Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC JC	NC_000913	4,049,912	IS2 (+) +5 bp	noncoding (14‑18/109 nt)	spf →	Spot 42 sRNA antisense regulator of galK translation, Hfq‑dependent

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	3186096 =	NA (NA)	40 (0.990)	35/284	0.2	100%	noncoding (1/1331 nt)	IS2	repeat region
	?	NC_000913	= 4049916	0 (0.000)					noncoding (18/109 nt)	spf	Spot 42 sRNA antisense regulator of galK translation, Hfq‑dependent
*	?	NC_000913	4049912 =	0 (0.000)	42 (1.040)	36/284	0.1	100%	noncoding (14/109 nt)	spf	Spot 42 sRNA antisense regulator of galK translation, Hfq‑dependent
	?	NC_000913	= 4499511	NA (NA)					noncoding (1331/1331 nt)	IS2	repeat region

TTCCTGTGTGGTACGCCGTCTGCGTTTCTCCGGCCCTAAGACATCAATCATCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTA‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_000913/3186237‑3186096 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑CTTACCTCTGTACCCTACGCGACTAACTTTACTCTTTTTTGTTCAGTTCAGAAAGCACTTTTTGTAATTTTTTTTCAGTTGTTGCATAGGAATTTCTAATAGCCATCACAAAACGTGACAGCTTATGTTGCTTACTTACGAA  <  NC_000913/4049916‑4049775                                                                                                                                                                                                                                                                                              TTCCTGTGTGGCACGCCGTCTGCGTTCCTCCGGCCCTAAGACATCAACCATCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAC                                                                                                                                               <  1:482698/143‑1         TGGTACGCCGTCTGCGTTTCTCCGGCCCTAAGACATCAGTCATCTGTTCTCCAATGACTAGTCTAAAGACTAGTATTAAGACTATCACTTATTGAAGTGAGATTTGTTGTCTGGAGATTCAGGGGGCCAGTCTACTTACCTCT                                                                                                                                       >  1:399428/1‑143            tccggCGTCTGCGTTCCTCCGGCCCTAAGACATCAATCATCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTACTTACCTCTGTA                                                                                                                                    <  2:170877/138‑1                        GTTTCTCCGGCCCTAAGACATCAATCATCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTACTTACCTCTGTACCCTACGCGACT                                                                                                                        <  2:86020/143‑1                        GTTTCTCCGGCCCTAAGACATCAATCATCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTACTTACCTCTGTACCCTACGCGACT                                                                                                                        >  1:391757/1‑143                         TTTCTCCGGCCCTAAGACATCAATCATCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATCTAAGTGATATTGATTGTCTGGAGATTCAGGGGGCCAGTCTACTTACCTCTGTACCCTCCGCGACTA                                                                                                                       >  2:333297/1‑143                            CTCCGGCCCTAAGACATCAATCATCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTACTTACCTCTGTACCCTACGCGACTAACT                                                                                                                    >  1:350479/1‑143                               CGGCCCTAAGACATCAATCATCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTACTTACCTCTGTACCCTACGCGACTAACTTTA                                                                                                                 <  1:634794/143‑1                                GGCCCTAAGACATCAATCATCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTACTTACCTCTGTACCCTACGCGACTAACTTTAC                                                                                                                <  1:25786/143‑1                                 GCCCTAACACATCAATCATCTGTTCTCCGATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTACTTACCTCTGTACCCTACGCGACTAACTTTACT                                                                                                               <  2:428295/143‑1                                    CTAAGACATCAATCATCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTACTTACCTCTGTACCCTACGCGACTAACTTGACTCTT                                                                                                            >  2:494917/1‑143                                       AGACATCAATCATCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTACTTACCTCTGTACCCTACGCGCCTAACTTTCCTCTTTTT                                                                                                         >  1:634382/1‑143                                            TCAATCATCTGTTCGCCAATGACTAGTCTAAAGACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTACTTACCTCTGTACCCTACGCGACTAACTTTACTCTTTTTTGTTC                                                                                                    <  2:425245/143‑1                                              AATCATCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTACTTACCTCTGTACCCTACGCGACTAACTTTACTCTTTTTTGTTCAG                                                                                                  >  1:259381/1‑143                                                                         AAAAACGAGTGTTAAGACTATCACTTATTTAGGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTACTTACCTCTGTACCCTACGCGACTAACTTTACTCTTTTTTGTTCAGTTCGGAAAGCACTTTTTGTAATTTTTT                                                                       <  2:722266/143‑1                                                                           AAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTACTTACCTCTGTACCCTACGCGACTAACTTTACTCTTTTTTGTTCAGTTCAGAAAGCACTTTTTGTAATTTTTTTT                                                                     >  1:552981/1‑143                                                                           AAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTACTTACCTCTGTACCCTACGCGACTAACTTTACTCTTTTTTGTTCAGTTCAGAAAGCACTTTTTGTAATTTTTTTT                                                                     >  2:113056/1‑143                                                                                   ATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTACTTACCTCTGTACCCTACGCGACTAACTTTACTCTTTTTTGTTCAGTTCAGAAAGCACTCTTTGTAATTTTTTTTCTGTTGTT                                                             >  2:431772/1‑143                                                                                    TTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTACTTACCTCTGTACCCTACGCGACTAACTTTACTCTTTTTTGTTCAGTTCAGAAAGCACTTTTTGTAATTTTTTTTCAATTCTTG                                                            >  1:447423/1‑143                                                                                       AGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTACTTACCTCTGTACCCTACGCGACTAACTTTACTCTTTTTTGTTCAGTTCAGAAAGCACTTTTTGTAATTTTTTTTCAGTTGTTGCAT                                                         >  1:560764/1‑143                                                                                             TCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTACTTACCTCTGTACCCTACGCGACTAACTTTACTCTTTTTTGTTCAGTTCAGAAAGCACTTTTTGTAATTTTTTTTCAGTTGTTGCATAGGAAT                                                   <  2:391757/143‑1                                                                                               ACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTACTTACCTCTGTACCCTACGCGACTAACTTTACTCTTTTTTGTTCAGTTCAGAAAGCACTTTTTGTAATTTTTTTTCAGTTGTTGCATAGGAATTT                                                 >  2:203314/1‑143                                                                                                 TTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTACTTACCTCTGTACCCTACGCGACTAACTTTACTCTTTTTTGTTCAGTTCAGAAAGCACTTTTTGTAATTTTTTTTCAGTTGTTGCATAGGAATTTCT                                               >  1:656346/1‑143                                                                                                              ATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTACTTACCTCTGTACCCTACGCGACTAACTTTACTCTTTTTTGTTCAGTTCAGAAAGCACTTTTTGTAATTTTTTTTCAGTTGTTGTATAGGAATTTCTCATTGCCATCACA                                  >  2:459777/1‑143                                                                                                                 GGTTGTCTGGAGATTCAGGGGGCCAGTCTACTTACCTCTGTACCCTACGCGACTAACTTTTCTCTTTTTTGTTCAGTTCAGAAAGCACTTTTTGTAATTTTTTTTCAGTTGTTGCATAGGAATTTCTAATAGCCATCACAAAA                               <  1:533252/143‑1                                                                                                                    TGTCTGGAGATTCAGGGGGCCAGTCTACTTACCTCTGTACCCTACGCGACTAACTTTACTCTTTTTTGTTCAGTTCAGAAAGCACTTTTTGTAATTTTTTTTCAGTTGTTGCATAGGAATTTCTAATAGCCATCACAAAACGT                            <  1:441547/143‑1                                                                                                                        TGGAGATTCAGGGGGCCAGTCTACTTACCTCTGTACCCTACGCGACTAACTTTACTCTTTTTTGTTCAGTTCAGAAAGCACTTTTTGTAATTTTTTTTCATTTGTTGCATAGGATTTTCTATTCGCCATCCCAAAACGTGACc                        >  2:688129/1‑142                                                                                                                               TCAGGGGGCCAGTCTACTTACCTCTGTACCCTACGCGACTAACTTTACTCTTTTTTGTTCAGTTCAGAAAGCACTTTTTGTAATTTTTTTTCAGTTGTTGCATAGCAATTTCTAATAGCCATCACAAAACGTGCCAGCTTATG                 >  2:216542/1‑143                                                                                                                                 AGGGGGCCAGTCTACTTACCTCTGTACCCTACGCGACTAACTTTACTCTTTTTTGTTCAGTTCAGAAAGCACTTTTTGTAATTTTTTTTCAGTTGTTGCATAGGAATTTCTAATAGCCATCACAAAACGTGCCAGCTTATGTT               >  1:255520/1‑143                                                                                                                                  GGGCGCCAGTCTACTTACCTCTGTACCCTACGCGACTAACTTTACTCTTTTTTGTTCAGTTCAGAAAGCACTTTTTGTAATTTTTTTTCAGTTGTTGCATAGGAATTTCTAATAGCCATCACAAAACGTGACAGCTTATGTTG              <  1:46945/143‑1                                                                                                                                    GGGCCAGTCTGCTTACGTCTGTCCCCTACGCGACTAACTTCATTCTTTTTTGTTCAGTTCAGAAAGCACTTTTTGTACTTTTTTTTCAGTTGTTGCATAGGAATTTCTAATAGCCATCACAAAACGTGACAGCTTATGTTACT            <  2:627871/143‑1                                                                                                                                        CAGTCTACTTACCTCTGTACCCTACGCGACTAACTTTACTCTTTTTTGTTCAGTTCAGAAAGCACTTTTTGTAATTTTTTTTCAGTTGTTGCATAGGAATTTCTAATAGCCATCACAAAACGTGACAGCTTATGTTGCTTACT        <  1:551864/143‑1                                                                                                                                         AGTCTACTTACCTCTGTACCCTACGCGACTAACTTTACTCTTTTTTGTTCAGTTCAGAAAGCACTTTTTGTAATTTTTTTTCAGTTGTTGTATAGGAATTTCTAATAGCCATCACAAAACGTTACAGCTTTTTTTGCTTACTT       >  2:35943/1‑143                                                                                                                                         AGTCTACTTACCTCTGTACCCTACGCGACTAACTTTACTCTTTTTTGTTCAGTTCAGAAAGCACTTTTTGTAATTTTTTTTCAGTTGTTGCATAGGAATTTCTAATAGCCATCACAAAACGTGACAGCTTATGTTGCTTACTT       >  1:693320/1‑143                                                                                                                                         AGTCTACTTACCTCTGTACCCTACGCGACTAACTTTACTCTTTTTTGTTCAGTTCAGAAAGCACTTTTTGTAATTTTTTTTCAGTTGTTGCATAGGAATTTCTAATAGCCATCACAAAACGTGACAGCTTATG                 <  2:324877/133‑1                                                                                                                                         AGTCTACTTACCTCTGTACCCTACGCGACTAACTTTACTCTTTTTTGTTCAGTTCAGAAAGCACTTTTTGTAATTTTTTTTCAGTTGTTGCATAGGAATTTCTAATAGCCATCACAAAACGTGACAGCTTATG                 >  1:324877/1‑133                                                                                                                                          GTCTACTGTCCTTTGTCTCCTACGCGACTCACTTTGCTCGTTTTTGTTCAGTTGAGAAAGCACTTTTTGTAATGTTTTTTCAGTGGTGGCATAGGAATTTCTAATAGCCATCACAAAACGTGACAGCTTATGTTGCGTACTTA      <  2:384543/143‑1                                                                                                                                             TACTTACCTCTGTACCCTACGCGACTAACTTTACTCTTTTTTGTTCAGTTCAGAAAGCACTTTTTGTAATTTTTTTTCAGTTGTTGCATAGGAATTTCTAATATCTATCACAAAACGTGACAGCTTATGTTGGTTACTTACGA   >  2:477455/1‑143                                                                                                                                             TACTTACCTCTGTACCCTACGCGACTAACTTTACTCTTTTTTGTTCAGTTCAGAAAGCACTTTTTGTAATTTTTTTTCAGTTGTTGCATAGGAATTTCTAATAGCCATCACAAAACGTGACAGCTTATGTTGCTTACTTACGA   >  1:345525/1‑143                                                                                                                                              ACTTACCTCTGTACCCTACGCGACTAACTTTACTCTTTTTTGTTCAGTTCAGAAAGCACTTTTTGTAATTTTTTTTCAGTTGTTGCATAGGAATTTCTAATAGCCATCACAAAACGTGACAGCTTATGTTGCTTACTTACGga  >  1:575120/1‑141                                                                                                                                                                                                                                                                                              TTCCTGTGTGGTACGCCGTCTGCGTTTCTCCGGCCCTAAGACATCAATCATCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTA‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_000913/3186237‑3186096 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑CTTACCTCTGTACCCTACGCGACTAACTTTACTCTTTTTTGTTCAGTTCAGAAAGCACTTTTTGTAATTTTTTTTCAGTTGTTGCATAGGAATTTCTAATAGCCATCACAAAACGTGACAGCTTATGTTGCTTACTTACGAA  <  NC_000913/4049916‑4049775

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.