Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC JC	NC_000913	2,536,312	Δ1 bp :: IS186 (+) +6 bp :: Δ1 bp	coding (479‑484/510 nt)	crr →	glucose‑specific enzyme IIA component of PTS

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	2514274 =	NA (NA)	47 (0.790)	41/278	0.4	100%	noncoding (2/1345 nt)	IS186	repeat region
	?	NC_000913	= 2536317	0 (0.000)					coding (484/510 nt)	crr	glucose‑specific enzyme IIA component of PTS
*	?	NC_000913	= 2515616	NA (NA)	64 (1.060)	47/280	0.2	100%	noncoding (1344/1345 nt)	IS186	repeat region
	?	NC_000913	2536312 =	0 (0.000)					coding (479/510 nt)	crr	glucose‑specific enzyme IIA component of PTS

AATGAAGAGCAAGGTAACGTGGTTTCTCGCTTCGGTCTGAAGCTGGTTTACGGCTCTACCCTGGTGAGTGTGCTGTCTGCGTCAATCGCAGCACTGGTGCTGTAAGACCATACATAAAAAAGCCGGGGATAATTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTGGCCCATATTGGTAAGCCCGAAGAACTGGATACTTCGGCACGTAATGCC  >  NC_000913/2514140‑2514414                                                                                                                                       |                                                                                                                                             gatgaaGAGCGTGGTAACGTGGTTGCTCGTTTCGGTCTGAAGCTGGTTTACGGCTCTACCCTGGTGAGTGTGCTGTCTGCGTCAATCGCAGCACTGGTGCTGTAAGACCATACATAAAAAAGCCGGGGATAATTCCCATAAgc                                                                                                                                      <  2:879119/142‑1 (MQ=255)  aggaaGAGCAAGGTAACGTGGTTTCTCGCTTCGGTCTGAAGCTGGTTTACGGCTCTACCCTGGTGAGTGTGCTGTCTGCGTCAATCGCAGCACTGGTGCTGTAAGACCATACATAAAAAAGCCGGGGATAATTCCCATAAgcg                                                                                                                                     <  2:1094873/141‑1 (MQ=255)     aaGAGCAAGGTAACGTGGTTTCTCGCTTCGGTCTGAAGCTGGTTTACGGCTCTACCCTGGTGAGTGTGCTGTCTGCGTCAATCGCAGCACTGGTGCTGTAAGACCATACATAAAAAAGCCGGGGATCATTCCCACCAGCGCTa                                                                                                                                  >  2:1053816/1‑143 (MQ=255)      agagCAAGGTAACGTGGTTTCTCGCTTCGGTCTGAAGCTGGTTTACGGCTCTACCCTGGTGAGTGTGCTGTGTGCGGCAATCGCAGCACTGGTGCTGTAAGACCATACATAAAAAAGCCGGGGGTAATTCCCATAAGCGCTaa                                                                                                                                 >  1:59665/1‑143 (MQ=255)      agagCAAGGTAACGTGGTTGCTCGCTTCGGTCTGAAGCTGGTTTACGGCTCTACCCTGGTGAGTGTGCTGTCTGCGTCAATCGCAGCACTGGTGCTGTAAGACCATACATAAAAAAGCCGGGGATAATTCCCATAAGCGCTaa                                                                                                                                 <  1:275082/143‑1 (MQ=255)             ggTCACGTGGTTGCTCGCTGCGGTCGGAGGCTGGTTTACGGCTCTACCCGGGTGAGTGTGCTGTCTGCGTCAATCGCAGCACTGGTGCTGTAAGACCATACATAAAAAAGCCGGGGATAATTCCCATAAGCGCTAACTTAAgg                                                                                                                          <  2:214296/143‑1 (MQ=255)                   gTGGTTTCTCGCTTCGGTCTGAAGCTGGTTTACGGCTCTACCCTGGTGAGTGTGCTGTCTGCGTCAATCGCAGCACTGGTGCTGTAATACCATACATAAAACAGCCGGGGATAATTCCCCTCCGCCCTAACTTTTCGGTtgtg                                                                                                                    >  1:1069898/1‑143 (MQ=255)                   gTGGTTTCTCGCTTCGGTCTGAAGCTGGTTTACGGCTCTACCCTGGTGAGTGTGCTGTCTGCGTCAATCGCAGCACTGGTGCTGTAAGACCATACATAACAAAGCCGGGGATAATTCCCATAAGCGCTCACTTAAGGGTtgtg                                                                                                                    >  1:783728/1‑143 (MQ=255)                   gTGGTTTCTCGCTTCGGTATGACATTGTTGTACCGCGCTCCGCTGGTGAGTCTGCTGTCTGGGTCAATCGCAGCACTGGTGCTGTAATACCATACATAAAAAAGCCGGGGATAATTCCCATAAGCGCTAACTTAAGGGTtgtg                                                                                                                    <  2:527432/143‑1 (MQ=255)                        ttCTCGCTTCGGTCTGAAGCTGGTTTACGGCTCTACCCTGGTGAGAGTGCTGTCTGCGTCAATCGCAGCACTGGTGCTGTAAGACCATACATAAAAAAGCCGGGGATAATTCCCATAAGCGCTAACTTAAGGGTTGTGGTAtt                                                                                                               >  1:1060130/1‑143 (MQ=255)                                tCGGTCTGAAGCTGGTTTACGGCTCTACCCTGGTGAGTGTGCTGTCTGCGTCAATCGCAGCACTGGTGCTGTAAGACCATACATAAAAAAGCCGGGGATAATTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGa                                                                                                       <  1:647466/143‑1 (MQ=255)                                 cGGTCTGAAGCTGGTTTACGGCTCTGCCCTGGTGAGTGTGCTGTCTGCGTCAATCGCAGCACTGGTGCTGTAAGACCATACATAAAAAAGCCGGGGATAATTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGat                                                                                                      <  2:1064745/143‑1 (MQ=255)                                  ggTGTGAAGCTGGTGTACGGCTCTACCCTGGTGAGGGTGCTGTCTGCGTCAATCGCAGCACTGGTGCTGTAAGACCATACATAAAGAAGCCGGGGAGAATTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGata                                                                                                     <  2:64051/143‑1 (MQ=38)                                    tCTGAAGCTGGTTTACGGCTCTACCCTGGTGAGTGTGCTGTCTGCGTCAATCGCAGCACTGGTGCTGTAAGACCATACATAAAAAAGCCGGGGATAATTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATg                                                                                                   >  1:1078032/1‑143 (MQ=255)                                     cTGGAGCTGGTTTTCGGCTCTACCCTGGTGAGTGTGCTGTCTGCGTCAATCGCAGCACTGGTGCTGTAAGACCATACATCAAAAAGCCGGGGATAATTCCCATATGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGa                                                                                                  <  1:676748/143‑1 (MQ=255)                                     cTGAAGCTGGTTTACGGCTCTACCCTGGTGCGCCTAATTTCGCTGTCATTCGCAGCACTGTTGCTGTAAGACCATACATAAAAAAGCCGGGGATAATTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGa                                                                                                  <  2:487083/143‑1 (MQ=25)                                       gAAGCTGGATTACGGCTCTACCCTGGTGAGTGTGCTGTCTGCGTCAATCGCAGCACTGGTGCTGTAAGACCATACATAAAAAAGCCGGGGATAATCCCCAGAAGCGCTAACGTAAGGGTTGTGGTATTACGCCTGATATGAtt                                                                                                <  2:337968/143‑1 (MQ=255)                                                ttACGGCTCTACCCTGGTGAGTGTGCTGTCTGCGTCAATCGCAGCACTGGTGCTGTCAGACCACACATAAAAAAGCCGGGGATACTTCAAATAAGCGCTAACTTAAAGGTTGTGATATAAAGACTGATACGATATACCGTGcc                                                                                       >  2:588469/1‑143 (MQ=255)                                                         tACCCTGGTGAGTGTGCTGTCTGCGTCAATCGCAGCACTGGTGCTGTAAGACCATACATAAAAAAGCCGGGGATAATTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGAGATAACGTGCGGATGAATTa                                                                              >  2:564635/1‑143 (MQ=255)                                                           ccgtGGTGAGTGTGCTGTCTGCGTCAGTCGCAGAGCTGGCGCGAGATTACCATACATAAAAAAGCCGGGGATAATTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTAct                                                                            <  1:625108/140‑1 (MQ=18)                                                              tGGTGAGTGTGCTGTCTGCGTCAATCGCAGCACTGGTGCTGTAAGACCATACATAAAAAAGCCGGGGATAATTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACtctc                                                                         <  1:232328/143‑1 (MQ=255)                                                               ggTGAGTGTGCTGTCTGCGTCAATCGCAGCACTGGTGCTGTAAGACCATACATAAAAAAGCCGGGGATAATTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCa                                                                        <  1:644547/143‑1 (MQ=255)                                                               ggTGAGTGTGCTGTCTGCGTCAATCGCAGCACTGGTGCTGTAAGACCAGACATAAAAAAGCCGGGGATAATTCCCATAAGCGCTAACTCAAGGGTTGTGGTATTACGCCCGATATGATCTAACGTGCTGATGAATTCCCCTCa                                                                        >  2:849188/1‑143 (MQ=25)                                                                gTGAGTGTGCTGTCTGCGTCAATCGCAGCACTGGTGCTGTAAGACCATACATAAAAAAGCCGGGGATAATTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATTCATTACTCTAAc                                                                       >  1:1052287/1‑143 (MQ=255)                                                                     tgtgCTGTCTGCGTCAATCGCAGCACTGGTGCTGTAAGACCAGACATAAAAAAGCCGGGGATAATTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATaa                                                                  <  1:1029956/143‑1 (MQ=255)                                                                          tGTCTGCGTCAATCGCAGCACTGGTGCTGTAAGACCATACATAAAAAAGCCGGGGATAATTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGCTTTAACGTGCCGATGAATTACTCTCACGATAACTGGt                                                             >  2:994558/1‑143 (MQ=255)                                                                          tGTCTCCGTCCATCGCTGCACTGTTGCTGTAAGACCATACATAAAAAAGCCTGGTATCATCCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTTCTCTCACGATAACTGGt                                                             <  2:855419/143‑1 (MQ=18)                                                                                cGTCAATCGCAGCACTGGTGCTGTAAGACCATACATAAAAAAGCCGGGGATAATTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCaa                                                       >  2:314823/1‑143 (MQ=255)                                                                                 gTCAATCGCAGCACTGGTGCTGTAAGACCATACATAAAAAAGCCGGGGATAATTCCCATAAGCGCTAACTTACGGGTTGTGGTATTACGCCTGATATGATTTTACTTGCCGATGAATTGCTCTCGCGCTCACTGTTCAGCatt                                                      >  1:692388/1‑141 (MQ=21)                                                                                  tCAATCGCAGCACTGGTGCTGTAAGACCATACATAAAAAAGCCGGGGATAATTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAAtt                                                     <  2:1060130/143‑1 (MQ=255)                                                                                   caagcccagcaCTGGTGCTGTAAGACCATGCATAAAAAAGCCGGGGATAATTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGTATTACTCTCACGATAACTGGTCAGCAATTc                                                    <  1:364842/137‑1 (MQ=255)                                                                                   cAATCGCAGCACTGGTGCTGTAAGACCATACATAAAAAAGCCGGGGATAATTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAg                                                          <  2:99524/137‑1 (MQ=255)                                                                                   cAATCGCAGCACTGGTGCTGTAAGACCATACATAAAAAAGCCGGGGATAATTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAg                                                          >  1:99524/1‑137 (MQ=255)                                                                                   cAATCGCAGCACTGGTGCTGTAAGACCATACATAAAAAAGCCGGGGATAATTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTc                                                    <  1:290911/143‑1 (MQ=255)                                                                                     aTCGCAGCACTGGTGCTGTAAGACCATACATAAAAAAGCCGGGGATAATTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCGATTCTg                                                  >  1:584312/1‑143 (MQ=255)                                                                                     aTCGCAGCACTGGTGCTGTAAGACCATACATAAAAAAGCCGGGGATAATTCCCATAAGCGCTAACTTAAGGGTCGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATCCCTGGTCAGCAGTTCTg                                                  >  1:298717/1‑143 (MQ=255)                                                                                      tCGCAGCACTGGTGCTGTAAGACCATACATAAAAAAGCCGGGGATAATTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTgg                                                 >  2:583675/1‑143 (MQ=255)                                                                                      ccGCAGCACTGGTGATGTAAGCCCAGACATAAAAAAGCCGGGGATAATTCCCATAAGCGCTAACTTAAGGGTTGTGTTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTgg                                                 <  1:132672/142‑1 (MQ=255)                                                                                       cGCAGCACTGGTGCTGTAAGACCATACATAAAAAAGCCGGGGATAATTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTGGc                                                <  1:87952/143‑1 (MQ=255)                                                                                          aggattGGTGTTGTAAGACCATACATAAAAAAGCCGGGGATAATTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTGGCCCa                                             <  2:32753/138‑1 (MQ=255)                                                                                               gggTGCTGCAAGCCCATACCTAAAAAAGCCGGGGATAATTCCCCTAAGCGCGACCGTAAGGGATGTGTTAGTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTGGCCCATATTg                                        <  1:566450/142‑1 (MQ=17)                                                                                                 gtggcgTAAGACCATACATAAAAAAGCCGCGGATAATTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTTATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTGGCCCATATTGGt                                      <  2:928638/138‑1 (MQ=255)                                                                                                 gTGCTGTAAGACCATACATAAAAAAGCCGGGGATAATTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTGGCCCATATTGGt                                      >  1:455131/1‑143 (MQ=255)                                                                                                   gCTGTAAGACCATACATAAAAAAGCCGGGGATAATTCCCATAAGCGCTAACTTAAGGGTCGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTGGCCCCTATCGGtcc                                    >  2:989472/1‑141 (MQ=255)                                                                                                    cTGTAAGACCATACGTAGAAGAGCCGGGGATAATTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTGGCCCATATTGGTAAg                                   <  2:361944/143‑1 (MQ=255)                                                                                                       tAAGACCATACATAAAAAAGCCGGGGATAATTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTGGCCCATATTGGTAAGccc                                >  1:790023/1‑143 (MQ=255)                                                                                                          gACCATACATAAAAAAGCCGGGGATAATTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGAAATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATCCTGGCCCATCTTGGCAAGccctga                             >  2:1988/1‑140 (MQ=17)                                                                                                          gACCATAAATAAACAAGCCGGGTATGCTTCCCATAAGCGCTAACTTAGCTGTGGTGGTATTACGCCTGATATGATTTAACGTTCCCATGAATTACTCTCACGATACCTTGTCAGCAATTCTTGCCCATATTGGTAAGCCCtaa                             <  2:912517/143‑1 (MQ=14)                                                                                                                 cataAAAAAGCCGGGGATAATTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCAAGATAACTCGACAGCAACTCTGGCCCACATTGGAAAGCCCGAAGAACTgg                      >  2:583532/1‑143 (MQ=14)                                                                                                                  ataAAAAAGCCGGGGATAATTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTGGCCCATATTGGTAAGCCCGAAGCACTGGa                     >  1:742484/1‑143 (MQ=255)                                                                                                                    aaaaaaGCCGGGGATAATTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCAATATAACTGGTACGCAATTCTGGCCCATCTTTGTAAGCCCGCAGAACGGGATa                   >  2:602292/1‑143 (MQ=14)                                                                                                                              gggATAATTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTGGCCCATATTGGTAAGCCCGAAGAACTGGATACTTCGGCACg         >  1:475708/1‑143 (MQ=35)                                                                                                                               ggATAATTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTGGCCCATATTGGTAAGCCCGAAGAACTGGATACTTCGGCCCGt        >  2:934976/1‑143 (MQ=25)                                                                                                                                gATAATTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTGGCCCATATTGGTAAGCCCCAAGAACTGGATACTTCGGACCGTa       >  2:202193/1‑143 (MQ=255)                                                                                                                                 aTAATTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTGGCCCATATTGGTAAGCCCGAAGAACTGGATACTTCGGCACGTaa      >  1:788067/1‑143 (MQ=33)                                                                                                                                     ttCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTGGCGTGTATTGGTAAACCCGAAGAACTGGATACTTCGGCACGTAATGcc  >  2:486462/1‑143 (MQ=2)                                                                                                                                       |                                                                                                                                             AATGAAGAGCAAGGTAACGTGGTTTCTCGCTTCGGTCTGAAGCTGGTTTACGGCTCTACCCTGGTGAGTGTGCTGTCTGCGTCAATCGCAGCACTGGTGCTGTAAGACCATACATAAAAAAGCCGGGGATAATTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTGGCCCATATTGGTAAGCCCGAAGAACTGGATACTTCGGCACGTAATGCC  >  NC_000913/2514140‑2514414

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.