breseq  version 0.32.0b  
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
RA 753,013 G→T E551* (GAA→TAA)  sdhA → succinate dehydrogenase, flavoprotein subunit
RA 2,718,678 C→G G143A (GGC→GCC)  kgtP ← alpha‑ketoglutarate transporter
RA 2,719,426 T→C intergenic (‑321/+2) kgtP ← / ← rrfG alpha‑ketoglutarate transporter/5S ribosomal RNA of rrnG operon
RA 2,861,917 G→A Q66* (CAG→TAG)  nlpD ← activator of AmiC murein hydrolase activity, lipoprotein
RA 2,870,339 G→A G134S (GGC→AGC)  iap → aminopeptidase in alkaline phosphatase isozyme conversion
RA 3,019,631 G→T M169I (ATG→ATT guaD → guanine deaminase
RA 3,029,079 C→T A188T (GCT→ACT)  prfB ← peptide chain release factor RF‑2
RA 3,204,161 G→A E8K (GAA→AAA)  rpsU → 30S ribosomal subunit protein S21
RA 4,176,029 C→A P251Q (CCG→CAG)  rpoC → RNA polymerase, beta prime subunit
RA 4,183,494 C→A R4L (CGT→CTT)  thiF ← adenylyltransferase, modifies ThiS C‑terminus
RA 4,390,205 G→T G34V (GGG→GTG)  hfq → global sRNA chaperone; HF‑I, host factor for RNA phage Q beta replication

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ CP009273 345704 346929 1226 23 [21] [21] 22 prpC–[prpD] prpC, [prpD]
* * ÷ CP009273 748611 749983 1373 22 [21] [21] 22 gltA gltA

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? CP009273 360104 =NA (NA)106 (1.970) 69/290 0.0 NA intergenic (‑38/+557) lacZ/lacI pseudogene, truncated/DNA‑binding transcriptional repressor
?CP009273 = 360286 NA (NA)intergenic (‑220/+375) lacZ/lacI pseudogene, truncated/DNA‑binding transcriptional repressor
* ? CP009273 1203246 =9 (0.170)43 (0.900) 38/258 0.3 86.0% coding (290/630 nt) stfP e14 prophage; uncharacterized protein
?CP009273 1205075 = 6 (0.130)pseudogene (1/501 nt) stfE pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related
* ? CP009273 = 12032619 (0.170)49 (1.020) 39/258 0.2 87.5% coding (305/630 nt) stfP e14 prophage; uncharacterized protein
?CP009273 = 1205058 6 (0.130)pseudogene (18/501 nt) stfE pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related
* ? CP009273 = 1391495NA (NA)47 (0.870) 42/290 0.3 95.9% intergenic (+146/‑127) insH1/ynaJ IS5 transposase and trans‑activator/DUF2534 family putative inner membrane protein
?CP009273 = 1972751 2 (0.040)intergenic (‑297/‑483) flhD/uspC flagellar class II regulon transcriptional activator, with FlhC/universal stress protein
* ? CP009273 1972748 =2 (0.040)63 (1.180) 38/288 0.5 96.9% intergenic (‑294/‑486) flhD/uspC flagellar class II regulon transcriptional activator, with FlhC/universal stress protein
?CP009273 = 2283592 NA (NA)noncoding (1/1195 nt) IS5 repeat region
* ? CP009273 2269338 =3 (0.060)44 (0.820) 39/290 0.4 93.6% coding (587/1026 nt) yejE microcin C transporter YejABEF, permease subunit; ABC family
?CP009273 = 3577555 NA (NA)intergenic (+15/‑1) insB1/yrhA IS1 transposase B/pseudogene, interrupted by IS1E
* ? CP009273 = 22693466 (0.110)53 (0.980) 44/290 0.2 89.8% coding (595/1026 nt) yejE microcin C transporter YejABEF, permease subunit; ABC family
?CP009273 3576788 = NA (NA)intergenic (+10/‑55) yrhA/insA pseudogene, interrupted by IS1E/IS1 repressor TnpA