breseq  version 0.32.0  revision 6ff6de7d1b87
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation freq annotation gene description
MC JC 257,908 Δ776 bp 100% [crl] [crl]
MC JC 1,299,499 Δ1,199 bp 100% intergenic (+254/‑485) ychE → / → oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein
MC JC 1,978,503 Δ776 bp 100% insB1insA insB1, insA
RA 2,132,787 A→C 100% I204S (ATC→AGC)  wcaA ← putative glycosyl transferase
RA 2,590,667 G→T 8.1% L1025L (CTG→CTT acrD → aminoglycoside/multidrug efflux system
RA 3,469,602 A→G 5.8% F84L (TTT→CTT)  chiA ← periplasmic endochitinase
RA 3,666,236 G→A 5.1% L448L (CTC→CTT gadA ← glutamate decarboxylase A, PLP‑dependent
RA 3,730,008 A→G 8.8% F253L (TTC→CTC)  xylA ← D‑xylose isomerase
MC JC 3,815,858 Δ82 bp 100% [rph][rph] [rph], [rph]
MC JC 4,001,644 Δ5 bp 100% coding (220‑224/951 nt) corA → magnesium/nickel/cobalt transporter
RA 4,184,542 C→A 100% P1100Q (CCG→CAG)  rpoB → RNA polymerase, beta subunit
RA 4,296,189 A→G 43.5% intergenic (+396/+248) gltP → / ← yjcO glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein
RA 4,296,190 A→C 38.2% intergenic (+397/+247) gltP → / ← yjcO glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein
RA 4,296,267 T→C 19.1% intergenic (+474/+170) gltP → / ← yjcO glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein
RA 4,512,561:1 +G 6.3% coding (850/999 nt) fecC ← ferric citrate ABC transporter permease
RA 4,532,766 Δ1 bp 11.1% coding (419/747 nt) yjhP ← putative methyltransferase

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 300090 300111 22 5 [4] [4] 5 paoC PaoABC aldehyde oxidoreductase, Moco‑containing subunit
* * ÷ NC_000913 720946 721198 253 5 [4] [4] 5 [kdpE] [kdpE]
* * ÷ NC_000913 1212022 1212126 105 5 [2] [4] 5 iraM/ycgX RpoS stabilzer during Mg starvation, anti‑RssB factor/DUF1398 family protein
* * ÷ NC_000913 2033792 2033973 182 5 [3] [3] 5 rseX/yedS sRNA antisense regulator of ompA and ompC translation, Hfq‑dependent/pseudogene, outer membrane protein homology, putative outer membrane protein
* * ÷ NC_000913 3423898–3424231 3424384–3424236 6–487 5 [4] [4] 6 rrlD 23S ribosomal RNA of rrnD operon
* * ÷ NC_000913 3799089 3799233 145 5 [4] [4] 5 waaU lipopolysaccharide core biosynthesis
* * ÷ NC_000913 4233787 4235376 1590 5 [0] [0] 5 pgi glucosephosphate isomerase

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 257908 =NA (NA)26 (0.670) 23/476 NT 100% intergenic (+9/‑768) crl/crl pseudogene, sigma factor‑binding protein, RNA polymerase holoenzyme formation stimulator,regulator, Surface structures, transcriptional regulator of cryptic csgA gene for curli surface fibers/pseudogene, sigma factor‑binding protein, RNA polymerase holoenzyme formation stimulator,regulator, Surface structures, transcriptional regulator of cryptic csgA gene for curli surface fibers
?NC_000913 = 1293039 0 (0.000)intergenic (‑117/‑488) hns/tdk global DNA‑binding transcriptional dual regulator H‑NS/thymidine kinase/deoxyuridine kinase
* ? NC_000913 1293032 =0 (0.000)35 (0.900) 28/476 NT 100% intergenic (‑110/‑495) hns/tdk global DNA‑binding transcriptional dual regulator H‑NS/thymidine kinase/deoxyuridine kinase
?NC_000913 = 1979270 NA (NA)intergenic (‑55/‑483) insA/uspC IS1 repressor TnpA/universal stress protein
* ? NC_000913 1979486 =0 (0.000)19 (0.490) 18/476 NT 100% intergenic (‑271/‑267) insA/uspC IS1 repressor TnpA/universal stress protein
?NC_000913 2101749 = NA (NA)intergenic (‑5/+146) wbbL/insH1 pseudogene, lipopolysaccharide biosynthesis protein/IS5 transposase and trans‑activator
* ? NC_000913 = 19794890 (0.000)24 (0.620) 23/476 NT 100% intergenic (‑274/‑264) insA/uspC IS1 repressor TnpA/universal stress protein
?NC_000913 = 2290111 NA (NA)intergenic (‑32/+1) insH1/yejO IS5 transposase and trans‑activator/pseudogene, autotransporter outer membrane homology,putative transport, Not classified, putative ATP‑binding component of a transport system