breseq  version 0.27.1  revision 87c22d663cc3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
MC JC 257,908 Δ776 bp [crl] [crl]
RA 363,400 Δ1 bp coding (2906/3075 nt) lacZ ← beta‑D‑galactosidase
JC JC 889,489 IS5 (+) +4 bp coding (328‑331/1686 nt) ybjL ← putative transporter
RA 1,151,759 G→A E49K (GAA→AAA)  acpP → acyl carrier protein (ACP)
RA 1,307,744 Δ1 bp coding (902/1461 nt) clsA ← cardiolipin synthase 1
JC 1,673,557 Δ120 bp intergenic (‑56/‑237) mdtJ ← / → tqsA multidrug efflux system transporter/pheromone AI‑2 transporter
JC 1,674,769 Δ12 bp coding (857‑868/1035 nt) tqsA → pheromone AI‑2 transporter
MC JC 1,978,503 Δ776 bp insB1insA insB1, insA
RA 2,173,363 Δ2 bp intergenic (‑1/+1) gatC ← / ← gatC pseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC/pseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC
RA 2,610,107 G→A E541K (GAG→AAG)  hyfG → hydrogenase 4, subunit
RA 3,247,487 +A intergenic (+59/‑286) exuR → / → yqjA hexuronate regulon transcriptional repressor; autorepressor/general envelope maintenance protein; DedA family inner membrane protein; putative multidrug efflux transporter
JC JC 3,393,816 IS5 (–) +4 bp coding (2440‑2443/3801 nt) yhdP ← DUF3971‑AsmA2 domains protein
RA 3,473,612 A→C L157* (TTA→TGA)  rpsG ← 30S ribosomal subunit protein S7
RA 3,560,455 +G intergenic (‑2/+1) glpR ← / ← glpR pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon/pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon
RA 4,187,667 T→A F773Y (TTC→TAC)  rpoC → RNA polymerase, beta prime subunit
RA 4,213,051 A→C noncoding (12/120 nt) rrfE → 5S ribosomal RNA of rrnE operon
RA 4,296,381 +GC intergenic (+587/+55) gltP → / ← yjcO glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 3423652 3424569–3423662 11–918 295 [291] [291] 297 [thrV]–[rrlD] [thrV], rrfD, [rrlD]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 11041155 (0.010)363 (0.830) 202/298 0.2 96.5% coding (165/456 nt) csgB curlin nucleator protein, minor subunit in curli complex
?NC_000913 1104420 = 21 (0.050)intergenic (+14/‑27) csgB/csgA curlin nucleator protein, minor subunit in curli complex/curlin subunit, amyloid curli fibers, cryptic
* ? NC_000913 = 12994980 (0.000)427 (1.000) 215/292 0.0 100% intergenic (+253/‑1684) ychE/oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein
?NC_000913 1300698 = 0 (0.000)intergenic (+1453/‑484) ychE/oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein