Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC JC	NC_000913	2,536,312	Δ1 bp :: IS186 (+) +6 bp :: Δ1 bp	coding (479‑484/510 nt)	crr →	glucose‑specific enzyme IIA component of PTS

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	2514274 =	NA (NA)	47 (0.790)	41/278	0.4	100%	noncoding (2/1345 nt)	IS186	repeat region
	?	NC_000913	= 2536317	0 (0.000)					coding (484/510 nt)	crr	glucose‑specific enzyme IIA component of PTS
*	?	NC_000913	= 2515616	NA (NA)	64 (1.060)	47/280	0.2	100%	noncoding (1344/1345 nt)	IS186	repeat region
	?	NC_000913	2536312 =	0 (0.000)					coding (479/510 nt)	crr	glucose‑specific enzyme IIA component of PTS

GAGCTTACAGGTGGCCATTCGTGGGACAGTATCCCTGACAGCCTACAAAACGCAATTGAAGAACGCGAGGCATCGTCTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGATAATTCCCCGGTTTTTTTACGCCTGTTAATCAGCTAATGGCTGCGGGCGACCAATCAAATACCCTTGCAGATATTGCACCCCGAGCTTATGCAATAGCGCCTGCTGCTGTTGCGTCTCGACAAACTCCGCGACCACACTCA  >  NC_000913/2515477‑2515758                                                                                                                                            |                                                                                                                                               gAGCTTACAGGTGGCCATTCGTGGGACAGTATCCCTGGCAGCGTACAAAACGCACTTGAAGAACGCGAGGCATCGTCTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGATa                                                                                                                                             <  2:488907/143‑1 (MQ=2)      tACAGGTGGCCATTCGTGGGACAGTATCCCTGACAGCCTACAAAACGCAATTGAAGAACGCGAGGCATCGTCTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGCTAATTcc                                                                                                                                        >  2:458365/1‑143 (MQ=18)         aGGTGGCCATTCGTGGGACAGTATCCCTGACAGCCTACAAAACGCAATTGAAGAACGCGAGGCCTCGTCTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGATTAAAAAATTAAATAAGCGCTTATGAGATAATACCCCg                                                                                                                                     >  1:867734/1‑143 (MQ=11)         aGGTGGCCATTCGTGGGACAGTATCCCTGACAGCCTACAAAACGCAATTGAAGAACGCGAGGCATCGTCTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGATAATTCCCCg                                                                                                                                     <  2:194292/143‑1 (MQ=35)           gTGGCCATTCGTGGGACAGTATCCCTGACAGCCTACAAAACGCAATTGAAGAACGAGAGGCATCGTCTTAACGAGGCACCGAGGCATCGCATTGTTCAGATGGTTCCACCCTTCAGTTAGCGCTTATGAGATAATTCCCCGGt                                                                                                                                   >  2:562019/1‑143 (MQ=11)               ccATTCGTGGGACAGTATCCCTGACAGCCTACAAAACGCAATTGTAGTTCGCGTGGCATCGTCTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGATAATTCCCCGGttttt                                                                                                                               <  1:367159/143‑1 (MQ=255)                 aTTCGTGGGACAGTATCCCTGACAGCCTACAAAACGCAATTGAAGAACGCGAGGCATCGTCTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGATCATTCCCCGGtgttttt                                                                                                                             >  2:800741/1‑143 (MQ=18)                       tggATAGTATCTTTGACAGTCTACAAAACGCAATTGAAGAACGCGAGGCATCGTCTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTTCAACCTTTAAGTTAGCGCTTATGGGATAATTCCCCGGTTTTTTTACGCCt                                                                                                                       <  2:568604/142‑1 (MQ=14)                       gggCCAGTATCCCTGACAGCCTACAAAACGCAATTGAAGAACGCGAGGCATCGTCTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGATAATTCCCCGGTTTTTTTACGCCt                                                                                                                       <  2:588387/143‑1 (MQ=255)                         gACAGTATCCCTGACAGTCTACAAAACGCAATTGAAGAACGCGAGGCATCGTCTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGATAATTCCCCGGTTTTTTTACGCCTGt                                                                                                                     <  2:898152/143‑1 (MQ=255)                         gACAGTATCCCTGACAGCCTACAAAACGCAATTGAAGAACGCGAGGCCTCGTCTTCACGAGGCCCCGACGCGTCGCATTCTTCAGATGGTTCAACCCTTACGTTCGCGCTTATGGGATAATTCCCCGGGTCTTTTCCGccact                                                                                                                     >  2:118733/1‑140 (MQ=11)                              tATCCCTGACAGCCTACAAAACGCAATTGAAGAACGCGAGGCATCGTCTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCCTATGGGATAATTGCCCGGATTTTTTACGCCAGTTCAta                                                                                                                >  2:165299/1‑142 (MQ=14)                                tCCCTGACAGCCTACAAAACGCAATTGAAGAACGCGAGGCATCGTCTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGATAATTCCCCGGTTTTTTTACGCCTGTTAATCAg                                                                                                              <  1:187328/143‑1 (MQ=255)                                   cTGACAGCCTACAAAACGCAATTGAAGAACGCGAGGCATCGCCTAAACAACGCACCGAGGCGTCGCATCCTGCATATCGTTCAACCCTGAAGTTAGCGCTTATGCGATAATCCCCCGGTCTTCCTACGCCTGTTCACCCGCTa                                                                                                           >  2:92661/1‑143 (MQ=14)                                        aGCCTACAAAACGCAATTGAAGAACGCGAGGCATCGTCTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTTCAACGCTTAAGGTAGCGCTTGTGGGATAATTCCCCGGTTTTTTTCCGGCGGTAAATGACCTAATGGc                                                                                                      >  2:779708/1‑143 (MQ=14)                                        aGCCTACAAAACGCAATTGAAGAACGCGAGGCATCGTCTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTTCAACCGTTAAGTTAGCGCTTATGGGATAATTCCCCGGTTTTTTTACGCCTGTTGATCAGCTAAtagg                                                                                                      >  2:651476/1‑140 (MQ=255)                                           cTACAAAACGCGATTGAAGATCGCGATGCATCGTCTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGATAATTCCCCGGTTTTTTTACGCCTGTTAATCAGCTAATGGCTGc                                                                                                   <  1:882693/143‑1 (MQ=255)                                            tACAAAACGCAATTGAAGAACGCGAGGCATCGTCTTAACGAGGCACCGAGGCGTCGCATTCTTCAGACGGTTCAACCCTTAAGTTAGCGCTTATGGGATAATTCCCCGGTTTTTTTACGCCAGTCAATCAGCTAATGGCTGCg                                                                                                  >  1:838596/1‑143 (MQ=255)                                             aCAAACCGCACTTGCAGAACGCGAGGCATCGTCTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGATAATTCCCCGGTTTTTTTACGCCTGTTAATCAGCTAATGGCTGCgg                                                                                                 <  1:71395/143‑1 (MQ=255)                                               aaaaCGCAATTGAAGAACGCGAGGCATCGTCTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGATGATTCCCCGGTTTTTTTACGCCTGTTAATCAGCTAATGGCTGCGGGc                                                                                               >  1:1075663/1‑143 (MQ=255)                                               aaaaCGCAATTGAAGAACGCGAGGCATCGTCTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGATAATTCCCCGGTTTTTTTACGCCTGTTAATCAGCTAATGGCTGCGGGc                                                                                               >  1:1095137/1‑143 (MQ=255)                                                     cAATTGAAGAACTCGTGGGATCGTCTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGATAATTCCCCGGTTTTTTTACGCCTGTTAATCAGCTAATGGCTGCGGGCGACCaa                                                                                         <  1:871198/143‑1 (MQ=255)                                                     cAATTGAAGAACGCGAGGCATCGTCTTAACGAGGCACCGAGGCGTCGCATTATTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGATAATTCCCCGGTTTTTTTACGCCTGTTAATCAGCTAATGGCTGCGGGCGACCaa                                                                                         >  1:11355/1‑143 (MQ=255)                                                       aTTGAAGAACGCGAGGCAACGTCTGAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGATAATTCCCCGGTTTTTTTACGCCTGTTAATCAGCTAATGGCTGCGGGCGACCAATc                                                                                       <  1:4821/143‑1 (MQ=255)                                                            agacCGCGAGGCATCGTCTTAACGACGCACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGATAATTCCCCGGTTTTTTTACGCCTGTTAATCAGCTAATGGCTGCGGGCGACCAATCAAATa                                                                                  <  1:365094/143‑1 (MQ=255)                                                            agaaCGGGACGCACCGTCTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGATAATTCCCCGGTTTTTTTACGCCTGTTAATCAGCTAATGGCTGCGGGCGACCAATCAAATa                                                                                  <  1:935869/143‑1 (MQ=255)                                                             gaaCGCGAGGCATCGTCTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGATAATTCCCCGGTTTTTTTACGCCTGTTAATCAGCTAATGGCTGCGGGCGACCAATCAAATAc                                                                                 >  1:483564/1‑143 (MQ=255)                                                               aCGCGAGGCATCGTCTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGATAATTCCCCGGTTTTTTTACGCCTGTTAATCAGCTAATGGCTGCGGGCGACCAATCAAATAccc                                                                               <  2:333250/143‑1 (MQ=255)                                                                 gcgAGGCATCGTCTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGATAATTCCCCGGTTTTTTTACGCCTGTTAATCAGCTAATGGCTGCGGGCGACCACTCATATCCCCtt                                                                             >  1:135633/1‑143 (MQ=255)                                                                  cggggCATCGTCTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAGGTTAGCGCTTATGGGATAATTCCCCGGTTTTTTTACGCCTGTTCATCAGCTAATGGCTGCGGGCGACCAATCAAATACCCTTg                                                                            <  2:544434/140‑1 (MQ=255)                                                                   gAGGCATCGTCTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGATAATTCCCCGGTTTTTTTACGCCTGTTCATCCGCTAATGGCTTCTGGCGACCAATCAAATACACttac                                                                           >  1:380618/1‑141 (MQ=21)                                                                           gTCTTAACGAGACACCGAGGCGTCGCATTCTTCAGATGGATCAACCCTTAAGTTAGCGCTTATGGGATAATTCACCGGTTTTTTTACGCCTGTTAATCAGCTAATGGCTGCGGGCGACCACTCAAATACCCTTGCAGatatct                                                                   >  2:191032/1‑141 (MQ=255)                                                                             cTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGATAATTCCCCGGTTTTTTTACTCCTGTTAATCAGCTAATGGCTGCGGGTGACCAATCACATTCTCTTGCAGATATTGCa                                                                 >  2:1022852/1‑143 (MQ=25)                                                                                 aCGAGGCACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGATAATTCCCCGGTTTTTTTACGCCTGTTAATCAGCTAATGGCCGCGGGCGACCAACCTAATACCCTCGCAGCTATTGCGccac                                                             >  2:213714/1‑141 (MQ=25)                                                                                    aGGCACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGATAATTCCCCGGTTTTTTTACGCCTGTTAATCAGCTAATGGCTGCGGGCGACCAATCAAATACCCTTGCAGATATTGCACCCCGAg                                                          <  2:444322/143‑1 (MQ=255)                                                                                     ggCACCGAGGCGTCGCATTCGTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGATAATTCCCCGGTTTTTTTACGCCTGTTAATCAGCTAATGGCTGCGGGCGACCAATCAAATACCCTTGCAGATATTGCACCCCGAGc                                                         <  2:567245/143‑1 (MQ=255)                                                                                         ccGAGGCGTCGCATTCTTCAGATGGTGCAACCCTTAAGTTAGCGATTATGAGATAATTCCCCGGTTTTTTTACGCCTGTTAATCAGCTAATGGCTGCGGGCGACCAATCAAATACCCTTGCAGATATTGCACCCCGAGCTTAt                                                     <  1:1055618/143‑1 (MQ=255)                                                                                          cGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGATAATTCCCCGGTTTTTTTACGCCTGTTAATCAGCTAATGGCTGCGGGCGACCAATCAAATACCCTTGCAGATATTGCACCCGGGGCTTATg                                                    >  2:920244/1‑143 (MQ=255)                                                                                          cGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGATAATTCCCCGGTTTTTTTACGCCTGTTAATCAGCTAATGGCTGCGGGCGACCAATCAAATACCCTTGCAGATATTGCACCCCGAGCTTATg                                                    <  2:534707/143‑1 (MQ=255)                                                                                               cGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGATAATTCCCCGGTTTTTTTACGCCTGTTAATCAGCTAATGGCTGCGGGCGACCAATCAAATACCCTTGCAGATATTGCACCCCGAGCTTCTGCAATa                                               >  1:886036/1‑143 (MQ=255)                                                                                                gtctgaTTCTTCAGATGGTTCATCCCTTAACTTAGCGCTTATGGGATAATTCCCCGGTTTTTTTACGCCTGTTAATCAGCTAATGGCTGCGGGCGACCAATCAAATACCCTTGCAGATATTGCACCCCGAGCTTATGCAATAg                                              <  2:869611/138‑1 (MQ=255)                                                                                                 tCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGATAATTCCCCGGTTTTTTTACGCCTGTTAATCAGCTAATGGCTGCGGGCGACCAATCAAATATCCTTCCAGATATTGCACCAAGAGCTTACGCAATAgc                                             >  2:285214/1‑143 (MQ=38)                                                                                                  cGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATCGGATAATTCCCCGGTTTTTTTACTCCTGTTTATCAGCTAATGCCTGCGGGCGTCCAATCAAATACCCCTCCACCCATTGCCCCCCGCGCTTATGCaacaccc                                            >  2:314715/1‑138 (MQ=25)                                                                                                     aTTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGATAATTCCCCGGTTTTTTTACGCCTGTTAATCAGCTAATGGCTGCGGGCGACCAATCAAGTACCGTTGCAGATATTGCACCCGGAGCTTATGCAATGGCGCct                                         >  1:881029/1‑143 (MQ=255)                                                                                                         ttcAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGATAATTCCCCGGTTTTTTTACGCCTGTTAATCAGCTAATGGCTGCGGGCGACCAATCAAATACCCTTGCAGATATTGCACCCCGAGCTTATGCAATAGCGCctgctg                                     >  1:921186/1‑143 (MQ=255)                                                                                                             ggtGGTTCAAGCCTTAAGTTAGCGCTTATGGGATAATTCCGCGGTTTTTGTACGCCTGTTAATCAGCTAATGGCTGCGGGCGACCAATCAAATACCCTTGCAGATATTGCACCCCGAGCTTATGCAATAGCGCCTGCTGCTGt                                 <  2:1082586/141‑1 (MQ=255)                                                                                                              aTGGTTCAACCCTTAAGTTAGCGCTTATGGGATAATTCCCCGGTTTTTTTACGCCTGTTAATCAGCTAATGGCTGCGGGCGACCAATCAAATACCCTTGCAGATATTGCACCCCGAGCTTCTGCCATAGCGCCCGCTGCTGtt                                >  1:564786/1‑143 (MQ=255)                                                                                                                ggTTCAACCGTTAAGGTAGCGCTTATGGGATAATTCCCCGGTTTTCTTACGCCTGTTAATCAGCTAATGGCTGCGGGCGACCAATCAAATACCCTTGCAGATATTGCACCCCGAGCTTATGCAATAGCGCCTGCTGCTGTTGc                              <  1:69795/143‑1 (MQ=255)                                                                                                                ggTTCAACCCTTAAGTTAGCGCTTATGGGATAATTCCCCGGTTTTTTTACGCCTGTTAATCAGCTAATGGCTGCGGGCGACCAATCAAATACCCTTGCAGATATTGCACCCCGAGCTTATGCAATAGCGCCTGCTGCTGTTGc                              <  2:521767/143‑1 (MQ=255)                                                                                                                 gTTCAACCCTTAAGTTAGCGCTTATGGGATAATTCCCCGGTTTTTTTACGCCTGTTAATCAGCTAATGGCTGCGGGCGACCAATCAAATACCCTTGCAGATATTGCACCCCGAGCTTATGCAATAGCGCCTGCTGCTGTTGCg                             >  1:997139/1‑143 (MQ=255)                                                                                                                    cAACCCTTAAGTTAGCGCTTATGGGATAATTCCCCGGTTTTTTTACGCCTGTTAATCAGCTAATGGCTGCGGGCGACCAATCAAATACCCTTGCAGATATTGCGCCCTGCTCTTATGCAATAGCGCCTGCTGCTGTTGCGtct                          <  2:796392/143‑1 (MQ=255)                                                                                                                     ctcccTTCAATGAGCACTTCTGGCATAATTCCCCCGTTTTTTTACGCATGTTAATCAGCTAATGGCTGCGGCCGACCAATCAAATTCCCTTGCAGATATTGCACCCCGATCTTATTCAATAGCCCCTGCTGCTGTTGCGtctc                         <  2:827062/141‑1 (MQ=255)                                                                                                                      aCCCTTAAGTTAGCGCTTATGGGATAATTCCCCGGTTTTTTTACGCCTGTTAATCAGCTAATGGCTGCGGGCGACCAATCAAATACCCTTGCAGATATTGCACCCCGAGCTTATGCAATAGCGCCTGCTGCTGTTGCGTCTCg                        >  1:81107/1‑143 (MQ=255)                                                                                                                      aCCCTGAAGTCAGCGCTTATGCGCTAATTCCCCGGTTTTTTTACGCCTGTTAATCAGCTAATGGCTGCGGGCGACCAATCACATACCCTTGCAGTTATTGCACCCCGAGCTTATGCAATAGCGCCTGCTGCTGTTGCGTCTCg                        <  1:125044/143‑1 (MQ=255)                                                                                                                      aCCCGTAAGTTAGCGCTGATGGGATAATTCCCCGGTTGTCTTACGCCTGTTACTCAGCTAATGGCTGCGGGCGACCAATCAAATACCCTTGCAGATATTGCACCCCGAGCTTATGCAATAGCGCCTGCTGCTGTTGCGTCTCg                        <  1:125528/143‑1 (MQ=255)                                                                                                                         cTTAAGTTAGCGCTTATGGGATAATTCCCCGGTTTTTTTACGCCTGTTAATCAGCTAATGGCTGCGGGCGACCAATCAAATACCCTTGCAGATATTGCACCCCGAGCGTATGCAATAGCGCCTGCTGCTGTTGCGTATCGACa                     >  1:1001/1‑143 (MQ=255)                                                                                                                                      ttATGGGATAATTCCCCGGTTTTTTTACGCCTGTTAATCAGCTAATGGCTGCGGGCGACCAATCAAATACCCTTGCAGATATTGCACCCCGAGCTTATGCAATAGCGCCTGCTGCTGTTGCGTCTCGACAAACTCCGCGACca        <  2:483564/143‑1 (MQ=255)                                                                                                                                            gATAATTCCCCGGTTTTTTTACGCCTGTTAATAAGCTAATGGCTGCGGGCGACCAATCAAATACCCTTGCAGATATTGCACCCCGAGCTTATGCAATAGCGCCTGCTGCTGTTGCGTCTCGACAAACTCCGCGACCACACTCa  >  1:889706/1‑143 (MQ=255)                                                                                                                                            |                                                                                                                                               GAGCTTACAGGTGGCCATTCGTGGGACAGTATCCCTGACAGCCTACAAAACGCAATTGAAGAACGCGAGGCATCGTCTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGATAATTCCCCGGTTTTTTTACGCCTGTTAATCAGCTAATGGCTGCGGGCGACCAATCAAATACCCTTGCAGATATTGCACCCCGAGCTTATGCAATAGCGCCTGCTGCTGTTGCGTCTCGACAAACTCCGCGACCACACTCA  >  NC_000913/2515477‑2515758

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.