Predicted mutation
evidence seq id position mutation annotation gene description
MC JC NC_000913 759,508 (TGAACGTGCTGG)2→1 coding (803‑814/2802 nt) sucA → 2‑oxoglutarate decarboxylase, thiamine triphosphate‑binding

Missing coverage evidence...
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 759508 759519 12 24 [0] [0] 24 sucA 2‑oxoglutarate decarboxylase, thiamine triphosphate‑binding

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 7595070 (0.000)23 (0.660) 18/260 1.0 100% coding (802/2802 nt) sucA 2‑oxoglutarate decarboxylase, thiamine triphosphate‑binding
?NC_000913 759520 = 0 (0.000)coding (815/2802 nt) sucA 2‑oxoglutarate decarboxylase, thiamine triphosphate‑binding

AAACGCTTCTCGCTGGAAGGCGGTGACGCGTTAATCCCGATGCTTAAAGAGATGATCCGCCACGCTGGCAACAGCGGCACCCGCGAAGTGGTTCTCGGGATGGCGCACCGTGGTCGTCTGAACGTGCTGGTGAACGTGCTGGGTAAAAAACCGCAAGACTTGTTCGACGAGTTCGCCGGTAAACATAAAGAACACCTCGGCACGGGTGACGTGAAATACCACATGGGCTTCTCGTCTGACTTCCAGACCGATGGCGGCCTGGTGCACCTGGCGCTGGCGTTT  >  NC_000913/759378‑759659
                                                                                                                                              |                                                                                                                                           
ttccctggcgcaaaacgcttctcgctggaaggcggtgacgcgttaatcccgatgcttaaagagatgatccgccacgctggcaacagcggcacccgcgaagtggtgctcgggatggcgcaccgtggtcgtctgaacgtgctggg                                                                                                                                             >  1:473774‑M2/143‑143 (MQ=255)
                         tggaaggcggtcacgcgtcaatcccgatgcttaaagagatgatccgccacgctggcaacagcggcacccgcgaagtggttctcgggatggcgcaccgtggtcgtctgaacgtgctggGTAAAAAACCGCAAGACTTGTTcgac                                                                                                                    <  1:246940‑M2/26‑1 (MQ=255)
                               gcggtgacgcgttaatcccgatgcttaaagagatgatccggcacgctggcaacagcggcacgcgcgtagtggttgtggggatggcgcgccgtgggcgtcggaacgggctggGTAAAAAACCGCAAGACTTGTTCGACGAGTTc                                                                                                              >  2:528390‑M2/112‑143 (MQ=255)
                               gcggtgacgcgttaatcccgatgcttaaagagatgatccgccacgctggcaacagcggcccccgcgcagtggttctcgggatggcgcaccgtggtcgtctgaacgtgctggGTAGAAAACCGCACGACGTGTTCGACGAGTTc                                                                                                              >  2:35493‑M2/112‑143 (MQ=255)
                               gcggtgacgcgttaatcccgatgcttaaagagatgatccgccacgctggcaacagcggcacccgcgaagtggttctcgggatggcgcaccgtggtcgtctgcacgtgctggGTAAAAAACCGCAAGACTTGTTCGACGAGTTc                                                                                                              >  1:549145‑M2/112‑143 (MQ=255)
                                   tgacgcgttaatcccgatgcttaaagagatgatccgccacgctggcaacagcggcacccgcgaagtggttctcgggatggcgcaccgtggtcgtctgaacgtgctggGTAAAAAACCGCAAGACTTGTTCGACGAGTTCGCCg                                                                                                          <  1:27689‑M2/36‑1 (MQ=255)
                                       gcgttaatcccgatgcttaaagagatgatccgccacgctggcaacagcggcacccgcgaagtggttctcgggatggcgcaccgtggtcgtctgaacgtgctggGTAAAAAACCGCAAGACTTGTTCGACGAGTTCGCCGGTaa                                                                                                      >  1:208913‑M2/104‑143 (MQ=255)
                                              gccgcatgcttaaagagaggatccgccactctggcaacagcggcacccgcgaagtggttctcgggatggcgcaccgtggtcgtctgaacgtgctggGTAAAATACCGCAAGACTGGTTCGACGAGTTCGCCGGTAAACATaaa                                                                                               <  1:494144‑M2/47‑1 (MQ=255)
                                                ccgatgcttaaagagatgatccgccacgctggcaacagcggcacccgcgaagtggttctcgggatggcgcaccgtggtcgtctgaacgtgctggGTAAAAAACCGCAAGACTTGTTCGACGAGTTCGCCGGTAACCCTAAAGa                                                                                             >  1:253066‑M2/95‑143 (MQ=255)
                                                 cgatgcttaaagagatgatccgccacgctggcaacagcggcacccgcgaagtggttctcgggatggcgcaccgtggtcgtctgaacgtgctggGTAAAAAACCGCAAGACTTGTTCGACGAGTTCGCCGGTAAACATAAAGaa                                                                                            <  1:605379‑M2/50‑1 (MQ=255)
                                                         aaagagatgatccgccacgctggcaacagcggcacccgcgaagtggttctcgggatggcgcaccgtggtcgtctgaacgtgctggGTAAAAAACCGCAAGGCTTGTTCGACGAGTTCGCCGGTAAACATAAAGAACACCTCgg                                                                                    >  1:318612‑M2/86‑143 (MQ=255)
                                                                 gatccgccacgctggcaacagcggcacccgcgaagtggttctcgggatggcgcaccgtggtcgtctgaacgggctggGGAACAAACCGCAAGACTTGTTCGACGAGTGCGCCGCTAAACATAAAAGACACCCCGGCACGGGTg                                                                            >  2:586265‑M2/78‑143 (MQ=255)
                                                                          ctctagcaacagccggacccgcgaattgggtctcggcatggcgcaccgtgctcgtccgaacgtgctgtGTAAAAAACCGCAGGACTTGTTCGACGAGTTCGCCGGTAAACATAAAGACCACCTCGGCACGGGTGACGTGAAAt                                                                   <  2:622985‑M2/75‑1 (MQ=255)
                                                                                       ggcacccgcgaagtggttctcgggatggcgcaacgtggtcgcctgaacgtgctggGTAAAAAACCGCAAGACTTGTTCGACGAGTTCGCCGGTAAACATAAAGAACACCTCGGCACGGGTGACGTGAAATACCACATGGGCtt                                                      <  1:541571‑M2/88‑1 (MQ=255)
                                                                                              gggaagtggtgcgcgggagggcgcaccgtgggggtctgaaggggctggGTAAAAAACCGCAAGACTTGTTCGACGAGTTCGCCGGTAAACATAAAGAACACGTCGGAAGGGGGGACGTGAAATACCACATGGGCTTCTCGTCt                                               <  2:247258‑M2/95‑1 (MQ=255)
                                                                                              gcgaagtggttctcgggatggcgcaacgtggtcgtctgaacgggcgggGTAAAAAACCGCAAGACTTGTTCGACGAGTTCGCCGGTAAACATAAAGAACACCTCGGCACGGGTGACGTGAAATACCACATGGGCTTCTCGTCt                                               <  1:85566‑M2/95‑1 (MQ=255)
                                                                                                gaagtggttctcgggatggcgcaccgtggtcgtctgaacgtgctggGTAAAAAACCGCAAGACTTGTTCGACGAGTTCGCCGGTAAACATAAAGAACACCTCGGCACGGGTGACGTGAAATACCGCATGGGCTTCTCGTCTGa                                             <  1:40917‑M2/97‑1 (MQ=255)
                                                                                                        tctcgggatggcgcaccgtggtcgtctgaacgtgctggGTAAAAAACCGCCAGACTTGTTCGACGAGTTCGCCGGTAAACATAAAGAACACCTCTGCACGGGTGACGTGAACTACGCCATTGGCTTCTCGTCTGActtcctct                                     >  1:421074‑M2/39‑140 (MQ=255)
                                                                                                        tctcgggatggcgcaccgtggtcgtctgaacgtgctggGTAAAAAACCGCAAGACTTGTTCGACGAGTTCGCCGGTAAACATAAAGAACACCTCGGCACGGGTGACGTGAAATACCACATGGGCTTCTCGTCTGACTTCCAGa                                     >  1:526804‑M2/39‑143 (MQ=255)
                                                                                                                     caccgtggtcggctgagcgggctggGTAAAAATCCGCAAGACGTGTTCGACGAGGTCGCCGGTAAACATAAAGAGCACCTCGGCACGGGTGACGTGAAATACCACATGGGCTTCTCGTCTGACTTCCAGACCGATGGCGGCCt                        <  2:438339‑M2/118‑1 (MQ=255)
                                                                                                                     caccgtgggcggctgagcgtgctggGTGAAAGTCCGCACGACGCGTACGACGCGGTCGCCGGTAAACATAAAGAGCACCTCGGCACGGGTGACGTGAAATACCACATGGGCTTCTCGTCTGACTTCCAGACCGATGGCGGCCt                        <  2:284681‑M2/118‑1 (MQ=255)
                                                                                                                            gtcgtctgaacgtgctggGTAAAAAACCGCAAGACTTGTTCGACGAGTTCGCCGGTAAACATAAAGAACACCTCGGCACGGGTGACGTGAAATACCACATGGGCTTCTCGTCTGACTTCCAGACCGATGGCGGCCTGGTGCAc                 <  1:74138‑M2/125‑1 (MQ=255)
                                                                                                                             tcgtctgaacgtgctggGTAAAAAACCGCAAGACTTGTTCGACGAGTTCGCCGGTAAGCATAAAGAACACCTCGGCACGGGTGACGTGAAATACCACATGGGCTTCTCGTCTGACTTCCAGACCGATGGCGGCCTGGTGCAcc                >  1:413033‑M2/18‑143 (MQ=255)
                                                                                                                                           tggGTAAAAAACCGCAAGACTTGTTCGACGAGTTCGCCGGTAAACATAAAGAACACCTCGGCACGGGTGACGTGAAATACCACATGGGCTTCTCGTCTGACTTCCAGACCGATGGCGGCCTGGTGCACGTGGCGCTGGCGttt  >  1:630244‑M2/4‑143 (MQ=255)
                                                                                                                                              |                                                                                                                                           
AAACGCTTCTCGCTGGAAGGCGGTGACGCGTTAATCCCGATGCTTAAAGAGATGATCCGCCACGCTGGCAACAGCGGCACCCGCGAAGTGGTTCTCGGGATGGCGCACCGTGGTCGTCTGAACGTGCTGGTGAACGTGCTGGGTAAAAAACCGCAAGACTTGTTCGACGAGTTCGCCGGTAAACATAAAGAACACCTCGGCACGGGTGACGTGAAATACCACATGGGCTTCTCGTCTGACTTCCAGACCGATGGCGGCCTGGTGCACCTGGCGCTGGCGTTT  >  NC_000913/759378‑759659

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: