Predicted mutation
evidence seq id position mutation annotation gene description
JC JC NC_000913 3,247,848 IS2 (+) +5 bp coding (76‑80/663 nt) yqjA → general envelope maintenance protein; DedA family inner membrane protein; putative multidrug efflux transporter

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 2070271NA (NA)76 (1.160) 51/198 0.3 96.0% noncoding (1/1331 nt) IS2 repeat region
?NC_000913 = 3247852 3 (0.050)coding (80/663 nt) yqjA general envelope maintenance protein; DedA family inner membrane protein; putative multidrug efflux transporter
* ? NC_000913 2996361 =NA (NA)60 (0.920) 45/198 0.6 95.0% noncoding (1331/1331 nt) IS2 repeat region
?NC_000913 3247848 = 3 (0.050)coding (76/663 nt) yqjA general envelope maintenance protein; DedA family inner membrane protein; putative multidrug efflux transporter

TCAATCATCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTA‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913/2070173‑2070271
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑ATGCCAAT‑CATCGATGGATTGGCCAGGGTTTCAAAATCCTGCGCCCACAGGGCTTGCAGCAATTGGGTCAAAAGTTCCATTCATTATTCCTGGTGAATC  <  NC_000913/3247852‑3247754
                                                                                                                                                                                                       
TCAATCATCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAA                                                                                                     <  1:43651/100‑1
TCAATCATCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAA                                                                                                     <  1:1498705/100‑1
TCAATCATCTGTCCTCCAATGAATAGTCTAAAAACTGGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGACATTCAGCGGGCCAGTCTAA                                                                                                     <  1:1342444/100‑1
 CAATCATCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAAT                                                                                                    >  2:1394933/1‑100
   ATCATCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAATGC                                                                                                  >  2:1389666/1‑100
   ATCATCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAATGC                                                                                                  >  1:478700/1‑100
    TCATCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAATGC                                                                                                  >  1:650578/1‑99
    TCATCTGTTCTACAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAATGCC                                                                                                 <  1:1480226/100‑1
        CTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAATGCCAAT‑C                                                                                            >  1:1549989/1‑100
         TGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAATGCCAAT‑CA                                                                                           >  2:502275/1‑100
           TTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAATGCCAAT‑CATC                                                                                         <  2:1233504/100‑1
           TTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAATGCCAAT‑CATC                                                                                         >  2:1489809/1‑100
               CCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAATGCCAAT‑CATCGATG                                                                                     >  1:134448/1‑100
                CAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAATGCCAAT‑CATCGATGG                                                                                    <  1:224154/100‑1
                 nATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAATGCCAAT‑CATCGATGGA                                                                                   >  1:277966/2‑100
                  ATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAATGCCAAT‑CATCGATGGAT                                                                                  >  1:136599/1‑100
                   nGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAATGCCAAT‑CATCGATGGATT                                                                                 >  1:808107/2‑100
                   TGACTAGTCTAAGAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAATGCCAAT‑CATCGATGGATT                                                                                 >  2:1047856/1‑100
                   TGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAATGCCAAT‑CATCGATGGATT                                                                                 <  2:911065/100‑1
                        AGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAATGCCAAT‑CATCGATGGATTGGCCA                                                                            >  2:606596/1‑100
                         GTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAATGCCAAT‑CATCGATGGATTGGCCAG                                                                           >  1:646980/1‑100
                          TCTAAAAACTAGTATTAAGACTATCACTTATTTCAGTGATCTTGATTGTCTGGAGATTCATTGGGCCAGTATAATGGCAAT‑CATCGATGGATTGGCCAG                                                                           >  1:1112242/1‑99
                          TCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAATGCCAAT‑CATCGATGGATTGGCCAGG                                                                          >  1:1573068/1‑100
                           CTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAATGCGAAT‑CATCGATGGATTGGCCAGGG                                                                         >  1:635492/1‑100
                           CTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAATGCCAATCCATCGAT‑GATTGGCattag                                                                         >  1:577461/1‑95
                           CTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAATGCCAAT‑CATCGATGGATTGGCCAGGG                                                                         >  1:241778/1‑100
                           CTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAATGCCAAT‑CATCGATGGATTGGCCAGGG                                                                         >  1:1558464/1‑100
                           CTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAATGCCAAT‑CATCGATGGATTGGCCAGGG                                                                         >  2:1451318/1‑100
                           CTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAATGCCAAT‑CATCGATGGATTGGCCAGGG                                                                         >  2:406254/1‑100
                            TAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAATGCCAAT‑CATCGATGGATTGGCCCGGGT                                                                        >  1:1227736/1‑100
                            TAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAATGCCAAT‑CATCGATGGATTGGCCAGGGT                                                                        <  2:1438848/100‑1
                              AAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAATGCCAAT‑CATCGATGGATTGGCCAGGGTTT                                                                      >  2:1270536/1‑100
                                AACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAATGCCAAT‑CATCGATGGATTGGCCAGGGTTTCA                                                                    <  1:251097/100‑1
                                 ACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAATGCCAAT‑CATCGATGGATTGGCCAGGGTTTCAA                                                                   >  2:147072/1‑100
                                  CTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAATGCCAAT‑CATCGATGGATTGGCCAGGGTTTCAAA                                                                  >  2:959241/1‑100
                                         TAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAATGCCAAT‑CATCGATGGATTGGCCAGGGTTTCAAAATCCTGC                                                           <  1:1012587/100‑1
                                         TAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCAAGTCTAATGCCAAT‑CATCGATGGATTGGCCAGGGTTTCAAAATCCTGC                                                           >  1:889977/1‑100
                                          accaaTATCACTTAAATAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAATGCCAAT‑CATCGATGGATTGGCCAGGGTTTCAAAATCCTGCG                                                          >  2:1484037/6‑100
                                          AAGACTATCACTGATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAATGCCAAT‑CATCGATGGATTGGCCAGGGTTTCAAAATCCTGCG                                                          <  1:1307667/100‑1
                                              CTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAATGCCAAT‑CATCGATGGATTGGCCAGGGTTTCAAAATCCTGCGCCCA                                                      >  1:1376483/1‑100
                                              CTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAATGCCAAT‑CATCGATGGATTGGCCAGGGTTTCAAAATCCTGCGCCCA                                                      >  2:229923/1‑100
                                                ATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAATGCCAAT‑CATCGATGGATTGGCCAGGGTTTCAAAATCCTGCGCCCACA                                                    >  1:833093/1‑100
                                                  cacttaaaTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAATGCCAAT‑CATCGATGGATTGGCCAGGGTTTCAAAATCCTGCGCCCACAGG                                                  >  1:429061/9‑100
                                                     nTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAATGCCAAT‑CATCGATGGATTGGCCAGGGTTTCAAAATCCTGCGCCCACAGGGCT                                               >  2:917652/2‑100
                                                     TTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAATGCCAAT‑CATCGATGGCTTGGCCAGGGTTTCAAAATCCTGCGCCCACAGGGCT                                               <  2:1129304/100‑1
                                                       ATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAATGCCAAT‑CATCGATGGATTGGCCAGGGTTTCAAAATCCTGCGCCCACAGGGCTTG                                             <  2:134450/100‑1
                                                        TTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAATGCCAAT‑CATCGATGGATTGGCCAGGGTTTCAAAATCCTGCGCCCACAGGGCTTGC                                            <  1:364576/100‑1
                                                        TTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAATGCCAAT‑CATCGATGGATTGGCCAGGGTTTCAAAATCCTGCGCCCACAGGGCTTGC                                            <  1:757262/100‑1
                                                         TTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAATGCCAAT‑CATCGATGGATTGGCCAGGGTTTCAAAATCCTGCGCCCACAGGGCTTGCA                                           <  2:780771/100‑1
                                                          TAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAATGCCAAT‑CATCGATGGATTGGCCAGGGTTTCAAAATCCTGCGCCCACAGGGCTTGCA                                           <  1:380831/99‑1
                                                           AAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAATGCCAAT‑CATCGATGGATTGGCCAGGGTTTCAAAATCCTGCGCCCACAGGGCTTGCAGC                                         <  2:146461/100‑1
                                                              TGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAATGCCAAT‑CATCGATGGATTGGCCAGGGTTTCAAAATCCTGCGCCCACAGGGCTTGCAGCAAT                                      >  1:1359147/1‑100
                                                                    TGGTTGTCTGGAGATTCAGGGGGCCAGTCTAATGCCAAT‑CATCGATGGATTGGCCAGGGTTTCAAAATCCTGCGCCCACAGGGCTTGCAGCAATTGGGTC                                <  2:441512/100‑1
                                                                        TGTATGGAGATTCAGGGGGCCAGTCTAATGCCAAT‑CATCGATGGATTGGCCAGGGTTTCAAAATCCTGCGCCCACAGGGCTTGCAGCAATTGGGTAAAAA                            >  2:1526550/1‑100
                                                                         GTCTGGAGATTCAGGGGGCCAGTCTAATGCCAAT‑CATCGATGGATTGGCCAGGGTTTCAAAATCCTGCGCCCACAGGGCTTGCAGCAATTGGGTCAAAAG                           <  2:384499/100‑1
                                                                            TGGAGATTGAGGGGGCCAGTCTAATGCCAAT‑CATCGATGGATTGGCCAGGGTGTCAAAATCCTGCGCCCACAGGGCTTGCAGCAATTGGGTCAAAAGTTC                        >  1:692228/1‑100
                                                                             GGAGATTCAGGGGGCCAGTCTAATGCCAAT‑CATCGATGGATTGGCCAGGGTTTCAAAATCCTGCGCCCACAGGGCTTGCAGCAATTGGGTCAAAAGTTCC                       >  2:273412/1‑100
                                                                             GGAGATTCAGGGGGCCAGTCTAATGCCAAT‑CATCGATGGATTGGCCAGGGTTTCAAAATCCTGCGCCCACAGGGCTTGCAGCAATTGGGTCAAAAGTTCC                       <  2:520631/100‑1
                                                                             GGAGATTCAGGGGGCCAGTCTAATGCCAAT‑CATCGATGGATTGGCCAGGGTTTCAAAATCCTGCGCCCACAGGGCTTGCAGCAATTGGGTCAAAAGTTCC                       >  2:986269/1‑100
                                                                             GGAGATTCAGGGGGCCAGTCTAATGCCAAT‑CATCGATGGATTGGCCAGGGTTTCAAAATCCTGCGCCCACAGGGCTTGCAGCAATTGGGTCAAAAGTTCC                       >  1:1554184/1‑100
                                                                             GGAGATTCAGGGGGCCAGTCTAATGCCAAT‑CATCGATGGATTGGCCAGGGTTTCAAAATCCTGCGCCCACAGGGCTTGCAGCAATTGGGTCAAAAGTTCC                       <  1:399573/100‑1
                                                                                  TTCAGGGGGCCAGTCTAATGCCAAT‑CATCGATGGATTGGCCAGGGTTTCAAAATCCTGCGCCCACAGGGCTTGCAGCAATTGGGTCAAAAGTTCCATTCn                  <  2:277969/100‑2
                                                                                  TTCAGGGGGCCAGTCTAATGCCAAT‑CATCGATGGATTGGCCAGGGTTTCAAAATCCTGCGCCCACAGGGCTTGCAGCAATTGGGTCAAAAGTTCCATTCA                  >  2:640775/1‑100
                                                                                   TCAGGGGGCCAGTCTAATGCCAAT‑CATCGATGGATTGGCCAGGGTTTCAAAATCCTGCGCCCACAGGGCTTGCAGCAATTGGGTCAAAAGTTCCATTCAT                 <  2:1480306/100‑1
                                                                                   TCAGGGGGCCAGTCTAATGCCAAT‑CATCGATGGATTGGCCAGGGTTTCAAAATCCTGCGCCCACAGGGCTTGCAGCAATTGGGTCAAAAGTTCCATTCAT                 >  1:1419161/1‑100
                                                                                   TCAGGGGGCCAGTCTAATGCCAAT‑CATCGATGGATTGGCCAGGGTTTCAAAATCCTGCGCCCACAGGGCTTGCAGCAATTGGGTCAAAAGTTCCATTCAT                 <  1:1389652/100‑1
                                                                                    CAGGGGGCCAGTCTAATGCCAAT‑CATCGATGGATTGGCCAGGGTTTCAAAATCCTGCGCCCACAGGGCTTGCAGCAATTGGGTCAAAAGTTCCATTCATT                <  2:1359161/100‑1
                                                                                     AGGGGGCCAGTCTAATGCCAAT‑CATCGATGGATTGGCCAGGGTTTCAAAATCCTGCGCCCACAGGGCTTGCAGCAATTGGGTCAAAAGTTCCATTCATTA               >  2:103553/1‑100
                                                                                       GGGGCCAGTCTAATGCCAAT‑CATCGATGGATTGGCCAGGGTTTCAAAATCCTGCGCCCACAGGGCTTGCAGCAATTGGGTCAAAAGTTCCATTCATTATT             >  2:1240737/1‑100
                                                                                       GGGGCCAGTCTAATGCCAAT‑CATCGATGGATTGGCCAGGGTTTCAAAATCCTGCGCCCACAGGGCTTGCAGCAATTGGGTCAAAAGTTCCATTCATTATT             <  2:136601/100‑1
                                                                                         GGCCAGTCTAATGCCAAT‑CATCGATGGATTGGCCAGGGTTTCAAAATCCTGCGCCCACAGGGCTTGCAGCAATTGGGTCAAAAGTTCCATTCATTATTCC           <  2:478704/100‑1
                                                                                         GGCCAGTCTAATGCCAAT‑CATCGATGGATTGACCAGGGTTTCAAAATCCTGCGCCCACAGGGCTTGCAGCAATTGGGTCAAAAGTTCCATTCATTATTCC           <  1:765335/100‑1
                                                                                          GCCAGTCTAATGCCAAT‑CATCGATGGATTGGCCAGGGTTTCAAAATCCTGCGCCCACAGGGCTTGCAGCAATTGGGTCAAAAGTTCCATTCATTATTCCT          >  2:1457022/1‑100
                                                                                           CCAGTCTAATGCCAAT‑CATCGATGGATTGGCCAGGGTTTCAAAATCCTGCGCCCACAGGGCTTGCAGCAATTGGGTCAAAAGTTCCATTCATTATTCCTG         <  2:429065/100‑1
                                                                                               TCTAATGCCAAT‑CATCGATGGATTGGCCAGGGTTTCAAAATCCTGCGCCCACAGGGCTTGCAGCAATTGGGTCAAAAGTTCCATTCATTATTCCTGGTGA     >  1:1294955/1‑100
                                                                                                  AATGCCAAT‑CATCGATGGATTGGCCAGGGTTTCAAAATCCTGCGCCCACAGGGCTTGCAGCAATTGGGTCAAAAGTTCCATTCATTATTCCTGGTGAATC  <  1:1360948/100‑1
                                                                                                                                                                                                       
TCAATCATCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTA‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913/2070173‑2070271
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑ATGCCAAT‑CATCGATGGATTGGCCAGGGTTTCAAAATCCTGCGCCCACAGGGCTTGCAGCAATTGGGTCAAAAGTTCCATTCATTATTCCTGGTGAATC  <  NC_000913/3247852‑3247754

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.