breseq  version 0.33.1  revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Marginal read alignment evidence (highest frequency 20 of 40 shown, sorted by frequency from high to low)
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0077791,097,5800CT53.6% 8.3 / 97.1 69intergenic (+329/+45)Y75_RS05375/Y75_RS05385hypothetical protein/RtT sRNA
*NC_0077791,097,5400CA39.2% 97.2 / 67.8 74intergenic (+289/+85)Y75_RS05375/Y75_RS05385hypothetical protein/RtT sRNA
*NC_0077793,205,0910CT34.3% 104.4 / 34.7 67intergenic (‑179/‑28)ttdR/ttdADNA‑binding transcriptional activator TtdR/L(+)‑tartrate dehydratase subunit alpha
*NC_0077792,902,3480CA32.4% 235.2 / 55.2 105intergenic (+318/+1055)Y75_RS14470/queEkinase/7‑carboxy‑7‑deazaguanine synthase QueE
*NC_0077794,291,3090TA30.1% 150.5 / 33.0 73intergenic (‑348/‑45)acs/nrfAacetate‑‑CoA ligase/ammonia‑forming nitrite reductase cytochrome c552 subunit
*NC_00777929,4860CA28.4% 178.2 / 33.5 74intergenic (+291/‑165)dapB/Y75_RS001654‑hydroxy‑tetrahydrodipicolinate reductase/glutamine‑hydrolyzing carbamoyl‑phosphate synthase small subunit
*NC_0077792,004,0550AC28.0% 171.4 / 12.6 75intergenic (‑129/+192)Y75_RS10125/fliCRNA polymerase sigma factor FliA/H48 family flagellin FliC
*NC_0077793,404,0730TG27.8% 152.3 / 16.8 72G245G (GGT→GGGY75_RS16905oxidoreductase
*NC_0077794,609,0240CT27.8% 182.0 / 36.3 90S62F (TCT→TTT) bglJDNA‑binding transcriptional activator BglJ
*NC_0077794,291,3000GA27.5% 129.3 / 28.1 69intergenic (‑339/‑54)acs/nrfAacetate‑‑CoA ligase/ammonia‑forming nitrite reductase cytochrome c552 subunit
*NC_0077792,553,4880TG27.4% 125.8 / 11.9 62V457G (GTT→GGT) murPPTS N‑acetylmuramic acid transporter subunit IIBC
*NC_007779753,5470TA26.4% 201.9 / 29.1 87intergenic (‑330/+60)Y75_RS03720/gltAtype 1 fimbrial protein/citrate (Si)‑synthase
*NC_0077793,205,1020CA25.8% 128.8 / 14.9 66intergenic (‑190/‑17)ttdR/ttdADNA‑binding transcriptional activator TtdR/L(+)‑tartrate dehydratase subunit alpha
*NC_0077792,902,3420GT24.8% 319.1 / 17.1 109intergenic (+312/+1061)Y75_RS14470/queEkinase/7‑carboxy‑7‑deazaguanine synthase QueE
*NC_0077792,902,3710GA24.8% 213.8 / 55.4 101intergenic (+341/+1032)Y75_RS14470/queEkinase/7‑carboxy‑7‑deazaguanine synthase QueE
*NC_0077793,189,7590CT24.7% 235.1 / 15.6 98P158S (CCA→TCA) Y75_RS15860fimbrial protein
*NC_0077794,291,3060CA24.7% 186.3 / 13.8 73intergenic (‑345/‑48)acs/nrfAacetate‑‑CoA ligase/ammonia‑forming nitrite reductase cytochrome c552 subunit
*NC_00777929,4960TA24.4% 187.2 / 23.2 78intergenic (+301/‑155)dapB/Y75_RS001654‑hydroxy‑tetrahydrodipicolinate reductase/glutamine‑hydrolyzing carbamoyl‑phosphate synthase small subunit
*NC_0077792,488,7000GA23.2% 276.2 / 24.0 112S29L (TCA→TTA) emrKmultidrug efflux MFS transporter periplasmic adaptor subunit EmrK
*NC_0077792,004,0480GA23.0% 166.3 / 17.5 74intergenic (‑122/+199)Y75_RS10125/fliCRNA polymerase sigma factor FliA/H48 family flagellin FliC

Marginal new junction evidence (sorted from low to high skew)
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_007779 4547362 =114 (0.960)15 (0.130) 14/478 13.4 11.2% intergenic (+49/‑433) fimE/fimA type 1 fimbria regulatory protein FimE/metal ABC transporter substrate‑binding lipoprotein/fibrin‑binding adhesin FimA
?NC_007779 4547676 = 127 (1.110)intergenic (+363/‑119) fimE/fimA type 1 fimbria regulatory protein FimE/metal ABC transporter substrate‑binding lipoprotein/fibrin‑binding adhesin FimA
* ? NC_007779 = 4547370113 (0.960)10 (0.090) 10/478 15.4 7.8% intergenic (+57/‑425) fimE/fimA type 1 fimbria regulatory protein FimE/metal ABC transporter substrate‑binding lipoprotein/fibrin‑binding adhesin FimA
?NC_007779 = 4547666 126 (1.110)intergenic (+353/‑129) fimE/fimA type 1 fimbria regulatory protein FimE/metal ABC transporter substrate‑binding lipoprotein/fibrin‑binding adhesin FimA