Predicted mutation
evidence seq id position mutation annotation gene description
JC JC NC_000913 4,049,912 IS2 (+) +5 bp noncoding (14‑18/109 nt) spf → Spot 42 sRNA antisense regulator of galK translation, Hfq‑dependent

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 3186096 =NA (NA)40 (0.990) 35/284 0.2 100% noncoding (1/1331 nt) IS2 repeat region
?NC_000913 = 4049916 0 (0.000)noncoding (18/109 nt) spf Spot 42 sRNA antisense regulator of galK translation, Hfq‑dependent
* ? NC_000913 4049912 =0 (0.000)42 (1.040) 36/284 0.1 100% noncoding (14/109 nt) spf Spot 42 sRNA antisense regulator of galK translation, Hfq‑dependent
?NC_000913 = 4499511 NA (NA)noncoding (1331/1331 nt) IS2 repeat region

CTTTTTTCGTAAGTAAGCAACATAAGCTGTCACGTTTTGTGATGGCTATTAGAAATTCCTATGCAACAACTGAAAAAAAATTACAAAAAGTGCTTTCTGAACTGAACAAAAAAGAGTAAAGTTAGTCGCGTAGGGTACAGAGGTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATGACTGGGGCGTTTTTTATTGGGCGAAAGAAAAGATCCGTAATGCCTGATGCGCTATGTTTATCA  >  NC_000913/4049770‑4050054
                                                                                                                                              |                                                                                                                                              
accttgcagacgcgttcgagcattataacgaatggcatccgcatagtgcgctgggttatcgctcgccacgggaatatctgcggcagcgggcttgtaatgggttaagtgataacagatgtctggaaatataggggcaaatccag                                                                                                                                                >  2:136812‑M1/143‑143 (MQ=255)
 ttttttCGTAAGGAAGCAACATAAGCCGTCACGTTGTGTGCTGGCTATTAGAAATTCCTATGCAACAACTGAAAAAAAATTACAAAAAGTGCTTTCTGAACTGAACAAAAAAGAGTAAAGTTAGTCGCGTAGGGTACAGAGGt                                                                                                                                               <  1:592026/143‑1 (MQ=255)
     tccgtAAGTAAGCAACATAAGCTGTCACGTTTTGTGATGGCTATTAGAAATTCCTATGCAACAACTGAAAAAAAATTACAAAAAGTGCTTTCTGAACTGAACAAAAAAGAGTAAAGTTAGTCGCGTAGGGTACAGAGGTAAGt                                                                                                                                           >  1:575120‑M2/3‑142 (MQ=255)
      tCGTAAGTAAGCAACATAAGCTGTCACGTTTTGTGATGGCTATTAGAAATTCCTATGCAACAACTGAAAAAAAATTACAAAAAGTGCTTTCTGAACTGAACAAAAAAGAGTAAAGTTAGTCGCGTAGGGTACAGAGGTAAGta                                                                                                                                          >  1:345525‑M2/1‑141 (MQ=255)
      tCGTAAGTAACCAACATAAGCTGTCACGTTTTGTGATAGATATTAGAAATTCCTATGCAACAACTGAAAAAAAATTACAAAAAGTGCTTTCTGAACTGAACAAAAAAGAGTAAAGTTAGTCGCGTAGGGTACAGAGGTAAGta                                                                                                                                          >  2:477455‑M2/1‑141 (MQ=255)
       aggggcggtcgagcattatatcggatggcatccgcatagtgcgctgggttatcgctcgccactggaatatctgcggcagcgggcttgtaatgggttaagtgataacagatgtctggaaatataggggcaaatccaGTAAGATg                                                                                                                                         >  2:206394‑M1/136‑143 (MQ=255)
         taagtacgCAACATAAGCTGTCACGTTTTGTGATGGCTATTAGAAATTCCTATGCCACCACTGAAAAAACATTACAAAAAGTGCTTTCTCAACTGAACAAAAACGAGCAAAGTGAGTCGCGTAGGAGACAAAGGACAGtagac                                                                                                                                       <  2:384543‑M2/143‑6 (MQ=255)
          aagtaagCAACATAAGCTGTCACGTTTTGTGATGGCTATTAGAAATTCCTATGCAACAACTGAAAAAAAATTACAAAAAGTGCTTTCTGAACTGAACAAAAAAGAGTAAAGTTAGTCGCGTAGGGTACAGAGGTAAGtagact                                                                                                                                      >  1:693320‑M2/1‑137 (MQ=255)
          aagtaagCAAAAAAAGCTGTAACGTTTTGTGATGGCTATTAGAAATTCCTATACAACAACTGAAAAAAAATTACAAAAAGTGCTTTCTGAACTGAACAAAAAAGAGTAAAGTTAGTCGCGTAGGGTACAGAGGTAAGtagact                                                                                                                                      >  2:35943‑M2/1‑137 (MQ=255)
           agtaagCAACATAAGCTGTCACGTTTTGTGATGGCTATTAGAAATTCCTATGCAACAACTGAAAAAAAATTACAAAAAGTGCTTTCTGAACTGAACAAAAAAGAGTAAAGTTAGTCGCGTAGGGTACAGAGGTAAGtagactg                                                                                                                                     <  1:551864‑M2/143‑8 (MQ=255)
            cgttcgagcattataacgaatggcattcgcatagtgcgctgggttatcgctcgccacgggaatatctgcggcagcgggcttgtaatgggttaagtgataacagatgtctggaaatataggggcaaatccaGTAAGATGTTCTa                                                                                                                                    >  2:37460‑M1/131‑143 (MQ=255)
               agTAACATAAGCTGTCACGTTTTGTGATGGCTATTAGAAATTCCTATGCAACAACTGAAAAAAAAGTACAAAAAGTGCTTTCTGAACTGAACAAAAAAGAATGAAGTTAGTCGCGTAGGGGACAGACGTAAGcagactggccc                                                                                                                                 <  2:627871‑M2/143‑12 (MQ=255)
                 gagcattataacgaatggcatccgcatagtgcgctgggttatcgctcgccacgggaatatctgcggcagcgggcttgtaatgggttaagtgataacagatgtctggaaatataggggcaaatccaGTAAGATGTTCTATCttt                                                                                                                               <  1:430376‑M1/18‑1 (MQ=255)
                 cAACATAAGCTGTCACGTTTTGTGATGGCTATTAGAAATTCCTATGCAACAACTGAAAAAAAATTACAAAAAGTGCTTTCTGAACTGAACAAAAAAGAGTAAAGTTAGTCGCGTAGGGTACAGAGGTAAGtagactggcgccc                                                                                                                               <  1:46945‑M2/143‑14 (MQ=255)
                  agcattataacgaatggcatccgcatagtgcgctgggttatcgctcgccacgggaatatctgcggcagcgggcttgtaatgggttaagtgataacagatgtctggaaatataggggcaaatccaGTAAGATGTTCTATCTTTc                                                                                                                              <  1:248720‑M1/19‑1 (MQ=255)
                  aaCATAAGCTGGCACGTTTTGTGATGGCTATTAGAAATTCCTATGCAACAACTGAAAAAAAATTACAAAAAGTGCTTTCTGAACTGAACAAAAAAGAGTAAAGTTAGTCGCGTAGGGTACAGAGGTAAGtagactggccccct                                                                                                                              >  1:255520‑M2/1‑129 (MQ=255)
                   gcattataacgaatggcatccgcatagtgcgctgggctatcgctcgccacgggaatatctgcggccgcgggcttgtactgggctcagtgataacagctgtctggaaatatcggggcagatccaGTAAGATGCTCTatcttcca                                                                                                                             <  2:265550‑M1/20‑4 (MQ=255)
                    catgaagacgaatggcatccgcatagtgcgctgggttatcgctcgccacgggaatatctgcggcagcgggcttgtaatgggttaagtgataacagatgtctggaaatataggggcaaatccaGTAAGATGTTCTATCTTTCAg                                                                                                                            >  1:83363‑M1/123‑143 (MQ=255)
                    cATAAGCTGTCACGTTTTGTGATGGCTATTAGAAATTCCTATGCAACAACTGAAAAAAAATTACAAAAAGTGCTTTCTGAACTGAACAAAAAAGAGTAAAGTTAGTCGCGTAGGGTACAGAGGTAAGtagact                                                                                                                                      <  2:324877‑M2/133‑7 (MQ=255)
                    cATAAGCTGTCACGTTTTGTGATGGCTATTAGAAATTCCTATGCAACAACTGAAAAAAAATTACAAAAAGTGCTTTCTGAACTGAACAAAAAAGAGTAAAGTTAGTCGCGTAGGGTACAGAGGTAAGtagact                                                                                                                                      >  1:324877‑M2/1‑127 (MQ=255)
                    cATAAGCTGGCACGTTTTGTGATGGCTATTAGAAATTGCTATGCAACAACTGAAAAAAAATTACAAAAAGTGCTTTCTGAACTGAACAAAAAAGAGTAAAGTTAGTCGCGTAGGGTACAGAGGTAAGtagactggccccctga                                                                                                                            >  2:216542‑M2/1‑127 (MQ=255)
                          aagggatggcagccgcatagtgcgctgggttatcgctcgccacgggaatagctgcggcagcgggcttgtaatgggttaagtgataacagatgtctggaaatataggggcaaatccaGTAAGATGTTCTATCTTTCAGACCttt                                                                                                                      >  1:52‑M1/117‑143 (MQ=255)
                          aacgaatggcatccgcatagtgcgctgggttatcgctcgccacgggaatatctgcggcagcgggcttgtaatgggttaagtgataacatatgtctggaaatataggggcaaatccaGTAAGATGTTCTATCTTTCAGACCttt                                                                                                                      >  1:379551‑M1/117‑143 (MQ=255)
                           ggtCACGTTTTGGGATGGCGAATAGAAAATCCTATGCAACAAATGAAAAAAAATTACAAAAAGTGCTTTCTGAACTGAACAAAAAAGAGTAAAGTTAGTCGCGTAGGGTACAGAGGTAAGtagactggccccctgaatctcca                                                                                                                     >  2:688129‑M2/2‑120 (MQ=255)
                               aCGTTTTGTGATGGCTATTAGAAATTCCTATGCAACAACTGAAAAAAAATTACAAAAAGTGCTTTCTGAACTGAACAAAAAAGAGTAAAGTTAGTCGCGTAGGGTACAGAGGTAAGtagactggccccctgaatctccagaca                                                                                                                 <  1:441547‑M2/143‑28 (MQ=255)
                                  ttttGTGATGGCTATTAGAAATTCCTATGCAACAACTGAAAAAAAATTACAAAAAGTGCTTTCTGAACTGAACAAAAAAGAGAAAAGTTAGTCGCGTAGGGTACAGAGGTAAGtagactggccccctgaatctccagacaacc                                                                                                              <  1:533252‑M2/143‑31 (MQ=255)
                                     tgtgATGGCAATGAGAAATTCCTATACAACAACTGAAAAAAAATTACAAAAAGTGCTTTCTGAACTGAACAAAAAAGAGTAAAGTTAGTCGCGTAGGGTACAGAGGTAAGtagactggccccctgaatctccagacaaccaat                                                                                                           >  2:459777‑M2/1‑110 (MQ=255)
                                         catggggcgctggggtatctctcgccacgggaatatctgcggcagctggcttgtcatcggtgttttaataacagatgtctggaaatataggggcaaatccaGTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCg                                                                                                       >  2:633996‑M1/102‑143 (MQ=255)
                                         catagtgcgctgggtgatcgctcgccacgggaatatctgcggcagcgggcttgtaatgggttaagtgataacagatgtctggaaatataggggcaaatccaGTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCg                                                                                                       >  1:427709‑M1/102‑143 (MQ=255)
                                                cgcgcggttatcgctcgccacgggaatatctgcggcagcgggcttggaatgggttaagtgataacagatgtctggaaatataggggcaaatccaGTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTgg                                                                                                >  1:134294‑M1/95‑143 (MQ=255)
                                                  ctgggttatcgctcgccacgggaatatctgcggcagcgggcttgtaatgggttaagtgataacagatgtctggaaatataggggcaaatccaGTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCt                                                                                              >  2:430376‑M1/93‑143 (MQ=255)
                                                  aGAAATTCCTATGCAACAACTGAAAAAAAATTACAAAAAGTGCTTTCTGAACTGAACAAAAAAGAGTAAAGTTAGTCGCGTAGGGTACAGAGGTAAGtagactggccccctgaatctccagacaaccaatatcacttaaataa                                                                                              >  1:656346‑M2/1‑97 (MQ=255)
                                                    aaaTTCCTATGCAACAACTGAAAAAAAATTACAAAAAGTGCTTTCTGAACTGAACAAAAAAGAGTAAAGTTAGTCGCGTAGGGTACAGAGGTAAGtagactggccccctgaatctccagacaaccaatatcacttaaataagt                                                                                            >  2:203314‑M2/1‑95 (MQ=255)
                                                      aTTCCTATGCAACAACTGAAAAAAAATTACAAAAAGTGCTTTCTGAACTGAACAAAAAAGAGTAAAGTTAGTCGCGTAGGGTACAGAGGTAAGtagactggccccctgaatctccagacaaccaatatcacttaaataagtga                                                                                          <  2:391757‑M2/143‑51 (MQ=255)
                                                       ttatcgctcgccacgggaatatctgcggcagcgggcttgtaatgggttaagtgataacagatgtctggaaatataggggcaaatccaGTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAata                                                                                         <  1:447215‑M1/56‑1 (MQ=255)
                                                       ttatcgctcgccacgggaatatctgcggcagcgggcttgtaatgggttaagtgataacagatgtctggaaatataggggcaaatccaGTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAAGCGGATTTGGCTGAata                                                                                         <  2:204289‑M1/56‑1 (MQ=255)
                                                        tatcgctcgccacgggaatatctgcggcagcgggcttgtaatgggttaagtgataacagatgtctggaaatataggggcaaatccaGTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCGGCTTTGGCTGAatat                                                                                        <  2:626134‑M1/57‑1 (MQ=255)
                                                         atcgctcgccacgggaatatctgcggcagcgggcttgtaatgggttaagtgataacagatgtctggaaatataggggcaaatccaGTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATAtt                                                                                       >  1:533273‑M1/86‑143 (MQ=255)
                                                            aTGCAACAACTGAAAAAAAATTACAAAAAGTGCTTTCTGAACTGAACAAAAAAGAGTAAAGTTAGTCGCGTAGGGTACAGAGGTAAGtagactggccccctgaatctccagacaaccaatatcacttaaataagtgatagtct                                                                                    >  1:560764‑M2/1‑87 (MQ=255)
                                                             ctcgccacgggaatatcggcggcagcgggcttgtaatgggttaagtgataacagatgtctggaaatataggggcaaatccaGTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAg                                                                                   >  2:42622‑M1/82‑143 (MQ=255)
                                                               caagaaTTGAAAAAAAATTACAAAAAGTGCTTTCTGAACTGAACAAAAAAGAGTAAAGTTAGTCGCGTAGGGTACAGAGGTAAGtagactggccccctgaatctccagacaaccaatatcacttaaataagtgatagtcttaa                                                                                 >  1:447423‑M2/1‑84 (MQ=255)
                                                                aacaacAGAAAAAAAATTACAAAGAGTGCTTTCTGAACTGAACAAAAAAGAGTAAAGTTAGTCGCGTAGGGTACAGAGGTAAGtagactggccccctgaatctccagacaaccaatatcacttaaataagtgatagtcttaat                                                                                >  2:431772‑M2/1‑83 (MQ=255)
                                                                 ccacgggaatatctgcggcagcgggcttgtaatgggttaagtgataacagatgtctggaaatataggggcaaatccaGTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAgccgc                                                                               <  2:139721‑M1/66‑1 (MQ=255)
                                                                  cacgggaatatctgcggcagcgggcttgtaatgggttaagtgataacagatgtctggaaatataggggcaaatccaGTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAAGCTTCTAcccgcc                                                                              <  2:239074‑M1/67‑1 (MQ=255)
                                                                      gggaggtctgcggctgcgggtttgtaatggggtaagtgttaacagatttctggatatataggggcaaatccaGTAAGATGTTCTATCTTTCAGACCTTTTACTGCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCcag                                                                          >  2:398724‑M1/73‑143 (MQ=255)
                                                                       gaatatctgcggcagcgggcttgtaatgggttaagtgataacagatgtctggaaatataggggcaaatccaGTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATGTTTTAGCCGCCCcagt                                                                         <  2:341222‑M1/72‑1 (MQ=255)
                                                                        aaaaaaaaTTACAAAAAGTGCTTTCTGAACTGAACAAAAAAGAGTAAAGTTAGTCGCGTAGGGTACAGAGGTAAGtagactggccccctgaatctccagacaaccaatatcacttaaataagtgatagtcttaatactagttt                                                                        >  1:552981‑M2/1‑75 (MQ=255)
                                                                        aaaaaaaaTTACAAAAAGTGCTTTCTGAACTGAACAAAAAAGAGTAAAGTTAGTCGCGTAGGGTACAGAGGTAAGtagactggccccctgaatctccagacaaccaatatcacttaaataagtgatagtcttaatactagttt                                                                        >  2:113056‑M2/1‑75 (MQ=255)
                                                                          aaaaaaTTACAAAAAGTGCTTTCCGAACTGAACAAAAAAGAGTAAAGTTAGTCGCGTAGGGTACAGAGGTAAGtagactggccccctgaatctccagacaaccaatatcacctaaataagtgatagtcttaacactcgttttt                                                                      <  2:722266‑M2/143‑71 (MQ=255)
                                                                                 ggcagcgggcttgtaatgggttaagtgataacagatgtctggaaatataggggcaaatccaGTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATGAc                                                               <  1:479913‑M1/82‑1 (MQ=255)
                                                                                   cagcgggcttgtaatgggttaagtgataacagatgtctggaaatataggggcaaatccaGTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTGATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATGACTg                                                             >  1:105746‑M1/60‑143 (MQ=255)
                                                                                    agggggcttgtaatgggttaggtgagagcagatgtctggaaatataggggcaaatccaGTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATGACTgg                                                            >  2:643751‑M1/59‑143 (MQ=255)
                                                                                    agcgggcttgtaatgggttaagtgataacagatgtctggaaatataggggcaaatccaGTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATGACTgg                                                            >  1:378837‑M1/59‑143 (MQ=255)
                                                                                      cgggcttgtaatgggttaagtgataacagatgtctggaaatataggggcaaatccaGTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAGTCGGATCTGGCTGATTATTTTAGCCGCCCCAGTCAGTAATGACTgggg                                                          >  1:31804‑M1/57‑143 (MQ=255)
                                                                                          cttgtaatgtgttaagtgataacagatgtctgtaaatatagggtcaaagccaGTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTTGCTGAATATTTTAGCCGCCCCAGTCAGTAATGACTGGGGCGtt                                                      >  2:353511‑M1/53‑143 (MQ=255)
                                                                                               aatgggttacgtgataacagatggccggaaatataggggcaaatccaGTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATGACTGGGGCGTTTTTTa                                                 >  1:86710‑M1/48‑143 (MQ=255)
                                                                                                     ctgaacAAAAAAGAGTAAAGTTAGTCGCGTAGGGTACAGAGGTAAGtagactggccccctgaatctccagacaaccaatatcacttaaataagtgatagtcttaatactagtttttagactagtcattggagaacagatgatt                                           >  1:259381‑M2/1‑46 (MQ=255)
                                                                                                       gaacAAAAAAGAGTAAAGTTAGTCGCGTAGGGTACAGAGGTAAGtagactggccccctgaatctccagacaaccaatatcacttaaataagtgatagtcttaatactagtctttagactagtcattggcgaacagatgattga                                         <  2:425245‑M2/143‑100 (MQ=255)
                                                                                                            ataacagatgtctggaaatataggggcaaatccaGTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATGACTGGGGCGTTTTTTATTGGGCgaaagaa                                    >  1:664704‑M1/35‑143 (MQ=255)
                                                                                                            aaaaaGAGGAAAGTTAGGCGCGTAGGGTACAGAGGTAAGtagactggccccctgaatctccagacaaccaatatcacttaaataagtgatagtcttaatactagtttttagactagtcattggagaacagatgattgatgtct                                    >  1:634382‑M2/1‑39 (MQ=255)
                                                                                                              aacagatgtctggaaatataggggcaaatccaGTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATGACTGGGGCGTTTTTTATTGGGCGaaagaaaa                                  <  2:165004‑M1/111‑1 (MQ=255)
                                                                                                               acagatgtctggaaatataggggcaaatccaGTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATGACTGGGGCGTTTTTTATTGGGCGaaagaaaag                                 <  1:53865‑M1/112‑1 (MQ=255)
                                                                                                               aaGAGTCAAGTTAGTCGCGTAGGGTACAGAGGTAAGtagactggccccctgaatctccagacaaccaatatcacttaaataagtgatagtcttaatactagtttttagactagtcattggagaacagatgattgatgtcttag                                 >  2:494917‑M2/1‑36 (MQ=255)
                                                                                                                cagatgtctggaaatataggggcaaatccaGTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATGACTGGGGCGTTTTTTATTGGGCGaaagaaaaga                                <  1:377101‑M1/113‑1 (MQ=255)
                                                                                                                cagatgtctggaaatataggggcaaatccaGTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATGACTGGGGCGTTTTTTATTGGGCGaaagaaaaga                                <  1:598682‑M1/113‑1 (MQ=255)
                                                                                                                  agTAAAGTTAGTCGCGTAGGGTACAGAGGTAAGtagactggccccctgaatctccagacaaccaatatcacttaaataagtgatagtcttaatactagtttttagactagtcatcggagaacagatgattgatgtgttagggc                              <  2:428295‑M2/143‑111 (MQ=255)
                                                                                                                   gTAAAGTTAGTCGCGTAGGGTACAGAGGTAAGtagactggccccctgaatctccagacaaccaatatcacttaaataagtgatagtcttaatactagtttttagactagtcattggagaacagatgattgatgtcttagggcc                             <  1:25786‑M2/143‑112 (MQ=255)
                                                                                                                    tgtctgtaaatataggagtaattccaGGAAGATGTTATATCTTTCAGACTTTTTACTTCACTTAATCGGATTTGGCGGAATATTTTAGCCGTGCCAGTCAGTAATGACTGGGGCGTTTTTTATTGGGAGAAATAAAAGATCCg                            >  2:536830‑M1/27‑143 (MQ=255)
                                                                                                                    tAAAGTTAGTCGCGTAGGGTACAGAGGTAAGtagactggccccctgaatctccagacaaccaatatcacttaaataagtgatagtcttaatactagtttttagactagtcattggagaacagatgattgatgtcttagggccg                            <  1:634794‑M2/143‑113 (MQ=255)
                                                                                                                     gtctggaaatataggggcaaatccaGTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATGACTGGGGCGGTTTTTATTgggagaaagaatagactcgt                           <  2:652338‑M1/118‑6 (MQ=255)
                                                                                                                       aGTTAGTCGCGTAGGGTACAGAGGTAAGtagactggccccctgaatctccagacaaccaatatcacttaaataagtgatagtcttaatactagtttttagactagtcattggagaacagatgattgatgtcttagggccggag                         >  1:350479‑M2/1‑28 (MQ=255)
                                                                                                                        tggaaatataggggcaaatccaGTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATGACTGGGGCGTTTTTTATTGGGGGAAAGAAAAGATACGtaag                        <  2:332337‑M1/121‑2 (MQ=255)
                                                                                                                          tAGTCGCGGAGGGTACAGAGGTAAGtagactggccccctgaatctccagacaatcaatatcacttagataagtgatagtcttaatactagtttttagactagtcattggagaacagatgattgatgtcttagggccggagaaa                      >  2:333297‑M2/1‑25 (MQ=255)
                                                                                                                          gaaatataggggcaaatccaGTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATGACTGGGGCGTTTTTTATTGGGCGAAAGAAAAGATCCGTAATGc                      <  1:172594‑M1/123‑1 (MQ=255)
                                                                                                                           aGTCGCGTAGGGTACAGAGGTAAGtagactggccccctgaatctccagacaaccaatatcacttaaataagtgatagtcttaatactagtttttagactagtcattggagaacagatgattgatgtcttagggccggagaaac                     >  1:391757‑M2/1‑24 (MQ=255)
                                                                                                                           aGTCGCGTAGGGTACAGAGGTAAGtagactggccccctgaatctccagacaaccaatatcacttaaataagtgatagtcttaatactagtttttagactagtcattggagaacagatgattgatgtcttagggccggagaaac                     <  2:86020‑M2/143‑120 (MQ=255)
                                                                                                                             ttataggggcatatccaGTATGATGTTCTATCTTTCAGTCCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATGACTGGGGCGTTTTTTATTGGGCGAAAGAAAAGATCCGTAATGCCTg                   >  2:224189‑M1/18‑143 (MQ=255)
                                                                                                                              tataggggcaaatccaGTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATGACTGGGGCGTTTTTTATTGGGCGAAAGAAAAGATCCGTACTGCCTGa                  <  1:437211‑M1/127‑1 (MQ=255)
                                                                                                                                taggggcaaatccaGTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATGACTGGGGCGTTTTTTATTGGGCGAAAGACAAGATCCGTAATGCCTGATg                <  1:451868‑M1/129‑1 (MQ=255)
                                                                                                                                       tACAGAGGTAAGtagactggccccctgaatctccagacaaccaatatcacttaaataagtgatagtcttaatactagtttttagactagtcattggagaacagatgattgatgtcttagggccggaggaacgcagacgccgga         <  2:170877‑M2/143‑132 (MQ=255)
                                                                                                                                         atccaGTAAGATGTTCTATCTTTCAGACCTTTTACTCCACGTAATCGGATGTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATGACTGGGGCGCTTTTTATTGGGCGAAAGAAAAGATCCGTAATGCCTGAtgcgctctggt       <  1:470403‑M1/138‑6 (MQ=255)
                                                                                                                                          agagGTAAGtagactggccccctgaatctccagacaacaaatctcacttcaataagtgatagtcttaatactagtctttagactagtcattggagaacagatgactgatgtcttagggccggagaaacgcagacggcgtacca      >  1:399428‑M2/1‑9 (MQ=255)
                                                                                                                                              gTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATGACTGGGGCGTTTTTTATTGGGCGAAAGAAAAGATCCGTAATGCCTGATGCGCTATGTTTATCa  <  2:549547/143‑1 (MQ=255)
                                                                                                                                              |                                                                                                                                              
CTTTTTTCGTAAGTAAGCAACATAAGCTGTCACGTTTTGTGATGGCTATTAGAAATTCCTATGCAACAACTGAAAAAAAATTACAAAAAGTGCTTTCTGAACTGAACAAAAAAGAGTAAAGTTAGTCGCGTAGGGTACAGAGGTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATGACTGGGGCGTTTTTTATTGGGCGAAAGAAAAGATCCGTAATGCCTGATGCGCTATGTTTATCA  >  NC_000913/4049770‑4050054

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.