breseq  version 0.32.0b  
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation freq annotation gene description
RA 468,144 Δ1 bp 27.8% coding (91/339 nt) glnK → nitrogen assimilation regulatory protein for GlnL, GlnE, and AmtB
JC JC 542,271 IS5 (–) +4 bp 42.9% coding (136‑139/1668 nt) fdrA → putative NAD(P)‑binding acyl‑CoA synthetase
RA 894,431 Δ1 bp 13.8% coding (458/1128 nt) rlmC → 23S rRNA m(5)U747 methyltransferase, SAM‑dependent
RA 1,402,027 A→G 17.4% L9P (CTG→CCG)  ydaM ← diguanylate cyclase, csgD regulator
RA 1,909,455 G→A 38.8% Q113* (CAG→TAG)  proQ ← RNA chaperone, putative ProP translation regulator
JC JC 2,400,257 IS5 (+) +4 bp 63.6% intergenic (‑137/‑780) lrhA ← / → alaA transcriptional repressor of flagellar, motility and chemotaxis genes/valine‑pyruvate aminotransferase 2
JC 2,420,337 Δ120 bp 88.2% intergenic (‑70/+32) hisJ ← / ← argT histidine/lysine/arginine/ornithine transporter subunit/lysine/arginine/ornithine transporter subunit
RA 2,719,426 T→C 27.4% intergenic (‑321/+2) kgtP ← / ← rrfG alpha‑ketoglutarate transporter/5S ribosomal RNA of rrnG operon
RA 2,787,133 A→G 21.0% D347G (GAC→GGC)  gabT → 4‑aminobutyrate aminotransferase, PLP‑dependent
RA 2,985,002 G→T 100% D126Y (GAT→TAT)  ygeG → SycD‑like chaperone family TPR‑repeat‑containing protein
JC JC 3,178,254 IS5 (+) +4 bp 55.6% coding (56‑59/291 nt) yqiC → BMFP family putative fusogenic protein
RA 4,089,622 A→G 11.5% W278R (TGG→CGG)  rhaT ← L‑rhamnose:proton symporter
RA 4,390,190 G→C 25.0% G29A (GGT→GCT)  hfq → global sRNA chaperone; HF‑I, host factor for RNA phage Q beta replication

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? CP009273 = 287112NA (NA)9 (0.500) 9/270 NT 33.3% noncoding (1/768 nt) IS1 repeat region
?CP009273 = 2269346 18 (1.000)coding (595/1026 nt) yejE microcin C transporter YejABEF, permease subunit; ABC family
* ? CP009273 360104 =NA (NA)39 (2.160) 28/270 NT NA intergenic (‑38/+557) lacZ/lacI pseudogene, truncated/DNA‑binding transcriptional repressor
?CP009273 = 360286 NA (NA)intergenic (‑220/+375) lacZ/lacI pseudogene, truncated/DNA‑binding transcriptional repressor
* ? CP009273 454243 =6 (0.330)10 (0.570) 8/264 NT 63.0% intergenic (+87/‑101) clpX/lon ATPase and specificity subunit of ClpX‑ClpP ATP‑dependent serine protease/DNA‑binding ATP‑dependent protease La
?CP009273 603467 = NA (NA)intergenic (+23/‑54) hokE/insL1 toxic polypeptide, small/IS186 transposase
* ? CP009273 = 4542555 (0.280)6 (0.340) 6/262 NT 55.3% intergenic (+99/‑89) clpX/lon ATPase and specificity subunit of ClpX‑ClpP ATP‑dependent serine protease/DNA‑binding ATP‑dependent protease La
?CP009273 = 604803 NA (NA)intergenic (+170/+112) insL1/entD IS186 transposase/phosphopantetheinyltransferase component of enterobactin synthase multienzyme complex
* ? CP009273 = 1391495NA (NA)6 (0.330) 6/270 NT 42.9% intergenic (+146/‑127) insH1/ynaJ IS5 transposase and trans‑activator/DUF2534 family putative inner membrane protein
?CP009273 = 1972751 8 (0.440)intergenic (‑297/‑483) flhD/uspC flagellar class II regulon transcriptional activator, with FlhC/universal stress protein
* ? CP009273 1972748 =8 (0.450)4 (0.220) 4/268 NT 33.3% intergenic (‑294/‑486) flhD/uspC flagellar class II regulon transcriptional activator, with FlhC/universal stress protein
?CP009273 = 2283592 NA (NA)noncoding (1/1195 nt) IS5 repeat region
* ? CP009273 2269338 =18 (1.000)11 (0.610) 11/270 NT 37.9% coding (587/1026 nt) yejE microcin C transporter YejABEF, permease subunit; ABC family
?CP009273 = 3577555 NA (NA)intergenic (+15/‑1) insB1/yrhA IS1 transposase B/pseudogene, interrupted by IS1E
* ? CP009273 4532499 =9 (0.500)5 (0.300) 5/252 NT 36.5% intergenic (+49/‑433) fimE/fimA tyrosine recombinase/inversion of on/off regulator of fimA/major type 1 subunit fimbrin (pilin)
?CP009273 4532813 = 9 (0.540)intergenic (+363/‑119) fimE/fimA tyrosine recombinase/inversion of on/off regulator of fimA/major type 1 subunit fimbrin (pilin)
* ? CP009273 = 453250710 (0.560)7 (0.420) 7/252 NT 43.3% intergenic (+57/‑425) fimE/fimA tyrosine recombinase/inversion of on/off regulator of fimA/major type 1 subunit fimbrin (pilin)
?CP009273 = 4532803 9 (0.540)intergenic (+353/‑129) fimE/fimA tyrosine recombinase/inversion of on/off regulator of fimA/major type 1 subunit fimbrin (pilin)