Predicted mutation
evidence seq id position mutation annotation gene description
MC JC NC_000913 759,508 (TGAACGTGCTGG)2→1 coding (803‑814/2802 nt) sucA → 2‑oxoglutarate decarboxylase, thiamine triphosphate‑binding

Missing coverage evidence...
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 759508 759519 12 24 [0] [0] 24 sucA 2‑oxoglutarate decarboxylase, thiamine triphosphate‑binding

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 7595070 (0.000)23 (0.660) 18/260 1.0 100% coding (802/2802 nt) sucA 2‑oxoglutarate decarboxylase, thiamine triphosphate‑binding
?NC_000913 759520 = 0 (0.000)coding (815/2802 nt) sucA 2‑oxoglutarate decarboxylase, thiamine triphosphate‑binding

TTCCCTGGCGCAAAACGCTTCTCGCTGGAAGGCGGTGACGCGTTAATCCCGATGCTTAAAGAGATGATCCGCCACGCTGGCAACAGCGGCACCCGCGAAGTGGTTCTCGGGATGGCGCACCGTGGTCGTCTGAACGTGCTGGTGAACGTGCTGGGTAAAAAACCGCAAGACTTGTTCGACGAGTTCGCCGGTAAACATAAAGAACACCTCGGCACGGGTGACGTGAAATACCACATGGGCTTCTCGTCTGACTTCCAGACCGATGGCGGCCTGGTGCACCTG  >  NC_000913/759366‑759647
                                                                                                                                             |                                                                                                                                            
ttCCCTGGCGCAAAACGCTTCTCGCTGGAAGGCGGTGACGCGTTAATCCCGATGCTTAAAGAGATGATCCGCCACGCTGGCAACAGCGGCACCCGCGAAGTGGTGCTCGGGATGGCGCACCGTGGTCGTCTGAACGTGCTGGg                                                                                                                                             >  1:473774‑M1/1‑142 (MQ=255)
                         tGGAAGGCGGTCACGCGTCAATCCCGATGCTTAAAGAGATGATCCGCCACGCTGGCAACAGCGGCACCCGCGAAGTGGTTCTCGGGATGGCGCACCGTGGTCGTCTGAACGTGCTGGgtaaaaaaccgcaagacttgttcgac                                                                                                                    <  1:246940‑M1/143‑27 (MQ=255)
                               gCGGTGACGCGTTAATCCCGATGCTTAAAGAGATGATCCGGCACGCTGGCAACAGCGGCACGCGCGTAGTGGTTGTGGGGATGGCGCGCCGTGGGCGTCGGAACGGGCTGGgtaaaaaaccgcaagacttgttcgacgagttc                                                                                                              >  2:528390‑M1/1‑111 (MQ=255)
                               gCGGTGACGCGTTAATCCCGATGCTTAAAGAGATGATCCGCCACGCTGGCAACAGCGGCCCCCGCGCAGTGGTTCTCGGGATGGCGCACCGTGGTCGTCTGAACGTGCTGGgtagaaaaccgcacgacgtgttcgacgagttc                                                                                                              >  2:35493‑M1/1‑111 (MQ=255)
                               gCGGTGACGCGTTAATCCCGATGCTTAAAGAGATGATCCGCCACGCTGGCAACAGCGGCACCCGCGAAGTGGTTCTCGGGATGGCGCACCGTGGTCGTCTGCACGTGCTGGgtaaaaaaccgcaagacttgttcgacgagttc                                                                                                              >  1:549145‑M1/1‑111 (MQ=255)
                                   tGACGCGTTAATCCCGATGCTTAAAGAGATGATCCGCCACGCTGGCAACAGCGGCACCCGCGAAGTGGTTCTCGGGATGGCGCACCGTGGTCGTCTGAACGTGCTGGgtaaaaaaccgcaagacttgttcgacgagttcgccg                                                                                                          <  1:27689‑M1/143‑37 (MQ=255)
                                       gcgTTAATCCCGATGCTTAAAGAGATGATCCGCCACGCTGGCAACAGCGGCACCCGCGAAGTGGTTCTCGGGATGGCGCACCGTGGTCGTCTGAACGTGCTGGgtaaaaaaccgcaagacttgttcgacgagttcgccggtaa                                                                                                      >  1:208913‑M1/1‑103 (MQ=255)
                                              gccgcatgcttAAAGAGAGGATCCGCCACTCTGGCAACAGCGGCACCCGCGAAGTGGTTCTCGGGATGGCGCACCGTGGTCGTCTGAACGTGCTGGgtaaaataccgcaagactggttcgacgagttcgccggtaaacataaa                                                                                               <  1:494144‑M1/138‑48 (MQ=255)
                                                ccGATGCTTAAAGAGATGATCCGCCACGCTGGCAACAGCGGCACCCGCGAAGTGGTTCTCGGGATGGCGCACCGTGGTCGTCTGAACGTGCTGGgtaaaaaaccgcaagacttgttcgacgagttcgccggtaaccctaaaga                                                                                             >  1:253066‑M1/1‑94 (MQ=255)
                                                 cGATGCTTAAAGAGATGATCCGCCACGCTGGCAACAGCGGCACCCGCGAAGTGGTTCTCGGGATGGCGCACCGTGGTCGTCTGAACGTGCTGGgtaaaaaaccgcaagacttgttcgacgagttcgccggtaaacataaagaa                                                                                            <  1:605379‑M1/143‑51 (MQ=255)
                                                         aaaGAGATGATCCGCCACGCTGGCAACAGCGGCACCCGCGAAGTGGTTCTCGGGATGGCGCACCGTGGTCGTCTGAACGTGCTGGgtaaaaaaccgcaaggcttgttcgacgagttcgccggtaaacataaagaacacctcgg                                                                                    >  1:318612‑M1/1‑85 (MQ=255)
                                                                 gatCCGCCACGCTGGCAACAGCGGCACCCGCGAAGTGGTTCTCGGGATGGCGCACCGTGGTCGTCTGAACGGGCTGGggaacaaaccgcaagacttgttcgacgagtgcgccgctaaacataaaagacaccccggcacgggtg                                                                            >  2:586265‑M1/1‑77 (MQ=255)
                                                                          ctctagcaacaGCCGGACCCGCGAATTGGGTCTCGGCATGGCGCACCGTGCTCGTCCGAACGTGCTGTgtaaaaaaccgcaggacttgttcgacgagttcgccggtaaacataaagaccacctcggcacgggtgacgtgaaat                                                                   <  2:622985‑M1/138‑76 (MQ=255)
                                                                                       ggCACCCGCGAAGTGGTTCTCGGGATGGCGCAACGTGGTCGCCTGAACGTGCTGGgtaaaaaaccgcaagacttgttcgacgagttcgccggtaaacataaagaacacctcggcacgggtgacgtgaaataccacatgggctt                                                      <  1:541571‑M1/143‑89 (MQ=255)
                                                                                              gggaaGTGGTGCGCGGGAGGGCGCACCGTGGGGGTCTGAAGGGGCTGGgtaaaaaaccgcaagacttgttcgacgagttcgccggtaaacataaagaacacgtcggaaggggggacgtgaaataccacatgggcttctcgtct                                               <  2:247258‑M1/141‑96 (MQ=255)
                                                                                              gcgAAGTGGTTCTCGGGATGGCGCAACGTGGTCGTCTGAACGGGCGGGgtaaaaaaccgcaagacttgttcgacgagttcgccggtaaacataaagaacacctcggcacgggtgacgtgaaataccacatgggcttctcgtct                                               <  1:85566‑M1/143‑96 (MQ=255)
                                                                                                gAAGTGGTTCTCGGGATGGCGCACCGTGGTCGTCTGAACGTGCTGGgtaaaaaaccgcaagacttgttcgacgagttcgccggtaaacataaagaacacctcggcacgggtgacgtgaaataccgcatgggcttctcgtctga                                             <  1:40917‑M1/143‑98 (MQ=255)
                                                                                                        tctcGGGATGGCGCACCGTGGTCGTCTGAACGTGCTGGgtaaaaaaccgccagacttgttcgacgagttcgccggtaaacataaagaacacctctgcacgggtgacgtgaactacgccattggcttctcgtctgacttcctct                                     >  1:421074‑M1/1‑38 (MQ=255)
                                                                                                        tctcGGGATGGCGCACCGTGGTCGTCTGAACGTGCTGGgtaaaaaaccgcaagacttgttcgacgagttcgccggtaaacataaagaacacctcggcacgggtgacgtgaaataccacatgggcttctcgtctgacttccaga                                     >  1:526804‑M1/1‑38 (MQ=255)
                                                                                                                     cACCGTGGTCGGCTGAGCGGGCTGGgtaaaaatccgcaagacgtgttcgacgaggtcgccggtaaacataaagagcacctcggcacgggtgacgtgaaataccacatgggcttctcgtctgacttccagaccgatggcggcct                        <  2:438339‑M1/143‑119 (MQ=255)
                                                                                                                     cACCGTGGGCGGCTGAGCGTGCTGGgtgaaagtccgcacgacgcgtacgacgcggtcgccggtaaacataaagagcacctcggcacgggtgacgtgaaataccacatgggcttctcgtctgacttccagaccgatggcggcct                        <  2:284681‑M1/143‑119 (MQ=255)
                                                                                                                            gtcgtcTGAACGTGCTGGgtaaaaaaccgcaagacttgttcgacgagttcgccggtaaacataaagaacacctcggcacgggtgacgtgaaataccacatgggcttctcgtctgacttccagaccgatggcggcctggtgcac                 <  1:74138‑M1/143‑126 (MQ=255)
                                                                                                                             tcgtcTGAACGTGCTGGgtaaaaaaccgcaagacttgttcgacgagttcgccggtaagcataaagaacacctcggcacgggtgacgtgaaataccacatgggcttctcgtctgacttccagaccgatggcggcctggtgcacc                >  1:413033‑M1/1‑17 (MQ=255)
                                                                                                                                           tGGgtaaaaaaccgcaagacttgttcgacgagttcgccggtaaacataaagaacacctcggcacgggtgacgtgaaataccacatgggcttctcgtctgacttccagaccgatggcggcctggtgcacgtggcgctggcgttt  >  1:630244‑M1/1‑3 (MQ=255)
                                                                                                                                             |                                                                                                                                            
TTCCCTGGCGCAAAACGCTTCTCGCTGGAAGGCGGTGACGCGTTAATCCCGATGCTTAAAGAGATGATCCGCCACGCTGGCAACAGCGGCACCCGCGAAGTGGTTCTCGGGATGGCGCACCGTGGTCGTCTGAACGTGCTGGTGAACGTGCTGGGTAAAAAACCGCAAGACTTGTTCGACGAGTTCGCCGGTAAACATAAAGAACACCTCGGCACGGGTGACGTGAAATACCACATGGGCTTCTCGTCTGACTTCCAGACCGATGGCGGCCTGGTGCACCTG  >  NC_000913/759366‑759647

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: