Predicted mutation
evidence seq id position mutation annotation gene description
JC JC NC_000913 4,049,912 IS2 (+) +5 bp noncoding (14‑18/109 nt) spf → Spot 42 sRNA antisense regulator of galK translation, Hfq‑dependent

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 3186096 =NA (NA)40 (0.990) 35/284 0.2 100% noncoding (1/1331 nt) IS2 repeat region
?NC_000913 = 4049916 0 (0.000)noncoding (18/109 nt) spf Spot 42 sRNA antisense regulator of galK translation, Hfq‑dependent
* ? NC_000913 4049912 =0 (0.000)42 (1.040) 36/284 0.1 100% noncoding (14/109 nt) spf Spot 42 sRNA antisense regulator of galK translation, Hfq‑dependent
?NC_000913 = 4499511 NA (NA)noncoding (1331/1331 nt) IS2 repeat region

CTTGCAGAGGCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTACAAGCGACTGTATTACAGTCTGCCATATATGATTACCTGTGATCAGGAAGAATAAGTGGCTGAGGTTTCAAACTGTCTGCCGGTATATTCCT  >  NC_000913/4499372‑4499652
                                                                                                                                           |                                                                                                                                             
cTTGCAGAGGCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATTTCTGCGGCAGCGGGCTTGTAATAGGTTAAGTAAAAAAACATGACAGAAAATAAAGGGACAAATCCACCt                                                                                                                                            >  1:256275/1‑143 (MQ=11)
cTTGCAGAGGCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCt                                                                                                                                            >  1:683385/1‑143 (MQ=31)
cTTGCAGAGGCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCCCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGGTAAGTGATAACAGATGTCTGGAAGTGTAAGGGCAAATCCAccg                                                                                                                                            >  2:175864/1‑142 (MQ=11)
           gTTCGAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGatat                                                                                                                                 >  1:561008/1‑143 (MQ=40)
               gAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAAt                                                                                                                             <  1:571411/143‑1 (MQ=255)
                aGCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATa                                                                                                                            >  1:437639/1‑143 (MQ=255)
                 gCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATAc                                                                                                                           >  1:203857/1‑143 (MQ=255)
                       taACGAATGGCATCGGCATGGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGAGGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGatat                                                                                                                     >  2:15840/1‑143 (MQ=255)
                       taACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTGGTAATGGGGTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGatat                                                                                                                     >  1:506741/1‑143 (MQ=255)
                        aaCGAATGGCATCCGCATAGTGCGCTGGGGTATCGCTCGCCACGGGAATCGCTGCGGCAGCGGGCTTGTAGTGGGTTCAGTGATAACAGATGTCTGGGAATATAGGGGCAAATCGCCCTGGATCTGATATGAATACGGATAtt                                                                                                                    >  2:346204/1‑143 (MQ=14)
                                    ccGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATGTTTTATACAGAATa                                                                                                        >  2:722240/1‑143 (MQ=255)
                                     cGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCGGGAAATATAGGGGCAAGTCCACCTTGTGCTGATATGAACACGGATATTTTATACAGAAGAc                                                                                                       >  1:589796/1‑143 (MQ=255)
                                      gCATAGTGCGCTGGGTTATCGCTAGCATCATTAATATCTGCGGCAGCGAGCTTGTAATGGGTTAAGTGATAACAGATGTCCGGACATACAGGGGAAAATCCACCTTGTGCTGATATGAATACGGAGATTGTATAAAGAGTACt                                                                                                      >  2:89438/1‑143 (MQ=17)
                                          aGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTc                                                                                                  >  1:317081/1‑143 (MQ=255)
                                           gTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCa                                                                                                 <  2:8514/143‑1 (MQ=255)
                                           gTGCGCTGGGTTATCGCTCGACACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCa                                                                                                 <  1:553229/143‑1 (MQ=255)
                                           gTGCGCTGGGTAATCGCTCGCCACAGGAATATGTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCa                                                                                                 <  1:433342/143‑1 (MQ=255)
                                            tGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTAAg                                                                                                >  1:57562/1‑143 (MQ=255)
                                              cgcTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGGTAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTa                                                                                              >  1:381989/1‑143 (MQ=255)
                                              cgcTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCATGTGCTGATATGAATACGGATATTTTATACAGAATACCTTTCAGTa                                                                                              >  2:217466/1‑143 (MQ=255)
                                                 tGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTACaa                                                                                           >  1:384383/1‑143 (MQ=255)
                                                  gggTTATCGCTGGCCACGGGATTTTCTGGGGTAGCGGGCTTGTTGTGGGTTGGGTGTTGTCGGCTGTGTGGTAATATAGGGGCAAATCCACCTTGTGCTGATCTGACTACGGATATTTTATACAGAATACTTTTCAGTACAAg                                                                                          <  2:13783/143‑1 (MQ=255)
                                                     ttATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTACAAGCGa                                                                                       <  2:230829/143‑1 (MQ=255)
                                                     ttATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTACAAGCGa                                                                                       >  1:436348/1‑143 (MQ=255)
                                                      tATGGCTCGGCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGGCTGGAAAGATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTACAAGCGAc                                                                                      <  2:566704/143‑1 (MQ=255)
                                                         cgctcgcCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGCATAAGTGATAACAGATGTCTGGAAATATAAGGGCAAATCAACCATATGCTGATAGGAATACGGATATTAAATACAGAATACTATACAGTACAAGCAACTGt                                                                                   >  2:353780/1‑143 (MQ=18)
                                                               ccACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTACAAGCGACTGTATTACa                                                                             >  1:618160/1‑143 (MQ=255)
                                                               ccACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTACAAGCGACTGTATTACa                                                                             >  1:656851/1‑143 (MQ=255)
                                                                      aaTATTTGCGGCAGTGTGCGTTTAATGGGTTAAGTGATAACAGATGTCTGGAAGTATAGAGGCAAATCTACCTTTTGCTGATATGAATTCGGATATTTTATTTAGAATAATTTTCACTACAAGCGACTGTATTACAGTCTGcc                                                                      <  2:373594/143‑1 (MQ=21)
                                                                                gCAGCGGGCCTGTAATGGGTGAAGTGATAACAGATGTATGGGAATATAGGGGCAAATACACCTTGGGCTGATATGAATACGGATATGTTAGACAGAATACTTTTCAGTACAAGCGACTGTATTACAGTCTGCCATATATGAtt                                                            <  1:651329/143‑1 (MQ=255)
                                                                                 cAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTCTTCAGTACAAGCGACTGTAGTACAGTCTGCCATATATGATTa                                                           >  1:265344/1‑143 (MQ=255)
                                                                                     gggCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCGACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTACAAGCGACGGCATTACCGTTTGCCATATATTATTACCtg                                                       >  1:719521/1‑143 (MQ=37)
                                                                                           gTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTACAAGCGACTGTATTACAGTCTGCCATATATGATTACCTGTGATCa                                                 <  1:669015/143‑1 (MQ=255)
                                                                                           gTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTACAAGCGACTGTATTACAGTCTGCCATATATGATTACCTGTGATCa                                                 <  2:203857/143‑1 (MQ=255)
                                                                                           gTAATGGGGTAAGTGAGAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTACAAGCGACTGTATTACAGTCTGCCATATATGATTACCTGTGATCa                                                 <  2:34534/143‑1 (MQ=255)
                                                                                                 ggTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGTATATTTTATACAGAATACTTTTCAGTACAAGCGACTGTATTACAGTCTGCCATATATGATTACCTGTGATCAGgaaga                                           <  2:437639/143‑1 (MQ=255)
                                                                                                      aGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTACAAGCGACTGTATTACAGTCTGCCATATATGATTACCTGTGATCAGGAAGAATAAg                                      <  1:502543/143‑1 (MQ=255)
                                                                                                       gTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTACAAGCGACTGTATTACAGTCTGCCATATATGATTACATGTGATCAGGAAGAATAAGt                                     >  2:172400/1‑143 (MQ=255)
                                                                                                        tGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTACAAGCGACTGTATTACAGTCTGCCATATATGATTACCTGTGCTCAGGCAGAATAAGTg                                    >  2:312080/1‑143 (MQ=255)
                                                                                                                      tGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTACAAGCGACTGTATTACAGTCTGCCATATATGATTACCTGTGATCAGGAAGAATAAGTGGCTGAgggtgcaga                      >  1:187275/1‑136 (MQ=255)
                                                                                                                      tGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTACAAGCGACTGTATTACAGTCTGCCATATATGATTACCTGTGATCAGGAAGAATAAGTGGCTGAGGTTTCaaa                      >  1:312397/1‑143 (MQ=255)
                                                                                                                      gggAAATATAGGGGCAAATCCACCGTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTACAAGCGACTGTATTACAGTCTGCCATATATGATTACCTGTGATCAGGAAGAATAAGTGGCTGAGGTTTCaaa                      <  1:219392/142‑1 (MQ=255)
                                                                                                                            tataGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTACAAGCGACTGTATTACAGTCTGCCATATATGATTACCTGTGATCAGGAAGAATAAGTGGCTGAGGTTTCAAACTGTCt                >  1:249526/1‑143 (MQ=255)
                                                                                                                             ataGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTAGAAGCGACTGGATTACAGTCTGCCATATATGATTACCTGTGATCAGGAAGAATAAGTGGCTGAGGTTTCAAACTGTCTg               <  2:219317/143‑1 (MQ=255)
                                                                                                                                agggCACATCCACCTTGCGCGGATATGAATACGGATATTTTATACAGAATACTTTTCAGTACAAGCGACTGTATTACAGTCTGCCATATATGATTACCTGTGATCAGGAAGAATAAGTGGCTGAGGTTTCAAACTGTCTGCCg            <  1:268257/142‑1 (MQ=255)
                                                                                                                                 gggCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTACAAGCGACTGTATTACAGTCTGCCATATATGATTACCTGTGATCAGGAAGAATAAGTGGCTGAGGTTTCAAACTGTCTGCCgg           >  1:322317/1‑143 (MQ=255)
                                                                                                                                          cACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTACAAGCGACTGTATTACAGTCTGCCATATATGATTACCTGTGATCAGGAAGAATAAGTGGCTGAGGTTTCAAACTGTCTGCCGGTATATTCct  <  2:618160/143‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                             
CTTGCAGAGGCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTACAAGCGACTGTATTACAGTCTGCCATATATGATTACCTGTGATCAGGAAGAATAAGTGGCTGAGGTTTCAAACTGTCTGCCGGTATATTCCT  >  NC_000913/4499372‑4499652

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.