Predicted mutation
evidence seq id position mutation annotation gene description
JC JC NC_000913 2,536,312 Δ1 bp :: IS186 (+) +6 bp :: Δ1 bp coding (479‑484/510 nt) crr → glucose‑specific enzyme IIA component of PTS

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 2514274 =NA (NA)47 (0.790) 41/278 0.4 100% noncoding (2/1345 nt) IS186 repeat region
?NC_000913 = 2536317 0 (0.000)coding (484/510 nt) crr glucose‑specific enzyme IIA component of PTS
* ? NC_000913 = 2515616NA (NA)64 (1.060) 47/280 0.2 100% noncoding (1344/1345 nt) IS186 repeat region
?NC_000913 2536312 = 0 (0.000)coding (479/510 nt) crr glucose‑specific enzyme IIA component of PTS

Only 100 of 114 total aligned reads displayed.

CGCGTGAAAGTTGGCGATACTGTCATTGAATTTGATCTGCCGCTGCTGGAAGAGAAAGCCAAGTCTACCCTGACTCCGGTTGTTATCTCCAACATGGACGAAATCAAAGAACTGATCAAACTGTCCGGTAGCGTAACCGTGGGTGAAACCCCGGTTATCCGCATCAAGAAGTAATTCTTGCCGCAGTGAAAAATGGCGCCCATCGGCGCCATTTTTTTATGCTTCCGCCAGCGGCGGCAAAATCAATTCATCGCTCTCATGCTGCTGGGTGTAGCGCATCACTTC  >  NC_000913/2536170‑2536454
                                                                                                                                              |                                                                                                                                              
tggcgcttacaggtggccaggcgggggacagtatccctgacagcctacaaaacgcaattgaagaacgcgaggcatcgtcttaacgaggcaccgaggcgtcgcattcttcagatggttcaacccttaagttagcgcttatgggg                                                                                                                                                <  2:942702‑M2/1‑1 (MQ=255)
ctgagcttacaggtggccactcgtgggacagtatcgctgacagcctacaaaacgcaattgaagaacgcgaggcatcgtcttaactaggcaccgaggcgtcgtattcttcagatggttcaacccttaagttagcgcttatgggg                                                                                                                                                <  2:248241‑M2/1‑1 (MQ=255)
 ggagcttacaggtggccattcgtgggacagtatccctgacagcctacaaaacgcaattgaagaacgcgaggcatcgtcttaacgaggcaccgaggcgtcgcattcttcagatggttcaacccttaagttagcgcttatgggGt                                                                                                                                               >  1:3635‑M2/142‑143 (MQ=255)
 ggagcttacaggtggccattcgtgggacagtatccctgacagcctacaaaacgcaattgaagaacgcgaggcatcgtcttaacgaggcaccgaggagccgcattcttcatgttgttcaacccttaagttagcgcttatgggGt                                                                                                                                               <  1:211928‑M2/2‑1 (MQ=255)
 ggagcttacaggtggccattcgtgggacagcacccctgacagcctacaaaacgcaattgaagaacgcgaggcatcgtcttaacgaggcaccgaggcgtcgcattcttcagatggttcaacccttaagttagcgcttatgggGt                                                                                                                                               <  1:781088‑M2/2‑1 (MQ=255)
 gcttcaaaGTTGGCGTTACTGTCATTGATTTTGATCTGTTGCTGCTGGAAGAGAAAGCCAAGTCTACCGTGGCACCGGTTGTTATCTCCAACATGGACGAAATCAAAGAACTGATCAAACTGTCCGGTAGCGTAACCGTGGGt                                                                                                                                               <  1:647324/138‑1 (MQ=255)
          aggtggcaatgcgtgggacagtatccctgacagccgacaaaacggaattgaggcacgcgaggcatcgtcttaacgtggcaccgaggcgtcgcattcttcagatggttcaacccttaagttagtgcttataggGTGAAACCCCg                                                                                                                                      <  2:198291‑M2/11‑1 (MQ=255)
           ggtggccattcgtgggacagtatccctgacggcctacaaaacgcaattgaagaacgcgaggcatcgtcttaacgaggcaccgaggcgtcgcattcttcagatggttcaacccttaagttagcgcttatgggGTGAAACCCCgg                                                                                                                                     <  1:459139‑M2/12‑1 (MQ=255)
           ggtggccattcgtgggacagtatccctgacagcctacaaaacgcaattgaagaacgcgaggcatcgtcttaacgaggcaccgaggcgtcgcattcttcagatggttcaacccttaagttagcgcttatgggGTGAAACCCCgg                                                                                                                                     <  1:541536‑M2/12‑1 (MQ=255)
            gtggccattcgtgggacagtatccctgacagcctacaaaacgcaattgaagaacgcgaggcatcgtcttaacgaggcaccgaggcctcgcattcttcagatggttcaacccttaagttagcacttatggaGTGAAACACCGGt                                                                                                                                    >  1:349993‑M2/131‑143 (MQ=255)
            ggCGATACTGTCATTGAATTTGATCTGCCGCTGCCGGAAGAGAAAGCCAAGTCTACCCTGACTCCGGTTGTTATCTCCAACATGGACGAAATCAAAGAACTGATCAAACTGTCCGGTAGCGTAACCGTGGGTGAAAcccataa                                                                                                                                    >  1:366559‑M2/1‑136 (MQ=255)
                ctaCTGTCATTGAATTTGATCTGCCGCTGCTGGAAGAGAAAGCCAAGTCTACCCTGACTCCGGTTGTTATCTCCAACATGGACGAAATCAAAGAACTGATCAAACTGTCCGGTAGCGTAACCGTGGGTGAAAcccataagcgc                                                                                                                                >  2:298838‑M2/2‑132 (MQ=255)
                 cattcgtgggacagtatccctgacagcctacaaaaagcaattgaagaacgcgaggcatcgtcttaacgaggcaccgaggcgtcgcattcttcagatggttcaacccttaggttagcgcttatgggGTGAAACCCCGGTTATcc                                                                                                                               >  1:177347‑M2/126‑143 (MQ=255)
                    tGTCATTGAATTTGATCTGCCGCTGCTGGAAGAGAAAGCCAAGTCTACCCTGACTCCGGTTGTTATCTCCAACATGGACGAAATCAAAGAACTGATCAAACTGTCCGGTAGCGTAACCGTGGGTGAAAcgcataagcgctcac                                                                                                                            <  1:224912‑M2/143‑16 (MQ=255)
                     cgggggacggtgtccctgacggcctacaatacgcaatggaagaacgcgaggcatcgtcttaacgaggcaccgaggcgtcgcattcttcagatggttcaacccttaagttagcgcttatgggGTGAAACCCCGGTTATCCGCAt                                                                                                                           <  2:1099667‑M2/22‑1 (MQ=255)
                      tCATTGAATTTGATCTGCCGCTGCTGGAAGAGAAAGCCAAGTCTACCCTGACTCCGGTTGTTATCGCCAACATGGAGGAAATCAAAGAACTGATCAAACTGTCCGGTAGCGTAACCGTGGGTGCAGcccatgagcgctaactt                                                                                                                          <  1:303201‑M2/143‑18 (MQ=255)
                      gtgggacagtatccctgacagcctacaaaacgcaattgaagaacgcgaggcatcgtcttaacgaggcaccgaggcgtcgcattcttcagatggttcaacccttacgttcgcgcttatgggGTGAAAACCCGGATATCAGCata                                                                                                                          >  1:435224‑M2/121‑142 (MQ=255)
                        gggacagtatccctgccagcctacaaaacgcaattgaagaacgcgaggcatcgtcttaacgaggcaccgaggcgtcgcattcttcagatgtttcaacccttaagttagcgcttatgggGTGAAACCCCGGTTATCCGCATCaa                                                                                                                        <  1:315420‑M2/25‑1 (MQ=255)
                          gacagtatccctgacagcctacaaaacgcaattgaagaacgcgaggcatcgtcttaacgaggcaccgaggcgtcgcattcttcagatggttcaacccttaagttagcgcttatgggGTGAAACCCCGGTTATCCGCATCaaga                                                                                                                      >  1:303832‑M2/117‑143 (MQ=255)
                          gacagtatccccgacagcctacaaaacgcaattgaagaacgcgaggcatcgtcttaacgaggcaccgaggcgtcgcattcttcagatggttcaacccttaagttagcgcttatgggGTGAAACCCCGGTTATCCGCATCaaga                                                                                                                      <  1:272709‑M2/27‑1 (MQ=255)
                           gAATTTGATCTGCCGCTGCTGGAAGAGAAAGCCAAGTCTACCCTGACTCCGGTTGTTATCTCCAACATGGACGAAATCAAAGAACTGATCAAACTGTCCGGTAGCGTAACCGTGGGTGAAAcccataagcgctcacttaaggg                                                                                                                     <  2:765761‑M2/143‑23 (MQ=255)
                           acggtatcccttacagcctacacaacgcaatggaagaacgcgaggcatcgtcttaacgaggcaccgaggcgtcgcattcttcagatggttcaacccttaagttagcgcttatgggGTGAAACCCCGGTTATCCGCATCaagaa                                                                                                                     <  2:112456‑M2/28‑1 (MQ=255)
                           acagtatccctgacagcctgcaaagcgcaattgaagaacgcgaggtagcgtcttaacgaggcaccgaggcggcgcattcttcagatggttcaacccttaagttagcgcttatgggGTGAAACCCCGGTTATCCGCATCaagaa                                                                                                                     <  2:74453‑M2/28‑1 (MQ=255)
                           acagtatccctcacagcccacaaaacgcaattgaagaacgcgaggcatcgtcttaacgaggcaccgaggcgtcgcattcttcagatggttcaacccttaagttagcgcttatgggGTGAAACCCCGGTTATCCGCATCaagaa                                                                                                                     <  1:413802‑M2/28‑1 (MQ=255)
                               ttGATCTGCCGCTGCTGGAAGAGAAAGCCAAGTCTACCCTGACTCCGGTTGTTATCTCCAACATGGACGAAATCAAAGAACTGAGCAAACTGTCCGGTAGCGTAACCGTGGGTGAAAcccataagcgctaacttaagggttgt                                                                                                                 >  1:893947‑M2/1‑117 (MQ=255)
                               tggcgcggccggggcGGGAAGAGAAGGCCAGGTCTACCCTGGCGCCGGTTGTTATCTCCAACATGGACGAAATCAAAGAACTGATCAAACTGTCCGGTAGCGTAACCGTGGGTGAAAcccataagcgctaacttaagggttgt                                                                                                                 >  2:1060845‑M2/8‑117 (MQ=255)
                               tatccctgacagcctacaaaacgcaattgaagaacgcgaggcatcgtcttaacgaggcaccgaggcgtcgcattcttcagatggttcgacgcttaagttagcgcttatgggGTGAAACCCCGGTTATCCGCATCACGAAGTaa                                                                                                                 >  1:74219‑M2/112‑143 (MQ=255)
                               tagccctgacagccgacaaaacgcaattgaagaacgcgaggcatcgtcttaacgaggcaccgaggcgtcgcattcttcagatggttcaacctttaagttagcgcttatgggGTGAAACCCCGGTTATCCGCATCAAGAAGTaa                                                                                                                 <  1:45973‑M2/32‑1 (MQ=255)
                                 tccctgacagcctacaaaacgcaattgaagaacgcgaggcatcgtcttaacgaggcaccgaggcgtcgcattcttcagatggttcaacccttaagttagcgcttatgggGTGAAACCCCGGTTATCCGCATCAAGAAGTAAtt                                                                                                               >  1:394952‑M2/110‑143 (MQ=255)
                                 gATCTGCCGCTGCTGGAAGAGAAAGCCAAGTCTACCCTGACTCCGGTTGTTATCTCCAACATGGACGAACTCCAAGACCTGTTCCACCTTTCCGCTAGCGTAACCGTGAGTTAAAcccataagcactaacttaaggattgaag                                                                                                               <  1:185896‑M2/143‑29 (MQ=255)
                                 gATCTGCCGCTGCTGGAAGAGAAAGCCAAGTCTACCCTGACTCCGGTTGTTATCTCCAACATGGACGAAATCAAAGAACTGATCAAACTGTCCGGTAGCGTAACCGTGGGTGAAAcccataagcgctaccttaagggctgtgg                                                                                                               <  1:952284‑M2/143‑29 (MQ=255)
                                 accctgaccgcctacaaaacgcaattgaagaacgcgaggcatcgtcttaacgaggcaccgaggcgtcgcattcttcagatggttcaacccttaagttagcgcttatgggGTGAAACCCCGGTTATCCGCATCAAGAAGTAAtt                                                                                                               <  1:873692‑M2/34‑1 (MQ=255)
                                 accatgacagccaacaaaacgcaattgaagaacgcgaggcatcgtcttaacgaggcaccgaggcgtcgcattcttcagatggctcaacccttaagttagcgcttatgggGTGAAACCCCGGTTATCCGCATCAAGAAGTAAtt                                                                                                               <  1:1097269‑M2/34‑1 (MQ=255)
                                  aTCTGCCGCTGCTGGAAGAGAAAGCCAAGTCTACCCTGACTCCGGTTGTTATCTCCAACATGGACGAAATCAAAGAACTGATCAAACTGTCCGGTAGCGTAACCGTGGGTGAAAcccataagcgctaacttaagggttgtggt                                                                                                              >  1:951531‑M2/1‑114 (MQ=255)
                                    ctgacagcctacaaaacgcaattgaagaacgcgaggcatcgtcttaacgaggcaccgaggcgtcgcattcttcagatggttcaacccttaagttagcgcttatgggGTGACACCCCGGTTATCCGCATCCAGACGTAATTCtt                                                                                                            >  1:893820‑M2/107‑143 (MQ=255)
                                       cggctgttgaAAGAGAAAGGCAAGTCTACCCCGACTCCGGTTGTTATCTCCAACATGGACGAAATCAAAGAACTGATCAAACTGTCCGGTAGCGTAACCGTGGGTGAAAcccataagcgctaacttaagggttgtggtattac                                                                                                         >  1:927469‑M2/3‑109 (MQ=255)
                                          gcatacaaaacgaaattgaagaaaacgacgcatcgtcttaacgaggcaccgaggcgtcgcattcttcagatggttcaacccttaagttagcgcttatgggGTGAAACCCCGGTTATCCGCATCAAGAAGTAATTCTTGCCGCa                                                                                                      <  1:961762‑M2/43‑1 (MQ=255)
                                           cctacaaaacgcaattgaagaacgcgaggcatcgtcttaacgaggcaccgaggcgtcgcattcttcagatggttcagcccttaagttagcgcttatgggGTGAAACCACGGTTATCGGCAGCAAGAAGTAATTCTGGCcgccg                                                                                                     >  2:307704‑M2/100‑141 (MQ=255)
                                             ctgGAAGAGAAAGCCAAGTCTACCCTGACGCCGGTTGGTATCTCCAACATGGACGAAATCAAAGAACTGAGCAAACTGTCCGGTAGCGTAACCGTGGGTGAAAcccataagcgctaacttaagggttgtggtattacgcctga                                                                                                   >  2:529052‑M2/1‑103 (MQ=255)
                                              tgGAAGAGAAAGCCAAGTCTACCCTGACTCCGGTTGTTATCTCCAACATGGACGAAATCAAAGAACTGATCAAACTGTCCGGTAGCGTAACCGTGGGTGAAAcccctaaatccgaagtgaagggttgtggtattacgcctgat                                                                                                  <  1:1050329‑M2/143‑42 (MQ=255)
                                               caaaacgcaattgaagaacgcgaggcatcgtcttaacgaggcaccgaggcgtcgcattcttcagatggttcaacccttaagttagcgcttatgggGTGAAACCCCGGTTATCCGCATCAAGAAGTAATTCTTGCCGCAGTGaa                                                                                                 >  1:17013‑M2/96‑143 (MQ=255)
                                                  agagAAAGCCAAGTCTACCCTGACTCCGGTTGTTATCTCCAACATGGACGAAATCAAAGAACTGATCAAACTGTCCGGTAGCGTAACCGTGGGTGAAAcccataagcgctaacttaagggttgtggtattacgcctgatatga                                                                                              <  1:724592‑M2/143‑46 (MQ=255)
                                                     gaAAGCCAAGTCTACCCTGACTCCGGTTGTTATCTCCAACATGGACGAAATCAAAGAACTGATCAAACTGTCCGGTAGCGTAACCGTGGGTGAAAcccataagagctaacttaagggtggtggtattagccctgatatgattc                                                                                           <  2:465667‑M2/143‑49 (MQ=255)
                                                      caattgtataacgctatgcatcgtcttaactaggcatcgaggcgtcgcattcttcagatggttcaacccttaagttagcgcttatgggGTGAAACCCCGGTTATCCGCATCAATAAGTAATTCTTGCCGCAGTGAAAAATGgc                                                                                          <  2:557452‑M2/55‑1 (MQ=255)
                                                      caatggaagagcgcggtgcatcgtcttaacgtggcagcgaggcgtcgcattcttcagatggttcaacccttaagttagcgcttatgggGTGAAACCCCGGTTATCCGCATCAAGAAGTAATGCTTGCCGCAGTGAAAAATGgc                                                                                          <  2:949126‑M2/55‑1 (MQ=255)
                                                       aattgaagaacgcgaggcatcgtcttaacgaggcaccgaggcgtcgcattcttcagatggttcaacccttaagttagcgcttatgggGTGAAACCCCGGTTATCCGCATCAAGAAGTAATTCTTGCCGCAGTGAAAAATGgcg                                                                                         <  1:784303‑M2/56‑1 (MQ=255)
                                                       aattgaagaacgcgaggcatcgtcttaacgaggcaccgaggcgtcgcattcttcagatggttcaacccttaagttagcgcttatgggGTGAAACCCCGGTTATCCGCATCAAGAACAAATTCTTGCCGCAGTCAAAACTGgcg                                                                                         >  2:778466‑M2/88‑143 (MQ=255)
                                                           gaagaacgcgaggcatcgtcttaacgaggcaccgaggcgtcgcattcttcagatggttcaacccttaagttagcgcttatgggGTGAAACCCCGGTTATCCGCATCAAGAAGTAATTCTTGCCGCAGTGAAAAATGGCGCCCa                                                                                     >  1:1104995‑M2/84‑143 (MQ=255)
                                                              gaacgcgaggcatcgtcttaacgaggcaccgaggcgtcgcattcttcagatggttcaacccttaagttagcgcttatgggGTGAAACCCCGGTTAACCGCATCAAGAAGTAATTCTTGCCGCAGTGAAAAATGGCGCCCATCg                                                                                  >  1:797094‑M2/81‑143 (MQ=255)
                                                                    gaggcatcgtcttaacgaggcaccgaggcgtcgcattcttcagatggttcaacccttaagttagcgcttatgggGTGAAACCCCGGTTATCCGCATCAAGAAGTAATTCTTGCCGCAGTGAAAAATGGCGCCGATGGGCGCCa                                                                            <  1:338439‑M2/69‑1 (MQ=255)
                                                                    gaggcatcgtctgaacgagggaccgaggcgtcgcattcgtcagatggttcaacccttaagttagcgcttatgggGTGAAACCCCGGTTATCCGCATCCAGAAGTAATTCTTGCCGCAGTGAAAAATGGCGCCCATcggcgcta                                                                            <  1:550125‑M2/69‑3 (MQ=255)
                                                                    ccTGACTCCGGTTGTTATCTCCAACATGGACGAAATCAAAGAACTGATCAAACTGTCCGGTAGCGTAACCGTGGGTGAAAcccataagcgctaacttaacggttgtggtattactcctgatatgatttaacgtgccgatgaat                                                                            <  2:1040807‑M2/143‑64 (MQ=255)
                                                                       gcatcgtcttaacgaggcaccgaggcgtcgcattcttcagatggttcaacccttaagttagcgcttatgggGTGAAACCCCGGTTATCCGCATCAAGAAGTAATTCTTGCCGCAGTGAAAAATGGCGCCCATCGGCGCCAttt                                                                         <  1:815251‑M2/72‑1 (MQ=255)
                                                                         cTCCGGTTGTTCTCTCCAACATGGACGAAATCAAAGAACTGATCAAACTGTCCGGTAGCGTAACCGTGGGTGAAAcccatatgcgctaacttaagggttgtggtattacgcctgatatgatttaacgtgccgatgaattactc                                                                       >  1:379875‑M2/1‑75 (MQ=255)
                                                                         atcgtcttaacgaggcaccgtggcgtcgcattcttcagctggttcaacccttatgttagcgcttatggcGTGCCCCCCTGGTTATCCGCATCAAGAAGTAATTCTTGCCGCAGTGAAAAATGGTGCCTATCGGCGCCAttttt                                                                       <  2:416838‑M2/74‑1 (MQ=255)
                                                                         atcgtcttaacgaggcaccgaggcgtcgcattcttcagatggttcaacccttaagttagcgcttatgggGTGCAACCCCGGTTATCCGCATCAAGAAGTAATTCTTGCCGCAGTGAAAAATGGCGCCCATCGGCGCCAttttt                                                                       >  2:632236‑M2/70‑143 (MQ=255)
                                                                         atcgtcttaacgaggcaccgaggcgtcgcattcttcagatggttcaacccttaagttagcgcttatgggGTGAAACCCCGGTTATCCGCATCAAGAAGTAATTCTTGCCGCAGTGAAAAATGGCGCCCATCGGCGCCAttttt                                                                       <  1:839550‑M2/74‑1 (MQ=255)
                                                                          tCCGGTTGTTATCTCCAACATGGACGAAATCAAAGAACTGATCAAACTGTCCGGTAGCGTAACCGTGGGTAAAAcccataagcgctaactcaagggttgtggtattacgcctgatatgatttaacgtgccgatgaattacact                                                                      <  2:843120‑M2/143‑70 (MQ=255)
                                                                            gtcttaacgaggcaccgaggcggcgcattctccaggtggttgaacccataagttagcgcttatgggGTGAAACCCCGGTTATCCGCATCAAGAAGTAATTCTTGCCGCAGTGAAAAATGGCGCCCATCGGCGCCATTTTTTTa                                                                    <  1:93032‑M2/77‑1 (MQ=255)
                                                                              gttgttATCTCCAACATGGACGAAATCAAAGAACTGATCAAACTGTCCGGTAGCGTAACCGTGGGTGAAAcccataagcgctaacttaagggttgtggtattacgcctgatatgatttaacgtgccgatgaattactctcacg                                                                  <  2:907677‑M2/143‑74 (MQ=255)
                                                                               ttgttATCTCCAACATGGACGAAATCAAAGAACTGATCAAACTGTCCGGTAGCGTAACCGTGGGTGAAAcccataagcgctaacttaagggttgtggtattacgcctgatatgatttaacgtgccgatgaattactctcacga                                                                 <  2:758596‑M2/143‑75 (MQ=255)
                                                                               ttaacgaggcaccgaggcgtcgcattcttcagatggttcaacccttaagttagcgcttatgggGTGAAACCCCGGTTATCCGCATCAAGAAGTAATTCTTGCCGCAGTGAAACATGGCGCCCATCGGCGCCCTTTTTTTATGc                                                                 >  2:883313‑M2/64‑143 (MQ=255)
                                                                                   tATCTGCAACATGGACGAAATCGAAGAACTGATCAAACTGGCCGGTAGCGTAACCGTGGGTGAAAcccagaagcgctaacttaagggttgtggtattacggctgatatgatttaacgtgccgatgaattactctcacgataac                                                             >  1:1017060‑M2/1‑65 (MQ=255)
                                                                                    gaggcaccgaggcgtcgcattcttcagatggttcaacccttaagttagcgcttatgggGTGAAACCCCGGTTATCCGCATCAAGAAGTAATTCTTGCCGCAGTGAAAAATGGCGCCCATCGGCGCCATTTTTTTATGCTTCCg                                                            >  2:1056821‑M2/59‑143 (MQ=255)
                                                                                    gaggcaccgaggcgtcgcattcttcagatggttcaacccttaagttagcgcttatgggGTGAAACCCCGGTTATCCGCATCAAGAAGTAATTCTTGCCGCAGTGAAAAATGGCGCCCATCGGCGCCATTGTTGAATGCTTCCg                                                            >  2:61600‑M2/59‑143 (MQ=255)
                                                                                    gaggcaccgaggcgtcgcatgcttcagatggttcaccccttaagtcagcgcttatgggGTGAAACCCCGGTTATCCGCATCAAGAAGTAATTCTTGCCGCAGTGAAAAATGGCGCCCATCGGCGCCATTTTTTTATGCTTCCg                                                            <  1:352093‑M2/85‑1 (MQ=255)
                                                                                      ctcCAACATGGACGAAATCAAAGAACTGATCAAACTGTCCGGTAGCGTACCCGTGGGTGAAAcccataagcgctaacttaagggatgtggtagagcgcctgataggctttaacgtaccgatgaattaccctcacgataactgg                                                          <  2:363264‑M2/143‑82 (MQ=255)
                                                                                         cAACATGGACGACATCAAAGAACTCATCAAACTGTCCGGGAGCGTAACCGTGGGTGAAAcccataagcgctaacttaagggttgtggtattacgcctgatatgatttaacgtgccgatgaattactctcacgataactggtca                                                       >  1:67229‑M2/1‑59 (MQ=255)
                                                                                         accgaggcgtcgcattcttcagatggttcaacccttaagttagcgcttatgggGTGAAACCCCGGTTATCCGCATCAAGAAGTAATTCTTGCCGCAGTGAAAAATGGCGCCCATCGGCGCCCTTTTTTTATGCTTCCGCCAgc                                                       >  1:785654‑M2/54‑143 (MQ=255)
                                                                                         accgaggcgtcgcattcttcagatggttcaacccttaagttagcgcttatgggGTGAAACCCCGGTTATCCGCATCAAGAAGTAATTCTTGCCGCAGTGAAAAATGGCGCCCATCGGCGCCATTTTTTTATGCTTCCGCCAgc                                                       >  1:615007‑M2/54‑143 (MQ=255)
                                                                                             aggcgtcgcattcttcagatggttcaacccttaagttagcgcttatgggGTGAAACCCCGGTTATCCGCATCAAGAAGTAATTCTTGCCGCAGTGAAAAATGGCGCCCATCGGCGCCATTTTTTTATGCTTCCGCCAgcggcg                                                   <  1:941145‑M2/94‑1 (MQ=255)
                                                                                             aggcgtcgcattcttcagatggtctaacccttaagttagcgcttatgggGTGAAACCCCGGTTATCCGCATCAAGAAGTAATTCTTGCCGCAGTGAAAAATGGCGCCCATCGGCGCCATTTTTTTATGCTTCCGCCAgcggcg                                                   <  1:656022‑M2/94‑1 (MQ=255)
                                                                                                gACGAAATCAAAGAACTGATCAAACTGTCCGGTAGCGTAACCGTGGGTGAAAcccataagcgctaacttaagggttgtggtattacgcctgatatgatttaacgtgccgatgaattactctcacgataactggtcagcaattc                                                <  1:500867‑M2/143‑92 (MQ=255)
                                                                                                gACGAAATCAAAGAACTGATCAAACTGTCCGGTAGCGTAACCGTGGGTGAAAcccataagcgctaacttaagggttgtggtattacgcctgatatgatttaacgtgccgatgaattactctcacgataactggtcagcaattc                                                <  1:497958‑M2/143‑92 (MQ=255)
                                                                                                 gtcgcattcttcagatggttcaacccttaagttagcgcttatgggCTGAAACCCCGGTTATCCGCATCACGAAGTAATTCTTGCCGCAGTGAAAACTGGCGCCCATCGGCGCCATTTTTTTGTGCTTCCGCccgcggcggaca                                               >  2:779596‑M2/46‑140 (MQ=255)
                                                                                                 aCGAAATCAGAGAACTGATCAAACTGTCCGGTAGCGTAACCGTGTGTGAAAcccataagcgctaacttaagggtggtggcattacgcctgatatgatttaacgtgccgatgaattactctcacgataactggtcagcaattct                                               >  2:184397‑M2/1‑51 (MQ=255)
                                                                                                     cattcttcagatggttcaacccttaagttagcgcttatgggGTGAAACCCCGGTTATCCGCATCAAGAAGTAATTCTTGCCGCAGTGAAAAAAGGCGCACATCGGCGCCATTTTTTTATCCCTCCGCCAGCAGAGCCAAAata                                           >  1:747431‑M2/42‑142 (MQ=255)
                                                                                                      attcttcagatggttcaacccttaagttagcgcttatgggGTGAAACCCCGGTTATCCGCATCAAGAAGTAATTCTTGCCGCAGTGAAAAATGGCGCCCATCGGCGCCATTTTTTTATGCTTCCGCCAGCGGCGGCAAAACCa                                          >  2:729297‑M2/41‑143 (MQ=255)
                                                                                                      attcttcagatggttcaacccttaagttagcgcttatgggGTGAAACCCCGGTTATCCGCATCAAGAAGTAATTCTTGCCGCAGTGAAAAATGGCGCCCATCGGCGCCATTTTTTTATGCTTCCGCCAGCGGCGCCACAACCa                                          >  1:672576‑M2/41‑143 (MQ=255)
                                                                                                        cAAAGAACTGATCAAACTGTCCGGTAGCGTAACCGTGGGTGAAAcccataagcgctaacttaagggttgtggtattacgcctgatatgatttaacgtgccgatgaattactctcacgataactggtcaccaattctggcccat                                        <  2:110406‑M2/143‑100 (MQ=255)
                                                                                                         aaaGAACTGATCAAACTGTCCGGTAGCGTAACCGTGGGTGAAAcccataagcgctaacttaagggttgtggtattacgcctgatatgacttaacgtgccgatgaattactctcacgataactggtcagcaattctggcccata                                       <  2:576206‑M2/143‑101 (MQ=255)
                                                                                                              gatggtccaacccttaagttagcgcttatgggGTGAAACCCCGGGTATCCGCATCAAGAAGTAATTCTTGCCGCAGTGAAAAATGGCGCCCATCGGCGCCATTTTTTTATGCTTCCGCCAGCGGCGGCAAAATCAATTCATCg                                  <  1:621384‑M2/111‑1 (MQ=255)
                                                                                                                tGCTCCAACCGTCCGGTAGCGTAACCGTGGGTGAAAcccataagcgctaacttaagggttgtggtattacgcctgatatgatttaacgtgccgatgaattactctcacgataactggtcagcaattctggcccatattggtaa                                >  1:769186‑M2/1‑36 (MQ=255)
                                                                                                                tGATCAAACTGTCCGGTAGCGTAACCGTGGGTGAAAcccataagcgctaacttaagggttgtggtattacgcctgatatgatttaacgtgccgatgaattactctcacgataactggtcagcaattctggcccatattggtaa                                >  1:821539‑M2/1‑36 (MQ=255)
                                                                                                                  aTCAAACTGTCCGGTAGCGTAACCGTGGGTGAAAcccataagcgctaactttagggttgtggtattacgcctgatatgatttaacgtgccgatgaattactctcccgatatctggtcagcaattctggcccctattggtcagc                              <  2:403402‑M2/143‑110 (MQ=255)
                                                                                                                     aaaCTGGCCGGTAGCGTAACCGTGGGTGAAAcccataagcgctaacttaagggttgtggtattacgcctgatatgatttaacgtgccgatgaattactctcacgataactggtcagcaattctggcccatattggtaagcccg                           <  2:193797‑M2/143‑113 (MQ=255)
                                                                                                                     aaaCCGTCCGGTAGCGTAACCGTGGGTGAAAcccataagcgctaacttaagggttgtggtattacgcctgatatgatttaacgtgccgatgaattactctcacgatgactggtcagcaattctggcccatattggtaagcccg                           <  1:787476‑M2/143‑113 (MQ=255)
                                                                                                                      aaCTGTCCGGTAGCGTAACCGTGGGTGAAAcccataagcgctaacttaagggttgtggtattacgcctgatatgatttaacgtgccgatgaattactctcacgataactggtcagcaattctggcccatattggtaagcacgc                          <  2:1114342‑M2/143‑114 (MQ=255)
                                                                                                                         ccttaagttagcgcttatgggGTGAAACCCCGGTTATCCGCATCAAGAAGTAATTCTTGCCGCAGTGAAAAATGGCGCCCATCGGCGCCATTTTTTTATGCTTCCGCCAGCGGCGGCAAAATCAATTCATCGCTctcatacaa                       >  1:651778‑M2/22‑139 (MQ=255)
                                                                                                                         ccttaagttagcgcttatgggGTGAAACCCCGGTTATCCGCATCAAGAAGTAATTCTTGCCGCAGTGAAAAATGGCGCCCATCGGCGCCATTTTTTTATGCTTCCGCCAGCGGCGGCAAAATCAATTCATCGCTCTCAtgctg                       >  1:839370‑M2/22‑143 (MQ=255)
                                                                                                                              ggTAGCGTAACCGTGGGTGAAAcccataagcgctaacttaagggttgtggtattacgcctgatatgatttaacgtgccgatgaattactctcacgataactggtcagcaattctggcccatattggtaagcccgaagaactgg                  <  2:952426‑M2/143‑122 (MQ=255)
                                                                                                                               ggtagggcttaggggGTGAAACCCCCGTTATCCGCATCAAGAAGTAATTCTTGCCGCAGTGAAAAATGGCGCCCATCGGCGCCATTTTTTTATGCTTCCGCCAGCGGCGGCAAAATCAATTCATCGCTCTCATGCTGCTGGgt                 <  1:338570‑M2/128‑1 (MQ=255)
                                                                                                                                 tagcgcttatgggGTGAAACCCCGGTTATCCGCATCAAGAAGTAATTCTTGCCGCAGTGCAAAATGGCGCCCATCGGCGCCAGTTTTTTATGCTTCCGCCCGCGGCGGCAAAAGCAATTCATCCCTCCCATCCtgcggggcga               >  2:838492‑M2/14‑140 (MQ=255)
                                                                                                                                    gTAACCGTGGGTGAAAcccataagcgctaacttaagggttgttgtattacgcctgatatgatttaacttgtcgatgaattactctcacgataactggtcagcaatgctggcccatattggtaagcccgaagaactggatactt            >  1:653667‑M2/1‑16 (MQ=255)
                                                                                                                                      atccgtGTGTGAAAcccatacgcgctaccttaagggttgtggtattacgcctgatatgatttaacgtgccgatgaagtactctcacgataactggtcagcaattctggcccatattggtaagcccgaagaactggatacttcg          >  1:384989‑M2/3‑14 (MQ=255)
                                                                                                                                           gggGTGAAACCCCGGTTATCCGCATCAAGAAGTAATTCTTGCCGCAGTGAAAAATGGCGCCCATCGGCGCCATTTTTTTATGCTTCCGCCAGCGGCGGCAAAATCAATTCATCGCTCTCATGCTGCTGGGTGTAGCGCatccc     >  1:340190‑M2/4‑141 (MQ=255)
                                                                                                                                           gggGTGAAACCCCGGTTATCCGCATCAAGAAGTAATTCTTGCCGCAGTGAAAAATGGCGCCCATCGGCGCCATTTTTTTATGCTTCCGCCAGCGGCGGCAAAATCAATTCATCGCTCTCATGCTGCTGGGTGTAGCGCATCAc     >  1:485353‑M2/4‑143 (MQ=255)
                                                                                                                                             gGTGAAACCCCGGTTATCCGCATCAAGAAGTAATTCTTGCCGCAGTGAAAAATGGCGCCCATCGGCGCCATTTTTTTATGCTTCCGCCAGCGGCGGCAAAAt                                            >  2:1114629‑M2/2‑102 (MQ=255)
                                                                                                                                             gGTGAAACCCCGGTTATCCGCATCAAGAAGTAATTCTTGCCGCAGTGAAAAATGGCGCCCATCGGCGCCATTTTTTTATGCTTCCGCCAGCGGCGGCAAAAt                                            <  1:1114629‑M2/101‑1 (MQ=255)
                                                                                                                                              gTGAAACCCCGGTTATCCGCATCAAGAAGTAATTCTTGCCGCAGTGAAAAATGGCGCCCATCGGCGCCATTTTTTTATGCTTCCGCCAGCGGCGGCAAAATCAATTCATCGCTCTCATGCTGCTGGGTGTCGCGCGTCACttg  >  1:326554/1‑142 (MQ=255)
                                                                                                                                              |                                                                                                                                              
CGCGTGAAAGTTGGCGATACTGTCATTGAATTTGATCTGCCGCTGCTGGAAGAGAAAGCCAAGTCTACCCTGACTCCGGTTGTTATCTCCAACATGGACGAAATCAAAGAACTGATCAAACTGTCCGGTAGCGTAACCGTGGGTGAAACCCCGGTTATCCGCATCAAGAAGTAATTCTTGCCGCAGTGAAAAATGGCGCCCATCGGCGCCATTTTTTTATGCTTCCGCCAGCGGCGGCAAAATCAATTCATCGCTCTCATGCTGCTGGGTGTAGCGCATCACTTC  >  NC_000913/2536170‑2536454

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.