Predicted mutation
evidence seq id position mutation annotation gene description
JC JC NC_000913 4,049,912 IS2 (+) +5 bp noncoding (14‑18/109 nt) spf → Spot 42 sRNA antisense regulator of galK translation, Hfq‑dependent

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 3186096 =NA (NA)40 (0.990) 35/284 0.2 100% noncoding (1/1331 nt) IS2 repeat region
?NC_000913 = 4049916 0 (0.000)noncoding (18/109 nt) spf Spot 42 sRNA antisense regulator of galK translation, Hfq‑dependent
* ? NC_000913 4049912 =0 (0.000)42 (1.040) 36/284 0.1 100% noncoding (14/109 nt) spf Spot 42 sRNA antisense regulator of galK translation, Hfq‑dependent
?NC_000913 = 4499511 NA (NA)noncoding (1331/1331 nt) IS2 repeat region

CTTACGAGTAATCGCATTTGGCCGGGTTCGCCCGGCCACATCATTAACGGATTAATGATAAGTGGATCAGATGTATAAAAAATTAAAATTAACCACAATAAGCGAATTGATTAAAAATATTTATTGTTCATTATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGATGATTGATGTCTTAGGGCCGGAGAAACGCAGACGGCGTACCACACAG  >  NC_000913/3185955‑3186234
                                                                                                                                               |                                                                                                                                          
cTTACGAGTAATCGCATTTGGCCGGGTTCGCCCGGCCACATCATTAACGGATTAATGATAAGTGGATCAGATGTATAAAAAATTAAAATTAACCACAATAAGCGAATTGATTAAAAATATTTATTGTTCATTATCCGttatta                                                                                                                                           >  2:351915/1‑143 (MQ=255)
 ttACGAGTAATCGCATTTGGCCGGGTTCGCCCGGCCACATCATTAACGGATTAATGATAAGTGGATCAGATGTATAAAAAATTAAAATTAACCACAATAAGCGAATTGATTAAAAATATTTATTTTTCATTATACGTTATTAg                                                                                                                                          >  1:490623/1‑143 (MQ=255)
   aCGAGTAATCGCATTTGGCCGGGTTCGCCCGGCCACATCATTAACGGATTAATGATAAGTGGATCAGATGTATAAAAAATTAAAATTAACCACAATAAGCGAATTGATTAAAAATATTTATTGTTCATTATCCGTTATTAGAc                                                                                                                                        <  1:271091/143‑1 (MQ=255)
     gAGTACTCGCATTTGGCCGGGTTCGCCCGGCCACATCATTAACGGATTAATGATAAGTGGATCAGATGTATAAAAAATTAAAATTAACCACAATAAGCGAATTGATTAAAAATATTTATTGTTCATTATCCGTTATTAGACTg                                                                                                                                      <  1:45863/143‑1 (MQ=255)
       gTAATCGCATTTGGCCGGGTTCGCCCGGCCACATCATTAACGGATTAATGATAAGTGGATCAGATGTATAAAAAATTAAAATTAACCACAATAAGCGAATTGATTAAAAATATTTATTGTTCATTATCCGTTATTAGACTgac                                                                                                                                    >  1:80531/1‑141 (MQ=255)
        tAATCGCATTTTGCCGGGTTCGCCCGGCCACATCACTAACGGATTAATGATAAGTGGATCAGATGTATAAAAAATTAAAATTAACCACAATAAGCGAATTGATTAAAAATATTTATTGTTCATTATCCGTTATTAGACTGGcc                                                                                                                                   <  2:533510/143‑1 (MQ=255)
             gCATTTGGCCGGGTTCGCCCGGCCACATCATTAACGGATTAATGATAAGTGGATCAGATGTATAAAAAATTAAAATTAACCACAATAAGCGAATTGATTAAAAATATTTATTGTTCATTATCCGTTATTAGACTGGCCCCCTg                                                                                                                              >  1:581615/1‑143 (MQ=255)
                   ggCCGGGTTCGCCCGGCCACATCATTAACGGATTAATGATAAGTGGATCAGATGTATAAAAAATTAAAATTAACCACAATAAGCGAATCGATTAAAAATATTTATTGTTCATTATCCGTTATTAGACTGGCCCCCTGAAtctc                                                                                                                        <  1:379271/143‑1 (MQ=255)
                      cGGGTTCGCCCGGCCACATCATTAACGGATTAATGATAAGTGGATCAGATGTATAAAAAATTAAAATTAACCACAATAAGCGAATTGATTAAAAATATTTATTGTTCATTATCCGTTATTAGACTGGCCCCCTGAATCTCCAg                                                                                                                     >  1:677982/1‑143 (MQ=255)
                          ttCGCCCGTCCACAACAGTAACGGATTAATCATAAGTGGATCAGATGTATAAAAAATTAAAATTAACCACAATAAGCGAATTGATTAAAAATATTTATTGTTCATTATCCTTTATTAGACTGGCCCCCTGAATCTCCAGACaa                                                                                                                 <  2:382636/143‑1 (MQ=255)
                            cGCCCGGCCACATCATTAACGGATTAATGATAAGTGGATCAGATGTATAAAAAATTAAAATTAACCACAATAAGCGAATTGATTAAAAATATTTATTGTTCATTATCCGTTATTAGACTGGCCCCCTGAATCTCCAGCCAAcg                                                                                                               >  2:488071/1‑142 (MQ=255)
                            cGCCCGGCCACATCATTAACGGATTAATGATAAGTGGATCAGATGTATAAAAAATTAAAATTAACCACAATAAGCGAATTGATTAAAAATATTTATTGTTCATTATCCGTTATTAGACTGGCACCCTGAATCTCCAGACAAcc                                                                                                               >  1:251094/1‑143 (MQ=255)
                              cccGGCCACATCATTAACGGATTAATGATAAGTGGATCAGATGTATAAAAAATTAAAATTAACCACAATAAGCGAATAGATTAAAAATATTTATAGTTCATAATCCGTTATTAGACTGCCCCCCTGAATCTCCAGACAACCaa                                                                                                             >  2:82333/1‑143 (MQ=255)
                                cgcccACATCCTTACCGGATTAATGATAAGTGGATCCGATGCATCACAAATTACAAAAAACCCATTTAGCCGTCTTGATTAAAAATATTTATTGTCAATTATAGCATAAATGTTCTGTTCCCTGAATCTCCAGACAACCAata                                                                                                           <  2:186215/140‑1 (MQ=255)
                                  gCCACATCATTAACGGATTAATGATAAGTGGATCAGATGTATAAAAAATTAAAATTAACCACAATAAGCGAATTGATTAAAAATATTTATTGTTCATTATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATc                                                                                                         >  2:500569/1‑143 (MQ=255)
                                     acaTCATTAACGGATTAATGATAAGTGGATCAGATGTATAAAAAATTAAAATTAACCACAATAAGCGAATTGATTAAAAATATTTATTGTTCATTATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACt                                                                                                      <  2:274587/143‑1 (MQ=255)
                                        tcatTAACGGATTAATGATAAGTGGATCAGATGTATAAAAAATTAAAATTAACCACAATAAGCGAATTGATTAAAAATATTTATTGTTCATTATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTaa                                                                                                   <  2:142491/143‑1 (MQ=255)
                                            tAACGGATTAATGATAAATGGAGCAGATGTATAAAAAATCAAAATTAACCACAATAAGCGAATTGATTAAAAATATTTATTGTCCATTATCCGTTATTAAACTGGACCCCTGAATCCCCAGACAACTAACATCACCTAAATac                                                                                               >  2:91112/1‑142 (MQ=255)
                                                ggATTAATGATAAGTGGATCAGATGTATAAAAAATTAAAATTAACCACAATAAGCGAATTGATTAAAAATATTTATTGTTCATTATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGa                                                                                           <  2:193820/143‑1 (MQ=255)
                                                  aTTAGTGATAAGTGGATCAGATGTATAAAAAATTAAAATTAACCACAATAAGCGAATTGATTAAAAATATTTATTGTTCATTATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATa                                                                                         <  2:572738/143‑1 (MQ=255)
                                                         aTAAGTGGATCAGATGTATAAAAAATTAAAATTAACCACAATAAGCGAATTGATTAAAAATATTTATTGTTCATTATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTaa                                                                                  <  1:120662/143‑1 (MQ=255)
                                                          tAAGTGGATCAGATGTATAAAAAATTAAAATTAACCACAATAAGCGAATTGATTAAAAATATTTATTGTTCATTATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAAt                                                                                 <  2:653714/143‑1 (MQ=255)
                                                           aaGTGGATCAGATGTATAAAAAATTAAAATTAACCACAATAAGCGAATTGATTAAAAATATTTATTGTTCATTATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATa                                                                                <  2:581615/143‑1 (MQ=255)
                                                             gTGGATCAGATGTATAAAAAATTAAAATTAACCACAATAAGCGAATTGATTAAAAATATTTATTGTTCATTATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACt                                                                              >  2:367255/1‑143 (MQ=255)
                                                                 aTCAGATGTATAAAAAATTAAAATTAACCACAATAAGCGAATTGATTAAAAATATTTATTGTTCATTATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTGAAATAAGTGATAGTCTTAATACTAGtg                                                                          >  1:583922/1‑142 (MQ=255)
                                                                  tCAGATGTATAAAAAATTAAAATTAACCACAATAAGCGAATTGATTAAAAATATTTATTGTTCATTATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGttt                                                                         >  2:455604/1‑143 (MQ=255)
                                                                  tCAGATGTATAAAAAATTAAAATTAACCACAATAAGCGAATTGATTAAAAATATATATTGATCATTATCCGTTATTACACTGACCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTCATACtgaaat                                                                         >  2:311195/1‑138 (MQ=37)
                                                                    ggtggTATAAAAAATAAAAATTACCACAATAAGCGAATTGATTAAAAATATTTATTGTTCATTATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGtttttn                                                                      <  1:84029/139‑2 (MQ=255)
                                                                     gATGTATAAAAAATTAAAATTAACCACAATAAGCGAATTGATTAAAAATATTTATTGTTCATTATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTa                                                                      <  2:229525/143‑1 (MQ=255)
                                                                       tGTATAAAAAATTAAAATTAACCACAATAAGCGAATTGATTAAAAATATTTATTGTTCATTATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGa                                                                    <  1:173158/143‑1 (MQ=255)
                                                                       tGTATAAAAAATTAAAATTAACCACAATAAGCGAATTGATTAAAAATATTTATTGTTCATTATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGa                                                                    <  1:477663/143‑1 (MQ=255)
                                                                          ataAAAAATTAAAATTAACCACAATAAGCGAATTGATTAAAAATATTTATTGTTCATTATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTa                                                                 <  2:269583/143‑1 (MQ=255)
                                                                                 attaaaattaaCCACAATAAGCGAATTGATTAAAAATATTTATTGTTCATTATCCGTTATTAGACTGGCCCCCTGAATGTCCAGACAACCAATATCACCTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGACATTg                                                          >  2:590380/1‑143 (MQ=255)
                                                                                  ttaaaattaaCCACAATAAGCGAATTGATTAAAAATATTTATTGTTCATTATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTgg                                                         <  1:351915/143‑1 (MQ=255)
                                                                                   taaaattaaCCACAATAAGCGAATTGATTAAAAATATTTATTGTTCATTATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGga                                                        <  1:660046/143‑1 (MQ=255)
                                                                                      aattaaCCACAATAAGCGAATTGATTACAAATATATATTGTTCATTAACCATTATTAGACTCGCCCCCTGAATCTCCAGTCAACCAATATCACTTAAATTCGTGATAGTATTAATACCAGGATTTAGAACAGTCATTGgacga                                                     >  2:190141/1‑140 (MQ=255)
                                                                                      aattaaCCACAATAAGCGAATTGATTAAAAATATTTATTGTTCATTATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCCTAATACTAGTTTTTAGACTAGTCATTGGAGaa                                                     <  1:448781/143‑1 (MQ=255)
                                                                                             cacaATAAGCGAATTGATTAAAAATATTTATTGTTCATTATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAgatga                                              <  1:627672/143‑1 (MQ=255)
                                                                                              ataaTGAGCGAATAGAGTAAAAATATTTATTGTTCATTATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAgatgat                                             <  2:217879/141‑1 (MQ=255)
                                                                                                aaTAAGCGAATTGATTAAAAATATTTATTGTTCATTATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGAtgattg                                           >  1:84331/1‑143 (MQ=255)
                                                                                                  ttaGCGAATTGATCAAAAACTTTTATTGTTCATTATCCGTCATTAGTCTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGAtgattgat                                         <  2:646075/141‑1 (MQ=17)
                                                                                                   caGCGAATTGATTAAAAATATTTATTGTGCATTATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACATATGATTGATg                                        <  2:225517/142‑1 (MQ=255)
                                                                                                         aaTTGAATAAAACTATTCATTGTTCGTTATCCCTTATTAGACTGTTCCCCTCAATCTCCAGACAACCAATATCAGTTAAATAAGTGATAGTATTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGATGATTGATGTCTTa                                   <  2:239515/143‑1 (MQ=14)
                                                                                                                            ttGTTCATGATCCGCTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGATGATTGATGTCTTAGGGCCGGAGAAACGCAGAc                <  1:21310/143‑1 (MQ=255)
                                                                                                                                 cATTATCCGTTATTAGCCTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTACTACTAGTTTTTAGACTAGTCATTGGAGAACAGATGATTTATTTCTTAGGGCCGGAGAAACGCAGACGGCGt           <  2:27409/143‑1 (MQ=255)
                                                                                                                                 cATTATCCGTTATTAGACTGGCCCCCTGAATCTCCATACAACCAATATCACTTCAATAAGTGGTAGTCTTGATACTAGTTTTTAGACTAGTCATTGGAGAGAAGGCGTTCAAATTCATAGTGCCGGAGAAACGCAGACGGCGt           <  2:599559/143‑1 (MQ=14)
                                                                                                                                      tCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGGACAGATGATTGATGTCTTAGGGCCGGAGCAACGCAGTCGGCGTTCcac      >  1:97345/1‑143 (MQ=255)
                                                                                                                                           ttattaGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGATGATTGATGTCTTAGGGCCGGAGAAACGCAGACGGCGTACCACACAg  >  1:131042/1‑143 (MQ=32)
                                                                                                                                               |                                                                                                                                          
CTTACGAGTAATCGCATTTGGCCGGGTTCGCCCGGCCACATCATTAACGGATTAATGATAAGTGGATCAGATGTATAAAAAATTAAAATTAACCACAATAAGCGAATTGATTAAAAATATTTATTGTTCATTATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGATGATTGATGTCTTAGGGCCGGAGAAACGCAGACGGCGTACCACACAG  >  NC_000913/3185955‑3186234

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.