Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
MC JC	REL606	1,290,853	Δ92 bp	coding (818‑909/1014 nt)	hnr →	response regulator of RpoS

Missing coverage evidence...
			seq id	start	end	size	←reads	reads→	gene	description
*	*	÷	REL606	1290853	1290944	92	62 [0]	[0] 62	hnr	response regulator of RpoS

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	REL606	= 1290852	0 (0.000)	62 (1.290)	44/148	0.1	100%	coding (817/1014 nt)	hnr	response regulator of RpoS
	?	REL606	1290945 =	0 (0.000)					coding (910/1014 nt)	hnr	response regulator of RpoS

AAACCATTTACTCCGTCAGGCCAATCTGCCGGGGCAGTTTCCGCTATTAGTTGGCTATTATCATCGCGAACTGAAAAATCTCATTCTGGTTTCTGCGGGTCTGAATGCGACGTTAAATACCGGCGAACACCAGGTGCAAATCAGTAAT  >  REL606/1290779‑1290926                                                                          |                                                                           aaaCCATTTACTCCGTCAGGCCAATCTGCCGGGGCAGTTTCCGCTATTAGTTGGCTATTATCATCGCGAACTGAtt                                                                          >  2:1275107‑M1/1‑74 (MQ=255)  aaCCATTTACTCCGTCAGGCCAATCTGCCGGGGCAGTTTCCGCTATTAGTTGGCTATTATCATCGCGAACTGAtta                                                                         >  2:7136‑M1/1‑73 (MQ=255)   aCCATTTACTCCGTCAGGCCAATCTGCCGGGGCAGTTTCCGCTATTAGTTGGCTATTATCATCGCGAACTGAttag                                                                        <  2:421707‑M1/76‑5 (MQ=255)     cATTTACTCCGTCAGGCCAATCTGCCGGGGCAGTTTCCGCTATTAGTTGGCTATTATCATCGCGAACTGAttaggt                                                                      >  1:1369870‑M1/1‑70 (MQ=255)      aTTTACTCCGTCAGGCCAATCTGCCGGGGCAGTTTCCGCTATTAGTTGGCTATTATCATCGCGAACTGAttaggta                                                                     >  1:1002778‑M1/1‑69 (MQ=255)      aTTTACTCCGTCAGGCCAATCTGCCGGGGCAGTTTCCGCTATTAGTTGGCTATTATCATCGCGAACTGAttaggta                                                                     >  1:1108588‑M1/1‑69 (MQ=255)      aTTTACTCCGTCAGGCCAATCTGCCGGGGCAGTTTCCGCTATTAGTTGGCTATTATCATCGCGAACTGAttaggta                                                                     >  1:1108580‑M1/1‑69 (MQ=255)       tttACTCCGTCAGGCCAATCTGCCGGGGCAGTTTCCGCTATTAGTTGGCTATTATCATCGCGAACTGAttaggtaa                                                                    <  1:1432462‑M1/76‑9 (MQ=255)        ttACTCCGTCAGGCCAATCTGCCGGGGCAGTTTCCGCTATTAGTTGGCTATTATCATCGCGAACTGAttaggtaac                                                                   >  1:592331‑M1/1‑67 (MQ=255)         tACTCCGTCAGGCCAATCTGCCGGGGCAGTTTCCGCTATTAGTTGGCTATTATCATCGCGAACTGAttaggtaacg                                                                  >  1:702753‑M1/1‑66 (MQ=255)         tACTCCGTCAGGCCAATCTGCCGGGGCAGTTTCCGCTATTAGTTGGCTATTATCATCGCGAACTGAttaggtaacg                                                                  <  1:1165341‑M1/76‑11 (MQ=255)         tACTCCGTCAGGCCAATCTGCCGGGGCAGTTTCCGCTATTAGTTGGCTATTATCATCGCGAACTGAttaggtaacg                                                                  <  1:986‑M1/76‑11 (MQ=255)         tACTCCGTCAGGCCAATCTGCCGGGGCAGTTTCCGCTATTAGTTGGCTATTATCATCGCGAACTGAttaggtaacg                                                                  >  1:424785‑M1/1‑66 (MQ=255)         tACTCCGTCAGGCCAATCTGCCGGGGCAGTTTCCGCTATTAGTTGGCTATTATCATCGCGAACTGAttaggtaacg                                                                  >  1:424799‑M1/1‑66 (MQ=255)             ccGTCAGGCCAATCTGCCGGGGCAGTTTCCGCTATTAGTTGGCTATTATCATCGCGAACTGAttaggtaacgctta                                                              >  2:861338‑M1/1‑62 (MQ=255)             ccGTCAGGCCAATCTGCCGGGGCAGTTTCCGCTATTAGTTGGCTATTATCATCGCGAACTGAttaggtaacgctta                                                              <  1:1025614‑M1/76‑15 (MQ=255)                tccggccaATCTGCCGGGGCAGTTTCCGCTATTAGTTGGCTATTATCATCGCGAACTGAttaggtaacgcttattt                                                           <  2:694537‑M1/73‑18 (MQ=255)                tCAGGCCAATCTGCCGGGGCAGTTTCCGCTATTAGTTGGCTATTATCATCGCGAACTGAttaggtaacgcttattt                                                           >  2:1028739‑M1/1‑59 (MQ=255)                tCAGGCCAATCTGCCGGGGCAGTTTCCGCTATTAGTTGGCTATTATCATCGCGAACTGAttaggtaacgcttattt                                                           >  2:510399‑M1/1‑59 (MQ=255)                tCAGGCCAATCTGCCGGGGCAGTTTCCGCTATTAGTTGGCTATTATCATCGCGAACTGAttaggtaacgcttattt                                                           <  2:1117777‑M1/76‑18 (MQ=255)                tCAGGCCAATCTGCCGGGGCAGTTTCCGCTATTAGTTGGCTATTATCATCGCGAACTGAttaggtaacgcttattt                                                           >  2:754821‑M1/1‑59 (MQ=255)                  aGGCCAATCTGCCGGGGCAGTTTCCGCTATTAGTTGGCTATTATCATCGCGAACTGAttaggtaacgcttatttga                                                         <  2:1015953‑M1/76‑20 (MQ=255)                    gCCAATCTGCCGGGGCAGTTTCCGCTATTAGTTGGCTATTATCATCGCGAACTGAttaggtaacgcttatttgaat                                                       <  2:331894‑M1/76‑22 (MQ=255)                      cAATCTGCCGGGGCAGTTTCCGCTATTAGTTGGCTATTATCATCGCGAACTGAttaggtaacgcttatttgaatca                                                     <  2:1018641‑M1/76‑24 (MQ=255)                      cAATCTGCCGGGGCAGTTTCCGCTATTAGTTGGCTATTATCATCGCGAACTGAttaggtaacgcttatttgaatca                                                     >  1:919963‑M1/1‑53 (MQ=255)                       aaTCTGCCGGGGCAGTTTCCGCTATTAGTTGGCTATTATCATCGCGAACTGAttaggtaacgcttatttgaatcaa                                                    <  1:367286‑M1/76‑25 (MQ=255)                          cTGCCGGGGCAGTTTCCGCTATTAGTTGGCTATTATCATCGCGAACTGAttaggtaacgcttatttgaatcaattg                                                 >  1:981243‑M1/1‑49 (MQ=255)                                gggCAGTTTCCGCTATTAGTTGGCTATTATCATCGCGAACTGAttaggtaacgcttatttgaatcaattgagccag                                           >  1:840151‑M1/1‑43 (MQ=255)                                gggCAGTTTCCGCTATTAGTTGGCTATTATCATCGCGAACTGAttaggtaacgcttatttgaatcaattgagccag                                           <  1:95938‑M1/76‑34 (MQ=255)                                  gCAGTTTCCGCTATTAGTTGGCTATTATCATCGCGAACTGAttaggtaacgcttatttgaatcaattgagccagcg                                         >  1:609696‑M1/1‑41 (MQ=255)                                    aGTTTCCGCTATTAGTTGGCTATTATCATCGCGAACTGAttaggtaacgcttatttgaatcaattgagccagcgat                                       >  1:1161477‑M1/1‑39 (MQ=255)                                     gTTTCCGCTATTAGTTGGCTATTATCATCGCGAACTGAttaggtaacgcttatttgaatcaattgagccagcgatg                                      <  2:919963‑M1/76‑39 (MQ=255)                                      tttCCGCTATTAGTTGGCTATTATCATCGCGAACTGAttaggtaacgcttatttgaatcaattgagccagcgatgc                                     >  2:184436‑M1/1‑37 (MQ=255)                                        tCCGCTATTAGTTGGCTATTATCATCGCGAACTGAttaggtaacgcttatttgaatcaattgagccagcgatgcga                                   >  1:507767‑M1/1‑35 (MQ=255)                                         ccGCTATTAGTTGGCTATTATCATCGCGAACTGAttaggtaacgcttatttgaatcaattgagccagcgatgcgat                                  <  2:1020676‑M1/76‑43 (MQ=255)                                           gCTATTAGTTGGCTATTATCATCGCGAACTGAttaggtaacgcttatttgaatcaattgagccagcgatgcgatgc                                >  1:1134866‑M1/1‑32 (MQ=255)                                            cTATTAGTTGGCTATTATCATCGCGAACTGAttaggtaacgcttatttgaatcaattgagccagcgatgcgatgcc                               >  2:1401872‑M1/1‑31 (MQ=255)                                              aTTAGTTGGCTATTATCATCGCGAACTGAttaggtaacgcttatttgaatcaattgagccagcgatgcgatgcctg                             <  2:84099‑M1/76‑48 (MQ=255)                                              aTTAGTTGGCTATTATCATCGCGAACTGAttaggtaacgcttatttgaatcaattgagccagcgatgcgatgcctg                             <  2:1074239‑M1/76‑48 (MQ=255)                                              aTTAGTTGGCTATTATCATCGCGAACTGAttaggtaacgcttatttgaatcaattgagccagcgatgcgatgcctg                             >  2:957407‑M1/1‑29 (MQ=255)                                              aTTAGTTGGCTATTATCATCGCGAACTGAttaggtaacgcttatttgaatcaattgagccagcgatgcgatgcctg                             <  1:470208‑M1/76‑48 (MQ=255)                                                  gTTGGCTATTATCATCGCGAACTGAttaggtaacgcttatttgaatcaattgagccagcgatgcgatgcctggcaa                         >  1:598539‑M1/1‑25 (MQ=255)                                                    tGGCTATTATCATCGCGAACTGAttaggtaacgcttatttgaatcaattgagccagcgatgcgatgcctggcaatg                       >  1:795679‑M1/1‑23 (MQ=255)                                                       cTATTATCATCGCGAACTGAttaggtaacgcttatttgaatcaattgagccagcgatgcgatgcctggcaatgcca                    >  2:261363‑M1/1‑20 (MQ=255)                                                        tattatCATCGCGAACTGAttaggtaacgcttatttgaatcaattgagccagcgatgcgatgcctggcaatgccaa                   >  2:125293‑M1/1‑19 (MQ=255)                                                        tattatCATCGCGAACTGAttaggtaacgcttatttgaatcaattgagccagcgatgcgatgcctggcaatgccaa                   >  2:746525‑M1/1‑19 (MQ=255)                                                          ttatCATCGCGAACTGAttaggtaacgcttatttgaatcaattgagccagcgatgcgatgcctggcaatgccaaat                 <  1:467288‑M1/76‑60 (MQ=255)                                                            atcatcGCGAACTGAttaggtaacgcttatttgaatcaattgagccagcgatgcgatgcctggcaatgccaaatat               <  1:1002726‑M1/76‑62 (MQ=255)                                                                  gcgAACTGAttaggtaacgcttatttgaatcaattgagccagcgatgcgatgcctggcaatgccaaatatggggaa         <  2:1210370‑M1/76‑68 (MQ=255)                                                                   cgAACTGAttaggtaacgcttatttgaatcaattgagccagcgatgcgatgcctggcaatgccaaatatggggaac        >  2:350986‑M1/1‑8 (MQ=255)                                                                    gAACTGAttaggtaacgcttatttgaatcaattgagccagcgatgcgatgcctggcaatgccaaatatggggaacc       >  2:537613‑M1/1‑7 (MQ=255)                                                                       cTGAttaggtaacgcttatttgaatcaattgagccagcgatgcgatgcctggcaatgccaaatatggggaaccggt    >  1:556429‑M1/1‑4 (MQ=255)                                                                       cTGAttaggtaacgcttatttgaatcaattgagccagcgatgcgatgcctggcaatgccaaatatggggaaccggt    >  1:1472775‑M1/1‑4 (MQ=255)                                                                        tGAttaggtaacgcttatttgaatcaattgagccagcgatgcgatgcctggcaatgccaaatatggggaaccggtg   >  1:597520‑M1/1‑3 (MQ=255)                                                                        tGAttaggtaacgcttatttgaatcaattgagccagcgatgcgatgcctggcaatgccaaatatggggaaccggtg   >  1:710988‑M1/1‑3 (MQ=255)                                                                        tGAttaggtaacgcttatttgaatcaattgagccagcgatgcgatgcctggcaatgccaaatatggggaaccggtg   >  1:353573‑M1/1‑3 (MQ=255)                                                                        tGAttaggtaacgcttatttgaatcaattgagccagcgatgcgatgcctggcaatgccaaatatggggaaccggtg   <  2:667872‑M1/76‑74 (MQ=255)                                                                        tGAttaggtaacgcttatttgaatcaattgagccagcgatgcgatgcctggcaatgccaaatatggggaaccggtg   >  1:389081‑M1/1‑3 (MQ=255)                                                                        tGAttaggtaacgcttatttgaatcaattgagccagcgatgcgatgcctggcaatgccaaatatggggaaccggag   >  1:41812‑M1/1‑3 (MQ=255)                                                                         gAttaggtaacgcttatttgaatcaattgagccagcgatgcgatgcctggcaatgccaaatatggggaaccggtgg  >  2:829098‑M1/1‑2 (MQ=255)                                                                         gAttaggtaacgcttatttgaatcaattgagccagcgatgcgatgcctggcaatgccaaatatggggaaccggtgg  >  2:1198924‑M1/1‑2 (MQ=255)                                                                         gAttaggtaacgcttatttgaatcaattgagccagcgatgcgatgcctggcaatgccaaatatggggaaccggctc  >  2:960746‑M1/1‑2 (MQ=255)                                                                          |                                                                           AAACCATTTACTCCGTCAGGCCAATCTGCCGGGGCAGTTTCCGCTATTAGTTGGCTATTATCATCGCGAACTGAAAAATCTCATTCTGGTTTCTGCGGGTCTGAATGCGACGTTAAATACCGGCGAACACCAGGTGCAAATCAGTAAT  >  REL606/1290779‑1290926

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑