breseq  version 0.33.1  revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
RA 547,694 A→G intergenic (+123/‑1156) fdrA → / → ylbF acyl‑CoA synthetase with NAD(P)‑bindingRossmann‑fold domain/hypothetical protein
RA 547,835 +G intergenic (+264/‑1015) fdrA → / → ylbF acyl‑CoA synthetase with NAD(P)‑bindingRossmann‑fold domain/hypothetical protein
RA 556,858 A→T L36Q (CTG→CAG)  folD ← bifunctional 5,10‑methylene‑tetrahydrofolatedehydrogenase/5,10‑methylene ‑tetrahydrofolatecyclohydrolase
MC JC 654,214 Δ1,199 bp insH_12 insH_12
RA 987,574 G→T intergenic (‑170/+433) ompF ← / ← asnS outer membrane porin 1a (Ia;b;F)/asparaginyl tRNA synthetase
RA 1,093,686 T→C V130A (GTA→GCA)  ycdT → diguanylate cyclase
RA 1,197,797 C→T H366H (CAC→CAT icd → isocitrate dehydrogenase,‑specific for NADP+
RA 1,197,809 C→T T370T (ACC→ACT icd → isocitrate dehydrogenase,‑specific for NADP+
RA 1,197,822 T→C L375M (TTA→CTG)  icd → isocitrate dehydrogenase,‑specific for NADP+
RA 1,197,824 A→G L375M (TTA→CTG icd → isocitrate dehydrogenase,‑specific for NADP+
RA 1,197,854 C→T N385N (AAC→AAT icd → isocitrate dehydrogenase,‑specific for NADP+
RA 1,197,857 G→C A386A (GCG→GCC icd → isocitrate dehydrogenase,‑specific for NADP+
MC JC 1,301,041 Δ1,336 bp insC_8insD_10 insC_8, insD_10
RA 1,519,222 G→T V40V (GTG→GTT ydcX → inner membrane protein
RA 1,669,601 Δ1 bp coding (678/1221 nt) dgsA ← DNA‑binding transcriptional repressor
RA 1,979,957 G→T intergenic (‑37/+398) flhD ← / ← insH_10 DNA‑binding transcriptional dual regulator/IS5 element protein
RA 2,005,401 C→T E115K (GAA→AAA)  fliC ← flagellar filament structural protein
MC JC 2,176,983 Δ1,199 bp insH_11 insH_11
RA 2,245,455 A→G S283P (TCG→CCG)  yeiB ← inner membrane protein
JC 2,288,259 (TTATCGCCT)2→3 coding (550/1761 nt) yejM → hydrolase, inner membrane
RA 2,289,601 C→A noncoding (57/77 nt) proL → tRNA‑Pro
MC JC 2,320,795 Δ1,336 bp insD_9insC_5 insD_9, insC_5
RA 2,409,829 C→A intergenic (‑87/+544) nuoA ← / ← lrhA NADH:ubiquinone oxidoreductase, membrane subunitA/DNA‑binding transcriptional repressor
RA 2,468,335 (C)9→10 intergenic (+243/‑123) fadL → / → yfdF long‑chain fatty acid outer membranetransporter/hypothetical protein
RA 2,760,998 G→A T400I (ACT→ATT)  yfjK ← hypothetical protein
RA 2,866,111 A→G intergenic (‑21/+159) rpoS ← / ← nlpD RNA polymerase, sigma S (sigma 38) factor/outer membrane lipoprotein
RA 3,268,825 A→G T186A (ACA→GCA)  yhaC → hypothetical protein
RA 3,449,011 A→C I774S (ATC→AGC)  rpoC ← RNA polymerase, beta prime subunit
RA 3,666,710 G→T P314Q (CCG→CAG)  wzzE ← Entobacterial Common Antigen (ECA)polysaccharide chain length modulation protein
MC JC 3,742,936 Δ1,336 bp insD_8insC insD_8, insC
MC JC 3,746,911 Δ1,199 bp insH_13 insH_13
MC JC 3,750,591 Δ1,199 bp insH_14 insH_14
MC JC 3,958,123 Δ34 bp coding (1156‑1189/1287 nt) dctA → C4‑dicarboxylic acid, orotate and citratetransporter
RA 4,291,189 G→A intergenic (‑228/‑165) acs ← / → nrfA bifunctional acetyl‑CoA synthetase/propionyl‑CoAsynthetase/nitrite reductase, formate‑dependent,cytochrome
MC JC 4,310,363 Δ1,199 bp insH_15[alsK] insH_15, [alsK]
RA 4,371,274 (A)5→3 intergenic (‑9/+296) dcuA ← / ← aspA C4‑dicarboxylate antiporter/aspartate ammonia‑lyase

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_007779_cfb 1197860 1213111 15252 31 [29] [29] 30 [icd]–mcrA [icd],ymfD,ymfE,lit,intE,xisE,ymfH,ymfI,ymfJ,cohE,croE,ymfL,ymfM,ymfN,ymfR,ymfO,ymfP,ymfQ,stfP,tfaP,tfaE,pinE,mcrA
* * ÷ NC_007779_cfb 1396609 1401726 5118 96 [0] [2] 66 [ynaI]–[ogt] [ynaI],insH_8,ynaJ,uspE,fnr,[ogt]
* * ÷ NC_007779_cfb 3975089 3988254–3987180 12092–13166 82 [0] [28] 30 [gadX]–insH [gadX],gadW,mdtF,mdtE,gadE,hdeD,hdeA,hdeB,yhiD,dctR,slp,insH

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_007779_cfb 380484 =NA (NA)117 (1.470) 98/438 0.0 NA intergenic (‑418/‑46) yaiO/insC_2 hypothetical protein/IS2 insertion element repressor InsA
?NC_007779_cfb = 381407 NA (NA)coding (510/906 nt) insD IS2 insertion element transposase InsAB'
* ? NC_007779_cfb 381401 =NA (NA)89 (1.130) 82/434 0.1 NA coding (504/906 nt) insD IS2 insertion element transposase InsAB'
?NC_007779_cfb = 381812 NA (NA)coding (303/387 nt) yaiF hypothetical protein
* ? NC_007779_cfb = 13966080 (0.000)95 (1.190) 82/438 0.1 100% coding (1029/1032 nt) ynaI inner membrane protein
?NC_007779_cfb 1397758 = NA (NA)intergenic (‑122/‑32) ynaI/insH_8 inner membrane protein/IS5 transposase and trans‑activator
* ? NC_007779_cfb 1401727 =2 (0.030)64 (0.800) 56/438 0.7 97.0% coding (224/516 nt) ogt O‑6‑alkylguanine‑DNA:cysteine‑proteinmethyltransf erase
?NC_007779_cfb 1980209 = NA (NA)intergenic (‑289/+146) flhD/insH_10 DNA‑binding transcriptional dual regulator/IS5 element protein
* ? NC_007779_cfb = 3186081NA (NA)72 (0.910) 58/436 0.6 98.6% coding (26/2466 nt) yqiG outer membrane usher protein
?NC_007779_cfb 3512753 = 1 (0.010)coding (529/1026 nt) cytR DNA‑binding transcriptional dual regulator
* ? NC_007779_cfb = 32611331 (0.010)78 (1.000) 70/430 0.2 99.4% intergenic (‑59/‑69) tdcE/insH_16 pyruvate formate‑lyase 4/2‑ketobutyrateformate‑lyase/IS5 element protein
?NC_007779_cfb 3262333 = 0 (0.000)coding (1183/1296 nt) tdcD propionate kinase/acetate kinase C, anaerobic
* ? NC_007779_cfb = 35127592 (0.030)56 (0.700) 52/436 0.9 96.6% coding (535/1026 nt) cytR DNA‑binding transcriptional dual regulator
?NC_007779_cfb = 3744262 NA (NA)intergenic (‑90/+31) insC/yidZ IS2 element protein/DNA‑binding transcriptional regulator
* ? NC_007779_cfb = 3744263NA (NA)118 (1.480) 107/438 0.0 100% intergenic (‑91/+30) insC/yidZ IS2 element protein/DNA‑binding transcriptional regulator
?NC_007779_cfb 3903274 = 0 (0.000)coding (37/825 nt) bax hypothetical protein
* ? NC_007779_cfb = 39032794 (0.050)104 (1.310) 85/436 0.1 96.3% coding (42/825 nt) bax hypothetical protein
?NC_007779_cfb = 4504190 NA (NA)intergenic (+10/‑89) insD_3/yjgW IS2 insertion element transposase InsAB'/hypothetical protein
* ? NC_007779_cfb = 39750880 (0.000)82 (1.030) 71/438 0.2 100% coding (484/825 nt) gadX DNA‑binding transcriptional dual regulator
?NC_007779_cfb 3987185 = NA (NA)intergenic (‑731/‑32) slp/insH outer membrane lipoprotein/IS5 transposase and trans‑activator
* ? NC_007779_cfb = 4311556NA (NA)88 (1.110) 67/436 0.3 97.8% coding (922/1029 nt) alsK D‑allose kinase
?NC_007779_cfb 4540915 = 2 (0.030)intergenic (+204/+379) yjhR/nanS frameshift suppressor/hypothetical protein