breseq  version 0.33.1  revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
MC JC 257,908 Δ776 bp [crl] [crl]
RA 1,685,527 A→G G354G (GGT→GGC)  fumC ← fumarate hydratase (fumarase C),aerobic Class II
RA 2,145,313 C→A S683Y (TCC→TAC)  yegE → putative diguanylate cyclase
RA 2,173,363 Δ2 bp intergenic (‑1/+1) gatC ← / ← gatC pseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC/pseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC
RA 2,318,095 C→G L594F (TTG→TTC)  rcsC ← hybrid sensory kinase in two‑component regulatory system with RcsB and YojN
RA 3,560,455 +G intergenic (‑2/+1) glpR ← / ← glpR pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon/pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon
MC JC 3,993,337 Δ11 bp coding (2185‑2195/2547 nt) cyaA → adenylate cyclase
RA 4,116,937 T→G K96Q (AAG→CAG)  glpK ← glycerol kinase
RA 4,138,892 G→A V48V (GTC→GTT)  gldA ← glycerol dehydrogenase, NAD+ dependent; 1,2‑propanediol:NAD+ oxidoreductase
RA 4,296,381 +GC intergenic (+587/+55) gltP → / ← yjcO glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 582751 584734 1984 46 [45] [45] 47 [ybcY]–[ompT] [ybcY],ybcY,tfaX,appY,[ompT]
* * ÷ NC_000913 954501 954891 391 47 [45] [45] 46 [ycaO] [ycaO]
* * ÷ NC_000913 1812603 1813396 794 47 [45] [44] 47 [ydjO] [ydjO]
* * ÷ NC_000913 1978328 1979739 1412 46 [45] [44] 46 insB1–insA insB1,insA
* * ÷ NC_000913 2033444 2034145 702 47 [45] [45] 46 [yedR]–[yedS] [yedR],rseX,[yedS]
* * ÷ NC_000913 2192141 2192575 435 47 [45] [44] 46 [yehD]–[yehE] [yehD],[yehE]
* * ÷ NC_000913 2344179 2344602 424 46 [45] [45] 46 yfaL/nrdA adhesin/ribonucleoside‑diphosphate reductase 1, alpha subunit
* * ÷ NC_000913 2994734 2995489 756 46 [45] [45] 46 [ygeK]–[ygeN] [ygeK],[ygeN]
* * ÷ NC_000913 3360718 3361329 612 46 [45] [45] 46 [gltF] [gltF]
* * ÷ NC_000913 3581637 3582325 689 46 [45] [43] 46 [yhhZ] [yhhZ]
* * ÷ NC_000913 4002718 4003413 696 46 [45] [44] 46 [yigF]–[rarD] [yigF],yigG,[rarD]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 3721045 =129 (1.150)300 (2.670) 133/516 0.0 73.3% noncoding (413/1443 nt) IS150 repeat region
?NC_000913 = 3839774 90 (0.800)coding (220/819 nt) nlpA cytoplasmic membrane lipoprotein‑28