breseq  version 0.32.0  revision 6ff6de7d1b87
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation freq annotation gene description
MC JC 257,908 Δ776 bp 100% [crl] [crl]
RA 374,972 T→C 6.0% intergenic (+91/‑487) mhpE → / → mhpT 4‑hyroxy‑2‑oxovalerate/4‑hydroxy‑2‑oxopentanoic acid aldolase, class I/3‑hydroxyphenylpropionic transporter
RA 449,555 T→A 5.4% I363F (ATC→TTC)  cyoB ← cytochrome o ubiquinol oxidase subunit I
RA 466,365 A→G 4.1% H316H (CAT→CAC ybaE ← putative ABC transporter periplasmic binding protein
RA 635,282 A→G 6.3% I32T (ATC→ACC)  ybdM ← Spo0J family protein, ParB‑like nuclease domain
RA 702,331 Δ1 bp 12.3% coding (421/1149 nt) nagA ← N‑acetylglucosamine‑6‑phosphate deacetylase
RA 702,583 C→A 4.8% D57Y (GAT→TAT)  nagA ← N‑acetylglucosamine‑6‑phosphate deacetylase
RA 891,341 A→T 3.8% D53V (GAC→GTC)  nfsA → nitroreductase A, NADPH‑dependent, FMN‑dependent
RA 1,158,187 G→T 4.4% E107* (GAA→TAA)  ptsG → fused glucose‑specific PTS enzymes: IIB component/IIC component
RA 1,158,377:1 +A 10.6% coding (509/1434 nt) ptsG → fused glucose‑specific PTS enzymes: IIB component/IIC component
JC 1,159,286:1 +GTA 19.2% coding (1418/1434 nt) ptsG → fused glucose‑specific PTS enzymes: IIB component/IIC component
RA 1,290,875 G→A 5.5% G212S (GGC→AGC)  rssB → PcnB‑degradosome interaction factor, response regulator
MC JC 1,299,499 Δ1,199 bp 100% intergenic (+254/‑485) ychE → / → oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein
RA 1,466,201 T→C 7.9% pseudogene (810/2513 nt) ydbA → pseudogene, autotransporter homolog, interrupted by IS2 and IS30
RA 1,665,957 A→G 5.4% T215A (ACG→GCG)  clcB → H(+)/Cl(‑) exchange transporter
RA 1,708,844 C→G 9.1% A642A (GCC→GCG rsxC → SoxR iron‑sulfur cluster reduction factor component, putative membrane‑associated NADH oxidoreductase of electron transport complex
RA 1,726,390 G→T 41.5% R123L (CGC→CTC)  nemR → transcriptional repressor for the nemRA‑gloA operon, quinone‑, glyoxal‑, and HOCl‑activated
RA 2,037,776 T→C 4.5% D126G (GAT→GGT)  yedV ← putative sensory kinase in two‑component regulatory system with YedW
RA 2,043,280 A→T 3.3% intergenic (+382/+188) yodB → / ← serU cytochrome b561 homolog/tRNA‑Ser
JC 2,059,232 Δ3 bp 3.3% coding (457‑459/1488 nt) yeeO ← putative multdrug exporter, MATE family
RA 2,132,787 A→C 100% I204S (ATC→AGC)  wcaA ← putative glycosyl transferase
RA 2,304,796 T→C 9.3% intergenic (+405/+310) eco → / ← mqo ecotin, a serine protease inhibitor/malate dehydrogenase, FAD/NAD(P)‑binding domain
RA 2,411,867 A→G 4.7% I74T (ATC→ACC)  yfbT ← sugar phosphatas
RA 3,051,157 G→A 4.5% R387C (CGC→TGC)  ubiI ← 2‑octaprenylphenol hydroxylase, FAD‑dependent
RA 3,269,954 T→C 13.9% intergenic (+354/+260) yhaC → / ← rnpB pentapetide repeats‑related protein/RNase P, M1 RNA component
RA 3,318,226 G→T 3.4% noncoding (62/77 nt) metY ← tRNA‑Met
RA 3,450,091 G→A 2.9% V47V (GTC→GTT rplV ← 50S ribosomal subunit protein L22
MC JC 3,815,858 Δ82 bp 100% [rph][rph] [rph], [rph]
MC JC 3,909,311 Δ774 bp 100% [pstA][pstC] [pstA], [pstC]
JC 4,001,644 Δ5 bp 100% coding (220‑224/951 nt) corA → magnesium/nickel/cobalt transporter
RA 4,171,647 A→C 100% noncoding (12/120 nt) rrfB → 5S ribosomal RNA of rrnB operon
RA 4,184,542 C→A 100% P1100Q (CCG→CAG)  rpoB → RNA polymerase, beta subunit
RA 4,296,189 A→G 30.2% intergenic (+396/+248) gltP → / ← yjcO glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein
RA 4,296,190 A→C 28.5% intergenic (+397/+247) gltP → / ← yjcO glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein
RA 4,296,267 T→C 16.7% intergenic (+474/+170) gltP → / ← yjcO glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein
RA 4,363,723 T→C 3.1% T441A (ACC→GCC)  dsbD ← thiol:disulfide interchange protein and activator of DsbC

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 4110683 4111540 858 38 [37] [37] 38 [cdh]–tpiA [cdh], tpiA

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 193444 =88 (0.940)3 (0.030) 3/498 NT 3.1% intergenic (+15/‑77) frr/dxr ribosome recycling factor/1‑deoxy‑D‑xylulose 5‑phosphate reductoisomerase
?NC_000913 193489 = 105 (1.160)intergenic (+60/‑32) frr/dxr ribosome recycling factor/1‑deoxy‑D‑xylulose 5‑phosphate reductoisomerase
* ? NC_000913 257908 =NA (NA)54 (0.570) 43/518 NT 43.9% intergenic (+9/‑768) crl/crl pseudogene, sigma factor‑binding protein, RNA polymerase holoenzyme formation stimulator,regulator, Surface structures, transcriptional regulator of cryptic csgA gene for curli surface fibers/pseudogene, sigma factor‑binding protein, RNA polymerase holoenzyme formation stimulator,regulator, Surface structures, transcriptional regulator of cryptic csgA gene for curli surface fibers
?NC_000913 = 700605 69 (0.730)coding (990/1221 nt) nagC N‑acetylglucosamine‑inducible nag divergent operon transcriptional repressor
* ? NC_000913 257908 =NA (NA)107 (1.140) 73/518 NT 100% intergenic (+9/‑768) crl/crl pseudogene, sigma factor‑binding protein, RNA polymerase holoenzyme formation stimulator,regulator, Surface structures, transcriptional regulator of cryptic csgA gene for curli surface fibers/pseudogene, sigma factor‑binding protein, RNA polymerase holoenzyme formation stimulator,regulator, Surface structures, transcriptional regulator of cryptic csgA gene for curli surface fibers
?NC_000913 = 1293039 0 (0.000)intergenic (‑117/‑488) hns/tdk global DNA‑binding transcriptional dual regulator H‑NS/thymidine kinase/deoxyuridine kinase
* ? NC_000913 = 258675NA (NA)33 (0.350) 29/518 NT 31.4% intergenic (+776/‑1) crl/crl pseudogene, sigma factor‑binding protein, RNA polymerase holoenzyme formation stimulator,regulator, Surface structures, transcriptional regulator of cryptic csgA gene for curli surface fibers/pseudogene, sigma factor‑binding protein, RNA polymerase holoenzyme formation stimulator,regulator, Surface structures, transcriptional regulator of cryptic csgA gene for curli surface fibers
?NC_000913 700597 = 72 (0.770)coding (998/1221 nt) nagC N‑acetylglucosamine‑inducible nag divergent operon transcriptional repressor
* ? NC_000913 1207790 =45 (0.480)39 (0.440) 31/486 NT 45.0% coding (290/630 nt) stfP e14 prophage, uncharacterized protein
?NC_000913 1209619 = 53 (0.600)pseudogene (1/501 nt) stfE pseudogene, e14 prophage, side tail fiber protein fragment family,Phage or Prophage Related
* ? NC_000913 = 120780542 (0.450)39 (0.440) 34/486 NT 45.8% coding (305/630 nt) stfP e14 prophage, uncharacterized protein
?NC_000913 = 1209602 53 (0.600)pseudogene (18/501 nt) stfE pseudogene, e14 prophage, side tail fiber protein fragment family,Phage or Prophage Related
* ? NC_000913 1293032 =1 (0.010)107 (1.140) 75/518 NT 99.1% intergenic (‑110/‑495) hns/tdk global DNA‑binding transcriptional dual regulator H‑NS/thymidine kinase/deoxyuridine kinase
?NC_000913 = 1979270 NA (NA)intergenic (‑55/‑483) insA/uspC IS1 repressor TnpA/universal stress protein
* ? NC_000913 = 19785020 (0.000)47 (0.520) 38/502 NT 100% intergenic (‑305/+16) flhD/insB1 flagellar class II regulon transcriptional activator, with FlhC/IS1 transposase B
?NC_000913 1979279 = 0 (0.000)intergenic (‑64/‑474) insA/uspC IS1 repressor TnpA/universal stress protein
* ? NC_000913 1979486 =0 (0.000)45 (0.480) 32/518 NT 100% intergenic (‑271/‑267) insA/uspC IS1 repressor TnpA/universal stress protein
?NC_000913 2101749 = NA (NA)intergenic (‑5/+146) wbbL/insH1 pseudogene, lipopolysaccharide biosynthesis protein/IS5 transposase and trans‑activator
* ? NC_000913 = 19794890 (0.000)86 (0.910) 56/518 NT 100% intergenic (‑274/‑264) insA/uspC IS1 repressor TnpA/universal stress protein
?NC_000913 = 2290111 NA (NA)intergenic (‑32/+1) insH1/yejO IS5 transposase and trans‑activator/pseudogene, autotransporter outer membrane homology,putative transport, Not classified, putative ATP‑binding component of a transport system
* ? NC_000913 2582747 =96 (1.020)3 (0.030) 3/504 NT 2.8% coding (29/1044 nt) ypfG DUF1176 family protein
?NC_000913 2583309 = 112 (1.220)coding (168/576 nt) nudK GDP‑mannose pyrophosphatase