Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
MC JC	REL606	1,290,853	Δ92 bp	coding (818‑909/1014 nt)	hnr →	response regulator of RpoS

Missing coverage evidence...
			seq id	start	end	size	←reads	reads→	gene	description
*	*	÷	REL606	1290853	1290944	92	62 [0]	[0] 62	hnr	response regulator of RpoS

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	REL606	= 1290852	0 (0.000)	62 (1.290)	44/148	0.1	100%	coding (817/1014 nt)	hnr	response regulator of RpoS
	?	REL606	1290945 =	0 (0.000)					coding (910/1014 nt)	hnr	response regulator of RpoS

CTGCGGGTCTGAATGCGACGTTAAATACCGGCGAACACCAGGTGCAAATCAGTAATGGTGTTCCGTTAGGCACATTAGGTAACGCTTATTTGAATCAATTGAGCCAGCGATGCGATGCCTGGCAATGCCAAATATGGGGAACCGGTGG  >  REL606/1290871‑1291018                                                                           |                                                                          aaaccatttactccgtcaggccaatctgccggggcagtttccgctattagttggctattatcatcgcgaactgatt                                                                          >  2:1275107‑M2/75‑76 (MQ=255)  aaccatttactccgtcaggccaatctgccggggcagtttccgctattagttggctattatcatcgcgaactgaTTa                                                                         >  2:7136‑M2/74‑76 (MQ=255)   accatttactccgtcaggccaatctgccggggcagtttccgctattagttggctattatcatcgcgaactgaTTAg                                                                        <  2:421707‑M2/4‑1 (MQ=255)     catttactccgtcaggccaatctgccggggcagtttccgctattagttggctattatcatcgcgaactgaTTAGGt                                                                      >  1:1369870‑M2/71‑76 (MQ=255)      atttactccgtcaggccaatctgccggggcagtttccgctattagttggctattatcatcgcgaactgaTTAGGTa                                                                     >  1:1002778‑M2/70‑76 (MQ=255)      atttactccgtcaggccaatctgccggggcagtttccgctattagttggctattatcatcgcgaactgaTTAGGTa                                                                     >  1:1108588‑M2/70‑76 (MQ=255)      atttactccgtcaggccaatctgccggggcagtttccgctattagttggctattatcatcgcgaactgaTTAGGTa                                                                     >  1:1108580‑M2/70‑76 (MQ=255)       tttactccgtcaggccaatctgccggggcagtttccgctattagttggctattatcatcgcgaactgaTTAGGTaa                                                                    <  1:1432462‑M2/8‑1 (MQ=255)        ttactccgtcaggccaatctgccggggcagtttccgctattagttggctattatcatcgcgaactgaTTAGGTAAc                                                                   >  1:592331‑M2/68‑76 (MQ=255)         tactccgtcaggccaatctgccggggcagtttccgctattagttggctattatcatcgcgaactgaTTAGGTAACg                                                                  >  1:702753‑M2/67‑76 (MQ=255)         tactccgtcaggccaatctgccggggcagtttccgctattagttggctattatcatcgcgaactgaTTAGGTAACg                                                                  <  1:1165341‑M2/10‑1 (MQ=255)         tactccgtcaggccaatctgccggggcagtttccgctattagttggctattatcatcgcgaactgaTTAGGTAACg                                                                  <  1:986‑M2/10‑1 (MQ=255)         tactccgtcaggccaatctgccggggcagtttccgctattagttggctattatcatcgcgaactgaTTAGGTAACg                                                                  >  1:424785‑M2/67‑76 (MQ=255)         tactccgtcaggccaatctgccggggcagtttccgctattagttggctattatcatcgcgaactgaTTAGGTAACg                                                                  >  1:424799‑M2/67‑76 (MQ=255)             ccgtcaggccaatctgccggggcagtttccgctattagttggctattatcatcgcgaactgaTTAGGTAACGCTTa                                                              >  2:861338‑M2/63‑76 (MQ=255)             ccgtcaggccaatctgccggggcagtttccgctattagttggctattatcatcgcgaactgaTTAGGTAACGCTTa                                                              <  1:1025614‑M2/14‑1 (MQ=255)                tccggccaatctgccggggcagtttccgctattagttggctattatcatcgcgaactgaTTAGGTAACGCTTAttt                                                           <  2:694537‑M2/17‑1 (MQ=255)                tcaggccaatctgccggggcagtttccgctattagttggctattatcatcgcgaactgaTTAGGTAACGCTTAttt                                                           >  2:1028739‑M2/60‑76 (MQ=255)                tcaggccaatctgccggggcagtttccgctattagttggctattatcatcgcgaactgaTTAGGTAACGCTTAttt                                                           >  2:510399‑M2/60‑76 (MQ=255)                tcaggccaatctgccggggcagtttccgctattagttggctattatcatcgcgaactgaTTAGGTAACGCTTAttt                                                           <  2:1117777‑M2/17‑1 (MQ=255)                tcaggccaatctgccggggcagtttccgctattagttggctattatcatcgcgaactgaTTAGGTAACGCTTAttt                                                           >  2:754821‑M2/60‑76 (MQ=255)                  aggccaatctgccggggcagtttccgctattagttggctattatcatcgcgaactgaTTAGGTAACGCTTATTTGa                                                         <  2:1015953‑M2/19‑1 (MQ=255)                    gccaatctgccggggcagtttccgctattagttggctattatcatcgcgaactgaTTAGGTAACGCTTATTTGAAt                                                       <  2:331894‑M2/21‑1 (MQ=255)                      caatctgccggggcagtttccgctattagttggctattatcatcgcgaactgaTTAGGTAACGCTTATTTGAATCa                                                     <  2:1018641‑M2/23‑1 (MQ=255)                      caatctgccggggcagtttccgctattagttggctattatcatcgcgaactgaTTAGGTAACGCTTATTTGAATCa                                                     >  1:919963‑M2/54‑76 (MQ=255)                       aatctgccggggcagtttccgctattagttggctattatcatcgcgaactgaTTAGGTAACGCTTATTTGAATCaa                                                    <  1:367286‑M2/24‑1 (MQ=255)                          ctgccggggcagtttccgctattagttggctattatcatcgcgaactgaTTAGGTAACGCTTATTTGAATCAATTg                                                 >  1:981243‑M2/50‑76 (MQ=255)                                gggcagtttccgctattagttggctattatcatcgcgaactgaTTAGGTAACGCTTATTTGAATCAATTGAGCCAg                                           >  1:840151‑M2/44‑76 (MQ=255)                                gggcagtttccgctattagttggctattatcatcgcgaactgaTTAGGTAACGCTTATTTGAATCAATTGAGCCAg                                           <  1:95938‑M2/33‑1 (MQ=255)                                  gcagtttccgctattagttggctattatcatcgcgaactgaTTAGGTAACGCTTATTTGAATCAATTGAGCCAgcg                                         >  1:609696‑M2/42‑76 (MQ=255)                                    agtttccgctattagttggctattatcatcgcgaactgaTTAGGTAACGCTTATTTGAATCAATTGAGCCAgcgat                                       >  1:1161477‑M2/40‑76 (MQ=255)                                     gtttccgctattagttggctattatcatcgcgaactgaTTAGGTAACGCTTATTTGAATCAATTGAGCCAgcgatg                                      <  2:919963‑M2/38‑1 (MQ=255)                                      tttccgctattagttggctattatcatcgcgaactgaTTAGGTAACGCTTATTTGAATCAATTGAGCCAgcgatgc                                     >  2:184436‑M2/38‑76 (MQ=255)                                        tccgctattagttggctattatcatcgcgaactgaTTAGGTAACGCTTATTTGAATCAATTGAGCCAgcgatgcga                                   >  1:507767‑M2/36‑76 (MQ=255)                                         ccgctattagttggctattatcatcgcgaactgaTTAGGTAACGCTTATTTGAATCAATTGAGCCAgcgatgcgat                                  <  2:1020676‑M2/42‑1 (MQ=255)                                           gctattagttggctattatcatcgcgaactgaTTAGGTAACGCTTATTTGAATCAATTGAGCCAGCgatgcgatgc                                >  1:1134866‑M2/33‑76 (MQ=255)                                            ctattagttggctattatcatcgcgaactgaTTAGGTAACGCTTATTTGAATCAATTGAGCCAGCGATGCGATGcc                               >  2:1401872‑M2/32‑76 (MQ=255)                                              attagttggctattatcatcgcgaactgaTTAGGTAACGCTTATTTGAATCAATTGAGCCAGCGATGCGATGCCTg                             <  2:84099‑M2/47‑1 (MQ=255)                                              attagttggctattatcatcgcgaactgaTTAGGTAACGCTTATTTGAATCAATTGAGCCAGCGATGCGATGCCTg                             <  2:1074239‑M2/47‑1 (MQ=255)                                              attagttggctattatcatcgcgaactgaTTAGGTAACGCTTATTTGAATCAATTGAGCCAGCGATGCGATGCCTg                             >  2:957407‑M2/30‑76 (MQ=255)                                              attagttggctattatcatcgcgaactgaTTAGGTAACGCTTATTTGAATCAATTGAGCCAGCGATGCGATGCCTg                             <  1:470208‑M2/47‑1 (MQ=255)                                                  gttggctattatcatcgcgaactgaTTAGGTAACGCTTATTTGAATCAATTGAGCCAGCGATGCGATGCCTGGCaa                         >  1:598539‑M2/26‑76 (MQ=255)                                                    tggctattatcatcgcgaactgaTTAGGTAACGCTTATTTGAATCAATTGAGCCAGCGATGCGATGCCTGGCAATg                       >  1:795679‑M2/24‑76 (MQ=255)                                                       ctattatcatcgcgaactgaTTAGGTAACGCTTATTTGAATCAATTGAGCCAGCGATGCGATGCCTGGCAATGCCa                    >  2:261363‑M2/21‑76 (MQ=255)                                                        tattatcatcgcgaactgaTTAGGTAACGCTTATTTGAATCAATTGAGCCAGCGATGCGATGCCTGGCAATGCCaa                   >  2:125293‑M2/20‑76 (MQ=255)                                                        tattatcatcgcgaactgaTTAGGTAACGCTTATTTGAATCAATTGAGCCAGCGATGCGATGCCTGGCAATGCCaa                   >  2:746525‑M2/20‑76 (MQ=255)                                                          ttatcatcgcgaactgaTTAGGTAACGCTTATTTGAATCAATTGAGCCAGCGATGCGATGCCTGGCAATGCCAAat                 <  1:467288‑M2/59‑1 (MQ=255)                                                            atcatcgcgaactgaTTAGGTAACGCTTATTTGAATCAATTGAGCCAGCGATGCGATGCCTGGCAATGCCAAatat               <  1:1002726‑M2/61‑1 (MQ=255)                                                                  gcgaactgaTTAGGTAACGCTTATTTGAATCAATTGAGCCAGCGATGCGATGCCTGGCAATGCCAAATATGGGGaa         <  2:1210370‑M2/67‑1 (MQ=255)                                                                   cgaactgaTTAGGTAACGCTTATTTGAATCAATTGAGCCAGCGATGCGATGCCTGGCAATGCCAAATATGGGGAAc        >  2:350986‑M2/9‑76 (MQ=255)                                                                    gaactgaTTAGGTAACGCTTATTTGAATCAATTGAGCCAGCGATGCGATGCCTGGCAATGCCAAATATGGGGAAcc       >  2:537613‑M2/8‑76 (MQ=255)                                                                       ctgaTTAGGTAACGCTTATTTGAATCAATTGAGCCAGCGATGCGATGCCTGGCAATGCCAAATATGGGGAACCggt    >  1:556429‑M2/5‑76 (MQ=255)                                                                       ctgaTTAGGTAACGCTTATTTGAATCAATTGAGCCAGCGATGCGATGCCTGGCAATGCCAAATATGGGGAACCggt    >  1:1472775‑M2/5‑76 (MQ=255)                                                                        tgaTTAGGTAACGCTTATTTGAATCAATTGAGCCAGCGATGCGATGCCTGGCAATGCCAAATATGGGGAAccggag   >  1:41812‑M2/4‑74 (MQ=255)                                                                        tgaTTAGGTAACGCTTATTTGAATCAATTGAGCCAGCGATGCGATGCCTGGCAATGCCAAATATGGGGAACCggtg   >  1:597520‑M2/4‑76 (MQ=255)                                                                        tgaTTAGGTAACGCTTATTTGAATCAATTGAGCCAGCGATGCGATGCCTGGCAATGCCAAATATGGGGAACCggtg   >  1:710988‑M2/4‑76 (MQ=255)                                                                        tgaTTAGGTAACGCTTATTTGAATCAATTGAGCCAGCGATGCGATGCCTGGCAATGCCAAATATGGGGAACCggtg   >  1:353573‑M2/4‑76 (MQ=255)                                                                        tgaTTAGGTAACGCTTATTTGAATCAATTGAGCCAGCGATGCGATGCCTGGCAATGCCAAATATGGGGAACCggtg   <  2:667872‑M2/73‑1 (MQ=255)                                                                        tgaTTAGGTAACGCTTATTTGAATCAATTGAGCCAGCGATGCGATGCCTGGCAATGCCAAATATGGGGAACCggtg   >  1:389081‑M2/4‑76 (MQ=255)                                                                         gaTTAGGTAACGCTTATTTGAATCAATTGAGCCAGCGATGCGATGCCTGGCAATGCCAAATATGGGGAaccggctc  >  2:960746‑M2/3‑73 (MQ=255)                                                                         gaTTAGGTAACGCTTATTTGAATCAATTGAGCCAGCGATGCGATGCCTGGCAATGCCAAATATGGGGAACCggtgg  >  2:829098‑M2/3‑76 (MQ=255)                                                                         gaTTAGGTAACGCTTATTTGAATCAATTGAGCCAGCGATGCGATGCCTGGCAATGCCAAATATGGGGAACCggtgg  >  2:1198924‑M2/3‑76 (MQ=255)                                                                           |                                                                          CTGCGGGTCTGAATGCGACGTTAAATACCGGCGAACACCAGGTGCAAATCAGTAATGGTGTTCCGTTAGGCACATTAGGTAACGCTTATTTGAATCAATTGAGCCAGCGATGCGATGCCTGGCAATGCCAAATATGGGGAACCGGTGG  >  REL606/1290871‑1291018

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑