breseq  version 0.29.0  revision 8f9c342918e4
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
MC JC 257,908 Δ776 bp [crl] [crl]
MC JC 1,681,637 (TTAC)3→2 coding (662‑665/723 nt) folM → dihydromonapterin reductase, NADPH‑dependent; dihydrofolate reductase isozyme
RA 1,756,553 A→T K286* (AAA→TAA)  pykF → pyruvate kinase I
JC JC 1,979,486 IS5 (+) +4 bp intergenic (‑271/‑264) insA ← / → uspC IS1 repressor TnpA/universal stress protein
RA 2,173,363 Δ2 bp intergenic (‑1/+1) gatC ← / ← gatC pseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC/pseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC
RA 2,433,040 G→C P296R (CCG→CGG)  accD ← acetyl‑CoA carboxylase, beta (carboxyltransferase) subunit
RA 2,754,639 C→A Q42H (CAG→CAT ratA ← toxic UPF0083 family protein inhibitor of 70S ribosome formation
RA 2,810,760 T→C intergenic (+81/‑10) ygaH → / → mprA putative L‑valine exporter, norvaline resistance protein/transcriptional repressor of microcin B17 synthesis and multidrug efflux
RA 3,248,432 (G)5→6 coding (660/663 nt) yqjA → general envelope maintenance protein; DedA family inner membrane protein; putative multidrug efflux transporter
RA 3,338,928 C→A L107F (TTG→TTT mlaE ← ABC transporter maintaining OM lipid asymmetry, inner membrane permease protein
RA 3,560,455 +G intergenic (‑2/+1) glpR ← / ← glpR pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon/pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon
JC JC 3,793,843 IS2 (–) +5 bp intergenic (‑160/‑140) yibB ← / → hldD YibB family protein, function unknown/ADP‑L‑glycero‑D‑mannoheptose‑6‑epimerase, NAD(P)‑binding
MC JC 3,815,859 Δ82 bp [rph][rph] [rph], [rph]
RA 3,882,550 G→A L394F (CTC→TTC)  dnaA ← chromosomal replication initiator protein DnaA, DNA‑binding transcriptional dual regulator
RA 4,189,448 C→T Q1367* (CAG→TAG)  rpoC → RNA polymerase, beta prime subunit
RA 4,191,997 G→C P213P (CCC→CCG thiG ← thiamine biosynthesis ThiGH complex subunit
RA 4,196,204 G→A intergenic (‑105/+128) thiC ← / ← rsd phosphomethylpyrimidine synthase/stationary phase protein, binds sigma 70 RNA polymerase subunit
RA 4,296,381 +GC intergenic (+587/+55) gltP → / ← yjcO glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 3423691–3424234 3424608–3424238 5–918 93 [90] [92] 94 [rrfD]–[rrlD] [rrfD], [rrlD]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 1207790 =22 (0.140)84 (0.650) 61/166 1.6 79.1% coding (290/630 nt) stfP e14 prophage; uncharacterized protein
?NC_000913 1209619 = 26 (0.200)pseudogene (1/501 nt) stfE pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related
* ? NC_000913 = 120780523 (0.150)103 (0.800) 68/166 0.9 82.0% coding (305/630 nt) stfP e14 prophage; uncharacterized protein
?NC_000913 = 1209602 26 (0.200)pseudogene (18/501 nt) stfE pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related
* ? NC_000913 = 12994980 (0.000)180 (1.220) 98/190 0.1 100% intergenic (+253/‑1684) ychE/oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein
?NC_000913 1300698 = 0 (0.000)intergenic (+1453/‑484) ychE/oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein
* ? NC_000913 = 19785020 (0.000)167 (1.180) 103/182 0.0 100% intergenic (‑305/+16) flhD/insB1 flagellar class II regulon transcriptional activator, with FlhC/IS1 transposase B
?NC_000913 1979279 = 0 (0.000)intergenic (‑64/‑474) insA/uspC IS1 repressor TnpA/universal stress protein