breseq  version 0.33.1  revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation freq annotation gene description
MC JC 257,908 Δ776 bp 100% [crl] [crl]
RA 1,130,375 T→C 10.2% K163K (AAA→AAG flgA ← assembly protein for flagellar basal‑body periplasmic P ring
MC JC 1,299,499 Δ1,199 bp 100% intergenic (+254/‑485) ychE → / → oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein
RA 1,413,542 A→T 100% Y67N (TAT→AAT)  ydaC ← DUF1187 family protein, Rac prophage; putative double‑strand break reduction protein
RA 1,643,967 C→G 18.2% G113A (GGC→GCC)  ydfU ← Qin prophage; uncharacterized protein
RA 1,690,814 G→A 12.9% L321L (CTG→CTA ydgA → DUF945 family protein
RA 1,793,897 Δ1 bp 100% coding (213/552 nt) btuE ← glutathione peroxidase
MC JC 1,978,503 Δ776 bp 100% insB1insA insB1, insA
RA 2,085,352 C→G 15.4% A58A (GCG→GCC yeeE ← UPF0394 family inner membrane protein
RA 2,093,040 G→A 100% A215T (GCT→ACT)  hisC → histidinol‑phosphate aminotransferase
RA 2,394,585 T→C 11.5% I154V (ATC→GTC)  nuoL ← NADH:ubiquinone oxidoreductase, membrane subunit L
RA 2,834,598 C→T 10.0% A230V (GCG→GTG)  norW → NADH:flavorubredoxin oxidoreductase
RA 3,674,690 G→T 8.8% intergenic (+316/‑95) yhjD → / → yhjE inner membrane putative BrbK family alternate lipid exporter/putative MFS transporter; membrane protein
RA 4,117,038 C→A 100% V62L (GTA→TTA)  glpK ← glycerol kinase
RA 4,153,446 A→G 10.5% intergenic (‑349/+249) ppc ← / ← argE phosphoenolpyruvate carboxylase/acetylornithine deacetylase
RA 4,586,255 C→A 8.8% A170S (GCG→TCG)  hsdR ← endonuclease R Type I restriction enzyme

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 366256 366356 101 7 [6] [6] 7 [lacZ] [lacZ]
* * ÷ NC_000913 3423788–3424517 3424517 1–730 7 [6] [5] 8 [rrlD] [rrlD]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 1207790 =11 (0.280)10 (0.290) 6/244 NT 46.8% coding (290/630 nt) stfP e14 prophage; uncharacterized protein
?NC_000913 1209619 = 13 (0.380)pseudogene (1/501 nt) stfE pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related
* ? NC_000913 = 120780511 (0.280)20 (0.580) 20/244 NT 63.8% coding (305/630 nt) stfP e14 prophage; uncharacterized protein
?NC_000913 = 1209602 13 (0.380)pseudogene (18/501 nt) stfE pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related
* ? NC_000913 2785546 =15 (0.390)3 (0.080) 3/268 NT 17.4% intergenic (‑537/+214) ypjC/ileY pseudogene/tRNA‑Ile
?NC_000913 2785568 = 14 (0.370)intergenic (‑559/+192) ypjC/ileY pseudogene/tRNA‑Ile
* ? NC_000913 = 454269131 (0.800)6 (0.170) 3/258 NT 15.4% intergenic (+57/‑425) fimE/fimA tyrosine recombinase/inversion of on/off regulator of fimA/major type 1 subunit fimbrin (pilin)
?NC_000913 = 4542987 37 (1.020)intergenic (+353/‑129) fimE/fimA tyrosine recombinase/inversion of on/off regulator of fimA/major type 1 subunit fimbrin (pilin)