breseq  version 0.33.1  revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation freq annotation gene description
RA 202,885 C→T 5.3% T109M (ACG→ATG)  lpxA → UDP‑N‑acetylglucosamine acetyltransferase
MC JC 257,908 Δ776 bp 100% [crl] [crl]
RA 498,221 T→A 6.8% L56Q (CTG→CAG)  hemH → ferrochelatase
RA 581,394 T→C 6.6% pseudogene (41/309 nt) aaaD → pseudogene; DLP12 prophage; tail fiber assembly protein family;Phage or Prophage Related
RA 839,746 C→T 9.8% E596K (GAA→AAA)  fiu ← catecholate siderophore receptor
RA 987,197 A→T 8.7% intergenic (‑215/+388) ompF ← / ← asnS outer membrane porin 1a (Ia;b;F)/asparaginyl tRNA synthetase
RA 1,076,065 C→T 8.6% A940T (GCA→ACA)  putA ← fused DNA‑binding transcriptional regulator/proline dehydrogenase/pyrroline‑5‑carboxylate dehydrogenase
MC JC 1,299,499 Δ1,199 bp 100% intergenic (+254/‑485) ychE → / → oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein
RA 1,413,542 A→T 100% Y67N (TAT→AAT)  ydaC ← DUF1187 family protein, Rac prophage; putative double‑strand break reduction protein
RA 1,793,897 Δ1 bp 100% coding (213/552 nt) btuE ← glutathione peroxidase
MC JC 1,978,503 Δ776 bp 100% insB1insA insB1, insA
RA 2,093,040 G→A 100% A215T (GCT→ACT)  hisC → histidinol‑phosphate aminotransferase
RA 2,280,025 G→A 8.4% Q153* (CAG→TAG)  rsuA ← 16S rRNA pseudouridine(516) synthase
RA 2,447,744 G→A 8.9% A232V (GCA→GTA)  prmB ← N5‑glutamine methyltransferase
RA 3,511,347 A→G 9.7% V25A (GTC→GCC)  yhfY ← PRD domain protein
RA 4,117,004 T→A 100% D73V (GAT→GTT)  glpK ← glycerol kinase
MC JC 4,188,484 Δ27 bp 100% coding (3136‑3162/4224 nt) rpoC → RNA polymerase, beta prime subunit

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 3423759–3424232 3424529–3424236 5–771 11 [10] [10] 12 [rrfD]–[rrlD] [rrfD],[rrlD]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 226299NA (NA)3 (0.060) 3/272 NT NA noncoding (541/2904 nt) rrlH 23S ribosomal RNA of rrnH operon
?NC_000913 2728632 = NA (NA)noncoding (551/2904 nt) rrlG 23S ribosomal RNA of rrnG operon
* ? NC_000913 1207790 =16 (0.310)31 (0.680) 29/244 NT 72.8% coding (290/630 nt) stfP e14 prophage; uncharacterized protein
?NC_000913 1209619 = 9 (0.200)pseudogene (1/501 nt) stfE pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related
* ? NC_000913 = 120780515 (0.290)27 (0.600) 22/244 NT 70.8% coding (305/630 nt) stfP e14 prophage; uncharacterized protein
?NC_000913 = 1209602 9 (0.200)pseudogene (18/501 nt) stfE pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related
* ? NC_000913 = 304184252 (1.010)3 (0.060) 3/256 NT 5.6% coding (532/981 nt) ygfZ iron‑sulfur cluster repair protein, plumbagin resistance
?NC_000913 = 3041930 52 (1.090)coding (620/981 nt) ygfZ iron‑sulfur cluster repair protein, plumbagin resistance
* ? NC_000913 3110532 =51 (0.990)3 (0.060) 3/254 NT 6.6% intergenic (+93/+56) pheV/yghD tRNA‑Phe/putative membrane‑anchored secretion pathway M‑type protein
?NC_000913 3110563 = 38 (0.800)intergenic (+124/+25) pheV/yghD tRNA‑Phe/putative membrane‑anchored secretion pathway M‑type protein
* ? NC_000913 = 404068269 (1.340)8 (0.160) 3/272 NT 10.9% intergenic (+47/+223) rrfA/mobB 5S ribosomal RNA of rrnA operon/molybdopterin‑guanine dinucleotide biosynthesis protein B
?NC_000913 = 4213161 63 (1.250)intergenic (+3/‑72) rrfE/yjaA 5S ribosomal RNA of rrnE operon/stress‑induced protein