breseq  version 0.32.0b  
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
RA 468,144 Δ1 bp coding (91/339 nt) glnK → nitrogen assimilation regulatory protein for GlnL, GlnE, and AmtB
RA 2,719,426 T→C intergenic (‑321/+2) kgtP ← / ← rrfG alpha‑ketoglutarate transporter/5S ribosomal RNA of rrnG operon
RA 2,860,429 Δ1 bp coding (482/993 nt) rpoS ← RNA polymerase, sigma S (sigma 38) factor
RA 2,985,002 G→T D126Y (GAT→TAT)  ygeG → SycD‑like chaperone family TPR‑repeat‑containing protein

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ CP009273 345689 346960 1272 66 [65] [64] 66 prpC–[prpD] prpC, [prpD]
* * ÷ CP009273 683366–684507 684509 3–1144 66 [65] [0] 155 insH1 insH1
* * ÷ CP009273 748582 749976 1395 67 [65] [64] 67 gltA gltA

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? CP009273 360104 =NA (NA)186 (1.550) 116/294 0.0 NA intergenic (‑38/+557) lacZ/lacI pseudogene, truncated/DNA‑binding transcriptional repressor
?CP009273 = 360286 NA (NA)intergenic (‑220/+375) lacZ/lacI pseudogene, truncated/DNA‑binding transcriptional repressor
* ? CP009273 = 683479NA (NA)107 (0.920) 77/284 0.4 100% noncoding (1023/1195 nt) IS5 repeat region
?CP009273 684510 = 0 (0.000)intergenic (‑41/+289) insH1/lnt IS5 transposase and trans‑activator/apolipoprotein N‑acyltransferase
* ? CP009273 = 1391495NA (NA)118 (0.980) 82/294 0.3 97.5% intergenic (+146/‑127) insH1/ynaJ IS5 transposase and trans‑activator/DUF2534 family putative inner membrane protein
?CP009273 = 1972751 3 (0.030)intergenic (‑297/‑483) flhD/uspC flagellar class II regulon transcriptional activator, with FlhC/universal stress protein
* ? CP009273 1972748 =7 (0.060)122 (1.020) 85/292 0.2 94.6% intergenic (‑294/‑486) flhD/uspC flagellar class II regulon transcriptional activator, with FlhC/universal stress protein
?CP009273 = 2283592 NA (NA)noncoding (1/1195 nt) IS5 repeat region
* ? CP009273 2269338 =5 (0.040)107 (0.890) 76/294 0.5 95.5% coding (587/1026 nt) yejE microcin C transporter YejABEF, permease subunit; ABC family
?CP009273 = 3577555 NA (NA)intergenic (+15/‑1) insB1/yrhA IS1 transposase B/pseudogene, interrupted by IS1E
* ? CP009273 = 22693461 (0.010)115 (0.960) 92/294 0.1 99.1% coding (595/1026 nt) yejE microcin C transporter YejABEF, permease subunit; ABC family
?CP009273 3576788 = NA (NA)intergenic (+10/‑55) yrhA/insA pseudogene, interrupted by IS1E/IS1 repressor TnpA