokC/pH‑dependent sodium/proton antiporter
	?	REL606	= 590471	NA (NA)					noncoding (1977/1977 nt)	IS150	repeat region
*	?	REL606	= 16992	0 (0.000)	63 (1.380)	41/150	0.1	100%	intergenic (‑34/‑496)	mokC/nhaA	regulatory protein for HokC, overlaps CDS of hokC/pH‑dependent sodium/proton antiporter
	?	REL606	= 2775877	NA (NA)					noncoding (1/1443 nt)	IS150	repeat region

TATAATAGCGGCGGGTGCTTGAGGCTATCTGTCTCAGGCATTAGCTGAACGGCAGATAGAGAAAAGCCCCGAGT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  REL606/17063‑16990 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑TGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTCTGATTCCGATATTCAATTGGAGTCAGA  <  REL606/590471‑590397                                                                                                                                                       TATAATAGCGGCGGGTGCTTGAGGCTATCTGTCTCAGGCATTAGCTGAACGGCAGATAGAGAAAAGCCCCGAGTTG                                                                           <  1:922544/76‑1  ATAATAGCGGCGGGGGCTTGAGGCTATCTGTCTCAGGCATTAGCTGAACGGCAGATAGAGAAAAGCCCCGAGTTGT                                                                          >  2:1406643/1‑76      TAGCGGCGGGTGCTTGAGGCTATCTGTCTCAGGCATTAGCTGAACGGCAGATAGAGAAAAGCCCCGAGTTGTACTG                                                                      <  2:365575/76‑1      TAGCGGCGGGTGCTTGAGGCTATCTGTCTCAGGCATTAGCTGAACGGCAGATAGAGAAAAGCCCCGAGTTGTACTG                                                                      <  2:279049/76‑1            CGGGTGCTTGAGGCTATCTGTCTCAGGCATTAGCTGAACGGCAGATAGAGAAAAGCCCCGAGTTGTACTGACCCCA                                                                <  2:1413374/76‑1               GTGCTTGAGGCTATCTGTCTCAGGCATTAGCTGAACGGCAGATAGAGAAAATCCCCGAGTTNTACTGACCCCAAAA                                                             <  1:1581/76‑1                      AGGCTATCTGTCTCAGGCATTAGCTGAACGGCAGATAGAGAAAAGCCCCGAGTTGTACTGACCCCAAAAAGTTGGA                                                      >  2:1357882/1‑76                       GGCTATCTGTCTCATGCATTAGCTGAACGGTAGATAGAGTAAAGCCCCGAGTTGTACTGACCCCAAAAAGTTGGAC                                                     <  1:31938/76‑1                       GGCTATCTGTCTCAGGCATNAGCTGAACGGCAGATAGAGAAAAGCCCCGAGTTGTACTGACCCCAAAAAGTTGGAC                                                     <  1:790211/76‑1                         CTATCTTTCTNACTCATTAGCNGAACGGCAGATAGAGAAAAGCCCCGAGTTGTACTGACCCCAAAAAGTTGGACAG                                                   >  2:1462126/1‑76                         CTATCTGTCTCAGGCATTAGCTGAACGGCAGATAGAGAAAAGCCCCGAGTTGTACTGACCCCAAAAAGTTGGACAG                                                   >  1:114398/1‑76                          TATCTGTCTCAGGCATTAGCTGAACGGCAGATAGAGAAAAGCCCCGAGTTGTACTGACCCCAAAAAGTTGGACAGT                                                  >  1:1135764/1‑76                          TATCTGTCTCAGGCATTAGCTGAACGGCAGATAGAGAAAAGCCCCGAGTTGTACTGACCCCAAAAAGTTGGACAGT                                                  >  2:624030/1‑76                            TCTGTCTCAGGCATTAGCTGAACGGCAGATAGAGAAAAGCCCCGAGTTGTACTGACCCCAAAAAGTTGGACAGTTA                                                <  2:933471/76‑1                             CTGTCTCAGGCATTAGCTGAACGGCAGATAGAGAAAAGCCCCGAGTTGTACTGACCCCAAAAAGTTGGACAGTTAA                                               <  2:1402213/76‑1                               GTCTCAGGCATTAGCTGAACGGCAGATAGAGAAAAGCCCCGAGTTGTACTGACCCCAAAAAGTTGGACAGTTAAAC                                             <  2:169074/76‑1                                 CTCAGGCATTAGCTGAACGGCAGATAGAGAAAAGCCCCGAGTTGTACTGACCCCAAAAAGTTGGACAGTTAAACAC                                           >  1:809058/1‑76                                        ATTAGCTGAACGGCAGATAGAGAAAAGCCCCGAGTTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCAT                                    <  1:139399/76‑1                                           AGCTGAACGGCAGATAGAGAAAAGCCCCGAGTTGTACTGACCCTAAAAAGTTGGACAGTTAAACACGAGGCATATA                                 >  1:298293/1‑76                                           AGCTGAACGGCAGATAGAGAAAAGCCCCGAGTTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATA                                 <  2:702783/76‑1                                             CTGAACGGCAGATAGAGAAAAGCCCCGAGTTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGG                               <  1:348723/76‑1                                               GAACGGCAGATAGAGAAAAGCCCCGAGTTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTC                             >  2:1164157/1‑76                                                  CGGCAGATAGAGAAAAGCCCCGAGTTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTCTGA                          >  2:914938/1‑76                                                   GGCAGATAGAGAAAAGCCCCGAGTTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTCTGAT                         <  2:738754/76‑1                                                    GCAGATAGAGAAAAGCCCCGAGTTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTCTGATT                        <  1:388824/76‑1                                                    GCAGATAGAGAAAAGCCCCGAGTTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTCTGATT                        <  2:1058604/76‑1                                                     CAGATAGAGAAAAGCCCCGAGTTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTCTGATTC                       >  2:848263/1‑76                                                         TAGAGAAAAGCCCCGAGTTGTACTGACCCCAGAANGTTGGACNGTNAANCACGAGGCGTATAGGTCTGATTCCGAT                   >  1:1278728/1‑76                                                         TAGAGAAAAGCCCCGAGTTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTCTGATTCCGAT                   <  1:1023687/76‑1                                                         TAGAGAAAAGCCCCGAGTTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTCTGATTCCGAT                   <  1:496400/76‑1                                                          AGAGAAAAGCCCCGAGTTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTCTGATTCCGATA                  >  2:313404/1‑76                                                            AGAAAAGCCCCGAGTTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTCTGATTCCGATATT                <  1:285242/76‑1                                                             GAAAAGCCCCGAGTTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTCTGATTCCGATATTC               >  1:853646/1‑76                                                             GAAAAGCCCCGAGTTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTCTGATTCCGATATTC               <  2:733285/76‑1                                                              AAAAGCCCCGAGTTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTCTGATTCCGATATTCA              <  1:370228/76‑1                                                              AAAAGCCCCGAGTTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTCTGATTCCGATATTCA              >  2:743875/1‑76                                                               AAAGCCCCGAGTTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTCTGATTCCGATATTCAA             >  2:822469/1‑76                                                                     CCGAGTTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTCTGATTCCGATATTCAATTGGAG       <  2:1161664/76‑1                                                                       tacTTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTCTGATTCCGATATTCAATTGGAGTC     >  1:786999/4‑76                                                                       GAGTTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTCTGATTCCGATATTCAATTGGAGTC     >  2:383555/1‑76                                                                       GAGTTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTCTGATTCCGATATTCAATTGGAGTC     <  2:1285264/76‑1                                                                       GAGTTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTCTGATTCCGATATTCAATTGGAGTC     <  1:911809/76‑1                                                                        AGTTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTCTGATTCCGATATTCAATTGGAGTCA    >  2:569475/1‑76                                                                         GTTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTCTGATTCCGATATTCAATTGGAGTCAG   <  2:679016/76‑1                                                                         GTTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTCTGATTCCGATATTCAATTGGAGTCAG   <  2:789284/76‑1                                                                          TTGTACTGACCNCANAAAGTTGGACAGTTAAACACGAGGCATATAGGTCTGATTCCGTCATTCAATCGGAGTCAGA  <  2:227926/76‑1                                                                          TTGTACTGACCCCAAAAAGTTGGGCAGTTAAACACGAGGCATATAGGTCTGATTCCGATATTCAATTGGAGTCAGA  >  1:320108/1‑76                                                                          TTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTCTGATTCCGATATTCAATTGGAGTCAGA  <  2:910522/76‑1                                                                                                                                                       TATAATAGCGGCGGGTGCTTGAGGCTATCTGTCTCAGGCATTAGCTGAACGGCAGATAGAGAAAAGCCCCGAGT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  REL606/17063‑16990 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑TGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTCTGATTCCGATATTCAATTGGAGTCAGA  <  REL606/590471‑590397

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.