Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC JC	NC_000913	4,049,912	IS2 (+) +5 bp	noncoding (14‑18/109 nt)	spf →	Spot 42 sRNA antisense regulator of galK translation, Hfq‑dependent

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	3186096 =	NA (NA)	40 (0.990)	35/284	0.2	100%	noncoding (1/1331 nt)	IS2	repeat region
	?	NC_000913	= 4049916	0 (0.000)					noncoding (18/109 nt)	spf	Spot 42 sRNA antisense regulator of galK translation, Hfq‑dependent
*	?	NC_000913	4049912 =	0 (0.000)	42 (1.040)	36/284	0.1	100%	noncoding (14/109 nt)	spf	Spot 42 sRNA antisense regulator of galK translation, Hfq‑dependent
	?	NC_000913	= 4499511	NA (NA)					noncoding (1331/1331 nt)	IS2	repeat region

CTTGCAGAGGCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTACAAGCGACTGTATTACAGTCTGCCATATATGATTACCTGTGATCAGGAAGAATAAGTGGCTGAGGTTTCAAACTGTCTGCCGGTATATTCCT  >  NC_000913/4499372‑4499652                                                                                                                                            |                                                                                                                                              cTTGCAGAGGCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATTTCTGCGGCAGCGGGCTTGTAATAGGTTAAGTAAAAAAACATGACAGAAAATAAAGGGACAAATCCACCt                                                                                                                                            >  1:256275/1‑143 (MQ=11) cTTGCAGAGGCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCt                                                                                                                                            >  1:683385/1‑143 (MQ=31) cTTGCAGAGGCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCCCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGGTAAGTGATAACAGATGTCTGGAAGTGTAAGGGCAAATCCAccg                                                                                                                                            >  2:175864/1‑142 (MQ=11)            gTTCGAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGatat                                                                                                                                 >  1:561008/1‑143 (MQ=40)                gAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAAt                                                                                                                             <  1:571411/143‑1 (MQ=255)                 aGCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATa                                                                                                                            >  1:437639/1‑143 (MQ=255)                  gCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATAc                                                                                                                           >  1:203857/1‑143 (MQ=255)                        taACGAATGGCATCGGCATGGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGAGGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGatat                                                                                                                     >  2:15840/1‑143 (MQ=255)                        taACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTGGTAATGGGGTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGatat                                                                                                                     >  1:506741/1‑143 (MQ=255)                         aaCGAATGGCATCCGCATAGTGCGCTGGGGTATCGCTCGCCACGGGAATCGCTGCGGCAGCGGGCTTGTAGTGGGTTCAGTGATAACAGATGTCTGGGAATATAGGGGCAAATCGCCCTGGATCTGATATGAATACGGATAtt                                                                                                                    >  2:346204/1‑143 (MQ=14)                                     ccGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATGTTTTATACAGAATa                                                                                                        >  2:722240/1‑143 (MQ=255)                                      cGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCGGGAAATATAGGGGCAAGTCCACCTTGTGCTGATATGAACACGGATATTTTATACAGAAGAc                                                                                                       >  1:589796/1‑143 (MQ=255)                                       gCATAGTGCGCTGGGTTATCGCTAGCATCATTAATATCTGCGGCAGCGAGCTTGTAATGGGTTAAGTGATAACAGATGTCCGGACATACAGGGGAAAATCCACCTTGTGCTGATATGAATACGGAGATTGTATAAAGAGTACt                                                                                                      >  2:89438/1‑143 (MQ=17)                                           aGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTc                                                                                                  >  1:317081/1‑143 (MQ=255)                                            gTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCa                                                                                                 <  2:8514/143‑1 (MQ=255)                                            gTGCGCTGGGTTATCGCTCGACACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCa                                                                                                 <  1:553229/143‑1 (MQ=255)                                            gTGCGCTGGGTAATCGCTCGCCACAGGAATATGTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCa                                                                                                 <  1:433342/143‑1 (MQ=255)                                             tGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTAAg                                                                                                >  1:57562/1‑143 (MQ=255)                                               cgcTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGGTAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTa                                                                                              >  1:381989/1‑143 (MQ=255)                                               cgcTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCATGTGCTGATATGAATACGGATATTTTATACAGAATACCTTTCAGTa                                                                                              >  2:217466/1‑143 (MQ=255)                                                  tGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTACaa                                                                                           >  1:384383/1‑143 (MQ=255)                                                   gggTTATCGCTGGCCACGGGATTTTCTGGGGTAGCGGGCTTGTTGTGGGTTGGGTGTTGTCGGCTGTGTGGTAATATAGGGGCAAATCCACCTTGTGCTGATCTGACTACGGATATTTTATACAGAATACTTTTCAGTACAAg                                                                                          <  2:13783/143‑1 (MQ=255)                                                      ttATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTACAAGCGa                                                                                       <  2:230829/143‑1 (MQ=255)                                                      ttATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTACAAGCGa                                                                                       >  1:436348/1‑143 (MQ=255)                                                       tATGGCTCGGCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGGCTGGAAAGATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTACAAGCGAc                                                                                      <  2:566704/143‑1 (MQ=255)                                                          cgctcgcCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGCATAAGTGATAACAGATGTCTGGAAATATAAGGGCAAATCAACCATATGCTGATAGGAATACGGATATTAAATACAGAATACTATACAGTACAAGCAACTGt                                                                                   >  2:353780/1‑143 (MQ=18)                                                                ccACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTACAAGCGACTGTATTACa                                                                             >  1:618160/1‑143 (MQ=255)                                                                ccACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTACAAGCGACTGTATTACa                                                                             >  1:656851/1‑143 (MQ=255)                                                                       aaTATTTGCGGCAGTGTGCGTTTAATGGGTTAAGTGATAACAGATGTCTGGAAGTATAGAGGCAAATCTACCTTTTGCTGATATGAATTCGGATATTTTATTTAGAATAATTTTCACTACAAGCGACTGTATTACAGTCTGcc                                                                      <  2:373594/143‑1 (MQ=21)                                                                                 gCAGCGGGCCTGTAATGGGTGAAGTGATAACAGATGTATGGGAATATAGGGGCAAATACACCTTGGGCTGATATGAATACGGATATGTTAGACAGAATACTTTTCAGTACAAGCGACTGTATTACAGTCTGCCATATATGAtt                                                            <  1:651329/143‑1 (MQ=255)                                                                                  cAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTCTTCAGTACAAGCGACTGTAGTACAGTCTGCCATATATGATTa                                                           >  1:265344/1‑143 (MQ=255)                                                                                      gggCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCGACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTACAAGCGACGGCATTACCGTTTGCCATATATTATTACCtg                                                       >  1:719521/1‑143 (MQ=37)                                                                                            gTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTACAAGCGACTGTATTACAGTCTGCCATATATGATTACCTGTGATCa                                                 <  1:669015/143‑1 (MQ=255)                                                                                            gTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTACAAGCGACTGTATTACAGTCTGCCATATATGATTACCTGTGATCa                                                 <  2:203857/143‑1 (MQ=255)                                                                                            gTAATGGGGTAAGTGAGAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTACAAGCGACTGTATTACAGTCTGCCATATATGATTACCTGTGATCa                                                 <  2:34534/143‑1 (MQ=255)                                                                                                  ggTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGTATATTTTATACAGAATACTTTTCAGTACAAGCGACTGTATTACAGTCTGCCATATATGATTACCTGTGATCAGgaaga                                           <  2:437639/143‑1 (MQ=255)                                                                                                       aGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTACAAGCGACTGTATTACAGTCTGCCATATATGATTACCTGTGATCAGGAAGAATAAg                                      <  1:502543/143‑1 (MQ=255)                                                                                                        gTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTACAAGCGACTGTATTACAGTCTGCCATATATGATTACATGTGATCAGGAAGAATAAGt                                     >  2:172400/1‑143 (MQ=255)                                                                                                         tGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTACAAGCGACTGTATTACAGTCTGCCATATATGATTACCTGTGCTCAGGCAGAATAAGTg                                    >  2:312080/1‑143 (MQ=255)                                                                                                                       tGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTACAAGCGACTGTATTACAGTCTGCCATATATGATTACCTGTGATCAGGAAGAATAAGTGGCTGAgggtgcaga                      >  1:187275/1‑136 (MQ=255)                                                                                                                       tGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTACAAGCGACTGTATTACAGTCTGCCATATATGATTACCTGTGATCAGGAAGAATAAGTGGCTGAGGTTTCaaa                      >  1:312397/1‑143 (MQ=255)                                                                                                                       gggAAATATAGGGGCAAATCCACCGTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTACAAGCGACTGTATTACAGTCTGCCATATATGATTACCTGTGATCAGGAAGAATAAGTGGCTGAGGTTTCaaa                      <  1:219392/142‑1 (MQ=255)                                                                                                                             tataGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTACAAGCGACTGTATTACAGTCTGCCATATATGATTACCTGTGATCAGGAAGAATAAGTGGCTGAGGTTTCAAACTGTCt                >  1:249526/1‑143 (MQ=255)                                                                                                                              ataGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTAGAAGCGACTGGATTACAGTCTGCCATATATGATTACCTGTGATCAGGAAGAATAAGTGGCTGAGGTTTCAAACTGTCTg               <  2:219317/143‑1 (MQ=255)                                                                                                                                 agggCACATCCACCTTGCGCGGATATGAATACGGATATTTTATACAGAATACTTTTCAGTACAAGCGACTGTATTACAGTCTGCCATATATGATTACCTGTGATCAGGAAGAATAAGTGGCTGAGGTTTCAAACTGTCTGCCg            <  1:268257/142‑1 (MQ=255)                                                                                                                                  gggCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTACAAGCGACTGTATTACAGTCTGCCATATATGATTACCTGTGATCAGGAAGAATAAGTGGCTGAGGTTTCAAACTGTCTGCCgg           >  1:322317/1‑143 (MQ=255)                                                                                                                                           cACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTACAAGCGACTGTATTACAGTCTGCCATATATGATTACCTGTGATCAGGAAGAATAAGTGGCTGAGGTTTCAAACTGTCTGCCGGTATATTCct  <  2:618160/143‑1 (MQ=255)                                                                                                                                            |                                                                                                                                              CTTGCAGAGGCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTACAAGCGACTGTATTACAGTCTGCCATATATGATTACCTGTGATCAGGAAGAATAAGTGGCTGAGGTTTCAAACTGTCTGCCGGTATATTCCT  >  NC_000913/4499372‑4499652

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.